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PERTUMBUHAN DAN HASIL BAWANG MERAH ASAL UMBI TSS VARIETAS TUK TUK PADA UKURAN DAN JARAK TANAM YANG BERBEDA Darma, Wika Anrya; Susila, Anas Dinurrohman; Dinarti, Diny
Agrovigor 2015: Vol 8, No 2 (2015) SEPTEMBER
Publisher : University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (314.739 KB) | DOI: 10.21107/agrovigor.v0i0.980

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Penelitian ini dilaksanakan mulai Oktober 2014 hingga Februari 2015 di Green House Kebun Percobaan IPB Cikabayan, Dramaga, Bogor. Penelitian ini bertujuan memperoleh ukuran umbi asal TSS dan jarak tanam yang optimal dalam memproduksi umbi benih bawang merah. Rancangan penelitian menggunakan Rancangan Acak Lengkap dua faktor. Faktor pertama yaitu ukuran umbi yang terdiri dari umbi berukuran sedang (diameter= 1.5-1.8 atau berbobot 5-10 g) dan besar (diameter= 1.8 cm atau berbobot 10 g). Faktor kedua yaitu jarak tanam yang terdiri dari jarak tanam 20x20 cm dan 30x30 cm. Umbi yang digunakan dalam percobaan ini merupakan umbi hasil penanaman dari biji TSS yang telah disimpan selama dua bulan. Hasil penelitian menunjukkan bahwa penggunaan umbi berukuran sedang dan besar tidak memberikan pengaruh yang nyata terhadap produksi bawang merah. Sedangkan jarak tanam memberikan hasil yang berbeda nyata. Penggunaan umbi berukuran sedang pada jarak tanam yang lebih rapat dapat meminimalkan biaya produksi dari segi penyediaan umbi benih..Kata kunci: Bawang merah, jarak tanam, ukuran umbi, umbi benih asal TSS
EMBRIOGENESIS DAN DESIKASI EMBRIO SOMATIK JERUK KEPROK BATU 55 (CITRUS RETICULATA BLANCO.) UNTUK MENINGKATKAN FREKUENSI PERKECAMBAHAN Fathur Rahmi, Atika; Purwito, Agus; Husni, Ali; Dinarti, Diny
Jurnal Hortikultura Indonesia Vol. 8 No. 2 (2017): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (381.578 KB) | DOI: 10.29244/jhi.8.2.79-87

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ABSTRACTIn vitro breeding technique of citrus is effective when optimum explant regeneration method is obtained. Low germination frequency and high abnormality were barrier in citrus somatic embryogenesis. This research aimed at optimizing somatic embryogenesis in Tangerine var. Batu 55. This research consisted of 3 experiments. First experiment was maturation of embryogenesis, using Completely Randomized Design (CRD) method. Modified MS+MW was used as basic media added with 500 mg L-1 malt extract (control) and addition of 3 mg L-1 BAP, and 2.5 mg L-1 ABA as treatments. Second experiment was SE (cotyledonary phage) desiccation. Factorial CRD used in two factors. First factor was poly-ethylene-glicol/PEG 8000 (0, 2.5, 5, 7.5 and 10%), while second factor was immersed periods (control, 3, 6, and 9 hours), in desiccant solution (base medium + PEG). Third experiment was studied of plantlet growth and development planlets. Based on CRD 2 factor method, the first factor was PEG concentrations from the second experiment. Second factor were active charcoal treatments (with or without), in basic media. The result showed that 2.5 mg L-1 ABA produced has highest mature somatic embryo (SE). Desiccation for 9 hours, induced the highestt germination frequencies (90.29%). The best growth of plantlets shown in previous experiments immersed desiccant PEG 2.5% for 9 hours, and cultured in basic media with 2 g L-1 of activated charcoal.Keywords: desiccant, embryogenic callus, maturation, PEG 8000, somatic embryo ABSTRAK Pemuliaan tanaman melalui teknik in vitro efektif bila metode regenerasi eksplan optimum telah diperoleh. Rendahnya frekuensi perkecambahan dan tingginya abnormalitas, menjadi kendala pada embriogenesis somatik jeruk. Penelitian terdiri atas 3 percobaan paralel, bertujuan mengoptimalkan metode embriogenesis somatik jeruk, khususnya Keprok Batu 55. Percobaan pertama pematangan kalus embriogenik menggunakan Rancangan Acak Lengkap (RAL) satu faktor, dengan perlakuan penambahan ZPT (kontrol, 3 mg L-1 BAP, dan 2.5 mg L-1 ABA) pada media dasar (MS modifikasi vitamin MW) diperkaya 500 mg L-1 ekstrak malt. Percobaan kedua desikasi embrio somatik (fase kotiledon) menggunakan RAL dua faktor. Faktor pertama konsentrasi poly-ethylene-glicol/PEG 8000 (0, 2.5, 5, 7.5 dan 10%), dan faktor kedua waktu perendaman (kontrol, 3, 6, dan 9 jam) pada larutan desikan (media dasar + PEG). Percobaan ketiga mempelajari pertumbuhan dan perkembangan planlet, menggunakan RAL dua faktor. Faktor pertama konsentrasi PEG planlet pada percobaan kedua, dan faktor kedua perbedaan media dasar (tanpa dan dengan arang aktif). Hasil percobaan menunjukkan penambahan 2.5 mg L-1 ABA menghasilkan maturasi embrio somatik terbaik. Desikasi 9 jam menghasilkan frekuensi perkecambahan 90.29%. Pertumbuhan terbaik ditunjukkan planlet yang pada percobaan sebelumnya direndam 9 jam desikan PEG 2.5%, dan dibesarkan pada media dasar dengan 2 g L-1 arang aktif.Kata kunci : desikan, embrio somatik, kalus embriogenik, PEG 8000, pematangan
ELIMINASI ONION YELLOW DWARF VIRUS MELALUI KULTUR MERISTEM TIP PADA BAWANG MERAH (ALLIUM ASCALONICUM L.) ,, Aqlima; Purwoko, Bambang S.; Hidayat, Sri Hendrastuti; Dinarti, Diny
Jurnal Hortikultura Indonesia Vol. 8 No. 1 (2017): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (308.098 KB) | DOI: 10.29244/jhi.8.1.22-30

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ABSTRACTMeristem tip culture is culture of isolated meristem with 1-2 leaf primordia on suitable medium. This method is generally used to obtain free virus plant. Optimation of plant growth regulators (PGRs) was done to accelerate explant growth without callus formation and to avoid somaclonal variation in meristem tip culture. The aims of this study were to achieve the best combination of PGR for meristem tip growth and to evaluate meristem tip culture potential for Onion yellow dwarf virus (OYDV) elimination in shallot. This study used combination of PGRs 0.25 mg L-1 (2-ip, BAP, GA3, kinetin) with or without 0.1 mg L-1 IAA and medium without PGR. This research consisted of two experiments conducted separately. In experiment I, cv. Bima Brebes was used and experiment II cv. Tiron was used. Each experiment was arranged in completely randomized block design with single factor (PGR combination) that has 8 combination levels and 3 replications. The result showed that medium without PGR was the most efficient for meristem tip growth. Primary shoot was growing without callus formation. RT-PCR analysis showed that all of the tested samples were still infected by OYDV. Meristem tip culture method did not eliminate OYDV in both cultivars.Keywords: Auxin, cytokinin, GA3, OYDV, RT-PCRABSTRAKKultur meristem tip merupakan kultur meristem yang diisolasi 1-2 primordia daun dan pada media yang sesuai. Metode ini umum digunakan untuk mendapatkan tanaman bebas virus. Optimasi terhadap zat pengatur tumbuh (ZPT) dilakukan untuk mempercepat pertumbuhan eksplan tanpa disertai pembentukan kalus untuk menghindari terjadinya variasi somaklonal pada kultur meristem tip. Penelitian ini bertujuan untuk mendapatkan kombinasi ZPT terbaik bagi pertumbuhan meristem tip dan untuk mengevaluasi potensi kultur meristem tip dalam mengeliminasi virus Onion yellow dwarf virus (OYDV) pada tanaman bawang merah. Penelitian ini menggunakan 0.25 mg L-1 (2-ip, BAP, GA3, kinetin) dengan penambahan atau tanpa 0.1 mg L-1 IAA serta media tanpa ZPT. Penelitian ini terdiri atas 2 percobaan terpisah. Percobaan 1 menggunakan cv. Bima Brebes dan Percobaan 2 menggunakan cv. Tiron. Masing-masing percobaan disusun berdasarkan rancangan kelompok lengkap teracak (RKLT) dengan 1 faktor, yaitu kombinasi ZPT yang terdiri atas 8 taraf kombinasi dan 3 ulangan. Hasil yang didapat menunjukkan bahwa media tanpa penambahan ZPTmerupakan media yang paling efisien untuk pertumbuhan tunas meristem tip. Tunas utama tumbuh tanpa disertai pembentukan kalus. Hasil analisis RT-PCR menunjukkan bahwa seluruh sampel yang dideteksi masih terinfeksi OYDV. Metode kultur meristem tip belum dapat mengeliminasi virus OYDV pada kedua kultivar bawang merah.Kata kunci: Auksin, GA3, OYDV, RT-PCR, sitokinin
PENGARUH ELEKTROTERAPI DAN TERMOTERAPI SECARA IN VITRO TERHADAP ELIMINASI ONION YELLOW DWARF VIRUS Nasution, Siti Shofiya; Dinarti, Diny; Hidayat, Sri Hendrastuti
Jurnal Fitopatologi Indonesia Vol 13 No 6 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.004 KB) | DOI: 10.14692/jfi.13.6.199

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Infection of Onion yellow dwarf virus (OYDV) are reported causing problems in garlic production. Planting virus-free bulbs might help reduce viral disease incidence in the field. This research was aimed to develop method for eliminating OYDV from garlic bulbs using combination of electrotherapy (0, 5, 10, 15, and 20 mA each for 10 minutes) and thermotherapy (23, 28, 33, 38°C each for 4 weeks). Two garlic cultivars, i.e. Sangga Sembalun and Lumbu Hijau were used as seed bulbs for OYDV elimination tests. Virus infection was confirmed using transcription-polymerase chain reaction (RT-PCR).  The result showed that thermotherapy at 33 °Cwas the best method to eliminate OYDV in garlic although the efficiency was not the same for all cultivars. The efficiency reached up to 60% for cv. Lumbu Hijau, whereas for cv. Sangga Sembalun only reached up to 40%. Electrotherapy alone or in combination with thermotherapy were not able to produce OYDV-free plantlets.
IMPROVING THE EFFECTIVITY OF UREA FERTILIZER IN SHALLOT BY USING UREASE AND NITRIFICATION INHIBITORS Sugiyanta, Sugiyanta; Tustiyani, Isna; Dinarti, Diny
Jurnal Ilmu Pertanian Indonesia Vol. 24 No. 4 (2019): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (252.742 KB) | DOI: 10.18343/jipi.24.4.289

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Nitrification inhibitors are used to decrease the rate of nitrification process so it can decreases the nitrate losses. The objective of this study was to investigate the improvement of urea effectiveness by using urease and nitrification inhibitors on shallot. The study was conducted at Blubuk Village, Tanjung, Brebes District, Central Java, Indonesia from December 2017 to April 2018. The experiment was arranged in a randomized block design with 8 treatments and 3 replications. The treatments were untreated group (P0), 100% dose of Urea without inhibitor (control) (P1), (3) 100% dose of Urea + Urease Inhibitor (P2), 100% dose of Urea + Nitrification Inhibitor (P3), 100% dose of Urea + Urease Inhibitor + Nitrification Inhibitor (P4), 80% dose of Urea + Urease Inhibitor (P5), 80% dose of Urea + Nitrification Inhibitor (P6), and 80% dose of Urea + Urease Inhibitor + Nitrification Inhibitor (P7). The results showed that 100% dose of Urea + Urease inhibitor, 80% dose of Urea + Urease inhibitor, 80% dose of Urea + Nitrification inhibitor, and 80% dose of Urea + Urease inhibitor + Nitrification inhibitor treatments significantly produced higher plants heights, number of leaves, and more number of tillers compared to control treatment (100% Urea without inhibitors), without affecting the yield and yield components.
INDUKSI UMBI MIKRO TANAMAN DAUN DEWA (GYNURA PSEUDOCHINA (LOUR.) DC) SECARA IN VITRO DENGAN PERLAKUAN SUKROSA DAN DAMINOZIDE Hartanto, Donny; Aziz, Sandra Ari?n; Dinarti, Diny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 2 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.491 KB) | DOI: 10.24831/jai.v38i2.1799

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<!-- /* Font Definitions */ @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin-top:0cm; margin-right:0cm; margin-bottom:10.0pt; margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:Calibri; mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman"; mso-ansi-language:IN; mso-no-proof:yes;} @page Section1 {size:612.0pt 792.0pt; margin:72.0pt 90.0pt 72.0pt 90.0pt; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> The effects of sucrose and daminozide on in-vitro microtuber formation were evaluated for producing microtubers to supply year round microtubers and to facilitate sterilized explants exchange regionally and internationally. Uninodal stem segment explants were cultured on agar solidi?  ed Murashige and Skoog medium supplemented with 3% sucrose, 0.5 mg L-16-benzylaminopurine (BAP), 0.1 mg L-1 α-naphthaleneacetic acid (NAA) for bud multiplication for 3 months. Three nodals stem segment which already  formed  three  perfect leaves were cultured on agar solidi?  ed MS medium, 3% sucrose and 1 mg L-1 NAA for two weeks to induce root formation (until ± minimum 60% of the explants rooted). The next step was the induction of three nodal stem segments for microtuber formation on agar solidi?  ed MS medium supplemented with 5 mg L-1 BAP and two levels of sucrose, four concentrations of daminozide for 10 weeks. Sucrose at 6% resulted in the signi?  cantly highest number of microtuber. The daminozide 41.29 mg L-1 stimulated tuberization at base of the stems and reduce number of microtuber formation in stolon. Keywords: daminozide, Gynura pseudochina (L.) DC, in vitro, microtuber, sucrose
INDUKSI TETRAPLOID TUNAS PUCUK JERUK SIAM SIMADU (CITRUS NOBILIS LOUR) MENGGUNAKAN KOLKISIN SECARA IN VITRO Yulianti, Fitri; Purwito, Agus; Husni, Ali; Dinarti, Diny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 1 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (488.427 KB) | DOI: 10.24831/jai.v43i1.9593

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Seedless fruit is one of the criteria (necessary) to improve the quality of Simadu tangerine. The most effective method to obtain seedless triploid cultivars is hybridisation between tetraploid and diploid parents. Simadu tangerine is a diploid plant. Tetraploid Simadu tangerine can be obtained with doubling chromosome using colchicines.The aim of this research was to obtain tetraploid Simadu tangerine shoot which would serve as parent to produced seedless Simadu tangerine. Shoot-tips of Simadu tangerine without leaves were treated with colchicines at four different concentrations (0, 0.1, 0.2, and 0.3%) for 3 hours. The results showed that the high concentration of 0.3% reduced survival rate. The colchicine treatments reduced growth of shoot-tip of Simadu Tangerine.The leaves of colchicines treated shoots were thicker than control. Leaves from control (0% colchicine) and 0.1% colchicine treated shoots had 8.67 and 18.25 chloroplast per pair of guard cells. Compared to those of control, leaves with 0.1% colchicine had lower stomatal density, and larger stomatal size. It appeared that 0.1% colchicine treatment resulted in tetraploid Simadu Tangerine Shoot.Keywords:chloroplasts, doubling chromosomes, stomatal size, stomatal density
PENGARUH IRADIASI SINAR GAMMA TERHADAP PERTUMBUHAN PROTOCORM LIKE BODIES ANGGREK DENDROBIUM LASIANTHERA (JJ. SMITH) SECARA IN VITRO Cahyo, Fitro Adi; Dinarti, Diny
Jurnal Hortikultura Indonesia Vol. 6 No. 3 (2015): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.083 KB) | DOI: 10.29244/jhi.6.3.177-186

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ABSTRACTThe objective of this research was to determine the effects of gamma irradiation on protocorm like bodies (PLBs) Dendrobium lasianthera and Lethal dose (LD) 30 and 50 of gamma irradiation. The irradiation was conducted at the Center of Technology Application of Isotops and Radiation, Nuclear Energy Agency (PATIR-BATAN) and culture at Tissue Culture Laboratory of IPB from February 2014 to July 2014. The treatments were arranged in a completely randomized design (CRD) with a single factor of gamma irradiation doses, include i,g. 0 Gy, 20 Gy, 40 Gy, 60 Gy, 80 Gy, and 100 Gy. Each dose of gamma irradiation treatment was repeated 5 times. There were 30 experimental units. Each experimental unit consisted of five culture bottles containing 4 individually planted PLBs Dendrobium lasianthera. The results of this research showed that the increasing doses of gamma irradiation significantly decreased the percentage of alive PLBs, PLBs germination percentage, number of leaves, number of roots, the percentage of rooted PLBs. Morphological changes among other wider and spiral leaves were observed in the treated plantlets. Lethal dose 30% (LD30) was at 19.7697 Gy and LD50 was at 67.3504 Gy.Keywords: Dendrobium lasianthera, gamma irradiation, in vitro, Lethal dose (LD), mutation ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh iradiasi gamma terhadap pertumbuhan protocorm like bodies (PLBs) anggrek Dendrobium lasianthera serta menentukan Lethal dose (LD) 30 dan 50 dari iradiasi sinar gamma. Proses iradiasi dilakukan di Pusat Aplikasi Teknologi Isotop dan Radiasi, Badan Tenaga Nuklir Nasional (PATIR-BATAN) Proses kultur dilakukan di Laboratorium Kultur Jaringan IPB. Penelitian dilakukan dari bulan Februari 2014 hingga Juli 2014. Penelitian disusun menggunakan rancangan acak lengkap (RAL) dengan faktor tunggal yaitu dosis iradiasi gamma meliputi 0 Gy, 20 Gy, 40 Gy, 60 Gy, 80 Gy, dan 100 Gy. Setiap dosis perlakuan iradiasi gamma diulang 5 kali, seluruhnya terdapat 30 satuan percobaan. Setiap satuan percobaan terdiri atas lima botol kultur yang masing-masing ditanam 4 PLBs anggrek Dendrobium lasianthera. Hasil penelitian ini menunjukkan bahwa peningkatan dosis iradiasi gamma nyata menurunkan persentase hidup PLBs, persentase PLBs berkecambah, jumlah daun, jumlah akar, dan persentase PLBs berakar. Perubahan planlet in vitro yang teramati antara lain daun melebar dan daun spiral. LD30 berada pada 19.7697 Gy dan LD50 pada 67.3504 Gy.Kata kunci: Dendrobium lasianthera, in vitro, iradiasi gamma, Lethal dose (LD), mutasi
KETAHANAN BEBERAPA KLON KENTANG (SOLANUM TUBEROSUM L.) TERHADAP ASAM FUSARAT DAN PENYAKIT BUSUK KERING UMBI Sari, Dewi Citra; Dinarti, Diny; Suwarno, Willy Bayuardi; Purwito, dan Agus
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 2 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (451.676 KB) | DOI: 10.24831/jai.v44i2.13488

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ABSTRACTFusaric acid produced by Fusarium spp. played a major role in potato dry rot development. Using fusaric acid as a selection agent may be useful to identify resistant clones. The aim of this experiment was to evaluate the morphological responses of 10 potato clones (Granola, Atlantic, Cipanas, DTO 28, DTO 33, Russet Burbank, IPB 1, CIP 801040, CIP 801045, dan CIP 801050) and their resistance level to fusaric acid and Fusarium solani. The research was conducted in Plant Breeding Laboratory and Tissue Culture Laboratory 3, Departement of Agronomy and Horticulture from April 2014-February 2015. The in vitro experiment was arranged in a randomized block design with 4 fusaric acid concentrations and 4 replications, while the F. solani infection experiment was arranged in a completely randomized design with 5 replications. The result showed that fusaric acid inhibits growth, reduce microtubers production, and caused planlets death. Correlation analysis between in vitro resistance to fusaric acid and F. solani infection on tuber showed positive and notable result. Accordingly, fusaric acid can be used to identify any clones resistant to F. solani.Keywords: Fusarium solani, phytotoxin, selection
PENGELOLAAN PEMANGKASAN JERUK KEPROK (CITRUS SP.) DI KEBUN BLAWAN, BONDOWOSO, JAWA TIMUR Yuliana, Cucun; Dinarti, Diny; Widodo, Winarso D
Buletin Agrohorti Vol. 5 No. 3 (2017): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (389.878 KB) | DOI: 10.29244/agrob.v5i3.16485

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Program penelitian dilakukan di Blawan Estate, Bondowoso, Jawa Timur selama tiga bulan mulai tanggal 13 Februari sampai 13 Mei 2012. Tujuan program penelitian ini adalah untuk meningkatkan keterampilan teknis dan manajerial. Program penelitian ini dilakukan dengan menggunakan metode langsung dan tidak langsung, dengan mengikuti seluruh praktik di lapangan, observasi lapangan perkebunan, dan diskusi dengan staf (metode langsung). Informasi yang dikumpulkan termasuk data primer dan data sekunder. Kriteria pemangkasan, kondisi tanaman, prestasi kerja, waktu pemangkasan, dan pertumbuhan tunas meningkat sebagai data primer. Pemangkasan bisa menurunkan intensitas dan tingkat keparahan antraknosa. Namun, pengelolaan pemangkasan jeruk tidak dapat diimplementasikan sebagai SOP (Standart Operating Prosedure).