Articles

Found 21 Documents
Search

KARAKTER RHIZOBAKTERI PELARUT FOSFAT POTENSIAL DARI RHIZOSFER TUMBUHAN MANGROVE TELUK AWUR KABUPATEN JEPARA SECARA MIKROBIOLOGI Oktaviani, Eka; Lunggani, Arina Tri; Ferniah, Rejeki Siti
Jurnal Ilmu Lingkungan Vol 18, No 1 (2020): April 2020
Publisher : Program Studi Ilmu Lingkungan,Program Pascasarjana, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.13 KB) | DOI: 10.14710/jil.18.1.58-66

Abstract

Ekosistem mangrove Teluk Awur yang terletak di Kabupaten Jepara, Provinsi Jawa Tengah, merupakan  salah satu ekosistem mangrove yang mengalami kerusakan cukup parah karena perluasan lahan budidaya ikan (tambak), sehingga mendorong terjadinya erosi pantai. Peremajaan kembali dan konservasi ekosistem mangrove dapat dilakukan dengan menggunakan bakteri tanah yang mampu mendukung pertumbuhan tanaman atau yang biasa disebut Plant Growth Promoting Rhizobacteria (PGPR). Salah satu mekanisme pendukung pertumbuhan tanaman oleh kelompok PGPR adalah dengan aktivitas pelarutan fosfat karena fosfat dalam tanah berada dalam bentuk yang sulit diserap oleh tanaman. Penelitian ini bertujuan untuk memperoleh isolat Rhizobakteri pelarut fosfat yang unggul dalam melarutkan fosfat secara in-vitro dan mengetahui karakter isolat yang diperoleh. Isolasi dan penapisan rhizobakteri pelarut fosfat dilakukan menggunakan medium Pikovskaya agar. Karakterisasi isolat potensial dilakukan secara mikrobiologi dan atau uji aktivitas biokimia. Hasil penelitian menunjukkan bahwa rhizobakteri pelarut fosfat potensial yang berhasil diisolasi, secara mikrobiologi teridentifikasi ke dalam genus Enterobacter.
ISOLASI KHAMIR FERMENTATIF DARI BATANG TANAMAN TEBU (SACCHARUM OFFICINARUM. L) DAN HASIL IDENTIFIKASINYA BERDASARKAN SEKUENS INTERNAL TRANSCRIBED SPACER Anggraini, Ika Anggraini; Ferniah, Rejeki Siti; Kusdiyantini, Endang
Berkala Bioteknologi Volume 2 Nomor 2 November 2019
Publisher : Berkala Bioteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (336.023 KB)

Abstract

Yeast is a single-celled fungus that acts as epiphytes, endophytes or parasites. Yeast is divided into fermentative yeast and oxidative yeast. Fermentative yeast can produce primary and secondary metabolites. The role of fermentative yeast is widely used in the food industry, health and energy, so necessary to be explore fermentative yeast from sugarcane stems. The purpose of this study was to isolate fermentative yeast from sugarcane stems and identify molecular yeast based on the Internal Transcribed Spacer sequences (ITS). Isolation of epiphytic and endophytic yeast was carried out by spread plate of water soak sugarcane and sugar cane juice. Yeast isolation using 2 media, PDA and YGP with chloramphenicol. Morphological characterization was carried out by observing macroscopic and microscopic characteristics. Biochemical characterization was carried out by carbohydrate fermentation test and 50% glucose media growth test. Selected isolates were molecularly identified using Internal Transcribed Spacer (ITS) sequences. Primers used are ITS 1 and ITS 4. Phylogenetic analysis using Neighbor Joining from MEGA-6 program. The results of isolation obtained 7 yeast isolates characterized morphologically and biochemically. The based result of morphology and biochemical characterization were found 1 selected isolate with name Ed 1B. Selected yeast isolate have characteristics are round colonies, creamy white, shiny surface, raised elevation, wavy edges, ovoid cell shape, cell diameter 4,74µm, budding, glucose fermentation and sucrose fermentation, but not for lactose and grow well of 50% glucose media. The results of the Basic Alignment Search Tools (BLAST) are Ed 1B isolates had 99% homology with Kodamaea ohmeri species. Key Words :  Sugarcane (Saccharum officinarum. L.), Yeast, Molecular Identification
PENGENDALIAN HAYATI PENYAKIT HAWAR DAUN TANAMAN KENTANG DENGAN AGENS HAYATI JAMUR-JAMUR ANTAGONIS ISOLAT LOKAL Purwantisari, Susiana -; Ferniah, Rejeki Siti; Raharjo, Budi -
Bioma : Berkala Ilmiah Biologi Vol. 10, No. 2, Tahun 2008
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1981.199 KB) | DOI: 10.14710/bioma.10.2.51-57

Abstract

Penyakit hawar daun tanaman kentang atau yang oleh petani di Kedu, Wonosobo disebut Lodoh merupakanpenyakit yang paling serius di antara penyakit dan hama yang menyerang tanaman kentang di Indonesia. Penyakitlodoh ini disebabkan oleh serangan jamur patogen ganas Phytophthora infestans yang dapat menurunkan produksikentang hingga 90% dari total produksi kentang dalam waktu yang amat singkat. Sampai saat ini kapang patogenpenyebab penyakit busuk batang dan daun tanaman kentang tersebut masih merupakan masalah krusial dan belumada fungisida yang benar-benar efektif terhadap penyakit tersebut. Penelitian ini bertujuan mengoleksi danmengidentifikasi jamur-jamur tanah isolat lokal yang bersifat antagonis terhadap patogen penyebab penyakit busukdaun dan umbi tanaman kentang. Hasil penelitian menunjukkan bahwa penyebab penyakit busuk daun dan umbitanaman kentang di daerah sentra pembibitan tanaman kentang di Kedu Temanggung Jawa Tengah adalahPhytophthora infestans. Terdapat 17 isolat jamur isolat lokal yang dapat diisolasi dari tanah di sentra pembibitantanaman kentang tersebut. Dari 17 isolat jamur ini dapat dikelompokkan menjadi 4 kelompok isolat yang berbedamorfologi koloninya. Pengamatan secara mikroskopis menunjukkan bahwa dari 4 kelompok jamur tanah tersebutadalah dari marga Trichoderma spp, Aspergillus sp, Penicillium sp dan Phytophthora infestans.
PRODUKSI DAN EKSTRAKSI INHIBITOR ALFA GLUKOSIDASE DARI ISOLAT AKTINOMISET JP-3 Pujiyanto, Sri; Ferniah, Rejeki Siti; S, Sunarno
Bioma : Berkala Ilmiah Biologi Vol. 17, No.2, Tahun 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.608 KB) | DOI: 10.14710/bioma.17.2.123-129

Abstract

Alpha-glucosidase inhibitors are compounds that can prevent the digestion of complex carbohydrates into glucose, so potentially used as a diabetes drug. This study aims to examine the production and extraction of alpha-glucosidase inhibitor compound from Isolate Aktinomiset JP-3 from the sea. The supernatant obtained from the culture of the JP-3 isolate was extracted using various solvents to obtain the active compound. The solvents used were chloroform, methanol, and ethyl acetate. An assay of inhibitor activity of the ?-glucosidase using p-nitrophenyl ?-D-glucopyranoside substrate. The activity of the enzyme is measured based on the absorbance of p-nitrophenol produced from the breaking reaction of the substrate. The results showed that extraction of alpha-glucosidase inhibitor compound with ethyl acetate yielded extract with highest inhibitor activity. Keywords: alpha-glucosidase inhibitors, actinomycetes, diabetes, extraction, fractionation
INTERAKSI KAPANG PATOGEN FUSARIUM OXYSPORUM DENGAN BAKTERI KITINOLITIK RIZOSFER TANAMAN JAHE DAN PISANG Ferniah, Rejeki Siti; Pujiyanto, Sri; Purwantisari, Susiana; Supriyadi, Supriyadi
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (51.542 KB) | DOI: 10.31258/jnat.14.1.56-60

Abstract

Fusarium oxysporum is a pathogenic fungi for many plants. The fungi have chitin cell wall that can be degraded by chitinase fromchitinolytic bacteria. Aim of this research is determine how the interaction between the bacteria and F.oxysporum. Bacteria were isolatedfrom plant rizosfere. Chitinolytic activity were measured based on the clear zone around the colony in chitin medium. Bacteria and fungiinteraction were determined by an antagonistic test. This research showed that there were 9 chitinolytic bacteria. J4 and P3 had highchitinolytic index, that are 3 and 3.33, respectively. The two isolates antagonist to F.oxysporum, which the bacteria prevent growth of thefungi. The J4 and P3 are alternative biofungicide for F.oxysporum.
OPTIMASI ISOLASI DNA CABAI (CAPSICUM ANNUUM L.) BERDASAR PERBEDAAN KUALITAS DAN KUANTITAS DAUN SERTA TEKNIK PENGGERUSAN Ferniah, Rejeki Siti; Pujiyanto, Sri
Bioma : Berkala Ilmiah Biologi Vol. 15, No.1, Tahun 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (311.999 KB) | DOI: 10.14710/bioma.15.1.14-19

Abstract

Complete genome of chili has not been reported.The first step to study the genome is DNA isolation, so it is necessary to optimize the protocol to get an optimum DNA. This research aimed to optimize chili DNA isolation by variate the quantity and quality of chili leaves as row material and variate the grinding technique. DNA isolation was done using commercial kit without liquid nitrogen, and analyzed by agarose gel electrophoresis. The results showed that frozen chili leaf yields more DNA than fresh leaf, 0,1 g of leaf got optimum DNA, and grinding in mortar was better than in microtube.   Key words: DNA,  isolation, Capsicum annuum
Indonesian red chilli (Capsicum annuum L.) capsaicin and its correlation with their responses to pathogenic Fusarium oxysporum Ferniah, Rejeki Siti; Pujiyanto, Sri; Kusumaningrum, Hermin Pancasakti
NICHE Journal of Tropical Biology Vol. 1, No. 2, Year 2018
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (185.917 KB) | DOI: 10.14710/niche.1.2.7-12

Abstract

Red chili is a commercial crop for the food industry in Indonesia. There are some categories of red chili based on their pungency. The hot chili usually has more capsaicin than the sweet chili. Some cultivars may have more resistance to pathogen infection than the others. This research aimed to analyze the disease resistance of red chili cultivars from Indonesia against pathogenic Fusariumoxysporum and the correlation with capsaicin contents. Disease resistance was examined by determination of the Disease Severity Index (DSI) 15 dpi (days post inoculation). The correlation was analyzed by the regression coefficient. The result showed that the most resistance cultivar against F. oxysporum was Branang, while Lembang-1displayed the contrary. There was not a correlation of capsaicin content with the chili resistance to F. oxysporum.
PRODUKSI INULINASE PICHIA ALNI DUCC-W4 PADA TEPUNG UMBI DAHLIA (DAHLIA VARIABILIS WILLD) DENGAN VARIASI KONSENTRASI AMMONIUM NITRAT DAN WAKTU INKUBASI Wijanarka, Wijanarka; Ferniah, Rejeki Siti; Salamah, Salamah
Bioma : Berkala Ilmiah Biologi Vol. 10, No. 2, Tahun 2008
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (74.151 KB) | DOI: 10.14710/bioma.10.2.58-64

Abstract

Inulinase (E.C.3.2.1.7) merupakan kelompok enzim hidrolase yang mampu menghidrolisis inulin menjadi fruktosa. Produksi fruktosa secara langsung dari inulin oleh enzim inulinase hanya memerlukan satu tahap reaksi enzimatis dan menghasilkan 90% fruktosa sehingga lebih efisien. Optimasi perlu dilakukan untuk meningkatkan produksi inulinase, antara lain dengan penambahan sumber nitrogen dan optimasi waktu inkubasi. Khamir merupakan salah satu mikroba yang dapat memproduksi enzim. Salah satu khamir inulinolitik yang berhasil diisolasi dari umbi dahlia yaitu Pichia alni DUCC-W4. Tujuan penelitian ini adalah mengetahui variasi konsentrasi NH4NO3 dan waktu inkubasi Pichia alni DUCC-W4 dalam memproduksi inulinase pada tepung umbi dahlia. Penelitian ini dilaksanakan di laboratorium mikrobiologi, Jurusan Biologi Fakultas MIPA Universitas Diponegoro. Penentuan aktivitas inulinase dilakukan dengan metode DNS. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) faktorial dengan 2 faktor. Faktor I (P0, P1, P2, dan P3) berupa konsentrasi NH4NO3 yang berbeda yaitu 0,029mM; 0,05 mM; 0,1 mM; 0,15 mM dan faktor II (H1, H2, dan H3) berupa waktu inkubasi (12 jam,18 jam, dan 24 jam). Masing ? masing perlakuan diulang sebanyak 3 kali. Data yang diperoleh dianalisis dengan menggunakan metode ANOVA. Aktivitas inulinase masing ? masing perlakuan pada waktu inkubasi 12 jam yaitu 0,567 U/mL, 0,407 U/mL, 0,304 U/mL, 0,486 U/mL, pada waktu inkubasi18 jam yaitu 0,761 U/mL, 0,644 U/mL, 0,543 U/mL, 0,554 U/mL, sedangkan waktu inkubasi 24 jam yaitu 0,564U/mL, 0,567 U/mL, 0,529U/mL, 0,612 U/mL. Berdasarkan hasil penelitian menunjukkan bahwa penambahan konsentrasi NH4NO3 pada medium produksi dan perbedaan waktu inkubasi tidak meningkatkan aktivitas inulinase Pichia alni DUCC-W4.
AKTIFITAS INHIBITOR ALPHA-GLUKOSIDASE BAKTERI ENDOFIT PR-3 YANG DIISOLASI DARI TANAMAN PARE (MOMORDICA CHARANTIA) Pujiyanto, Sri -; Ferniah, Rejeki Siti
Bioma : Berkala Ilmiah Biologi Vol. 12, No. 1, Tahun 2010
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (140.973 KB) | DOI: 10.14710/bioma, 12, 1, 1-5

Abstract

Some traditional medicinal plants are known to have efficacy as a medicine for diabetes. Active compoundsproduced by a plant can be derived from endophytic microbes that live in these plants. One way diabetes drugs workis to prevent digestion of complex carbohydrates into glucose so that glucose intake is reduced. Alpha-glucosidaseinhibitor is a compound that can prevent the digestion of carbohydrates, especially starch into glucose. This studyaimed to test the inhibitory activity of alpha gluosidase PR-3 isolate, an endophytic bacteria from Momordicacharantia. The results showed that the crude extract (supernatant) from PR-3 has the capability of the enzyme alphaglucosidase inhibition that is equal to 61.2% compared with positive control compound acarbose 1 mg / ml. Theresults also showed that the use of maltose as carbon source produce the highest an alpha glucosidase inhibitor(54,97%), followed by the starch (47.77%), glucose (31.97%), fructose (44.14%) and sucrose (27.7%).
KARAKTERISTIK MORFOLOGI, BIOKIMIA, DAN MOLEKULER ISOLAT KHAMIR IK-2 HASIL ISOLASI DARI JUS BUAH SIRSAK (ANNONA MURICATA L.) Suryaningsih, Vivi; Ferniah, Rejeki Siti; Kusdiyantini, Endang
Jurnal Akademika Biologi Vol. 7 No. 1 Januari 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.943 KB)

Abstract

Yeast was found in foods that contain lots of sugar as fruits. Yeast utilizes simple sugars in food to gain energy. Yeast has a role in the natural fermentation of the fruit that resulting of decay, discoloration, and cause the fruit not durable. The purpose of this research were to isolate the yeast from the soursop fruit and to identify the yeast in morphology, biochemistry, and molecular test based on Internal Transcribed Spacer (ITS). Isolation of  yeast  was  performed by growing on  medium PDA and chloramphenicol. Identification was done through biochemical test by looking at the capabilities in the fermentation of carbohydrate and the abilities to grow on a medium to high osmotic pressure. Molecular identification was done using Internal Transcribed Spacer (ITS) region. The result showed that isolate IK-2 yeast from the soursop fruit juice had a distinctive form round to oval, prominent elevation, the colour creamy white, form a buds, able to ferment glucose and sucrose, but not able to ferment lactose, as well as being able to grow on media with glucose level 50 %. Molecular analysis of the ITS region using ITS1 and ITS4 primers, and phylogenetic analysis using Neighbor Joining. The result of the Basic Local Alignment Tools (BLAST) showed that the isolate had 95% homology with Candida tropicalis. Key Words : Yeast, Soursop (Annona muricata L.), Internal Transcribed Spacer (ITS)