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EFEKTIVITAS INFUSA KULIT JERUK PURUT (Citrus hystrix DC.) TERHADAP PERTUMBUHAN Candida albicans PENYEBAB SARIAWAN SECARA in vitro Khafidhoh, Zakiyatul; Dewi, Sri Sinto; Iswara, Arya
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Student Paper Presentation The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Candida albicans is a normal flora in the oral mucosa, tongue, and palate, but it could become the pathogenic one. If the amounts were excess so that it could cause thrush. Kaffir lime peel infusion contains saponins, tannins, flavonoids, coumarin which are active antifungal compound. The purpose of this study was to examine the effectiveness of kaffir lime peel infusion to the growth of Candida albicans which causes thrush with a consentration of 10%, 15%, 20% and contact time of 5 minutes, 10 minutes, 15 minutes. Method of this study used an experimental research laboratory. Suspension Candida albicans 100 mL with dilution 10-4 of McFarland 0,5 and contacted to the kaffir lime peel infusion 10%, 15%, 20% with three repetitions. After 5 minutes, 10 minutes, and 15 minutes put 100 μL and then inoculated into SGA antibiotics then incubated at 37˚C for 48 hours. Based on the results of this study concluded that kaffir lime peel infusion can inhibit the growth of Candida albicans. The best results was the 20% consentration and contact time of 15 minutes with an average colonies of 3×104 CFU/100 μL. The higher consentration of kaffir lime peel infusion the more able to inhibit the growth of Candida albicans and the longer the contact time kaffir lime peel infusion, the more can inhibit the growth of Candida albicans.Keywords: Infusion, Citrus hystrix DC., Candida albicans.
HAEMAGGLUTINATION ACTIVITY OF Salmonella typhi FLAGELLIN PROTEIN BASED ON ABO BLOOD GROUP Saptaningtyas, Ragil; Darmawati, Sri; Dewi, Sri Sinto
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Student Paper Presentation The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Salmonella typhi is a negative basil Gram bacterium that causes typhoid fever. Flagel of S. typhi contain proteins, as locomotor and it can help bacteria to attach on host cells. Haemagglutinin protein is protein that can agglutinate erythrocytes. The goal of this research is to analize S. typhi flagellin proteins haemagglutination activity Based on ABO blood group. Flagellin proteins isolation method of modified Alexan method (2009) and haemagglutination test method of Finkeltein and Hanne method (1982). Haemagglutination result of ABO blood type show that SLT-1 S. typhi flagellin proteins can’t agglutinate human erythrocytes A, B, and O, but it can agglutinate human erythrocytes until 8 times dilution of AB blood type from 50 μg/μl concentration in 50 μl. BA 07.4 S. typhi flagellin proteins can agglutinate human erythrocytes of A blood type until 16 times dilution, 8 times dilution of AB blood type, 4 times dilution of O blood type, and it can’t agglutinate human erythrocytes B blood type.Keywords: Salmonella typhi, Flagellin Proteins, Haemagglutination, Erythrocytes of ABO System.
DAYA HAMBAT INFUSA BIJI PINANG (Areca catechu L.) TERHADAP BAKTERI Staphylococcus aureus Wael, Maryam Ulfah; Dewi, Sri Sinto; Maharani, Endang Tri Wahyuni
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Pendidikan, Sains dan Teknologi
Publisher : Universitas Muhammadiyah Semarang

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Abstract

The objective of this research is to measure and analyze the difference of inhibition concentration of areca nuts concentration 2%b/v, 3%b/v, 4%b/v,5%b/v and 6%b/v on the growth of S.aureus. The sample used in this research isareca nuts (Areca catechu L.) with the object of research S.aureus that hasequalized turbidity with Mc Farland standard 0.5. Antibacterial testing is doneby diffusion method (wells). The results showed that areca nuts can inhibitS.aureus bacteria with area of inhibit zone for concentration 2% b/v, 3% b/v,4% b/v, 5% b/v and 6% b/v is 14,5 mm, 14,6 mm, 16 mm, 18 mm and 18 mm.Result of normality test Kolmogorov-Smirnov S.aureus (p=0,691) the valuesobtained are normally distributed. The homogenity test of S.aureus is nothomogeneous, Then proceed with non parametric test that is Kruskal Wallis(p=1,000) the values obtained showed no significant difference to meaninhibition zone diameter between concentrations 2%b/v, 3%b/v, 4%b/v, 5%b/vand 6%b/v areca nuts infusion against S. aureus bacteria. Keywords: Areca nuts infusion, Staphylococcus aureus, Inhibition
VIABILITAS BAKTERI ASAM LAKTAT ASAL ASI TERHADAP pH ASAM LAMBUNG DAN GARAM EMPEDU Dewi, Sri Sinto; Anggraini, Herlisa
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2012: SEMINAR NASIONAL HASIL PENELITIAN 2012
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Air susu ibu merupakan salah satu sumber bakteri asam laktat, yang berfungsi untuk menjagakeseimbangan mikroflora saluran pencernaan dan meningkatkan sistem kekebalan. Bakteri asamlaktat sebagai probiotik berpeluang hidup pada saluran pencernaan jika berasal dari tubuh manusia,mempunyai ketahanan terhadap pH asam lambung dan garam empedu. Tujuan penelitian ini adalahseleksi dan isolasi bakteri asam laktat dari air susu ibu yang tahan terhadap pH dan garam empedu.Penelitian menggunakan 6 isolat bakteri asam laktat yang telah diisolasi dari air susu ibu. Masingmasingisolat diuji ketahanan terhadap pH rendah 1,5 sampai 6 dan garam empedu konsentrasi 1-5%.Hasil yang diperoleh terdiri dari 6 isolat bakteri asam laktat yang termasuk dalam genus Lactobacillusyang memiliki ketahanan terhadap pH rendah dan garam empedu. Isolat yang berpotensi untukdikembangkan sebagai probiotik sistem kekebalan adalah Lactobacillus isolat A.1 dan A.2
WAKTU PAPARAN GAS OZON TERHADAP PERTUMBUHAN BAKTERI Escherichia coli Dewi, Sri Sinto; Wardoyo, Fandhi Adi; Ma’ruf, Amal
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Pendidikan, Sains dan Teknologi
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Ozone is one of the gases that can be used as a disinfectant to inhibit the growth of Escherichia coli bacteria. Time of exposure to ozone gas is a way used to disinfectEscherichia coli bacteria. The purpose of this study was to observe the number ofEscherichia coli bacteria exposed to ozone gas with exposure time of 5, 10, 15, 20, 25and 30 minutes and ozone gas control. The study sample was Escherichia colibacteria exposed to ozone gas. The results of the number of colonies of Escherichiacoli on CCA media after exposure to 5, 10, 15 minutes of ozone gas were mean 2.5 x106, 1.25 x 106, 0.5 x 106 CFU / ml, and 20, 25, 30 minutes there is no bacterialgrowth. Control or no exposure to ozone gas mean is 9 x106 CFU / ml. The time ofexposure to ozone gas is most effective in killing Escherichia coli bacteria ie at 20minutes.Keywords: ozone gas, Escherichia coli, Exposure time.
DETECTION OF VIRULENCE GENES PHOP AND PHOQ IN SALMONELLA SPP. USING IN SILICO POLYMERASE CHAIN REACTION Ethica, Stalis Norma; Fuad, Hayatun; Hidayah, Nur; Dewi, Sri Sinto; Ernanto, Aditya Rahman; Sulistyaningtyas, Ayu Rahmawati; Silvestre, Richard David; Darmawati, Sri
RESEARCH FAIR UNISRI Vol 4, No 1 (2020): RESEARCH FAIR UNISRI
Publisher : RESEARCH FAIR UNISRI

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Abstract

Detection of Salmonella bacteria based on their virulence genes is among essential steps in the eradication of clinical infection by bacteria. In this study, two pair of primers, PhoPF-PhoPR: 5?- CCGCGCAGGAAAAACTCAAA-3? and 5?-ATCTGTTCCAGCATCACCGG -3? as well as PhoQF-PhoQR: 5?-AGAGATGATGCGCGTACTGG-3? and 5?- CAGACGCCCCATGAGAACAT-3?, had been successfully designed using Primer3Plus to detect the presence of phoP and phoQ genes in Salmonella spp. Using genomic DNA of 44 genomic data of Salmonella spp. as templates, PhoPF-PhoPR could produce 520-bp amplicon, while PhoQF-PhoQR could result in 598-bp amplicon. Results of in silico PCR showed that both pairs of primers PhoPF-PhoPR and PhoQF-PhoQR could detect only Salmonella enterica species, and no Salmonella bongori species could be detected based on phoP and phoQ sequences. Both pairs of PhoPF-PhoPR and PhoQF-PhoQR primers were also able to detect the virulence genes in most of the studied subspecies of Salmonella enterica available in silico database unless Arizona subspecies. As conclusion, based on this in silico study, phoP and phoQ genes appeared to be biomarkers for Salmonella enterica species. Both pairs of primers designed in this study has potential to be used as detection tool to differentiate species Salmonella enterica from Salmonella bongori, and also to distinguish S.enterica subsp. enterica from subsp. Arizonae.Keywords: Gene detection, bacterial virulence, phoP, phoQ, Salmonella spp.
PROFIL PROTEIN BAKTERI ASAM LAKTAT ISOLAT AIR SUSU IBU Dewi, Sri Sinto; Ariyadi, Tulus
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: PROSIDING IMPLEMENTASI PENELITIAN PADA PENGABDIAN MENUJU MASYARAKAT MANDIRI BERKEMAJUAN
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Air susu ibu (ASI) merukapan makanan pertama yang diberikan untuk bayi yang baru lahir, pada ASI ditemukan bakteri asam laktat yang mampu menjaga keseimbangan mikroflora saluran pencernaan dari bakteri patogen. Penelitian ini bertujuan ingin mengetahui profil protein bakteri asam laktat isolat ASI yang mempunyai viabilitas terhadap asam dan empedu dengan menggunakan metode SDS-PAGE. Tahap penelitian meliputi isolasi bakteri asam laktat, aktivasi kultur bakteri asam laktat, isolasi protein, separasi protein dan perhitungan berat molekul. Kultur yang digunakan adalah bakteri asam laktat isolate ASI. Hasil identifikasi bakteri asam laktat adalah Pediococcus acidilactic dan Lactobacillus plantarum,Lactobacillus acidophilus. Separasi menunjukkan bakteri asam laktat isolat ASI mempunyai pita mayor dengan berat molekul 55 kDA dan 43 kDA serta mempunyai pita minor yang bervariasi dengan berat molekul yang paling rendah 8 kDA.Kata kunci : BAL ASI, Protein, SDS-PAGE
SURVEI KEJADIAN FILARIASIS PADA MASYARAKAT DESA TAWANGREJO KUNDURAN BLORA Sumanto, Didik; Dewi, Sri Sinto
JURNAL LITBANG Vol 4, No 3 (2006): Sain dan Teknologi
Publisher : JURNAL LITBANG

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Abstract

Background : On April 2005, DKK Blora found fourteen peoples that infected by elephanthiasis (/ilariasis) in Blora Central of Jawa. Tofollow up this discovery, DKK Blora do thefilariasis surveyfor 500 peoples andfound 163 peoples (48 o/o) wilh posilif filariasis infection.Objective : To do the repetition survey and tell the filariasis incident on society of Tawangrejo, Kunduran, Blora.Research method : The population of this resesrh is all of the people of Tawangrejo Blora" Accidental sampling tecnique applied in this research.Result: This researchfound 5 peoples (2,1 o/o) with positif filariasis infection.One of them infected by Wuchereria bancrofty andfour peoples infected by Brugia malayi.Conclusion : The saurce of infection assumpted from the people that come from external of Jawa with the elephantiasis condition. The incident rate offilariasis in Tawangrejo on November 2005 is 2,17 %. This rale is not too high with the early assumption that to richfor 48 %.Key words : survey, elephanthiasis, filariasis
SOCIALIZATION OF THE BENEFITS OF FERMENTING CATTLE MILK INTO YOGURT AS A PROBIOTIC FOOD PRODUCT FOR HOUSEWIFE COMMUNITY OF SRUNI VILLAGE, MUSUK, BOYOLALI Dewi, Sri Sinto; Ethica, Stalis Norma; Hersoelistyorini, Wikanastri
Jurnal Pengabdian Pada Masyarakat Vol 4 No 4 (2019)
Publisher : Universitas Mathla'ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (991.603 KB) | DOI: 10.30653/002.201944.178

Abstract

Boyolali Regency is among districts in Indonesia, which still has poverty issues and receives direct cash assistance from the government. Yet, villages of the regency including Sruni at Musuk sub-district has been known as one of the main producers of fresh cow milk for the Central Java region.There has been no attempt to process fresh milk into food products of higher economic value at Sruni Village. Meanwhile, results of the strengths, weaknesses, opportunities, and threats (SWOT) analysis at Musuk showed that the region has the potential to be developed for dairy industry. Therefore, through socialization program, community empowerment should be initiated by socializing benefits of fermenting cattle milk into yogurt as a probiotic food product. The socialization had been carried out for 12 housewives in the village of Sruni through two small-class seminars in April 2019. Evaluation was conducted by comparing the number of correct answers from participants? answers recorded on questionnaire given prior and after each of both seminars. Percentage of improved answers were presented in histograms and then analyzed. As results, the first seminar produced in average 47.4% improved answers, while the second seminar could generate in average 27.3% improved answers. The results showed that in general, the conducted socialization program was quite successful in improving understanding of Sruni villagers on the benefits of fermenting cattle milk into yogurt as a probiotic food product.
Bacterial Plasmids Profile from Escherichia coli Resistant to Metronidazole and Nalidixic Acid Iswara, Arya; Dewi, Sri Sinto
El-Hayah Vol 6, No 1 (2016): EL-HAYAH (VOL 6, NO 1,September 2016)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (494.55 KB) | DOI: 10.18860/elha.v6i1.4079

Abstract

bacteria that cause an illness. Antibiotic treatments to a patient have a purpose to eliminate the pathogen bacteria. Bacteria resistance to antibiotic was influenced by the intensity of antibiotic treatment in a region, the uncontrolled antibiotics treatments would increase the antibiotic resistance of bacteria. Plasmids was an extrachromosomal DNA that encodes a functional protein that would eliminate the antibiotic activity. Plasmid is the determinant of bacteria sensitivity to antibiotics. In this case it would be important to find out the bacterial plasmid profile on the E.coli resistant to metronidazole and nalidixic acid antibiotics. This research was using four different sample from faces of diarrhea, ice block, waters from well, and ketchup to cultivate the E. coli. lasmid isolation method was carried out by lyses alkali method. Plasmid profile of the E. coli that resistant to metronidazole and nalidixic acid antibiotics and analyzed using electrophoresis on 1% agarose. E. coli plasmid DNA profile was observed as a fluorescent DNA band in ultraviolet rays. In result, isolated plasmids from bacteria that resistant to antibiotics metronidazole and nalidixic acid having similar size approximately 500 bp, different from bacteria that sensitive to antibiotics metronidazole and nalidixic acid has a smaller size in region of 100 bp.