Articles

EFFECT OF WHEY GOAT MILK KEFIR ON HYDROPHOBICITY OF E. COLI O157:H7, S. TYPHI BACTERIA AND C. ALBICANS Fardiaz, Dedi; Radiati, Lilik Eka
Jurnal Ilmu dan Teknologi Hasil Ternak (JITEK) Vol 7, No 1 (2012)
Publisher : Jurnal Ilmu dan Teknologi Hasil Ternak (JITEK)

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Abstract

The hydrophobicity of bacteria. was determined using BATH (Bacteria adhesion to hydrocarbon) test. All bacteria showed that 0,9 ml n-octane exposure gave a positive response and indicating that E. coli O157:H7 was categorized as moderate hydrophobic bacteria,  while S.  typhi  and C. albicans were catagorized as  highly hydrophobic bacteria. Goat Milk Kefir increased hydrophobicity of E.  coli O157:H7 by 24.40, however, decreased hydrophobicity of S. typhi by 47.56  and C. albicans by 70.14 percent, respectively. This finding showed that one of the inhibition mechanism may be caused by  an interaction  of  organic acid and peptide  compounds with cell membrane, in which hydrophobic sites of component  modified the hydrophobicity of the bacteria cell surface. The hydrophobicity modification in bacterial  cell wall might result inhibition of adhetion bacteria at cell host. Key words : Enterophatogenic bacteria, hidrophobisitas bacteria
PHYTOCHEMICAL CONTENTS OF TORBANGUN (COLEUS AMBOINICUS LOUR) FROM FRACTIONATION OF PRESSURIZED LIQUID EXTRACTION Laila, Farida; Fardiaz, Dedi; Yuliana, Nancy Dewi; Damanik, Muhammad Rizal Martua; Dewi, Fitriya Nur Anissa
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 2 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (453.286 KB) | DOI: 10.18343/jipi.25.2.224

Abstract

Coleus amboinicus (Lour) (torbangun) has been used traditionally as a breast milk stimulant, flavoring agent in many cuisines, and reported to possess many pharmacological values. The aim of this study was to explore the utility of the Pressurized Liquid Extraction (PLE) technique to identify the phytochemical contents of torbangun. In this study, total phenolic content and flavonoid in torbangun were determined using spectrophotometric method. The GC-MS analysis was used to identify the chemical constituents of torbangun, which was sequentially extracted with a wide range of solvent or solvent mixture from nonpolar-moderately polar and finally polar solvent. Total phenolic and flavonoid contents in the methanol extract of torbangun were 42.17±2.96 mg GAE/g and 11.20±0.58 mg QE/g, respectively. The identified chemical constituents in torbangun were sugar, hydrocarbon, ketone, terpenes, phenolics, fatty acid, fatty alcohol, steroids, alkaloids, and others, whereas the dominant constituents were phenolic compound, alkane, and sugar. In conclusion, this study demonstrated the effectiveness and rapid extraction of the PLE technique. Many valuable compounds in torbangun were extracted using gradual composition of solvent and were able to identify certain compounds in different polarities of solvents that are important in functional food preparation, pharmaceutical, and metabolomics research. Keywords: bioactive, extract, nontoxic, plant, solvent
KARAKTERISTIK WARNA DAN AKTIVITAS ANTIOKSIDAN ANTOSIANIN UBI JALAR UNGU [COLOR CHARACTERISTICS AND ANTIOXIDANT ACTIVITY OF ANTHOCYANIN EXTRACT FROM PURPLE SWEET POTATO] Mahmudatussa?adah, Ai; Fardiaz, Dedi; Andarwulan, Nuri; Kusnandar, Feri
Jurnal Teknologi dan Industri Pangan Vol. 25 No. 2 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (807.952 KB) | DOI: 10.6066/jtip.2014.25.2.176

Abstract

Anthocyanin is a natural pigment with color varying from red, purple, blue to yellow. The stability of its anthocyanin color is affected by pH, temperature and light. Purple sweet potato is rich in anthocyanin, particularly a stable acylated anthocyanin. This research was conducted to study the effect of pH on color and antioxidative activity of anthocyanin extracted from purple sweet potatoes harvested from Cilembu-Sumedang, Banjaran-Bandung, and Pakembangan-Kuningan. The experiments applied a completely randomized design with two replicates analyzed triplo.The results showed that the total number of monomeric anthocyanin in purple sweet potato harvested from Cilembu (3.78±0.08 mg cyanidin-3-glucoside/g dry weight, dw) was higher than that of Banjaran (3.18±0.01 mg/g) and Pakembangan (2.25±0.01 mg/g). The color of purple sweet potato anthocyanin extract was pH dependent. The color changed from red, faded red, purple, blue, green and yellow along with the increase of pH from 1 to 14. The content of anthocyanins from three locations of purple sweet potatoes differed from each other (p<0.05). Radical scavenging activity and reducing power of purple sweet potato anthocyanins extract at pH 1 was higher than that at pH 4.5 and pH 7. 
AKTIVITAS ANTIOKSIDAN EKSTRAK DAN FRAKSI EKSTRAK BIJI ATUNG (PARINARIUM GLABERRIMUM HASSK.) [ANTIOXIDANT ACTIVITIES OF PARINARIUM GLABERRIMUM HASSK EXTRACTS AND THEIR FRACTIONS] Sarastani, Dewi; Soekarto, Soewarno T.; Muchtadi, Tien R.; Fardiaz, Dedi; Apriyantono, Anton
Jurnal Teknologi dan Industri Pangan Vol. 13 No. 2 (2002): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The seed of Parinarium glaberrimum Hassk was extracted with non polar solvent (hexane) and or polar solvent (ethanol).  Antioxidative activity of extracts was measured by rates of carotene bleaching in the coupled oxidation of linoleic acid and b-carotene.  Ethanolic-Hexanoic Extract (EHE) of the seed was found to possess the highest antioxidative activity. Furthermore, EHE was fractionated by silica column chromatography and eight major fractions were isolated  according to UV absorption.  Antioxidative activity of these fractions was evaluated in a b-carotene-linoleate system.  Fractions III , I , and II showed the major activity, but fractions I and II have the best value of relative capacity, so they were chosen for further identification.
PRODUCTION OF PEPTONE FROM WASTE BEER INDUSTRY USING PAPAIN FOR BACTERIAL GROWTH MEDIUM ., Rahman; Fardiaz, Dedi; Idiyanti, Tami
Jurnal Teknologi dan Industri Pangan Vol. 15 No. 2 (2004): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

An experiment was conducted to hydrolyze waste of beer industry using papain to produce peptone. Papain with activity of 691.5 units based on casein substrat was used in this experiment. Results showed that optimum conditions for hydrolysis processes were as follows : substrate concentration 3.2%, papain concentration 0.4%, temperature 60-70OC, pH 6.0, hydrolysis time 5 hours. With 5 liter fermentation jar as much as 3.8 liter of hydrolyzate could be produced with 19.23% of peptone. The resulting peptone had the following characteristics : solubility 90.7%, N-amino 3.25%, N-total 11.23%, protein 70.19%, water 5.5% and ash 7.9%. This peptone gave the same effectivity for bacterial growth as that fron commercial Bacto peptone and Yeast extract to support the bacterial growth. Key words : Peptone, waste of beer industry, papain, bacterial growth medium.  
KARAKTERISTIK FUNGSIONAL PROTEIN MISELIUM JAMUR TIRAM MERAH MUDA DAN MERANG [FUNCTIONAL CHARACTERISTICS OF PROTEIN MYCELIUM OF PINK OYSTER AND PADDY STRAW MUSHROOMS] Sukarno, -; Hendartina, Nadia T.; Fardiaz, Dedi; Sukarno, Nampiah
Jurnal Teknologi dan Industri Pangan Vol. 25 No. 1 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (490.906 KB) | DOI: 10.6066/jtip.2014.25.1.72

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KARAKTERISTIK FUNGSIONAL PROTEIN MISELIUM JAMUR TIRAM MERAH MUDA DAN MERANG [Functional Characteristics of Protein Mycelium of Pink Oyster and Paddy Straw Mushrooms]Sukarno1)*, Nadia T. Hendartina1), Dedi Fardiaz1) dan Nampiah Sukarno2)1) Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Bogor2) Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Bogor Diterima 12 Maret 2014 / Disetujui 13 Juni 2014ABSTRACT Mycelium of mushroom contained high protein, which determined its functional characteristics such as water holding capacity (WHC), oil holding capacity (OAC), emulsion stability, and gel formation. This study aimed to determine the protein functional properties of Pleurotus flabellatus and Volvariella volvacea mycelia. Information obtained can be used to increase utilization of the mycelia as source of food. Mycelia biomass were obtained by growing the fungal cultures in Potato Dextrose Broth (PDB) on shaker at 100-150 rpm. Mycelia were harvested three times at 7, 8, and 9-days after inoculation for measuring their protein contents by kjehdahl method. Functional properties of mycelium protein measured were WHC, OAC, emulsion stability, and gel formation by folding test method. Based on the analysis of protein content in dry weight basis, 8-day old P. flabellatus and V. volvacea mycelia produced the highest protein contents with the value were 31.72 and 19.98%, respectively. Further analysis of protein functional properties showed that P. flabellatus mycelium had 10.38% of WHC, 0.52 mL/g of OAC, 57.14% of emulsion stability and gel strength level with the valueof  2, whereas the V. volvacea mycelium had 15.89% of WHC, 0.80 mL/g of OAC, 48.69% of emulsion stability, and did not form a gel. Protein functional properties of P. flabellatus were better than that of V. volvacea mycelium in terms of protein content, emulsion stability, and gel formation. 
KARAKTERISTIK KOMPOSISI KIMIA RUMPUT LAUT MERAH (RHODOPHYCEA) EUCHEUMA SPINOSUM YANG DIBUDIDAYAKAN DARI PERAIRAN NUSA PENIDA, TAKALAR, DAN SUMENEP Diharmi, Andarini; Fardiaz, Dedi; Andarwulan, Nuri; Heruwati, ndang Sri
Berkala Perikanan Terubuk Vol 39, No 2 (2011): Juli 2011
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (725.77 KB) | DOI: 10.31258/terubuk.39.2.%p

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he objective of research was to get the chemical composition of red algal(E. spinosum) from different coastal of water that have the potensial as a sourceof carrageenan. The analysis was conducted to analyze the chemical content ofthe algal (E. spinosum) of three coastal water were moisture content, ash content,protein content, fat content, carbohydrate content and dietary fiber content. Theresults of analysis chemical composition of red algal from different waters hadshowed that content of moisture content 19.55-21.27% , ash content 18.55-18.95%, protein content 4.85-5.59% content, fat content of 0.06-0.1% andcarbohydrate content 53.44-55.52 % while the total dietary fiber content of redalgal (E. spinosum) was 12.78-15;92%. The chemical composition of red algal E.spinosum of three coastal water no real effect unless total dietary fiber contentwas significantly diffrence between the coastal water.
IDENTIFIKASI KOMPONEN KIMIA DAMAR MATA KUCING (SHOREA JAVANICA) DENGAN METODE PIROLISIS-GC/MS Mulyono, Noryawati; Wijaya, Christofora Hanny; Fardiaz, Dedi; Rahayu, Wuryaningsih Sri
Jurnal Natur Indonesia Vol 14, No 1 (2012)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (162.528 KB) | DOI: 10.31258/jnat.14.1.155-159

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The aim of this research was to identify chemical compounds in cat eye dammar. The method included functional groups characterizationby infrared spectrophotometer and identification using Pyrolisis-GC/MS. Infrared spectra of crude sample showed that there were somefunctional groups such as alkyl, carbonyl, vinyl, and hydroxyl. Identification by Pyrolisis-GC/MS showed that dammar consisted of at least67 compounds. This natural gum components could be categorized into 4 groups, i.e. tetra cyclic hydrocarbon (30 compounds, 49.57%),penta cyclic (3 compounds, 2.56%), C 15 compounds (11 compounds, 17.09%), and other group (23 compounds, 18.26%). According to thePy-GC/MS data, brassicasterol is the highest relative concentration in dammar, i.e. 20%.
KARAKTERISTIK NANOEMULSI MINYAK SAWIT MERAH YANG DIPERKAYA BETA KAROTEN YULIASARI, SHANNORA; FARDIAZ, DEDI; ANDARWULAN, NURI; YULIANI, SRI
853-8212
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

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Abstract

ABSTRAKMinyak sawit merah (Red palm oil/RPO) dan β-karoten tidak larutdalam air sehingga sulit diaplikasikan ke dalam produk pangan. Salah satupendekatan untuk meningkatkan kelarutan RPO dan β-karoten adalah emulsifikasi. Penelitian ini bertujuan untuk mendapatkan nanoemulsi RPOdiperkaya β-karoten yang stabil. Penelitian dilaksanakan di LaboratoriumSEAFAST CENTER IPB dari Januari–September 2013. Pada penelitiantahap pertama, nanoemulsi disiapkan melalui tahap-tahap: pengayaan RPOdengan β β-karotenmenggunakan HPH (High Pressure Homogenizer) pada tekanan 34,5 MPadengan 10 siklus. Rasio RPO dan air dalam emulsi adalah 5 : 95; 7,5 :92,5; dan 10 : 90 (b/b), dan persentase Tween 80 sebagai pengemulsiadalah 2,5; 5,0; 7,5; dan 10% (b/b) dari total emulsi. Pada tahap kedua,nanoemulsi disiapkan dengan persentase RPO: 2, 4, dan 6% (b/b) danpengemulsi 1,5; 3,0; dan 4,5% (b/b) dari total emulsi. Hasil penelitiantahap pertama menunjukkan nanoemulsi yang dibuat dengan rasio RPO :air = 5 : 95 dan 7,5 : 92,5 serta pengemulsi 5% (b/b) menghasilkan emulsidengan ukuran droplet 115,1 sampai 145,2 nm dan stabil. Nanoemulsiyang dihasilkan dari penelitian tahap kedua memiliki ukuran droplet 94,9sampai 125,5 nm, dan kadar β-karoten antara 47,6 sampai 130,9 mg/l.Ukuran droplet nanoemulsi yang kurang dari 125 nm dapat dihasilkandengan formula rasio RPO dan pengemulsi kurang dari 2,0.Kata kunci: minyak sawit merah, β-karoten, nanoemulsi, homogenizerABSTRACTRed palm oil (RPO) and β-carotene are insoluble in water. It makescan be used to improve RPO and βThis research is aimed to produce stable RPO nanoemulsion enriched withβ-carotene. The research was conducted in the SEAFAST CENTERLaboratory, Bogor Agriculture University from January to Septemberfollowing steps, i.e. enrichment of RPO with βusing a high pressure homogenizer at a pressure of 34.5 MPa in 10 cycles.The ratio of RPO and water in the mixture were 5 : 95; 7.5 : 92.5; and 10 :10% (w/w) of the total emulsions. In the second stage, nanoemulsionswere prepared on various RPO percentage of 2, 4, and 6% (w/w) andhad a droplet size from 115.1 to 145.2 nm and stable. Nanoemulsions wereresulting from the second stage had droplet size from 94.9 to 125.5 nm,and β-carotene content were 47.6 to 130.9 mg/l. Droplet size ofnanoemulsions is less than 125 nm. It can be produced with RPO andKey words: red palm oil, β-carotene, nanoemulsion, homogenizer
FRAKSINASI KERING MINYAK KELAPA MENGGUNAKAN KRISTALISATOR SKALA 120 KG UNTUK MENGHASILKAN FRAKSI MINYAK KAYA TRIASILGLISER0L RANTAI MENENGAH MURSALIN, MURSALIN; HARIYADI, PURWIYATNO; PURNOMO, EKO HARI; ANDARWULAN, NURI; FARDIAZ, DEDI
853-8212
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

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Abstract

ABSTRAKMinyak kelapa merupakan sumber medium chain triglycerides(MCT) utama. Melalui proses fraksinasi dapat dihasilkan fraksi minyakdengan kandungan MCT tinggi. Penelitian ini bertujuan untuk mempelajaripengaruh berbagai faktor perlakuan dingin terhadap kristalisasi danfraksinasi minyak kelapa, serta untuk menetapkan prosedur pendinginanyang efektif dalam menghasilkan fraksi minyak dengan kandungan MCTtinggi. Penelitian dilaksanakan di Laboratorium SEAFAST CENTER IPBdari bulan Maret 2012 sampai bulan Februari 2013. Fraksinasi dilakukandengan memanaskan minyak pada suhu 70°C lalu didinginkan padaberbagai laju pendinginan untuk mencapai beberapa variasi suhukristalisasi, diaduk dengan kecepatan 15 rpm, dibiarkan mengkristal padalama waktu yang berbeda (hingga 900 menit), serta difraksinasi denganpenyaringan vakum menggunakan kertas Whatman 40. Tiga tahappendinginan yang merupakan faktor kunci keberhasilan proses kristalisasiminyak kelapa yaitu tahap pendinginan awal dari suhu 70 hingga 29°C;tahap pendinginan kritis 29°C hingga suhu kristalisasi; dan tahapkristalisasi itu sendiri. Pada tahap pertama minyak kelapa didinginkansecepat mungkin untuk menurunkan waktu proses, tetapi pada tahap keduaharus dilaksanakan dengan laju pendinginan lambat (kurang dari 0,176°C/menit) untuk menghasilkan kristal yang berukuran besar dan tidak mudahmeleleh. Minyak dengan kandungan triasilgliserol tinggi dapat diperolehdari fraksi olein minyak kelapa. Pada perlakuan suhu kristalisasi 21,30-21,73°C untuk laju pendinginan kritis antara 0,013 hingga 0,176°C/menit,semakin rendah laju pendinginan kritis dan semakin lama proseskristalisasi maka kandungan MCT fraksi olein yang dihasilkan akansemakin tinggi.Kata kunci: minyak kelapa, laju pendinginan, kristalisasi, fraksinasi, MCTABSTRACTCoconut oil is the main source of medium chain triglycerides(MCT). Fractionation produce oil fraction containing MCT concentrate.This research aims to study the influence of various factors of coolingtreatment on the crystallization and fractionation of coconut oil, and toestablish effective cooling procedure to produce oil fraction with highMCT content. The research was conducted in Laboratorium of SEAFASTCENTER IPB from March 2012 to February 2013. Coconut oil washeated at 70°C then cooled at different cooling rate to reach variouscrystalization temperatures. The oil was then stirred at 15 rpm and allow tocrystallized at different period of time (up to 900 min), and finallyfractionated by vacuum filtration using Whatman #40 paper. Fractionationtemperatures was the same as crystalization temperature. The resultsshowed that there were three distinct cooling regimes critical tocrystallization process, i.e temperature range from 70 to 29°C; 29°C tocrystallization temperature; and crystallization temperature. In the firstregime, melted coconut oil might be cooled quickly to save time, but in thesecond regime need be done with a cooling rate of less than 0.176°C/minto produce physically stable crystal. Oil with high triacylglycerol contentcould be obtained from olein fraction of coconut oil. At the crystallizationtemperature 21.30-21.73°C for the critical cooling rate between 0.013 to0.176°C/min, the higher MCT content of olein fraction were produced bythe lower critical cooling rate and the longer crystallization process.Keywords: fractionation, crystallization, MCT, coconut oil, cooling rate.