Yuda Heru Fibrianto
Bagian Fisiologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta

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PCS-11 MESENCHYMAL STEM CELL-CONDITIONED MEDIUM IMPROVE THE RECOVERY OF PANCREATIC α AND β CELLS IN TYPE 1 DIABETES MELLITUS Masithoh, Dian Bekti Hadi; Fibrianto, Yuda Heru; Anggita, Marla; Nugroho, Widagdo Sri; Budipitojo, Teguh
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Mesenchymal stem-cell is a pluripotent cells that can be differentiated into many kinds of cells (1) and have abilities to differentiated into mature cell, secreted cytokines, and releasing many kinds of protein functioned in body body regulation (2). However, the therapeutic effect of stem cell arise from their secreted factor such as growth factors, cytokines, chemokines, and metabolits which acts as biologic regulator in the autocrine and paracrine body function (3). Secreted factors of mesenchymal stem cells known as secretome or mesenchimal stem cell conditioned medium (MSC-CM) have therapeutic effect for antiapoptosis, angiogenic, immunomodulatoric, and chemoattraactive activity (4). Mesenchymal stem cell conditioned medium used as the replacement agent for cell therapy because it contains no cells and save for used in different individual (5).Type 1 diabetes mellitus (DMT1) is a disorder characterized by destruction of the insulin-producing pancreatic ?-cell that progressively leads to insulin de?ciency and resultant hyperglycemia. Insulin de?ciency can lead to progressive metabolic derangement, with worsening hyperglycemia, ketoacidosis, starvation, and death (6). Glucagon is a hormone produced by pancreatic ? cells. Glucagon releases by the pancreas to raise the concentration of glucose in the bloodstream when the concentration of glucose in the bloodstream too low (7). Glucagon worked in opposite with insulin, which lowers the extracellular glucose level, while insulin works to increase the extracellular glucose level (8). The aim of this study was to investigate the role of MSC-CM on the structural and functional regeneration of pancreatic ? and ? cells in Wistar rat (Rattus norvegicus) induced with type 1 diabetes mellitus. The datas in this study will completes the information about the effect of MSC-CM on pancreatic cells regeneration, which has been presented in previous study (9).
PCS-10 THE FEATURES OF SEMINIFEROUS TUBULE CELLS IN RAT TESTICULAR DYSFUNCTION INDUCE BY SECRETOME BASED ON CYTOSKELETAL PROTEIN PROFILE Prihatno, Surya Agus; Padeta, Irma; Larasati, Arinda Devi; Sundari, Betty; Hidayati, Annisa; Fibrianto, Yuda Heru; Budipitojo, Teguh
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Secretome is a factor found in the stem cell culture medium. This factor may repair the tissues of the organs that were damaged by various degenerative disorders1. A previous study has reported that secretome derived from the fetal human umbilical cord mesenchymal stem cell (HUC-MSC) may be an effective regenerative agent for ?-cell pancreatic regeneration in Type 1 diabetes mellitus and for skin regeneration in incisional and burn wound healing.The administration of cisplatin as a chemotherapeutic agent is limited because it causes side effects, such as reproductive toxicity and progresses to testicular dysfunction2. As a degenerative disorder, testicular dysfunction is characterized by failure in the synthesis of reproductive hormones and spermatogenesis. Cisplatin-induced testicular cell damage would promote vimentin and cytokeratin localization in the spermatogenic, Sertoli, and Leydig cells.Studies on the use of secretome in promoting the recovery of various degenerative disorders and its effects on the spermatogenesis of rats with cisplatin-induced testicular dysfunction have not been conducted. The aim of this stud was to investigate the effect of secretome derived from HUC-MSC on cisplatin-induced testicular dysfunction in rats.
KADAR MALONDIALDEHID TIKUS DIABETES MELITUS TIPE 2 DENGAN TERAPI EKSTRAK MEDIA PENUMBUH SEL PUNCA MESENKIMAL Fitriana, Ida; Wijayanti, Agustina Dwi; Sari, Puspa Wikan; Satria, Raden Gagak Donny; Setiawan, Dwi Cahyo Budi; Fibrianto, Yuda Heru; Nugroho, Widagdo Sri
Acta VETERINARIA Indonesiana Vol. 5 No. 1 (2017): Januari 2017
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.67 KB) | DOI: 10.29244/avi.5.1.29-36

Abstract

Berbagai penelitian menunjukkan bahwa ekstrak media penumbuh sel punca mesenkimal (EMPSPM), tanpa sel punca itu sendiri, telah ditemukan terdapat berbagai faktor tropik hasil sekresi sel punca mesenkimal di dalam media kultur yang dapat meregenerasi jaringan yang rusak. Penelitian ini bertujuan untuk mengetahui gambaran kadar malondialdehid (MDA) dalam plasma dan ginjal tikus diabetes melitus tipe 2 (DMT2) dengan terapi EMPSPM. Dua puluh lima ekor tikus wistar digunakan dalam penelitian ini dibagi menjadi 5 kelompok yaitu kelompok DMT2 + 0,05 ml/kg BB EMPSPM (0,05); kelompok DMT2 + 0,1 ml/kg BB EMPSPM (0,1); kelompok DMT2 + 0,2 ml/kg BB EMPSPM (0,2), kelompok kontrol DMT2 (DMT2), dan kelompok sehat (KS). Induksi DMT2 dengan menggunakan streptozotosin nikotinamid (STZ-NA). Terapi mulai dilakukan pada hari ke 7 setelah kondisi DM tercapai, diberikan 4 kali dengan selang waktu 7 hari secara intraperitoneal. Data kadar glukosa darah dan MDA dianalisi secara statistik. Hasil penelitian menunjukkan bahwa induksi DMT2 dengan STZ-NA dapat menaikkan kadar glukosa dan MDA dalam darah (P<0,05). Terapi 0,05; 0,1; dan 0,2 ml/kg BB EMPSPM menunjukkan dapat menurunkan kadar glukosa darah, kadar MDA plasma dan ginjal (P<0,05). Kadar 0,2 ml/kg BB EMPSPM menunjukkan aktifitas lebih baik dalam menurunkan kadar glukosa darah dan MDA. Berdasarkan penelitian ini, EMPSPM dapat menurunkan kadar glukosa darah serta kadar MDA dalam darah dan ginjal tikus DMT2.
STUDI SISTEM RESPIRASI DAN KAJIAN MIKROBIOLOGIS LUMBA-LUMBA HIDUNG BOTOL INDO PASIFIK (TURSIOPS ADUNCUS) DARI PERAIRAN LAUT JAWA Mulyani, Guntari Titik; Fibrianto, Yuda Heru; Budipitojo, Teguh; Indrawati, Agustin
Acta VETERINARIA Indonesiana Vol. 2 No. 1 (2014): Januari 2014
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (980.858 KB) | DOI: 10.29244/avi.2.1.7-11

Abstract

Lumba-lumba hidung botol (bottlenose dolphin) adalah spesies lumba-lumba yang paling umum danpaling dikenal orang. Gangguan sistem respirasi pada lumba-lumba sering dijumpai, sementara jenisbakteri yang sering menyerang lumba-lumba dari perairan Laut Jawa belum pernah diteliti. Penelitianini bertujuan untuk mempelajari pemeriksaan klinis sistem respirasi lumba-lumba dan melakukan isolasibakteri serta jamur pada sistem respirasi bottlenose dolphin (Tursiops aduncus) dari Perairan Laut Jawa.Penelitian dilakukan dengan studi pustaka, pemeriksaan klinis serta isolasi sampel sistem respirasi. Swabdeep blowhole dilakukan terhadap sepuluh ekor bottlenose dolphin di PT. Wersut Seguni Indonesia.Sampel dikirim ke laboratorium Mikrobiologi FKH UGM untuk isolasi dan identifikasi terhadap bakteridan jamur. Hasil penelitian menunjukan bahwa pemeriksaan klinis lumba-lumba hanya dapat dilakukansecara inspeksi. Hasil isolasi ditemukan bahwa 5 dari 10 lumba-lumba (50%) positif Staphylococcus aureus,sedangkan jamur tidak ditemukan. Berdasarkan hasil penelitian, disimpulkan bahwa pemeriksaan klinislumba-lumba dapat dilakukan dengan cara inspeksi terhadap blowhole, tingkah laku hewan, adanya?chuff? atau ?honk?, frekuensi respirasi, sosialisasi hewan dan posisi hewan saat berenang. BakteriStaphylococcus aureus dijumpai pada sistem pernafasan lumba-lumba hidung botol dari perairan LautJawa di PT. Wersut Seguni Indonesia.
PENGARUH PENANGKARAN TERHADAP PROFIL ERITROSIT LUMBA-LUMBA HIDUNG BOTOL DARI PERAIRAN LAUT JAWA Mulyani, Guntari Titik; Fibrianto, Yuda Heru; Budipitojo, Teguh
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3636.309 KB) | DOI: 10.22146/jsv.2467

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Di laut Jawa banyak populasi lumba-lumba yang sering tersangkut jala para nelayan saat mencari ikan dilaut. Di Indonesia, lumba lumba hidung botol merupakan mamalia air yang dilindungi. Penelitian ini bertujuanuntuk mengetahui profil eritrosit bottlenose dolphin (lumba-lumba hidung botol) sebelum dan setelah penangkaran. Tujuh ekor lumba-lumba digunakan dalam penelitian ini. Sampel darah diambil melalui vena superfisialis sirip ekor guna pemeriksaan eritrosit, meliputi jumlah, morfologi, kadar hemoglobin dan hematokrit. Pengambilan sampel dilakukan saat lumba-lumba diangkat dari perairan Laut Jawa dan setelah lumba-lumba mengalami penangkaran di PT. WSI, Kendal, Jawa Tengah. Data sebelum dan sesudah penangkaran dibandingkan dan diuji dengan analisis statistik t-test berpasangan pada tingkat signifikasi 95%. Hasil penelitian menunjukkan adanya peningkatan jumlah eritrosit, kadar hemoglobin dan nilai hematokrit secara signifikan. Dari hasil penelitian ini dapat disimpulkan bahwa penangkaran yang dilakukan oleh PT. WSI,Kendal, Jawa Tengah meningkatkan profil eritrosit lumba-lumba hidung botol.Kata kunci : lumba-lumba hidung botol, captivity, profil eritrosit, vena superficial ekor, uji t-berpasangan
PEMASAKAN OOSIT ANJING (CANIS FAMILIARSIS PADA STADIUM ANESTRUS DAN DIESTRUS PADA MEDIA MATURASI YANG DIPERKAYA DENGAN CAIRAN FOLIKEL SAPI 10% DENGAN PENAMBAHAN FOLLICLE STIMULATING HORMONE (FSH) DAN LUTEOTROPIC HORMONE (LH) Fibrianto, Yuda Heru; Hana, Amelia; Pangestiningsih, Tri Wahyu; Wibowo, Pradityo Yoga; Airin, Claude Mona
Jurnal Sain Veteriner Vol 28, No 1 (2010): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4904.308 KB) | DOI: 10.22146/jsv.430

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Effect of Culture Medium Supplementation with b-mercaptoethanol and Amino Acid on Canine Intergeneric Embryo Development with Porcine Oocyte Cytoplasm Recipient Fibrianto, Yuda Heru
Indonesian Journal of Biotechnology Vol 12, No 1 (2007)
Publisher : Universitas Gadjah Mada

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Abstract

The present study investigated the effect of culture medium supplementation with mercaptoethanol ( ME)and amino acid (AA) on canine intergeneric embryo development with porcine oocyte cytoplasm. Porcine cumulusoocyte complexes (COCs) were collected from slaughterhouse and matured in TCM-199 supplemented with 26.2mM NaHCO, 3.05 mM glucose, 0.91 mM sodium pyruvate, 0.57 mM L-cysteine, 75 mg/l kanamycin, 10 ng/ml 3epidermal growth factor, equine chorionic gonadotropin (eCG), 10 IU/ml human chorionic gonadotropin (hCG),and 10% (v/v) porcine follicular fluid (pFF) at 39 °C in a humidified atmosphere of 5% CO for 42-44 h and donor cell 2collected from ear skin afghanhound male dog. After somatic cell nuclear transfer (SCNT), embryo developmentwere examined for cleavage rate and 144 hr for final development after cultured in media. The result shows that,amino acid and mercapoethanol addition in culture medium (NCSU-23) have no effect on embryo development.The development rate of embryo until 16 cell stage in NCSU and NCSU supplement are 4.67% and morula stage are3.73% and 4.67%.Key words : intergeneric clone embryo, canine, ( amino acid (AA)
HIPERGLIKEMIA TIDAK MENYEBABKAN PERUBAHAN BOBOT BADAN DAN JUMLAH NEURON MIENTERIK NITRERGIK USUS HALUS TIKUS WISTAR (RATTUS NORVEGICUS) Hana, Amelia; Astuti, Pudji; Fibrianto, Yuda Heru; Sarmin, Sarmin; Airin, Claude Mona; Tauhid, Tauhid
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (11051.986 KB) | DOI: 10.22146/jsv.38409

Abstract

Digestive dysfunction is a common symptom of diabetes. Diabetes can lead to the loss of enteric neurons as well as nerve dysfunction and gastrointestinal complications. There has been no complete report of mienteric nitrergic of small intestines on hyperglycaemia condition, so the purpose of this study was to assess changes of nitrergic neural profile of small intestines on hyperglycemic Wistar strain rats (Rattus norvegicus). Twelve rats were adapted for 3 days, then they were randomly divided into 3 groups namely group I (K-1, as control), group II (K-2), and group III (K-3). Before treatment all rats were fasted for 12 hours, rats K-1 were induced with 1.0 ml of buffer citrate single peritoneal dose; rats K-2 were induced with 40 mg streptozotocin (STZ)/ kg body weight of single peritoneal dose; and rats K-3 were induced with 45 mg STZ /kg body weight of single peritoneal dose. On the 1st and 5th days after STZ induction, blood was collected through the coccigea vein to measure blood glucose levels and weigh the weight. On the day 5th the rats were uthanated with a high dose of ketamine, dissected and taken small intestines (duodenum, jejunum, and ileum) for staining with Nicotinamide Adenine Dinucleotide Phosphate-diaphorase (NADPH-d). Morphometric changes of nitrergic neurons of the small intestine segments were observed. The results showed that on the 5th day increased blood glucose levels of the K-2 and K-3 groups were significantly higher than K-1 (P <0.01). The weight of three groups did not show any significant improvement difference (P> 0.05). The number of neurons in the duodenum, jejunum and ileum of K-2 group increased higher than that of K-1 and K-3 groups. These results indicate that in hyperglycaemic states, the number of nitrergic neurons increases (K-2 group), and there is a significant decrease in the number of neurons (group K-3) compared with K-1 and K-2 groups (P <0.01). The results of this study concluded, that the STZ induction treatment causes hyperglycemia. Hyperglycemia does not affect to the body weight and the number of  nitrergic neurons of small intestines in Wistar strain rats (Rattus norvegicus).
THE HEART ACTIVITIES IN SOME ENVIRONMENTAL TEMPERATURE ON THE RATS (RATTUS NORVEGICUS) Hana, Amelia; Astuti, Pudji; Fibrianto, Yuda Heru; Sarmin, Sarmin; Airin, Claude Mona
Jurnal Sain Veteriner Vol 30, No 2 (2012): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5758.014 KB) | DOI: 10.22146/jsv.2609

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The aim of this research was to know the heart activity of rats (Rattus norvegicus) at some environmental temperature. Ten rats (Rattus norvegicus), male, 2 months old, the average weight of 200 gr purchased at experiment Animal Care Unit Gadjah Mada University, Yogyakarta. Allf rats were adapted in a cage with food and water ad libitum. Furthermore, each tail rat was measured heart activity is heart rate frequences (beats per minute), mean blood pressure (mmHg), systolic blood pressure (mmHg) and diastolic blood pressure (mmHg) at the environmental temperature of 30o C, 32o C, 34o C, 36o C, 37o C, 38o C, and 40o C  measured with a blood pressure cuff BP-98A at coccygea vein for 3 minutes at each temperature. The measurement was repeated 3 times. The data of the  heart activities obtained were analyzed by using one way analysis of variance. It was concluded that the environmental temperature of 30o C was optimal to the heart activities of the rats, including  heart rate frequences , blood pressure, systolic and diastolic blood pressures.Keywords:    heart rate frequences, blood pressure, systolic and diastolic, environment temperature, Rattus norvegicus
Development of Intergeneric Canine Embryo Using Bovine and Porcine Oocyte as Cytoplasm Recipient Fibrianto, Yuda Heru
Indonesian Journal of Biotechnology Vol 10, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.823 KB) | DOI: 10.22146/ijbiotech.16366

Abstract

This study wa conducted to increase the efficiency of canine embryo production by intergeneric somatic cell nuclear transfer (SCNT) technology. The effect of oocyte recipient for development of intergeneric canine somatic cell cloning embryos were examined. Bovine and porcine cumulus oocyte complexes (COCs) were collected from slaughterhouse ovaries and matured in TCM-199 medium depend on species. Adult dog fibroblasts collected from 3.5 years old Afghandhound dog, and cell between passage 1 and passage 10 used for intergeneric somatic cell cloning using bovine and porcin oocytes as oocyte cytolplasm donor. The result showed that oocytes from bovine and porcine can de-differentiated canine nucleus and no different between bovine and porcine oocyte in fusion and embryo development in vitro. Canine intergeneric cloned embryos developed to morula stages in vitro.