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Protective Effect of Green Algae Ulva reticulata Against Pseudomonas aeruginosa in Drosophila Infection Model Nainu, Firzan; Asri, Rangga Meidianto; Djide, M. Natsir; Ahsan, Muhammad; Arfiansyah, Rudi; Sartini, Sartini; Alam, Gemini
HAYATI Journal of Biosciences Vol. 26 No. 4 (2019): October 2019
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (491.119 KB) | DOI: 10.4308/hjb.26.4.%x

Abstract

The emergence of antibiotic-resistant Pseudomonas aeruginosa is one of main health issues in global communities. To overcome such threat, the discovery of novel antibacterial agents is indispensable. This study aimed to evaluate the in vivo antipseudomonal activity of Ulva reticulata extract in Drosophila model of infection. Ethanolic extract of Ulva reticulata was prepared using maceration method and the extract was subsequently assessed for its in vivo antibacterial effect against P. aeruginosa using survival assay, bacterial load enumeration, and gene expression analysis in the wildtype Drosophila. Survival and bacterial load analysis were further performed in a similar fashion on the mutant flies devoid of component responsible in the activation of immune responses against P. aeruginosa. Decline in the survival of infected host accompanied by augmentation of bacterial proliferation was documented in the wildtype Drosophila upon infection with P. aeruginosa. These phenotypic events were further amplified in immune-deficient mutant Drosophila. Nevertheless, improvement of host survivorship and reduction of bacterial burden were demonstrated in both wildtype or immune-deficient mutant flies upon treatment with Ulva reticulata extract after bacterial challenge. Our data demonstrated in vivo antipseudomonal activity of Ulva reticulata extract and thus provide a valuable information about its future potential for health promotion.
PRODUKSI SENYAWA BAKTERIOSIN SECARA FERMENTASI MENGGUNAKAN ISOLAT BAL Enterococcus faecium DU55 DARI DANGKE Razak, Abd. Rahman; patong, Abd. Rauf; Harlim, Tjodi; Djide, M. Natsir; Haslia, Haslia; Mahdalia, Mahdalia
Indonesia Chimica Acta Volume 2 No 2 - December 2009
Publisher : Indonesia Chimica Acta

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Abstract

Dangke is one of traditional food from Enrekang Province Sulawesi Selatan which is made from buffalo milk and enzimatically processed using papain from papaya’s gland secretion. Research on this local product as source of lactic acid bacteria (LAB) has been done. Counted 30 LAB was successfully isolated and 3 of them were potentially to be able to yield antimicrobial compound. Enterococcus faecium DU55 is one of LAB isolate available to be applied for producing bacteriocin compound through fermentation. Optimum condition of fermentation was specified by determining the highest antimicrobial activity generated by filtrate of fermentation result. Antimicrobial activity examination was carried out through diffusion agar method by measuring inhibition zone growth of pathogen bacterium Salmonella typhimurium FNCC 0050. Research results indicate that maximum bacteriocin compound production by BAL isolate E. faecium DU55 was obtained at condition of optimum fermentation at 30 °C during 42 hour using M1 medium with the same composition to medium MRS (Man Rogosa and Sharpe).Keyword: dangke, LAB isolate, bacteriocin, antimicrobial activity
PRODUKSI SENYAWA BAKTERIOSIN SECARA FERMENTASI MENGGUNAKAN ISOLAT BAL Enterococcus faecium DU55 DARI DANGKE Razak, Abd. Rahman; patong, Abd. Rauf; Harlim, Tjodi; Djide, M. Natsir; Haslia, Haslia; Mahdalia, Mahdalia
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 2 No 2 - December 2009
Publisher : Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v2i2.977

Abstract

Dangke is one of traditional food from Enrekang Province Sulawesi Selatan which is made from buffalo milk and enzimatically processed using papain from papaya’s gland secretion. Research on this local product as source of lactic acid bacteria (LAB) has been done. Counted 30 LAB was successfully isolated and 3 of them were potentially to be able to yield antimicrobial compound. Enterococcus faecium DU55 is one of LAB isolate available to be applied for producing bacteriocin compound through fermentation. Optimum condition of fermentation was specified by determining the highest antimicrobial activity generated by filtrate of fermentation result. Antimicrobial activity examination was carried out through diffusion agar method by measuring inhibition zone growth of pathogen bacterium Salmonella typhimurium FNCC 0050. Research results indicate that maximum bacteriocin compound production by BAL isolate E. faecium DU55 was obtained at condition of optimum fermentation at 30 °C during 42 hour using M1 medium with the same composition to medium MRS (Man Rogosa and Sharpe).Keyword: dangke, LAB isolate, bacteriocin, antimicrobial activity
Isolasi Dan Identifikasi Bakteri Termofil Penghasil Amilase Dari Sumber Air Panas Lejja Sulawesi Selatan Arfah, Rugaiyah A.; Patong, Abd. Rauf; Ahmad, Ahyar; Djide, M. Natsir
Al-Kimia Vol 2, No 2 (2014): Desember
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.196 KB) | DOI: 10.24252/al-kimia.v2i2.1644

Abstract

A Research isolation and identification of bacteria termofil amylase from hot springs Lejja South Sulawesi has been done. This study aims to characterize the morphological, biochemical, genus and species of bacteria producing  the enzyme amylase. The method used in this study through the stages: 1) Skrening and isolation of bacteria by means of as much as 1.0 mL of sample dilution plated on Petri dishes containing agar medium, then incubated for  20-24 hours at 50 °C, colonies of bacteria growing and has a colony morphology different character each taken 1 ose then etched into the amylolytic selective medium then incubated for 20-24 hours at 40oC and 50oC. Colonies that grew on selective media is scratched quadrant amylolytic to obtain pure isolates. Pure bacterial isolates taken 1 ose then grown in selective medium for 48 h at 50° C, bacterial isolates were grown spilled iodine solution (2% I2 and 0.2% KI) when there is a clearing zone around the colony indicated as the enzyme-producing bacterial isolates termofil amylase; 2) termofil characterization of bacterial isolates in microscopy with Gram stain; 3) isolates selected biochemical tests performed according  to the method Bergeys Manual and Systematic of Bacteriology. Results of screening and isolation of 10 bacterial isolates obtained amylase through iodine test, selected 2 isolates, 1 isolate from water samples RSAII-1B and 1 isolates from water samples mixed sediment RSSII-4B, which has a diameter of clearing zone of 5.6 cm respectively and 5.15 cm; out such characterization results of gram stain microscopy showed that the 2 isolates including gram + and shaped bacillus, the colony morphology as observed macroscopically, microscopy and  biochemical test results  obtained  RSAII isolates and isolates RSSII-1B-4B is a Bacillus sp.
Isolasi dan Implementasi Protein Bioaktif Kepah (Atactodea striata) Sebagai Bahan Obat Antibakteri Hasan, Tahirah; Patong, Abd. Rauf; Wahab, Abd. Wahid; Djide, M. Natsir
Al-Kimia Vol 2, No 2 (2014): Desember
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (270.675 KB) | DOI: 10.24252/al-kimia.v2i2.1652

Abstract

This study aimed to 1) determine the degree of saturation of ammonium sulfate right to extract and purify the bioactive protein from shells (Atactodea striata), 2) determine the fraction of active protein from shells (Atactodea striata) as a potential antibacterial. In this study used the Lowry method for determine protein concentration and agar diffusion method for antibacterial activity. Extraction of shells Atactodea striata was conducted by making use of buffer solution (0,1 M Tris-HCl of pH 8.3, 2 M NaCl, 0.01 M CaCl2, 1 % β-mercaptoethanol, and  0.5 % Triton X-100). Purification of proteins by  precipitation using ammonium sulfate at saturation level 30 %, 50 %, 70 %, and 90 %. The results showed that the protein concentration of the crude   extract is 41.6354 mg/mL. At fractionation rate of 0-90% saturation showed the highest concentration of protein found in fractions with 70% saturation level is 56.4184 mg/mL. The testing of antibacterial activity against Staphylococcus aureus showed that crude extracts and protein fractions Atactodea striata is considered effective as an antibacterial. The highest bioactivity during 24-hour incubation in protein fractions obtained by ammonium sulfate saturation level of 50% is 25.17 mm. Whereas the lowest activity was obtained at 90% saturation level is 14.05 mm. Bioactivity against Escherichia coli after incubation for 24 hours has the highest activity in the protein fraction with 30% ammonium sulfate saturation is 15.12 mm. Whereas the lowest activity was showed at 70% saturation level is 10.30 mm. After the observation was continued for 48 hours on both test bacteria, which formed a clear area becomes cloudy. It shows that the crude extract and fractions of protein tend to be bacteriostatic against Staphylococcus aureus and Escherichia coli.
PENGARUH KONSENTRASI JAMUR DIPO TERHADAP HASIL FERMENTASI SEDIAAN KOMBUCHA DENGAN SUBSTRAT TEH HITAM Daeng Pine, A. Tenriugi; Rahman, Latifah; Djide, M. Natsir; Kadir, Syahruddin
Jurnal Farmasi UIN Alauddin Makassar Vol 1, No 1 (2013): Jurnal Farmasi
Publisher : Jurusan Farmasi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (406.828 KB) | DOI: 10.24252/.v1i1.2089

Abstract

The aims of this study to get the valid data about the suitable starter concentration so thaht we can get the good kombucha preparation. This research was done by organoleptic test (taste, odour, colour) pH value by using the Schott pH-meter, the sugar concentration reduced by reduction-oxidation titration method by using Luff Schoorl solution, and the total value of acetic acid becteria by using GYCA (Glucose Yeast Calcium carbonat Agar) medium to the black tea extract which was fermentatedwith the mushroom of kombucha tea starter used 5%w/­v, 10%w/­v, 15%w/­v, 5%v/­v, 10%v/­v, dan 15%v/­v during 7 days. The result of analysis showed that in 15%w/v concentration has pH value = 2,96; total value of aid bacteria = 5,4.104 colony/ml; organoleptic value = 24; total concentration of acid = 0,14%; the sugar concentration reduced = 2,75% was the very suitable to get the good kombucha preparation with black tea substrat
Ekstraksi Isoflavon Kedelai dan Penentuan Kadarnya Secara Ultra Fast Liquid Chromatography (UFLC) Sartini, .; Djide, M. Natsir; Permana, A. Dian; Ismail, .
Sainsmat : Jurnal Ilmiah Ilmu Pengetahuan Alam Vol 3, No 2 (2014): September
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/sainsmat3211202014

Abstract

Isoflavon merupakan komponen polifenol utama dalam kacang kedelai. Isoflavon kedelai utamanya dalam bentuk glikosida (Genistin dan Daidzin) dibanding bentuk aglikonnya (Genistein dan Daidzein). Tujuan penelitian ini adalah untuk mengetahui pengaruh metode ekstraksi terhadap kandungan isoflavonnya (Genistein, Daidzein, Genistin, Daidzin). Isoflavon kedelai diekstraksi dengan beberapa cara, yaitu: 1) kacang kedelai tanpa kulit ari diblender dengan air panas, 2). Kacang kedelai tanpa kulit ari diblender dengan etanol 70 % , 3) kacang kedelai utuh diekstraksi secara maserasi dengan aseton 70 %, dan 4) kacang kedelai utuh diekstraksi secara maserasi dengan metanol.. Perbandingan kacang kedelai dan cairan penyari 1:10. Ekstrak yang diperoleh dianalisis kadar isoflavonnya menggunakan kromatografi cair kecepatan tinggi. Hasil penelitian menunjukkan total isoflavon tertinggi (dihitung sebagai total genistein, daidzein, genistin, daidzin) ada pada kacang kedelai utuh yang diekstraksi dengan metanol.Kata Kunci : Metode Ekstraksi, Isoflavon, Kedelai (Glycine max L.), Ultra Fast Liquid Chromatography