Articles

10. Isolation and Identification of Lactic Acid Bacteria Genus Pediococcus from Sumatran Orangutan (Pongo abelii) faeces at Kandi Zoo and Kinantan Zoo West Sumatera Reimena, Resti; Erina, Erina; Darniati, Darniati; Fakhrurrazi, Fakhrurrazi; Darmawi, Darmawi; Budiman, Hamdani
Jurnal Medika Veterinaria Vol 11, No 1 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (327.441 KB) | DOI: 10.21157/j.med.vet..v11i1.3524

Abstract

This research has purposes to isolating and identifying the lactic acid bacteria genus Pediococcus in sumatran orangutan (Pongo abelii) feaces. Feaces are isolated from one orangutan at Kandi Zoo and two orangutans at Kinantan Zoo. The isolating of the lactic acid bacteria was done by using streak plate method with the MRSA (de Man Rogosa Sharpe Agar) specific media. Identification was done by the morphological observing, Gram stain, catalase test, oxidase test and biochemical test. The result showed that one isolate feaces from Kandi Zoo has cocci shaped with the isolate prediction approaches genus Pediococcus sp. The characteristic features of isolate are cocci shaped, Gram positive, tetrad cell structure, nonmotile, catalase negative, and oxidase negative. Based on these characteristics, it can be concluded that there is a lactic acid bacteria coloni which grouped into genus Pediococcus in male orangutan feaces from Kandi Zoo
ISOLASI CANDIDA SP. DAN ASPERGILUS SP. PADA TEMBOLOK (INGLUVIENS) AYAM RAS DAN AYAM BURAS DI PASAR PEUNAYONG, BANDA ACEH Erina, Erina; Roslizawaty, Roslizawaty; Wahyuli, Sri
Jurnal Agripet Vol 19, No 1 (2019): Volume 19, No. 1, April 2019
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v19i1.13162

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ABSTRAK.  Penelitian ini bertujuan mengisolasi Candida sp. dan Aspergillus sp. pada tembolok ayam ras dan buras. Sampel dalam penelitian ini adalah tembolok ayam ras dan buras masing-masing berjumlah 15 sampel yang diambil secara acak dari tempat pemotongan unggas Peunayong Banda Aceh. Isolasi Candida sp. dan Aspergillus sp. dilakukan sesuai dengan metode Thompson (1969). Sampel dicuci dengan aquades steril yang diberi antibiotik selanjutnya ditanamkan pada media Sabouraud?s Dextrose Agar (SDA) kemudian diinkubasikan pada suhu kamar selama 2-7 hari. Pengamatan morfologi Candida sp. dan Aspergillus sp. diamati secara makroskopis. Koloni yang diduga Candida sp. dan Aspergillus sp. diperiksa secara mikroskopis. Data yang diperoleh dianalisis secara deskriptif. Hasil pemeriksaan menunjukkan bahwa Candida sp. dapat diisolasi pada semua sampel (100%) tembolok ayam ras dan ayam buras. Aspergillus sp. dapat diisolasi pada 2 dari 15 (13,33%) sampel tembolok ayam ras dan 6 dari 15 (40%) sampel tembolok ayam buras. Kesimpulan penelitian ini adalah Candida sp. tidak ada perbedaan pada tembolok ayam ras dan ayam buras sedangkan Aspergillus sp. pada tembolok ayam buras lebih banyak dari pada ayam ras.  (Isolation of Candida sp and Aspergillus sp. from crops (Ingluviens) of broiler and indigenous chicken in Peunayoung market, Banda Aceh) ABSTRACT. This research aimed to isolate Candida sp. and Aspergillus sp. from crop of chicken race (broiler) and indigenous chicken. This research used crops of the chicken race (broiler) and indigenous chicken, each animal consists of 15 animals taken randomly from the poultry of slaughter house Peunayong Banda Aceh. Isolation of Aspergillus sp. was done based on Thompson method (1969). The samples were washed with sterile aquadest containing antibiotics before implanted on Sabouraud?s Dextrose Agar (SDA), then incubated at room temperature for 2-7 days. The plate was observed from Candida sp. and Aspergillus sp. colony macroscopically and microscopically. Data were analyzed descriptively. The result showed that Candida sp. Found in all samples (100%) and Aspergillus sp. Found in 2 out of 15 (13,33%) crops samples in chicken race (broiler) and 6 out of 15 (40%) crops in indigenous chicken. The conclusion is, candida was found in both chickens race (broiler) and domestic chicken, while aspergillus was found more in indigenous chicken than chickens race broiler.
DETEKSI ANTIBODI SERUM TERHADAP VIRUS AVIAN INFLUENZA PADA AYAM BURAS Darmawi, Darmawi; Manaf, Zakiyah Heryawati; Darniati, Darniati; Fakhrurrazi, Fakhrurrazi; Abrar, Mahdi; Erina, Erina
Jurnal Agripet Vol 12, No 1 (2012): Volume 12, No. 1, April 2012
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v12i1.283

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Detection on Serum Antibodies of Native Chickens to Avian influenza VirusABSTRACT.  An important approach of controlling against Avian Influenza should be determined to detect the antibody titres of bird flu caused by Influenza virus H5N1 in Indonesia. The aim of the present study was to detect the antibodies to Avian Influenza in serum of native chickens. This study utilized 123 serum samples collected from the axilaris vein (left or right) of native chickens. Antibody titres were examined using Hemaglutination Inhibition (HI). The result showed that indication of natural infection by Avian Influenza (H5N1) in native chickens, as shown that out of 123 serum samples, 16 (13,01%) were tested positive by HI, while only 10 (8,13%) were tested protective to Avian influenza infection. Based on the results we obtained, a conclusion that natural infection by Avian influenza virus stimulated variety level of formation antibody titres in native chickens.
INHIBITION OF SALMONELLA SP. GROWTH IN COMPARISON WITH ANTIBIOTICS AND PINEAPPLE (ANANAS COMOSUS) SQUEEZE Admi, Masda; Erina, Erina; Azhari, Azhari; Balqis, Ummu; Hambal, Muhammad; Darmawi, Darmawi
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The purpouse of this study to examine antibiotics inhibition growth of Salmonella sp. isolated from feces of broiler chickens to chloramphenicol, gentamicin, and tetracycline in coparison with pineapple squeeze. Swab  sample  from  broiler  chicken  cloaca  was  cultured  to  nutrient  broth  media  and incubated at 37°C temperature for 24 hours. Culture was compared with Mc Farland 3 standardization, and separated on surface of Muller Hinton Agar (MHA). Antibiotic sensitivity was tested by mean of  chloramphenicol, gentamisin, and tetrasiklin in coparison with pineapple squeeze. The antibiotic disks (Oxoid) were put on the surface of MHA media which had inoculated with Salmonella sp. bacteria. Antibiotic sensitivity was determined based on formation of inhibition zone in surrounding of antibiotic disc. The result showed that Salmonella sp. bacteria growth was inhibited in ? 21 mm and ? 18 mm, ? 7 mm and ? 9 mm against chloramphenicol, gentamisin, tetrasiklin, and pineapple squeeze respectively. This research concluded that the Salmonella sp. cultured in this manner were sensitive to chloramphenicol and gentamisin. The inhibition growth of Salmonella sp. caused by chloramphenicol and gentamicin was significantly higher in comparison with pineapple (Ananas comosus) squeeze, meanwhile the Salmonella sp. bacteria was resistance against tetracycline. Our result suggest that both antibiotics chloramphenicol and gentamicin were recommended for antibiotic therapy in Salmonella sp. infection.
ANTI-TRYPANOSOMA ACTIVITY OF ETHANOLIC EXTRACT OF NEEM LEAF (AZADIRACHTA INDICA) ON TRYPANOSOMA EVANSI IN RATS (RATTUS NORVEGICUS) Fahrimal, Yudha; Maghfirah, Siti; Rinidar, Rinidar; Azhar, Al; Asmilia, Nuzul; Erina, Erina
Jurnal Kedokteran Hewan Vol 11, No 1 (2017): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i1.5450

Abstract

The aim of this study was to determine the effect of neem leaf extract (Azadirachta indica) on parasitemia of rats infected with Trypanosoma evansi (T. evansi) Aceh local isolate. A total of 24 male rats aged three months were used in this study and randomly divided into six treatment groups equally. The negative control group (K0) without T. evansi infection and neem leaf extract, the positive control group (K1) was infected with T. evansi but no neem leaf extract given, group K2, K3, K4, and K5 were infected with 5x104 T. evansi and were given neem leaf extract after patent infection with dose of 50, 100, 400, and 800 mg/kg BW respectively. The extract was given orally for three consecutive days. On the fourth day, rat blood was drawn for parasitemia examination. The results showed that no T. evansi detected in rats in negative control group (K0), while parasitemia in group K1; K2; K3; K4; and K5 was 12,295 x106/mL; 10,495 x106/mL; 9,360 x106/mL; 5,080x106/mL; and 2,398x106/mL of blood, respectively. Percentage of inhibition of parasitemia in K2, K3, K4, and K5 reached 14.64, 23.78, 58.68, and 80.50%, respectively. Based on the result of the study, neem leaf extract of 800 mg/kg BW gave the highest reduction of parasitemia in rats infected with T. evansi.
ISOLASI Escherichia coli dan Salmonella sp PADA TELUR AYAM KAMPUNG YANG GAGAL MENETAS DI LABORATORIUM LAPANGAN PETERNAKAN UNIVERSITAS SYIAH KUALA Sari, Lia Permata; Erina, Erina; Darniati, Darniati
JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 3 (2017): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (490.701 KB) | DOI: 10.21157/jim vet..v1i3.3816

Abstract

Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri Escherichia coli dan Salmonella sp pada telur ayam kampung yang gagal menetas di Laboratorium Lapangan Peternakan Universitas Syiah Kuala. Sebanyak 20 butir telur ayam kampung yang gagal menetas diperiksa di Laboratorium Mikrobiologi Fakultas Kedokteran Hewan, Universitas Syiah Kuala Banda Aceh. Sampel diisolasi berdasarkan metode Carter yang dimodifikasikan. Pada media Brilliance E.coli/ Coliform Selective Agar koloni bakteri tumbuh berbentuk bulat berwarna ungu, pada pewarnan Gram bakteri bewarna pink berbentuk batang pendek. Pada media SSA koloni bakteri berbentuk bulat bewarna hitam dan pada pewarnaan Gram bakteri bewarna pink berbentuk batang panjang. Hasil uji IMViC dan gula-gula sesuai dengan karakteristik Escherichia coli dan Salmonella sp. Hasil penelitian dari 20 sampel telur ayam kampung yang diteliti 3 sampel positif terinfeksi Escherichia coli dan 12 sampel positif Salmonella sp. Maka dapat disimpulkan Escherichia coli dan Salmonella sp merupakan salah satu penyebabab kegagalan menetas pada telur ayam kampung yang gagal menetas di Laboratorium Lapangan Peternakan Universitas Syiah Kuala.Kata kunci : telur ayam kampung, gagal menetas, Escherichia coli, Salmonella spThis study aims to isolat and identify bacteria Escherichia coli and Salmonella sp from failed to hatch domestic chicken in Field Laboratory of Animal Husbandry Syiah Kuala University. Twenty unhatched domestic chicken eggs were examined in microbiology laboratorium of Veterinary Faculty of Syiah Kuala University, Banda Aceh. Modified Carter methode was used to isolate bacterial from the samples. On media Brilliance E.coli/ Coliform Selective Agar colonies bacterial were purple and circular, on Gram staining it formed pink color cocobacil shape. On media SSA the colonies were black and circular, on Gram staining it formed pink color bacil shape. The result of Biochemistry test this showed that 3 of 20 domestic chicken eggs were positive infected by Escherichia coli and 12 of 20 sample were infected by Salmonella sp. It can conclude that Escherichia coli and Salmonella were one of the causes of failed to hatch of domestic chickens  egg’s.Keywords : domestic chicken eggs, fail to hatch, Escherichia coli, Salmonella sp
SEROPREVALENSI AVIAN INFLUENZA H5N1 PADA UNGGAS DI KABUPATEN ACEH UTARA Darmawi, Darmawi; Darniati, Darniati; Dewi, Maryulia; Fakhrurrazi, Fakhrurrazi; Abrar, Mahdi; Erina, Erina
Jurnal Agripet Vol 13, No 2 (2013): Volume 13, No. 2, Oktober 2013
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v13i2.815

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Seroprevalence of avian influenza H5N1 in birds in north aceh districtABSTRACT. Avian influenza virus H5N1 infections are an important cause of diseases in humans and several animal species, including birds. The present study conducted to investigate the seroprevalence Avian Influenza H5N1 in native birds from 15 sub-districts of North Aceh.  This study utilized 1108 serum samples collected from the axilaris vein (left or right) of birds. The standard Hemaglutination Inhibition (HI) assay was conducted at Microbiology Laboratory Faculty of Veterinary Medicine of Syiah Kuala University to determined serum antibody possitive or negative reaction against Avian influenza H5N1. The result showed that seroprevalence Avian influenza H5N1 virus was 4,7 % in North Aceh District. There were nine sub-districts were tested positively by HI test. However, the serum collected from six sub-districts did not react (negative) against Avian influenza H5N1. Based on the results we obtained, a conclusion that natural infection by Avian influenza virus in native birds occured in part of North Aceh District.
ISOLASI DAN IDENTIFIKASI BAKTERI Pseudomonas sp PADA IKAN ASIN DI TEMPAT PELELANGAN IKAN LABUHANHAJI ACEH SELATAN RAHMADIAN, CUT ADE; ISMAIL, ISMAIL; ABRAR, MAHDI; ERINA, ERINA; RASTINA, RASTINA; FAHRIMAL, YUDHA
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 4 (2018): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (691.899 KB) | DOI: 10.21157/jim vet..v2i4.9041

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ABSTRAKPenelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri Pseudomonas sp pada ikan asin di TPI Labuhanhaji Aceh Selatan. Penelitian ini menggunakan 15 sampel ikan asin dari 3 pedagang. Setiap pedagang diambil sejumlah 5 macam ikan asin, diantaranya ikan asin tongkol, dencis, layur, kembung, dan ikan kepala batu lalu diperiksa di Laboratorium Mikrobiologi Fakultas Kedokteran Hewan, Universitas Syiah Kuala Banda Aceh. Ikan asin di stomacher lalu di swab steril dan dimasukkan ke media Nutrien Broth  dan diinkubasikan pada suhu 37oC selama 24 jam, selanjutnya dilakukan penanaman pada media Pseudomonas Agar Base lalu diinkubasikan kembali pada suhu 37o C selama 24 jam. Koloni bakteri yang tumbuh pada media PAB dilakukan pengamatan bentuk bakteri, pigmentasi, permukaan, pinggiran, elevasi dan aspek koloni. Pewarnaan Gram dilakukan untuk memastikan bakteri yang didapat adalah kelompok Gram negatif. Terakhir dilakukan uji IMVIC,uji biokimia Triple Sugar Iron Agar dan uji gula-gula. Hasil penelitian ini di analisis secara deskriptif. Hasil pemeriksaan dari 15 sampel ternyata di temukan adanya pertumbuhan koloni bakteri Pseudomonas sp pada media PAB dan hasil pewarnaan Gram menunjukan Gram negatif. Oleh karena itu dapat di simpulkan bahwa ditemukan  bakteri Pseudomonas sp pada ikan asin di TPI Labuhanhaji Aceh Selatan. ABSTRACTThis study was aimed to isolate and identify Pseudomonas sp bacteria in salted fish in Fishing Auction Station (TPI) in Labuhanhaji Aceh Selatan. This study used 15 salted fish as the samples from 3 different traders. From each trader taken a number of 5 kinds of salted fish,  including Euthynnus affinis, Sardina piichardus, Trichiurus lepturus, Rastrelliger, and Osmeridae then they were checked in Microbiology Laboratory Faculty of Veterinary Medicine, Syiah Kuala University Banda Aceh. The salted fishs were placed in stomacher continued by putting them in sterile swab then they were put into Nutrient Broth medium incubated at 37oC for 24 hours, then planted on medium Pseudomonas Agar Base followed by incubating them again at 37oC for another 24 hours. Coloni of bacteria that grow on PAB were conducted to observe its bacterial form, pigmentation, surfaces, edges and elevation and the aspects of the colony. Gram staining was underdone to ensure the bacteria are the Gram-negative group. In the last treatment, IMVIC, Triple Sugar Iron Agar biochemical and sugar test were conducted. The result of this research is analyzed descriptively. The Results of examining 15 samples showed that there are the growth of Pseudomonas sp on PAB media and Gram staining results showed Gram negative. Therefore, it can be concluded that the Pseudomonas sp bacteria on salted fish in TPI Labuhanhaji Aceh Selatan were found. 
UJI DAYA HAMBAT EKSTRAK BIJI BUAH PALA (Myristica fragrans Houtt) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli (TEST OF INHUBITORY POWER OF NUTMEG SEEDS (Myristica fragrans Houtt) EXTRCT ON GROWTH OF BACTERIA Escherichia coli) Syarifah, Raysa; Fakhrurrazi, Fakhrurrazi; Harris, Abdul; Sutriana, Amalia; Erina, Erina; Winaruddin, Winaruddin
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 3 (2018): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (408.16 KB) | DOI: 10.21157/jim vet..v2i3.8203

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ABSTRAKPenelitian ini bertujuan untuk mengetahui daya hambat ekstrak biji buah pala (Myristicafragrans Houtt) terhadap pertumbuhan bakteri Escherichia coli. Biji pala yang digunakan dalam penelitian ini diperoleh dari pasar tradisional Darussalam, Banda Aceh. Proses ekstrasi biji buah pala menggunakan pelarut Etanol 96% dengan tingkat konsentrasi yang berbeda yaitu 1%, 10%,20%.  Menggunakan kertas cakram kosong (Blank disk) yang telah dicelupkan ke dalam ekstrak biji buah pala masing-masing pengulangan dan antibiotik ampisilin yang diletakkan pada media MHA. Pengujian daya hambat dilakukan dengan metode difusi Kirby-Bauer menggunakan kertas cakram pada media Mueller hinton agar (MHA) dengan 5 perlakuan dan 3 pengulangan. Media agar diinkubasi pada suhu 36-37ºC selama 24 jam. Hasil penelitian ini menunjukkan bahwa bahwa ektsrak  biji  buah  pala  memiliki  daya  hambat  yang  lemah  terhadap  pertumbuhan  bakteri Escherichia coli.  Ekstrak biji buah pala pada konsentrasi 1% memiliki daya hambat sebesar 9,73 mm, konsentrasi 10% sebesar 8,5 mm, konsentrasi 20% sebesar 8 mm dan kontrol positif sebesar20,83 mm.Kata kunci : antibakteri, ekstrak biji buah pala, Escherichia coli.. ABSTRACTThis study aims to determine the inhibitory power of nutmeg extract (Myristica fragransHoutt) on the growth of Escherichia coli bacteria. The nutmeg seed used in this study was obtained from the traditional market of Darussalam, Banda Aceh. Extraction process of nutmeg seed using96% ethanol solvent with different concentration level that is 1%, 10%, 20%. Using blank disc paper (Blank disk) which has been dipped into nutmeg extract of each repeating and ampicillin antibiotic placed on MHA media. Inhibitory testing was performed by the Kirby-Bauer diffusion method using paper discs on Mueller hinton agar medium (MHA) with 5 treatments and 3 repetitions. Media to be incubated at 36-37ºC for 24 hours. The results of this study indicate that the nutmeg nut extraction has a weak inhibitory effect on the growth of Escherichia coli bacteria. The extract  of nutmeg  seed  at concentration  of 1%  has inhibitory power  of 9.73  mm,  10%concentration of 8.5 mm, 20% concentration of 8 mm and positive control of 20.83 mm.Keywords : antibacterial, nutmeg ekstact, Escherichia coli.
ISOLASI DAN IDENTIFIKASI Escherichia coli DAN Staphylococcus aureus PADA KEUMAMAH DI PASAR TRADISIONAL LAMBARO, ACEH BESAR Ummamie, Liza; Rastina, Rastina; Erina, Erina
JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 3 (2017): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (688.407 KB) | DOI: 10.21157/jim vet..v1i3.4073

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Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri Escherichia coli dan Staphylococcus aureus pada keumamah yang dijual di pasar tradisional Lambaro, Aceh Besar. Penelitian ini menggunakan 10 sampel keumamah yang di jual di pasar tradisional Lambaro, Aceh Besar. Isolasi Escherichia coli dan Staphylococcus aureus berdasarkan metode Carter. Data hasil penelitian ini dianalisis secara deskriptif, berdasarkan keberadaan Escherichia coli dan Staphylococcus aureus yang terdapat pada Keumamah. Hasil penelitian ini menunjukkan bahwa satu sampel positif tercemar Escherichia coli, sedangkan sembilan sampel negatif  sementara pada Staphylococcus aureus didapat hasil positif untuk semua keumamah yang di jual di pasar tradisional Lambaro, Aceh Besar. Disimpulkan bahwa keumamah yang dijual di pasar tradisional Lambaro, Aceh Besar telah tercemar Escherichia coli dan Staphylococcus aureus.This study aimed to isolate and identify Escherichia coli and Staphylococcus aureus on keumamah sold at Lambaro traditional market,  Aceh Besar. Ten keumamah were used for sample. Escherichia coli and Staphylococcus aureus isolate based on the Carter methode. The result of this study were analyzed descriptively,  based on the presence of Escherichia coli and Staphylococcus aureus in keumamah.  The result showed that one sample is positive contaminated with Escherichia coli, and nine samples are negative. However for Staphylococcus aureus all those ten samples of keumamah  sold at Lambaro traditional market Aceh Besar are positively contaminated. It can be concluded that keumamah sold at Lambaro traditional market,  Aceh Besar were contaminated by Escherichia coli (10%) and Staphylococcus aureus (100%).
Co-Authors Abdul Haris Abdul Harris Abral, Mahdi Adhayani, Layli Admi, Masda Ahmad, Sagasias AK, M. Daud Al Azhar ALFURQAN, M.ARIF Amalia Sutriana Ananda Nasution, Annisah putri Ananda Putri Lestari, Ananda Putri ARDIANSYAH ARDIANSYAH Arman Sayuti aroza, muhammad Azhar Azhar Azhari Azhari Azmansyah, Azmansyah Basri Gani Bengi, Windian Tajuk Masmah Darmawi Darmawi Darniati Darniati Daud AK, M. Dewi, Karunita Eliawardani Eliawardani, Eliawardani Elsavira, Radhika Elvioleta, Iccha Erwin Erwin Fahkrurrazi, Fahkrurrazi Faisal Jamin Fakhrurrazi Fakhrurrazi Fema Amzani Nasution, Feby ferasyi, t reza Ginting, Septian Tri Mulyana Girsang, Florentina Magdalena Hadi, Devy Kartika Hamdani Budiman Harahap, Abdullah Azmi hayani, nisma Helmi, T. Zahrial Helmi, T. Zahrial Hendri Saputra HENNIVANDA, HENNIVANDA Indri Wahyuni isa, m Ismail Ismail Jalalluddin, M Jalaluddin, M Joni, Liza Setia Keliat, Silvia P.N M. Isa M. Nur Salim Maghfirah, Siti Mahdi Abrar Maryulia Dewi Matondang, Siti Wardianah Maulana, Ichsan Muhammad Hambal Muhammad Jalaluddin, Muhammad Muttaqien Bakri, Muttaqien N Siregar, Tongku NURLIANA NURLIANA Nuzul Asmilia Oktavia, Wahyuni poleh, Habibi sahidan Putri, Amelia Andika RAHMADIAN, CUT ADE Rapi, Dara Hayati Rasmaidar Rasmaidar Rastina, Rastina Razali Daud Reimena, Resti Rinidar Rinidar, Rinidar Roslizawaty Roslizawaty, Roslizawaty Rusli Rusli Safika, Safika Safika, Safika Salim, Muhammad Nur Sari, Lia Permata Sari, Nelma Savitri, Elza Sayuthi, Arman Siregar, Chairul Saputra siregar, rizki nurliyanti Sugito Sugito Suryandari, Latifa Susan Neni Triani, Susan Neni Suyoto, Balqis Alrasyid Syabaniar, Lisa Syarifah, Raysa T Armansyah, T T. Armansyah T. Fadrial Karmil Teuku Reza Ferasyi Teuku Zahrial Helmi Triva Murtina Lubis ulfizar, aulia Ummamie, Liza Ummu Balqis Wahyuli, Sri Winaruddin Winaruddin Yudha Fahrimal Zakiyah Heryawati Manaf Zuraidawati Zuraidawati, Zuraidawati