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AKT–THE MAMMALIAN TARGET OF RAPAMYCIN (MTOR) PATHWAY INHIBITION INCREASES CERVICAL CANCER CELL CHEMOSENSITIVITY TO ACTIVE FORM OF IRINOTECAN (SN-38) Septiani, Leri; Hidayat, Yudi Mulyana; Effendi, Yusuf Sulaeman; Djuwantono, Tono; Luftimas, Dimas Erlangga; Faried, Ahmad
International Journal of Integrated Health Sciences Vol 1, No 1 (2013)
Publisher : International Journal of Integrated Health Sciences

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Objective: To investigate the molecular pathway of the cytotoxic effect of SN-38 in human cervical cancer cell lines.Methods: Two human cervical cancer cell lines were treated with various concentrations of irinotecan for 24?72 hours and the sensitivity was analysed using the MTT assay. Apoptosis was further observed through microscopic examinations. The protein expression was determined using Western blot analysis.  Results: CaSki cells demonstrated the highest sensitivity to SN-38, whereas HeLa cells showed the lowest. In cervical cancer cells, SN-38 induced apoptosis through an intrinsic- and extrinsic-pathways. In addition, we showed that SN-38 downregulated the phosphorylation of Akt-mTOR pathways in CaSki cells, but not in HeLa cells. Interestingly, in HeLa cells, which were more suggestive of a resistant phenotype, pre-treatment with LY294002 and rapamycin inhibited activation of Akt-mTOR signaling and significantly enhanced the sensitivity of HeLa cells to SN-38. Conclusions: Irinotecan exerts its anti-neoplastic effects on cervical cancer cells by inducing apoptosis through caspase-cascade. Inhibition of Akt-mTOR, LY294002 and rapamycin, which is targeted to Akt-mTOR pathways, may sensitize irinotecan-resistant cervical cancer cells.Keywords: Akt-mTOR pathways anti-neoplastic drugs, cervix cancer cells, LY294002, rapamycin DOI: 10.15850/ijihs.v1n1.103
INCIDENCE OF CAVUM SEPTUM PELLUCIDUM AND CAVUM VERGAE IN DR. HASAN SADIKIN GENERAL HOSPITAL, BANDUNG, INDONESIA Faried, Ahmad; Soeprajogo, Adolf Setiabudi; Melia, Regina; Arifin, Muhammad Zafrullah
International Journal of Integrated Health Sciences Vol 5, No 1 (2017)
Publisher : International Journal of Integrated Health Sciences

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Objective: To describe the cavum septum pellucidum (CSP) and cavum vergae (CV) findings. Methods: Thirteen samples were recruited in the study. The study was conducted at the Department of Neurosurgery, Dr. Hasan Sadikin Hospital, Bandung, Indonesia in the period of 2015?2016. Thirteen samples were recruited for the study. Cavum septum pellucidum and CV spaces are relatively rare. During neurology and neurosurgery examination of patients with head computed tomography (CT) scan and magnetic resonance imaging (MRI), CSP and CV were discovered accidentally. The incidence of these unique spaces reported from many countries, but have never been reported from Indonesia.Results: This is the first-time reported incidental finding of CSP and CV from Indonesia, 13 cases in one-year period. Since the presence of CSP and CV is usually asymptomatic, clinician seem don?t really care to put it in their report.Conclusions: The presence of CSP and CV as a common incidental finding can be more revealed in our country in years to come and hopefully can be more studied in term of anatomical landmark on neurosurgical field.Keywords: Cavum septum pellucidum, cavum vergae, incidental finding DOI: 10.15850/ijihs.v5n1.964
MODIFIKASI METODE ISOLASI SEL ENDOTEL PEMBULUH DARAH OTAK (EPDO) TIKUS: TEKNIK DASAR KULTUR SEL PRIMER DI BIDANG NEUROSAINS Faried, Ahmad; Zafrullah Arifin, Muhammad; Sutiono, Agung Budi; Halim, Danny; Djuwantono, Tono; Achmad, Tri Hanggono
Majalah Kedokteran Bandung Vol 42, No 4
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Metode konvensional isolasi sel endotel pembuluh darah otak (EPDO) masih tergolong sulit, sehingga upaya mendapatkan populasi murni sel ini adalah tantangan. Pada penelitian ini dilakukan isolasi endotel dari tikus Wistar dan mencit C57/Bl6, berdasarkan protokol the care and use of laboratory animals, Universitas Gunma, Jepang. Modifikasi metode isolasi adalah menggunakan gradasi bovine serum albumin (BSA), bukan Dextran-70 yang umumnya dipakai, untuk memisahkan sel EPDO yang bersatu menjadi sel EPDO tunggal. Penelitian ini dilakukan di laboratorium sel kultur, Universitas Padjadjaran bekerjasama dengan Universitas Gunma, Jepang, Januari 2008?Juni 2009. Uji hasil isolasi dan karakteristik sel EPDO dilakukan dengan teknik imunofloresen. Ekspresi tight junction ZO-1, menunjukkan sel EPDO membentuk selapis sel utuh, rapat, tidak bertumpuk dan kompak, sesuai dengan karakteristik dinding EPDO. Fenotip sel EPDO dikonfirmasi dengan acethylated LDL, faktor von Willebrand dan CD31. Penghancuran kapiler dengan collagenase/dispase masih menghasilkan populasi sel yang terkontaminasi perisit. Kontaminasi dimurnikan dengan menggunakan puromycin, tingkat pemurnian sel EPDO mencapai 98,3%. Simpulan, teknik modifikasi berhasil mengisolasi sel EPDO tikus dan mencit, tanpa melakukan intervensi genetik. Puromycin dapat digunakan untuk memurnikan sel EPDO. [MKB. 2010;42(4):161?8].Kata kunci: Metode modifikasi isolasi sel EPDO, pembuluh sawar otak, teknik pemurnian Isolation Modified-Method of Mouse-Brain Microvessel Endothelial Cells: Primary Cell Culture Technique in NeuroscienceIsolation method to obtain pure BMVECs is hard to be done consistently and remains a challenge. In this study, we isolated BMVECs from Wistar rat and C57/Bl6 mouse from Japan SLC. All procedures performed according to guidelines for the care and use of laboratory animals of Gunma University, Japan. The modification of isolation method was using bovine serum albumin (BSA) gradation, not Dextran-70 in which generally used, to separate clusters of BMVECs into single cell. This study was done at Universitas Padjadjaran, in colaboration with Gunma University, Japan, January 2008?June 2009. Further,characteristic and purification results were proven by imunofluorescene staining. The results showed that staining of tight junction, ZO-1, formed a monolayer, tightly packed, non-overlapping and contact-inhibited BMVECs, as expected for a vessel wall endothelial. ECs phenotype confirmed by acethylated LDL, von Willebrand and CD31. The digestion of capillaries generated contaminating pericytes. Contamination was purified using puromycin and the results considered satisfactory (98.3%). In conclusion, our modification procedure allows the isolation of primary rat and mouse BMVECs, which form an endothelial-like monolayer in few days. Puromycin can be used for purification of primary rat and mouse BMVECs. [MKB. 2010;42(4):161?8].Key words: Blood brain barrier, isolation modified-method of mouse-BMVECs, purification methods DOI: http://dx.doi.org/10.15395/mkb.v42n4.30
COMPARISON OF HISTOLOGICAL CHARACTERISTICS OF DRIED AND FRESH AMNION MEMBRANES AND DURA MATER IN NON-HUMAN PRIMATE (MACACA FASCICULARIS) Indah, Astrina Rosaria; Bolly, Hendrikus Masang B.; Faried, Ahmad; Arifin, Muhammad Zafrullah; Achadiyani, Achadiyani; Wirakusumah, Firman Fuad
Majalah Kedokteran Bandung Vol 51, No 1 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v51n1.1651

Abstract

This study aimed to characterize the histological properties of dry-lyophilized amniotic membrane, fresh amniotic membrane (AM), and duramater membrane in search for a biologically-derived material suitable for meninges surface reconstruction. This descriptive study was conducted at the Unit-3 Laboratory of Animal Test of PT. Bio Farma (Persero), Bandung and Cell Biology Laboratory of the Faculty of Medicine, Universitas Padjadjaran Bandung. This study was conducted from 2018-2019. Fresh Macacafascicularis placenta from healthy donors,classified as specific pathogen-free for TB, SIV, SV40, Polio type 1,2,3, Foamy virus and Herpes B virus, were obtained from selected caesarean sections.The harvested dried and fresh AM and duramater membrane were stained with hematoxylin-eosin and prepared for characterization. Histological examination of dry-lyophilized and fresh AM showed similar results. Histologically, AM is represented by a single layer of metabolically-active cuboidal to columnar epithelium with microvilli firmly attached to a basement membrane and an avascular and relatively sparsely populated stroma. Meninges layers consists of 3 layers: duramater, arachnoid membrane, and piamater. Most of these cells have the same characteristics as fibroblasts, including long organelles and nuclei with various levels of fibril formation. The histological study of amniotic membrane and duramater membrane shows comparable results. The AM is a biologically-derived material suitable for meninges surface reconstruction since its histological structure is somewhat similar to that of the duramater. Its structure is ideal for replacing duramater since it has several characteristics, such as having hygroscopic properties, good biocompatibility, relatively easy to apply, and inexpensive. Key words: Dried and fresh amnion membranes, duramater membranes, non-human primate Karakteristik Histologis Membran Amnion Jenis Kering dan Segar dengan Membran Duramater pada Primata Non-Human Macaca fascicularisPenelitian ini bertujuan mengetahui karakteristik histologis membran amnion kering yang diliofilisasi, membran amnion segar, dan duramater, dalam rangka mencari bahan biologis yang cocok untuk rekonstruksi permukaan meninges. Penelitian deskriptif dilakukan di Laboratorium Hewan Uji PT. Bio Farma dan Laboratorium Biologi Sel FK Universitas Padjadjaran periode 2018-2019. Plasenta Primata non-human Macaca fascicularis segar dari donor sehat, yang bebas dari pathogen spesifik TB, SIV, SV40, Polio tipe 1, 2, 3, virus Foamy dan virus Herpes B, diperoleh dari seksio sesarea. Kemudian, dilakukan pewarnaan dengan hematoxylin-eosin untuk membran amnion kering dan segar, serta membran duramater untuk mengetahui karakterisasi histologisnya. Pemeriksaan histologis membran amnion kering-yang aktif bermetabolisme hingga kolumnar dengan mikrovili; melekat kuat pada membran basal dan stroma yang avaskular dan relatif jarang. Lapisan Meninges terdiri dari 3 lapisan: duramater, arachnoid dan piamater. Sebagian besar sel-sel ini memiliki karakteristik yang sama dengan fibroblas. Studi histologis membran amnion dan membran duramater memiliki struktur yang relatif serupa. Membran amnion adalah material yang secara biologis cocok untuk rekonstruksi permukaan meningen, karena struktur histologinya agak mirip dengan duramater. Oleh karena itu secara struktur, membran amnion ideal untuk menggantikan duramater karena memiliki beberapa karakteristik seperti sifat higroskopis, biokompatibilitas baik, mudah diterapkan, dan murah.Kata kunci: Membran amnion segar dan kering, membran duramater, primate non-human
A NEW BEGINNING FOR MAJALAH KEDOKTERAN BANDUNG (MKB) IN 2019 Faried, Ahmad; Ghrahani, Reni
Majalah Kedokteran Bandung Vol 50, No 4 (2018)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v50n4.1589

Abstract

[no abstract available]
AMYOTROPHIC LATERAL SCLEROSIS: FIRST CASE REPORT IN DEPARTMENT OF NEUROSURGERY, FACULTY OF MEDICINE, UNIVERSITAS PADJADJARAN, BANDUNG Faried, Ahmad; Saputra, Priandana Adya Eka; Dhuha, Alief; Arifin, Muhammad Zafrullah
International Journal of Integrated Health Sciences Vol 7, No 1 (2019)
Publisher : International Journal of Integrated Health Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/ijihs.v7n1.1237

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Objective: Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease that is incurable and results in paralysis of the muscles. Electromyography (EMG) is used to diagnosis amyotrophic lateral sclerosis. Although recently there is no cure for ALS, knowledge has increased significantly in the past several years and is helping those who are newly diagnosed.Methods: This study reported for the first time in Department of Neurosurgery, Faculty of Medicine, Universitas Padjadjaran-Dr. Hasan Sadikin General Hospital, Bandung a 58-year old man who was presented in the institution with a history of weakness of both lower extremities for four months preceded by weakness of both upper extremities since the previous month. There was no history of any medical illness or any chronic medication. The patient then underwent EMG studies, followed by muscle biopsy.Result: Electromyography and histopathological results confirmed a diagnosis of ALS.Conclusion: This case was so exceptional, since ALS occurrence in Neurosurgery Centre is extremely rare, and the diagnosis can only be established through EMG and histopathological of muscle biopsy studies.Keywords: The fist reported of ALS in Neurosurgery Bandung, electromyography studies 
STRATEGY FOR THE USE OF ERYTHROPOETIN ALPHA TO MAINTAIN HEMOGLOBIN LEVEL IN BREAST CANCER PATIENT TREATED WITH ANTHRACYCLINE-BASE OF ADJUVANT CHEMOTHERAPY Achmad, Dimyati; Hariady, Yusuf Hariady; Isakh, Benny; Abdurrahman, Maman; Faried, Ahmad
International Journal of Integrated Health Sciences Vol 1, No 1 (2013)
Publisher : International Journal of Integrated Health Sciences

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Objective: To evaluate the value of erythropoietin alpha (epoetin) administration, as an alternative treatment of anemia in the operable breast cancer patients.Methods: This is a multicenter phase III randomized clinical trial to evaluate the value of epoetin administration among anemic breast cancer patients who are undergoing anthracyclin-based adjuvant chemotherapy. Sixty four patients were incuded in this trial with initial hemoglobin (Hb) level of 10?12 g/dL. The patients were randomly distributed into two groups: one group received aministration of 40,000 IU epoetin/week for six times a week after operation and the other did not.  In the third week after the operation, both groups were started on a 6 cycles of adjuvant chemotherapy with three weeks intervals. Hb levels were evaluated during every chemotherapy cycle. Results: The Hb levels in the epoetin group were always above 10 g/dL up until the end of the sixth chemotherapy cycle or until the twenty first week post operation without blood transfusion. Conclusions: The administration of epoetin 3 weeks prior and 3 weeks after the first cycles of chemotherapy, maintains a sufficient/normal Hb level in breast cancer patients receiving anthracycline-based chemotherapy.Keywords: Anthracyclin-based chemotherapy, breast cancer, erythropoietin alpha DOI: 10.15850/ijihs.v1n1.102
SOYBEAN IS A GOOD SOURCE OF PROTEIN. IT HAS TWO MAJOR FRACTIONS, B-CONGLYCININ (7S) AND GLYCININ (11S). B-CONGLYCININ’S FUNCTION WAS KNOWN TO SUPPRESS FOOD INTAKE, AND THIS EFFECT MAY BE DUE TO STIMULATING ENDOGENOUS CHOLECYSTOKININ (CCK) RELEASE. THE AIMS OF THIS STUDY WERE TO DETERMINE THE HIGHEST CONTENT OF TOTAL B-CONGLYCININ AND B-CONGLYCININ SUB UNIT-B LEVEL OBTAINED FROM TWO VARIETIES OF SOYBEAN I.E. WILIS AND DETAM 1 VARIETIES USING DIFFERENT PREPARATION AND EXTRACTION METHODS. THESE T HIDAYAT, MEILINAH; SUJATNO, MUCHTAN; SUTADIPURA, NUGRAHA; SETIAWAN, .; FARIED, AHMAD
HAYATI Journal of Biosciences Vol. 18 No. 1 (2011): March 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.1.37

Abstract

Soybean is a good source of protein. It has two major fractions, b-conglycinin (7S) and glycinin (11S). b-conglycinin?s function was known to suppress food intake, and this effect may be due to stimulating endogenous cholecystokinin (CCK) release. The aims of this study were to determine the highest content of total b-conglycinin and b-conglycinin sub unit-b level obtained from two varieties of soybean i.e. Wilis and Detam 1 varieties using different preparation and extraction methods. These two soybean varieties were prepared into tempeh. Then the seed and tempeh were extracted using Deak and Panthee methods. There were six extracts analysed using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE) and coomassie brilliant blue (CBB) staining. The result was shown that Detam variety and raw seed contained the highest total b-conglycinin level. And Panthee method was the best method for extraction of total b-conglycinin, while Deak method was the best method for extraction of b-conglycinin subunit-b.
CHARACTERISTICS AND COMPLICATIONS OF TUBERCULOUS MENINGITIS PATIENTS WITH HYDROCHEPALUS UNDERGONE FLUID DIVERSION IN DR. HASAN SADIKIN GENERAL HOSPITAL, BANDUNG Novalia, Adriani Lawrencia; Berbudi, Afiat; Faried, Ahmad; Nataprawira, Heda Melinda
International Journal of Integrated Health Sciences Vol 6, No 1 (2018)
Publisher : International Journal of Integrated Health Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/ijihs.v6n1.1048

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Objective: To describe the clinical characteristics and complications observed in tuberculous meningitis (TBM) patients with hydrocephalus who had undergone fluid diversion management.Methods: This was a cross-sectional descriptive observational study involving 28 TBM patients with hydrocephalus aged 0?5 years who had undergone ventriculoperitoneal shunt or extraventricular drainage in the period of July 2011 to July 2016 in Dr. Hasan Sadikin General Hospital, Bandung. Age, gender, head circumferences, nutritional status, and classical characteristics such as sunset eye, frontal bossing, cracked pot sign, venectation as well as complications such as infection, phlebitis, and exposed shunt documented in the hospital medical records were analyzed.Result: The study discovered that infant group was predominant (21/28). Some of the patients had macrocephalus (7/28) and 18 had good nutrition status (18/28). There were 4 patients with frontal bossing characteristics (4/28) and almost none was found with others classic hydrocephalus characteristics. This study found no complication such as infection, phlebitis, and exposed shunt in TBM patients with hydrocephalus who had undergone fluid diversion therapy in Dr. Hasan Sadikin General Hospital, Bandung.Conclusion: One of the most dominan characteristics of TBM patients with hydrocephalus is frontal bossing. Tuberculous meningitis patients with hydrocephalus in our center did not show any fluid diversion-related complications such as infection, phlebitis, or exposed shunt.Keywords: Tuberculous meningitis, hydrocephalus, fluid diversion DOI: 10.15850/ijihs.v6n1.1048
ANTERIOR TRANSPETROSAL UNTUK LESI PADA FOSA KRANIALIS MEDIA DAN POSTERIOR: REVIEW LITERATUR DAN DISEKSI KADAVER Arifin, Muhammad Zafrullah; Sutiono, Agung Budi; Faried, Ahmad; Kawase, Takeshi; Wirjomartani, Beny Atmadja; Wiriadisastra, Kahdar
Majalah Kedokteran Bandung Vol 42, No 2
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Reseksi selektif pada bagian anterior piramid os petrosus (segitiga Kawase), dengan terlebih dahulu melakukan kraniotomi pada dinding lateral fosa kranialis media, dapat memberikan lapang pandang bedah yang cukup luas dari parasellar, clivus dan cerebellopontine angle (CPA), tanpa mengorbankan struktur organ pendengaran dalam (internal acoustics organ). Keuntungan utama teknik anterior transpetrosal ini adalah dapat langsung mengakses tumor yang melekat pada klivus melalui rongga kecil yang dibuat pada apeks os petrosus. Pengangkatan tumor dengan perdarahan yang minimal dapat dicapai dengan melakukan koagulasi pada arteri tentorium dan akses langsung ke arah anterior kanalis auditorius internus, juga dapat mengurangi cedera pada nervus fasialis dan vestibulokoklearis. Keuntungan lainnya, yaitu, rendahnya risiko komplikasi perdarahan vena, karena teknik ini tidak mengekspos sinus sigmoid ataupun vena Labbe. [MKB. 2010;42(2):86-91].Kata kunci: Anterior transpetrosal, fosa media dan posterior, diseksi kadaverAnterior Transpetrosal for Lession in Middle and Posterior Fossa: Literature Review and Cadaver DissectionResection of the anterior part of pyramid through the middle fossa craniotomy will give us a surgical field of the parasellar, clivus and cerebellopontine angle without sacrificing the auditory structure. The advantage of the anterior transpetrosal approach is the direct access to tumors that attached to the clivus via a keyhole created on the petrosus apex. Bloodless tumor removal can be achieved by detachment of the tentorial artery and direct access of the anterior internal auditory canal also can minimize the injury of the cranial nerve facialis and vestibulocochlearis. Another advantage is the low risk of venous damages since this approach is not exposing the sigmoid sinus and the vein of Labbe. [MKB. 2010;42(2):86-91].Key words: Anterior transpetrosal, middle and posterior fossa, cadaver dissection DOI: http://dx.doi.org/10.15395/mkb.v42n2.219
Co-Authors . SETIAWAN Achadiyani Achadiyani, Achadiyani Afifah, Ervi Agung Budi Sutiono Agustina, Hasrayanti Ajeng Diantini, Ajeng Angliana Chouw, Angliana Benny Isakh Beny Atmadja Wirjomartani Berbudi, Afiat Bolly, Hendrikus Bolly, Hendrikus Masang B. Bolly, Hendrikus Masang Ban Budiman, Astrid Tamara Maajid Cynthia R. Sartika Danny Halim Dewi, Dwi A. P. Dhuha, Alief Diana Krisanti Jasaputra Dimas Erlangga Luftimas Dimyati Achmad Endang Sjamsudin Farid Farid Yudoyono Farid Yudoyono Ferry, Bilzardy Firman Fuad Wirakusumah Firman Priguna Tjahjono, Firman Priguna Hadian, Rizkiditia Nugraha Hanna Sari Widya Kusuma, Hanna Sari Widya Harry Murti Heda Melinda Nataprawira Hermin Aminah Usman Hernowo, Bethy Surjawathy Ida Parwati Imam Hidayat Indah, Astrina Rosaria Indra Bachtiar Julius July Kahdar Wiriadisastra Karima, Amira Puti Koyama, Hiroshi Kusdiansah, Muhammad Leri Septiani Lubis, Billy Parulian Maman Abdurrahman MEILINAH HIDAYAT Melia, Regina Mochammad Aris Widodo MUCHTAN SUJATNO Muhammad Z. Arifin Muhammad Zafrullah Arifin Muhammad Zafrullah Arifin Mutakin Mutakin Novalia, Adriani Lawrencia Noverina, Rachmawati NUGRAHA SUTADIPURA Priandana Adya Eka Saputra Putera, Bayu W. Renaldi Prasetia, Renaldi Reni Ghrahani Rihibiha, Dwi Davidson Riswandani, Julia Rizal Rizal Rizky Abdulah, Rizky Rully Hanafi Dahlan Sastrawan, Agus Dwi Sevline Esthetia Ompusunggu Sobana, Mirna Soeprajogo, Adolf Setiabudi Sri Suryanti Suryana, Nida SUTIMAN BAMBANG SUMITRO Takayuki Ohira Takeshi Kawase Tono Djuwantono Tri Hanggono Achmad WAHYU WIDOWATI Wirjomartani, Benny Atmadja Yembise, Trajanus Laurens Yudi Mulyana Hidayat Yusuf Hariady Hariady Yusuf Sulaeman Effendi