Ratna Farida
Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430

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The Effects of Triethylene Glycol Dimethacrylate (TEGDMA) on the Protein of Human Dental Pulp Cells Farida, Ratna; Fatma, Dewi; Karina, Karina; Tanaya, Tanaya S.; Pardamean, Pardamean RAS
Journal of Dentistry Indonesia Vol 16, No 3 (2009): December
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3021.503 KB) | DOI: 10.14693/jdi.v16i3.102

Abstract

Triethylene glycol dimethacrylate (TEGDMA) is a common component of the bonding agents and resin composites used in dentistry for restorative dentistry. However, TEGDMA could be released from composite resins following incomplete polymerization and degradation processes by salivary enzyme in the mouth. Subsequently, TEGDMA is available in saliva and diffuses toward and affects the dental pulp which contains various cells, and thus may cause severe cytotoxic effects. Objectives: To determine the total protein concentration of human dental ulp cells following exposure to TEGDMA. Materials and Methods: Dental pulp cells were isolated from the pulp of the freshly extracted teeth and cultured in DMEM for 48 h (37ºC, 5% CO2). Then, 2 mM and 4 mM, and 8 mM TEGDMA were added to these cells and incubated for 24 h. The total protein was measured by Bradford Protein Assay. Results: The total protein concentration of dental pulp cells after exposure to 4 mM, 8mM, and 12 mM TEGDMA were statistically lower (22762.27 µg/ml ± 3385.87; 20268.44 µg/ml ± 1701.14; 23706.51 µg/ml ± 3214.52; respectively) than the control group (24253.77 µg/ml ± 3072.99). Furthermore, the total protein concentration of culture medium after exposure to 4 mM, 8 mM, and 2 mM TEGDMA, were statistically higher (28635.85 µg/ml ± 2373.4; 35288.41 µg/ml ± 3469.48; 38199.79 µg/ml ± 2752.47; respectively) when compared with the controls (27073.83 µg/ml ± 2772.47). Conclusion: 2 mM, 4 mM, and 8 mM TEGDMA caused cytotoxity to human dental pulp cells chowed by decreasing the total protein of cells and increasing the total protein of the culture medium.DOI: 10.14693/jdi.v16i3.102
REAKSI RADANG Farida, Ratna
Journal of Dentistry Indonesia Vol 10, No 2 (2003): AUGUST
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (365.925 KB) | DOI: 10.14693/jdi.v10i3.467

Abstract

Inflammatory respone is a non-specific response to an injurious stimulus in vascularized tissue. The purpose of this response is to destruct noxious agent, limitation of its spread, and preparation for reconstitution or repair of injured area. Inglammation is classified into acute and chronic types. The classical features of redness, swelling, warmth, pain and loss of function, all of which reflect the effects of cytokines and other inflammatory mediators on the local blood vessels in acute inflammation. The cellular response is mainly of neutrophil polymorphonuclear leukocytes. Furthermore, chronic mononuclear cells-macrophages, lymphocytes, and plasma cells. A proliferation of blood vessels also occurs. There is also associated collagen production by fibroblasts resulting fibrosis. Cytokines secreted by macrophages play prominent roles in inflammation. However, other soluble factors, such as vasoactive amines, lipid mediators, complement fragments C5a, C3a, and C4a, prostacyclin and nitric oxide are also major players in inflammation. The inflammatory reactions in the mouth and teeth are identical to inflammatory reactions anywhere else in the body. Such as pulpitis is similar to inflammation in other connective tissue anywhere in the body.
SERUM ANTIBODI-IMUNOGLOBULIN-G-SUBKLAS SPESIFIK TERHADAP NON-LEUKOTOKSIK ACTINOBACILLUS ACTINOMYCETEMCOMITANS Farida, Ratna
Journal of Dentistry Indonesia Vol 7, No 1 (2000)
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.653 KB) | DOI: 10.14693/jdi.v7i1.509

Abstract

Serum specific IgG subclass antibodies to non-leukotoxic strains of Actinobacillus actinomycetemcomitans (Aa) NCTC 9710 and NCTC 10979 were quantified by radioimmunoassay. Samples were taken from sera of 10 patients with Juvenile Periodontitis (JP) and 10 from healthy periodontal individuals as control. The results show that JP patients had higher IgG2 antibody levels to Aa NCTC 9710 (ABT50 = 620) than the other IgG subclasses (IgG1 = 300, IgG3 = 370, and IgG4= 500). The JP patients had also higher IgG2 antibody levels to Aa. NCTC 10979 (600) when compared with the other IgG subclasses (IgG1 = 300, IgG3 = 500, and IgG4= 400). Whilst, the control gorup had lower IgG subclass antibody level as compared with the JP group. In conclusion, the JP patients had an elevation of IgG2 subclass antibodies to non-leukotoxic Actinobacillus actinomycetemcomitans.
Evaluasi Tingkat Migrasi Neutrofil (OMR) dalam Mulut pada Mahasiswa FKG UI dengan Stres Akademik Suryadhana, Nyoman Gde; Utami, Sri; Joenoes, Hedijanti; Farida, Ratna; Yetty, Yetty S.
Journal of Dentistry Indonesia Vol 4, No 3 (1997): December
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4304.749 KB) | DOI: 10.14693/jdi.v4i3.754

Abstract

A study has been done on the relationship between anxiety and migration rate of PMN in the mouth. Subjects were dental students of University of Indonesia from the first and the third semester, consisting of 12 males and 97 females, 19-25 years old, and not suffering from any physical diseases or psychotic disorders. This study had a within-subject design with each student as his or her own control. Observation was done three times, about two months and half an hour before examintation and half an hour after examination. The result was that the migration rate of PMN 30 second (index of OMR) declines significantly just a moment before examination and recovers after examination (P<0.01). The OMR index was 0.43 well before examination, 0.23 shortly before it and 0.42 after examination. Apparently, the academic stress can temporarily inhibit some facet of the immune response showing a measurable abnormality of the index OMR. Results of this research could be useful for mental preparation of students taking examinations. Finally, this may give clue for the genesis of suggested stress related diseases which work through an immunological mechanism.
Status Karies Gigi Para Anggota di Lingkungan Mabes TNI-AL, Cilangkap Jakarta Tjahjani, Agoeng; Farida, Ratna; Cara, Cara M.; Utami, Sri; Djais, Ariadna
Journal of Dentistry Indonesia Vol 5, No 2 (1998): August
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1953.035 KB) | DOI: 10.14693/jdi.v5i2.770

Abstract

National survey of dental health in 1995 indicated a moderate incidence of the disease among all age groups. In order to increase the dental health status, a comparison of interest both military and civilian prevalence dengal caries was undueted. In 1996, a random sample of 258 officer, active duty, TNI-naval personnel were undertaken for monitoring the prevalence dental decay and severity of dental caries. Results show that the average DMF-T rate was 2.44 and 3.12 for both military and civilian personnel. The prevalence dental decay was 51.94% and had a significant at p < 0.001. The results show that dental health status of military personnel was better than civilian, besides the moderate incidence of dental caries.
Penentuan Golongan Darah Sistem ABO Melalui Saliva Mahasiswa Fakultas Kedokteran Gigi U.I. (Laporan Penelitian) Farida, Ratna; Suryadhana, Nyoman Gde; Gultom, Ferry
Journal of Dentistry Indonesia Vol 1, No 1 (1993)
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2879.901 KB) | DOI: 10.14693/jdi.v1i1.931

Abstract

Penentuan golongan darah sistem ABO sampai saat ini dilakukan dengan menggunakan darah individu yang kadang-kadang tidak disukai. Dengan berkembangnya ilmu kedokteran, maka penentuan golongan darah ini dapat juga dilakukan dengan menggunakan saliva, tetapi tidak semua individu dapat ditentukan golongan darahnya melalui saliva. Hanya ± 80% populasi kulit putih saja yang dapat digunakan salivanya untuk menentukan golongan darah. Populasi ini disebut golongan secretor, sedangkan yang 20% disebut golongan non secretor. Berdasarkan uraian tersebut dan mudahnya pengambilan saliva tanpa menyakiti individu, maka pada penelitian ini ingin diketahui populasi orang Indonesia yang bergolongan sekretor dengan mengambil sampel saliva dari mahasiswa Fakultas Kedokteran Gigi Universitas Indonesia sebagai tahap awal. Penentuan golongan darah melalui saliva pada penelitian ini dilakukan dengan menggunakan cara inhibition of agglutination test. Hasil penelitian ini menunjukkan bahwa 78.50% dari 214 mahasiswa adalah golongan sekretor. Hasil ini menunjang hasil penelitian sebelumnya bahwa ± 80% populasi adalah golongan sekretor. Penelitian ini juga melaporkan adanya variasi golongan sekretor antara pria (97%) dan wanita (74%). Variasi golongan sekretor masing-masing adalah golongan darah A (94%), B (60%), dan AB (87%).
PENGEMBANGAN MODEL PEMBELAJARAN FLIPPED CLASSROOM DENGAN TAKSONOMI BLOOM PADA MATA KULIAH SISTEM POLITIK INDONESIA Farida, Ratna; Alba, Amru; Kurniawan, Rudi; Zainuddin, Zamzami
Kwangsan: Jurnal Teknologi Pendidikan Articles in Press
Publisher : Balai Pengembangan Media Televisi (BPMTV) Kemdikbud

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Abstract

Traditional learning instruction puts students in a passive learning role such as listening to lectures in the classroom. One of the contemporary innovative instructional approaches is the "flipped classroom", which employs pre-class video-recorded lectures and critical in-class discussion. This instruction leads students to remember and understand the pre-class content or access videos before attending the class. In this study, we proposed the flipped learning model design in which relevant to the Indonesian Political Systems course. This preliminary study employed Research-Based Design as a methodological approach in collecting rigorous information from two experts in the field of educational technology and pedagogy. The study was conducted within several phases, namely: planning, designing, formative evaluation, revision, redesign, and summative evaluation. The finding of this study reveals that two activities are recommended to be applied for students before class (lower-order thinking skills)and the other four activities are practiced in the classroom (higher-order thinking skills). This study can be summarized that the employment of flipped-class instruction may potentially lead to independent learning and critical thinking skills. Besides, this study also becomes a recommendation for the Ministry of Research, Technology, and Higher Education (Ristekdikti) to consider the flipped classroom as a contemporary teaching-learning conception in Indonesia. AbstrakPembelajaran dengan metode konvensional seperti mendengarkan ceramah di kelas cenderung membuat peserta didik menjadi pasif dan akhirnya membuat kegiatan belajar-mengajar menjadi tidak menyenangkan dan membosankan. Salah satu model pembelajaran inovatif terbaru berbasis video pembelajaran dan diyakini dapat menjadi solusi untuk masalah ini adalah flipped classroom. Model pembelajaran ini akan menuntun peserta didik untuk belajar secara mandiri melalui video pembelajaran sebelum datang ke kelas, sedangkan kegiatan di kelas lebih difokuskan pada aktivitas diskusi dan tanya-jawab. Oleh karena demikian, penelitian ini bertujuan untuk mendesain model pembelajaran flipped classroom atau kelas terbalik untuk diterapkan pada sebuah perguruan tinggi dengan mata kuliah Sistem Politik Indonesia. Studi sederhana ini menggunakan pendekatan Desain Berbasis Penelitian (Design-Based Research). Penelitian ini dilakukan dalam beberapa tahap, yaitu: perencanaan, perancangan, evaluasi formatif, revisi, desain ulang, dan evaluasi sumatif oleh dua orang pakar. Hasil dari penelitian ini menunjukkan bahwa model pembelajaran flipped classroom bisa diimplementasikan dalam enam tahapan kegiatan; dua kegiatan sebelum datang ke kelas (lower-order thinking skills) dan empat kegiatan di dalam kelas (higher-order thinking skills). Dari hasil penelitian ini, dapat disimpulkan bahwa model pembelajaran inovatif flipped classroom berpotensi untuk diterapkan dalam kegiatan belajar-mengajar di perguruan tinggi, bertujuan untuk membangun budaya belajar mandiri dan berfikir kritis mahasiswa. Studi ini juga diharapkan bisa menjadi masukan bagi Kementerian Riset, Teknologi, dan Pendidikan Tinggi (Ristekdikti) atau pembuat kebijakan untuk merekomendasikan flipped classroom sebagai model pembelajaran kontemporer pada institusi pendidikan tinggi Indonesia.