Found 7 Documents

Pre-treatment and Suitable Reagent Enabled a Reliable and Consistent for Molecular Detection of Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc4) Karmawan, Listya Utami; Dwivany, Fenny Martha; Esyanti, Rizkita Rachmi; Aryantha, I Nyoman Pugeg
HAYATI Journal of Biosciences Vol. 26 No. 4 (2019): October 2019
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (900.193 KB) | DOI: 10.4308/hjb.26.4.%x


Fusarium wilt which is caused by the soil-borne fungus, Fusarium oxysporum f.sp. cubense (Foc), is one of important diseases in banana plant. Foc tropical race 4 (Foc4) is the most pathogenic race which could infect various banana cultivars including Cavendish cultivar which was previously considered as resistant cultivar. Molecular detection of Foc using PCR analysis is indispensable to determine the race of Foc. We demonstrate a faster DNA isolation procedure described in previous method by substituting sodium acetate precipitation with ammonium acetate precipitation without affecting the result. Based on our experience, some fungal genomes were troublesome to be amplified. We suggested pre-treatment step prior to amplification procedure by incubating fungal DNA in 65°C for 10 minutes for any samples of fungal genome, including stubborn samples, before mixing into PCR mix reagent. PCR reagents should be tested for stubborn samples since some of the reagents were unable to amplify the desired DNA fragment. Pre-treatment and the choice of robust PCR reagent should be taken into consideration for a reliable and consistent Foc4 molecular detection result.
VIRULENCE STATUS OF Phytophthora infestans (Mont.) de Bary IN WEST JAVA TO POTATO PLANTS THAT CONTAIN RB GENE Susanti, Hani; Esyanti, Rizkita Rachmi; Sofiari, Eri
Widyariset Vol 16, No 2 (2013): Widyariset
Publisher : LIPI-Press

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Phytophthora infestans (Mont.) de Bary causes late blight (LB) disease which can decrease potato (Solanum tuberosum L.) production up to 100%. RB gene as source of resistance to LB could be obtained from genetically modified potato cultivars with resistance genes from wild species Solanum bulbocastanum. Evaluation virulences of P. infestans to potato contain RB gene is an important step to know effectiveness of controlling this disease. The research was focussed on determining virulence status of the isolates to the transgenic and hybrid potato plants containing RB genes. Isolates were collected from Bandung, Cianjur, and Garut, were cultured on V8-agar medium at 18 ± 20C. Virulence test were performed using “detached leaves assay” method. Based on statistic analysis, the average leaf infected by LB in potatoes containing the RB gene was significantly lower than potatoes in control group. Results showed that 40,8% transgenic leaves and 54,5% hybrid leaves which containing RB gene attacked by LB , whereas the control group was infected 98,7%. The virulence level of P. infestans isolates from West Java on potato plants contain RB gene is still low compare to that in the control group.
Pengaruh Elisitasi dengan Verticillium dahliae Kleb dan Rhizoctonia Solani Kuhn terhadap kandungan Gosipol dalam kalus Gossypium hirsutum L pada beberapa tingkat Subkultur Rahayu, Suci; Esyanti, Rizkita Rachmi; Siregar, Arbayah H
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 1 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.1.140


An experiment on gossypol content hasbeen conducted on callus derived from Gossypium hirsutum L. subcultures 5, 6, and 7 after elicitation with Verticillium dahliae Kleb and Rhizoctonia solani Kuhn. Callus was cultured on solid Linsmaier and Skoog  (LS)  medium  supplemented  with 10 –5 M NAA and 10 –6 M 2,4-D, and subcultured for 5, 6, and 7 times. Callus was then elicited with 40  μg dry weight /ml homogenate of fungi derived from V. dahliae and R. solani, and harvested 0, 2, 4, and 6 days after elicitation. The gossypol was analyzed qualitatively and quantitatively using High Performance Liquid Chromatography (HPLC). The maximum gossypol content elicited with R. solani was obtained on callus subculture 5, which was harvested on 4 days after elicitation, i.e. 177,995 ±  0,248 μg/g dry weight,  whilst that with V. dahliae  was obtained on subculture 5, harvested four days after elicitation, i.e.108,021± 0,507 μg/g dry weight. The gossypol content of control callus on subculture 5, 6, and 7 was 37,885 ± 0,779 μg/g dry weight  23,170 ±  0,003 μg/g dry weight, 12,284 ± 0,221 μg/g dry weight, respectively. The subculture level, elicitor type, and harvesting time gave significant effect on gossypol content of G. hirsutum callus culture.
PENGARUH KERAPATAN AGROBACTERIUM TERHADAP EFISIENSI TRANSFORMASI PADA PISANG CAVENDISH (Musa acuminata colla) Apriyani, Ria Khoirunnisa; Esyanti, Rizkita Rachmi; Dwivany, Fenny Martha
BIOTIKA Jurnal Ilmiah Biologi Vol 17, No 1 (2019): VOL 17, NO 1 (2019): BIOTIKA JUNI 2019
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v17i1.21898


Transformasi genetik melalui perantara Agrobacterium tumefaciens merupakan salah satu carauntuk meningkatkan kualitas buah Pisang Cavendish (Musa acuminata colla (AAA Group))yang memiliki susunan gen triploid. Efisiensi transformasi melalui A. tumefaciens terhadapembrio Pisang Cavendish dilakukan dengan mengoptimasi berbagai kerapatan optik kulturbakteri. A. tumefaciens, yaitu strain AGL1 dan GV3101 (yang masing-masing membawaplasmid ganda pBI121), mengandung gen gusA (pengode enzim β-Glucuronidase) digunakandalam proses transformasi ini. Perbedaan kerapatan optik (0,6; 0,8; 1,0) suspensi bakteri digunakan untuk proses transformasi. Persentase efisiensi transformasi dihitung berdasarkanjumlah embrio pisang yang positif pada uji GUS dibagi dengan jumlah total embrio pisang yangdiuji, dikali 100%. Efisiensi transformasi tertinggi diperoleh dari embrio pisang yangdiinokulasi oleh A.tumefaciens strain AGL1/pBI121 pada OD600nm = 1 (96%) dan efisiensitransformasi terendah diperoleh dari embrio pisang yang diinokulasi oleh A.tumefaciens strainGV3101/pBI121 pada OD600nm = 0.6 (40%).
Production Pattern of Ajmalicine in Catharanthus roseus (L.) G. Don. Cell Aggregates Culture in the Airlift Bioreactor ESYANTI, RIZKITA RACHMI; MUSPIAH, AIDA
HAYATI Journal of Biosciences Vol. 13 No. 4 (2006): December 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.025 KB) | DOI: 10.4308/hjb.13.4.161


A research has been conducted to optimize the rate of aeration and initial weight of cell aggregates in the production of ajmalicine in Catharanthus roseus cell culture in airlift bioreactor. Catharanthus roseus culture were grown in Zenk medium with the addition of 2.50 x 10-6 M naphthalene acetic acid (NAA) and 10-5 M benzyl amino purine (BAP). Cell aggregates were sub-cultured two times before transferring 20 and 30 g/fw of cell aggregates into bioreactor, respectively, and aerated with the rate of 0.25 l min-1 and 0.34 l min-1, respectively. The pattern of ajmalicine production in bioreactor were observed in every three days within 24 days. Qualitative and quantitative analysis were conducted using HPLC connected to Cromatopac CL-7A Plus. The results showed that the cell aggregates and medium contain ajmalicine. The highest concentration was obtained in combination of 30 g/fw and 0.34 l min-1 aeration compare to 20 g/fw - 0.25 l min-1, 20 g/fw - 0.34 l min-1, as well as 30 g/fw – 0.25 l min-1/sup>. The highest ajmalicine content in cell aggregates was obtained on the 12 days (79.23 µg g-) whilst in medium was obtained in the 18thsup> days (981.15 µg l-). Key words: ajmalicine, cell aggregates culture, C. roseus, airlift bioreactor
ANTAGONISITAS DAN EFEKTIVITAS Trichoderma sp DALAM MENEKAN PERKEMBANGAN Phytophthora palmivora PADA BUAH KAKAO Umrah, Umrah; Anggraini, Tjandra; Esyanti, Rizkita Rachmi; Aryantha, I Nyoman P.
Agroland: Jurnal Ilmu-ilmu Pertanian Vol 16, No 1 (2009)
Publisher : Universitas Tadulako

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The purpose of the study was to select several Trichoderma sp based on its sporalation capabilities, and antagonistic character, and effectiveness to control P. palmivora on cocoa pod.  The study consisted of two stages. In the first stage, a Completely Randomised design was used which consisted of seven treatments with three replicates: T. viridae, collection of  Laboratory of Plant Pest and Diseases of Bogor Institute of Agriculture (T-A), T harzianum, collection of Laboratory of Plant Pest and Diseases of Bogor Institute of Agriculture (T-B), T harzianum,  collection of  Coffee and Cacao Research centre of Jember (T-C), T koningii, collection of  Coffee and Cacao Research Centre of Jember (T-D), T. Trichoderma sp 1, collection of Laboratory of Microbiology  Life Sciences Centre of Bandung Institute of Technology    (T-E), Trichoderma sp 2, collection of Laboratory of Microbiology  Life Sciences Centre of Bandung Institute of Technology (T-F) and Trichoderma sp, collection of Laboratory of Plant Pest and Diseases University of Tadulako (T-G). Parameters measured were sporulation capability of Trichoderma sp, percentage of antagonisticity of Trichoderma sp towards in vitro P. palmivora . The second stage of the study was to determine the effectiveness of Trichoderma antagonisticity, either individual or mixed cultures in controlling the development of  P. palmivora on cocoa pod. The study was designed with seven treatments with three replicates:  T-D, T-E, T-G, T-DE  (mixed culture of T-D and T-E),  T-DG  (mixed culture of T-D and T-G), T-EG ( mixed culture of T-E and T-G), T-DEG (mixed culture of  T-D, T-E, and T-G). The results of the study showed that at in vitro condition the kinds of Trichoderma sp which had the highest antagonisticity on P. palmivora were  Trichoderma sp (T-G, T-E and T-D). They were effective to control P. palmivora on cocoo pod either in individual or mixed cultures.  However, T-G will be further developed due to its highest effectiveness as a biological control compared to the others.
CHITOSAN SUPPRESSES THE EXPRESSION LEVEL OF WRKY17 ON RED CHILI PLANT (CAPSICUM ANNUUM) UNDER DROUGHT STRESS Aziz, Muhammad Abdul; Esyanti, Rizkita Rachmi; Meitha, Karlia; Dwivany, Fenny Martha; Chotimah, Hany Husnul
Indonesian Journal of Biotechnology Vol 25, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.55016


Chili pepper plays a significant role in the global market. However, the production is often impeded by drought stress involving WRKYs gene as the defense regulator. Chitosan is considered as a promising alternative fertilizer and defense elicitor. Hence, this study aimed to determine the role of chitosan in improving the growth and resistance of red chili plants under drought stress. At the onset of the generative phase, chili plant was subjected to 1 mg mL-1 chitosan, 50% drought, or chitosan-drought treatment. Observations were made on various growth parameters, opened stomata, and WRKYs gene expression. The results showed that chitosan-drought treatment decreased plant growth and yield significantly. The percentage of opened stomata was recorded at 0.56 fold-lower than control. It was followed by the decrease of the relative expression of WRKY17 and WRKY53 genes up to 0.56 and 0.72 fold-lower than control, respectively. Therefore, we suggested that the double treatment of chitosan-drought might decrease plant growth performance but increase plant defense system through suppressing the expression level of WRKY17 gene.