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MODIFIKASI PATI GARUT (MARANTHA ARUNDINACEA) DENGAN PERLAKUAN SIKLUS PEMANASAN SUHU TINGGI-PENDINGINAN ( AUTOCLAVING-COOLING CYCLING) UNTUK MENGHASILKAN PATI RESISTEN TIPE III [ARROWROOT (MARANTHA ARUNDINACEA) STARCH MODIFICATION THROUGH AUTOCLAVING-COO ., Sugiyono; Pratiwi, Ratih; Faridah, Didah Nur
Jurnal Teknologi dan Industri Pangan Vol. 20 No. 1 (2009): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The objectives of this research were to modify arrowroot starch to produce resistant starch type III by autoclaving-cooling cycling and to characterize the modified arrowroot starches according to their chemical properties. The research consisted of three steps : arrowroot extraction, starch modification, and chemical characterization. The extraction of arrowroot produced 10.78% starch. Starch modification by autoclaving-cooling cycle(s) was run in 6 different treatments with the aim of observing optimal yield of resistant starch. Resistant starch (RS) was produced through 1, 3, 5 cycle(s) of autoclaving-cooling treatment with different gelatinization periods (15 and 30 minutes autoclaving) for each cycle. Properties analyzed were starch digestibility, fat content, protein content, resistant starch content, and dietary fiber content. Native starch and commercial RS type III (Novelose 330) were also analyzed as a comparison.   Based on the starch digestibility, modified starch from 5 cycles, 15 minutes gelatinization period had the lowest digestibility while modified starch from 3 cycles, 15 minutes gelatinization period had no significant difference with commercial RS type III. Those two modified starches, native starch, and Novelose 330 were chosen for further chemical analysis. The fat content and protein content of the four samples were below 1% (db). Low fat and protein content were required to optimize the RS yield. The modification treatment  increased the dietary fiber content. The RS content of native starch, Novelose 330, modified starch 3 cycles, 15 minutes gelatinization period, and modified starch 5 cycles, 15 minutes gelatinization period were 2.12% (db), 20.80% (db), 10.91% (db), and 12.15% (db), respectively. It means that repeating autoclaving-cooling cycling could increase RS yield up to 6 times.                  
PENGARUH RETROGRADASI DAN PERLAKUAN KELEMBABAN PANAS TERHADAP KADAR PATI RESISTEN TIPE III DALUGA -, Agustina; Faridah, Didah Nur; Jenie, Betty S. L.
Jurnal Teknologi dan Industri Pangan Vol. 27 No. 1 (2016): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (335.953 KB) | DOI: 10.6066/jtip.2016.27.1.78

Abstract

The giant swamp taro starch or daluga corm starch in this study was extracted from corms collected from Siau Island of North Sulawesi Province in Indonesia. Native starch daluga corm has low resistant starch content, so starch modifications method were needed to increase its resistant starch content. The effect of acid hydrolisis, debranching, autoclaving-cooling and heat moisture treatment on the changes of RS3 daluga were studied. The corrm starch was first modified with acid hydrolysis (HA) and debranching with pullulanase (D). The starch was then modified with one cycle autoclaving-cooling (HADAC1x), HADAC1x with heat moisture treatment (HADAC1x+HMT), three cycle autoclaving-cooling (HADAC3x), HADAC3x with heat moisture treatment (HADAC3x+HMT) which increased its RS3 content to 42.37, 40.47, 31.12, and 31.18% (db), respectively. The starch modification method can affect other chemical properties changes such as starch content, amylose content, and reducing sugar content.
SELEKSI BAKTERI ASAM LAKTAT PENGHASIL AMILASE DAN PULULANASE DAN APLIKASINYA PADA FERMENTASI TALAS Setiarto, R. Haryo Bimo; Jenie, Betty Sri Laksmi; Faridah, Didah Nur; Saskiawan, Iwan; -, Sulistiani
Jurnal Teknologi dan Industri Pangan Vol. 26 No. 1 (2015): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (375.854 KB) | DOI: 10.6066/jtip.2015.26.1.80

Abstract

The objective of this study were to select amylase and pululanase producing lactic acid bacteria (LAB) for taro fermentation and to find out the length of fermentation time that will produce short chain polysaccharide. Fourty one LAB isolates were selected based on the amylase and pululanase activity (U/mL). Three isolates of LAB i.e. Lactobacillus plantarum D-240, SU-LS67 and SU-LS59 demonstrated the highest enzyme activities among other strains. The amylase activity for those three isolates was 2.57, 2.70, and 2.50 U/mL, respectively and the pullulanase activity was 2.72, 2.88 and 2.91 U/mL, respectively. Genotypic identification was conducted for strains SU-LS59 and SU-LS67. Strains identification by sequencing the gene encoding 16S rDNA and phylogenetic analysis using Neighbor Joining method showed that both isolates were identical to Leuconostoc mesenteroides NBRC 100496T (AB681194 ) with a bootstrap value of 100%. Either single or mixed culture of L. plantarum D-240 and L. mesenteroides SU-LS 67 were then used as starter in taro fermentation and DP values of the taro starch were examined at various fermentation times (0, 6, 12, 18, 24 h). The results showed that applying 2% mixed culture (108 CFU/mL) of L. plantarum D-240 and L. mesenteroides SU-LS 67) at the ratio of 1:1 as starter in taro fermentation was found more effective than the single cultures due to its ability to hydrolize and generate starch with DP value around 27 after 18 h fermentation. Starch with DP values between 19-29 was considered suitable for the formation of resistant starch (RS) during autoclaving-cooling cycles. This finding might be advantageous as preliminary treatment for the production of RS-rich taro flour through autoclaving-cooling process.
IN VIVO EVALUATION OF PREBIOTIC AND SYNBIOTIC PROPERTIES OF PROCESSED SWEET POTATO PRODUCTS Nuraida, Lilis; ., Hana; Dwiari, Sri Rini; Faridah, Didah Nur
Jurnal Teknologi dan Industri Pangan Vol. 19 No. 2 (2008): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The aims of this research were to investigate the prebiotic and synbiotic properties of sweet potato products (combined with L. casei subsp Rhamnosus for probiotic) in increasing the number of Lactic Acid Bacteria (LAB) and suppressing the number of E. coli and the occurrence of Salmonella in vivo. Some previous study showed that sweet potato is a potent source of prebiotic. The sweet potato products evaluated were sweet potato flakes (SPF) and sweet potato ice cream mix. The in vivo assay used male rat strain Sprague-Dawley. Total microbes, LAB, E. coli and Salmonella in fecal were analyzed before, during and after feeding period. Feeding with SPF as prebiotic, L. casei subsp Rhamnosus as probiotic, and combination of both as synbiotic for ten days were able to increase the number of LAB (0.4-1.1 log CFU/g) and suppress the number of E. coli in rat feces (1.5-1.7 log CFU/g). All of the treatment did not affect the occurrence of Salmonella in rat feces. The treatment of sweet potato ice cream mix as prebiotics and the combination of sweet potato ice cream mix and L. casei subsp. Rhamnosus as synbiotic for ten days did not effect the number of LAB and E. coli in rat feces.Key words: sweet potato, prebiotic, oligosaccharide, probiotic, synbiotic
KARAKTERISASI FISIKOKIMIA NANOKALSIUM HASIL EKSTRAKSI TULANG IKAN NILA MENGGUNAKAN LARUTAN BASA DAN ASAM [PHYSICOCHEMICAL CHARACTERIZATION OF NANO CALCIUM FROM TILAPIA BONE EXTRACTED BY ALKALINE AND ACID SOLUTION] Lekahena, Vanessa; Faridah, Didah Nur; Syarief, Rizal; Peranginangin, R.
Jurnal Teknologi dan Industri Pangan Vol. 25 No. 1 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (759.499 KB) | DOI: 10.6066/jtip.2014.25.1.57

Abstract

KARAKTERISASI FISIKOKIMIA NANOKALSIUM HASIL EKSTRAKSI TULANG IKAN NILA MENGGUNAKAN LARUTAN BASA DAN ASAM[Physicochemical Characterization of Nano Calcium from Tilapia Bone Extracted by Alkaline and Acid Solution]Vanessa Lekahena1)*, Didah Nur Faridah2), Rizal Syarief2) dan R. Peranginangin3) 1) Fakultas Pertanian, Universitas Muhammadiyah Maluku Utara, Ternate2) Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Bogor3) Balai Besar Penelitian dan Pengembangan Pengolahan Produk dan Bioteknologi, Kementerian Kelautan dan Perikanan, Jakarta Diterima 26 Maret 2013 / Disetujui 29 April 2014 ABSTRACT The utilization of tilapia (Oreochromis niloticus) bones as a source of natural calcium was done by alkaline (NaOH) and acid (HCl) extraction. The extraction process aims to soften the bones are milled using disc mill into nano sized calcium powder or nanocalcium. The objective of this research was to study the physicochemical properties of nanocalcium obtained from alkaline and acid extraction of tilapia bones. The results of physicochemical analysis properties of nanocalcium sample showed that nanocalcium extracted by alkaline solution had better properties than that of the untreated sample and nanocalcium extracted by acid solution. The results was indicated by the higher yield, brighter colors, smaller particle size, and calcium content (20.67%) and phosphorus (10.09%) with a ratio of Ca/P = 2.0. The Fourier Transform Infrared (FTIR) spectra profile indicated the presence of  phosphate group (PO43-) at the bands of 469, 563, 603, 961 and 1035 cm-1, and the presence of  apatite carbonate group (CO32-) was  indicated by intense bands at 873, 1416, 1456 and 1563 cm-1. The morphology of the sampel surface of the  alkaline extracted sample was  smoother, denser, and the grain size formed were relatively larger. The formed crystalline phases were HAp (hydroxyapatite), carbonate apatite type A [Ca10(PO4)6CO3] and B [Ca10(PO4)3(CO3)3(OH)2] with crystalline degree of 78.4%.
METABOLISME PREBIOTIK OLEH KANDIDAT PROBIOTIK ISOLAT ASI SEBAGAI DASAR PENGEMBANGAN PRODUK SINBIOTIK [PREBIOTICS METABOLISM BY PROBIOTICS CANDIDATES ISOLATED FROM BREAST MILK AS A BASIS FOR DEVELOPMENT OF SINBIOTICS PRODUCT] Nuraida, Lilis; Mardiana, Nur Rita; Faridah, Didah Nur; ., Hana
Jurnal Teknologi dan Industri Pangan Vol. 22 No. 2 (2011): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Five Lactobacillus strains and two Pediococcus strains isolated from breast milk were evaluated for their ability to metabolite oligosaccharide known as prebiotics, i. e. inulin, FOS, GOS, FOS:GOS (1:9), and inulin:GOS (1:9). The result shows that all Lactobacillus and Pediococcus strains were able to grow in medium with prebiotics as carbon sources. The best growth was observed when GOS or FOS were used as carbon source, while inulin was the worst. The best growth in all oligosaccharides was observed in Lactobacillus R23H, followed by L. rhamnosus B16, L. rhamnosus R23, and L. rhamnosus R14. Further study on R23H (heterofermenter) and R23 (homofermenter) shows that there was no difference in metabolizing oligosaccharides between heterofermenter and homofermenter. GOS was the best prebiotic used by the both lactic acid bacteria as shown by rapid decrease of total sugar. The reducing sugar in medium containing GOS was higher than other oligosaccharides. The decrease of total sugar in medium containing inulin was the slowest, indicating that inulin was the most difficult prebiotic to be metabolized by the lactic acid bacteria. When inulin mixed with GOS in the ratio 1:9, the rate of decreasing of total sugar in the medium was similar to the medium with GOS as single carbon source, however it cannot be distinguished if the role of inulin took place after GOS disappeared. The rate of sugar metabolism was in accordance with the growth of lactic acid bacteria isolates in medium containing prebiotics. L. rhamnosus R23 was used in sinbiotic fermented milk product combine with FOS, inulin, and inulin:GOS (1:9). After fermentation, remaining sugar in skim milk with additional prebiotics was higher than skim milk without additional prebiotics. Remaining sugar on fermented milk product includes the amount of prebiotic that will be used as carbon source in colon. Combination of potential probiotic L. rhamnosus R23 and mixture of inulin:GOS (1:9) could be used to make sinbiotic fermented milk product. 
PROPERTIES OF SUWEG (AMORPHOPHALLUS CAMPANULATUS B1) AND ITS GLICEMIC INDEX Faridah, Didah Nur
Jurnal Teknologi dan Industri Pangan Vol. 16 No. 3 (2005): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Suweg (Amorphophallus campanulatus B1) is an indigenous tuber potentially used as an alternative food source. The objective of the study was to identify the functional properties, i.e. dietary fiber and Glicemic Index (IG) of Suweg by in vivo method using human. The experiment was conducted in three steps i.e suweg flour production, analysis of suweg flour functional properties, and determination of glycemic index (IG) using human. Suweg tuber had high content of dietary fiber (13,71%), and protein (7,20%) but had low fat content (0,28%). Suweg tuber had low IG (42) with low starch in vitro digestibility (61,75%), hence its was categorized as food product with low IG (
CHANGES OF THYMOQUINONE, THYMOL, AND MALONDIALDEHYDE CONTENT OF BLACK CUMIN (NIGELLA SATIVA L.) IN RESPONSE TO INDONESIA TROPICAL ALTITUDE VARIATION Herlina, .; Aziz, Sandra Arifin; Kurniawati, Ani; Faridah, Didah Nur
HAYATI Journal of Biosciences Vol. 24 No. 3 (2017): July 2017
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (454.061 KB) | DOI: 10.4308/hjb.24.3.156

Abstract

Black cumin cultivated in many subtropical regions in the world, including Asia, Middle East, and North Africa. The most active constituent of black cumin is thymoquinone representing 18.4%?24% of the volatile oil and thymol. Data about thymoquinone and thymol came from the country of origin, but no data from tropical region. This study aimed to analyze the production of chlorophyll, thymoquinone, thymol, and malondialdehyde from black cumin cultivated at three altitudes of Indonesian tropical region. The result showed that Kuwait accession cultivated at middle altitude contains the highest levels of thymoquinone (2940.43 mg/kg), and the highest levels of thymol were found in India accession cultivated at high altitude (141.46 mg/kg). Data showed that the level of malondialdehyde at low (220 meter above sea level [masl]) and middle (560 masl) altitudes is higher than high (1.280 masl) altitude.
PENDUGAAN UMUR SIMPAN DENGAN METODE ACCELERATED SHELF-LIFE TESTING PADA PRODUK BANDREK INSTAN DAN SIRUP BUAH PALA (MYRISTICA FRAGRANS) Faridah, Didah Nur; Yasni, Sedarnawati; Suswantinah, Antin; Aryani, Ghesi Wuri
Jurnal Ilmu Pertanian Indonesia Vol. 18 No. 3 (2013): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Traditional beverages can be made from the spices and can be processed further as instant powder drinks such as bandrek drink. The expiration date statement on the food packaging is a mandatory according to the Food Act No.7/1996 and the Government Regulation No. 69/1999 about Food Labelling and Advertising. The aim of this research was to estimate or to predict the shelf-life of instant bandrek drink produced at Sinarsari village and nutmeg syrup at Dramaga village, Dramaga district, Bogor regency. Shelf-life estimation of the two products was done by accelerated shelf-life testing method. Instant bandrek was predicted to have shelf-life for 341 days (11 months) when stored at 80% RH, 30 °C, while the nutmeg syrup would be expired in 34-47 days when stored at 30 °C.
KARAKTERISTIK KIMIA KONJUGAT ISOLAT PROTEIN KEDELAI-LAKTOSA YANG BERPOTENSI DALAM PENURUNAN ALERGENISITAS Asyhari, Muhammad Hasriandy; Palupi, Nurheni Sri; Faridah, Didah Nur
Jurnal Teknologi dan Industri Pangan Vol. 29 No. 1 (2018): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (429.012 KB) | DOI: 10.6066/jtip.2018.29.1.39

Abstract

Soy protein isolate (SPI) is a product from soybeans with great functionality thus is widely used as an ingredient in any formulation by food and beverage industries. Glycation is commonly done to improve protein functionality and reduce its allergenicity. This research aims to analyze the influence of incubation time of SPI and lactose on the degree of glycation of SPI-lactose conjugates, free amino acid and lactose bases, and to identify the molecular weight and intensity of the protein band of the SPI-lactose conjugate. SPIs were obtained by pH adjustment. The glycation of SPI and lactose was undertaken at pH 9.5, 95°C for 0, 30, 60 and 90 minutes. The determination of the glycation degree of the SPI-lactose conjugate was carried out using several methods, i.e. TBA-based method, HPLC and Bradford analysis. The profiles of the protein molecular weight was analyzed using SDS-PAGE. The results revealed that longer incubation time induced a greater degree of glycation. SDS-PAGE showed that the soybean sample had 13 protein bands with molecular weights ranging from 11.8 to 170.2 kDa. Glycation of SPI with lactose at 95°C could remove or lower the intensity of protein bands with molecular weight of 66.6; 56.1 and 30.9 kDa, which were presumed to be the major allergens in soybean.