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EFEKTIVITAS BAKTERIOSIN BAKTERI ASAM LAKTAT LOKAL TERHADAP MASA SIMPAN DAN MUTU IKAN PATIN (PANGASIUS SP) Fibriarti, Bernadeta Leni; Septiani, Dea; Sari, Nuria Puspita
Bioma : Jurnal Ilmiah Biologi Vol 8, No 2 (2019): Bioma
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v8i2.4940

Abstract

Penggunaan pengawet alami untuk mempertahankan mutu dan memperpanjang masa simpan ikan merupakan cara yang aman dan tidak berdampak buruk bagi kesehatan. Beberapa bakteri Asam Laktat (BAL) diketahui mampu menghasilkan bakteriosin yang berpotensi digunakan sebagai biopreservatif bahan pangan. Tujuan dari penelitian ini adalah untuk mengetahui kemampuan bakteriosin BAL sebagai biopreservatif untuk mempertahankan mutu dan masa simpan ikan patin (pangasius sp) yang pengaplikasiannya dikombinasikan dengan Natrium klorida (NaCl) and Natrium Asetat (CH3COONa). Metode yang digunakan adalah metode eksperimen dengan tiga perlakuan yaitu kontrol , penambahan BAL, serta kombinasi BAL dengan 3% NaCl dan 3% Na As).Diamati pH dan dihitung jumlah bakteri dengan metode TPC (Total Plate Count).Hasil penelitian menunjukkan perlakuan kombinasi BAL , NaCl dan BAL dengan 3% NaCl dan 3% Na As memiliki efektivitas paling tinggi dalam mempertahankan mutu dan masa simpan ikan patin, mencapai 7 hari lebih lama 3 hari dibandingkan kontrol sedangkan perlakuan kombinasi dengan isolat BAL  5 hariKey words: bakteriosin, bakteri asam laktat, masa simpan ikan, ikan patin (Pangasius sp)
APLIKASI MIKROORGANISME LIGNOSELULOLITIK INDIGENUS ASAL TANAH GAMBUT RIAU DALAM PEMBUATAN KOMPOS DARI LIMBAH TANDAN KOSONG KELAPA SAWIT (Elaeis guineensis Jacq.) Jannah, Wirdatul; Delita Zul, Delita Zul; Fibriarti, Bernadeta Leni
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Palm oil industries usually produce side result such as empty fruit bunch (EFB) wastes. This waste can be employed as a substrate for making compost. Naturally, EFB takes a long time to produce standardized compost as its contains lignin and cellulose components. The purpose of this research was to analyze the ability of indigenous lignocellulolytic microorganisms isolated from peat soil in Riau as the bioactivator. The selected isolates consisting of 4 bacteria (BB_S27, BB_HP42, BB_HP41 and BB_K20) and 2 fungi (LIJ1 and L1J2) were subcultured on Nutrient Broth and Potato Dextrose Broth. Starters were then prepared from a combination of those isolates resulting three types of starter, namely starter I (4 isolates bacteria), starter II (4 isolates bacteria and 1 isolates fungi), starter III (4 isolates bacteria and 2 isolates fungi). As positive controls, starter IV (EM4) and starter V (bioactivator made in USA) were utilized. Starters were prepared by fermentation process during 7 days using seedling media. Composting was done by the windrow composting system utilizing 500 kg EFB as a substrate and inoculated by 50 liters of the starters at the 1st and 7th incubation time. During 35 days composting process, the substrate was enriched by water every 2 days. The best quality compost was produced by K3 treatment (a combination of 4 bacteria) as its characters almost in line with the National Quality Standard (ISO) such as N 1.57%, C/N ratio 23.89, P 0.38%, K 1.61%, blackish brown color, soil smell, unraveled texture, and the highest cell number of bacteria, fungi, and celullolytic microorganisms were with value 1.38x1012 CFU/g, 2.08x107 CFU/g dan 1.35x108 CFU/g, respectively.
APLIKASI MIKROBA LIGNOSELULOLITIK INDIGENUS ASAL TANAH GAMBUT RIAU DALAM PEMBUATAN KOMPOS DARI CAMPURAN TANDAN KOSONG DAN LIMBAH CAIR PABRIK KELAPA SAWIT (Elaeis guineensis Jacq.) Munawarah, Happy Zatul; Zul, Delita; Fibriarti, Bernadeta Leni
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Activity of palm oil industries usually has side result such as empty fruit bunch (EFB) and palm oil mill effluent (POME) wastes. Those wastes still contain organic material that can be utilized as a substrate for compost production. Because of lignin and cellulose components, composting of EFB takes about 60 days to obtain standardized composts. It is known that composting process can be shorterned by adding bioactivators. The purpose of this research was to analyze the ability of indigenous lignocellulolytic microbes isolated from peat soil in Riau as bioactivator in composting of EFB enriched by POME. The selected isolates consisting of 4 bacteria (BB_S27, BB_HP42, BB_HP41 and BB_K20) and 2 fungi (LIJ1 and L1J2) were subcultured on Nutrient Broth and Potato Dextrose Broth. Starters were then made from a combination of the isolates and fermented during 7 days using seedling media. The compost treatments included negative control (seedling media without isolates), positive control by using effective microorganisms (EM) and bioactivator made in USA, and 4 combinations of those isolates resulting 7 treatments. Composting was done by the windrow composting system utilizing 400 kg EFB as a substrate and inoculated by 50 liters of the starters at the 1st and 14th incubation time. During 35 days composting process, the substrate was enriched by 20 liters POME every 2 days. The best quality compost was produced by treatment K3 (a combination of 4 bacteria and 2 fungi) as its characters almost in line with the National Quality Standard (ISO) such as N 2.22%, C/N ratio 14.5, P 0.760%, K 3.44%, blackish brown color, smell like soil and unraveled texture. The highest celullolytic and ligninolytic microbes cell number were also found in compost treated by K3 with value 8.8 x 108 CFU/g and 1.2 X 109 CFU/g, respectively.
SELEKSI ISOLAT JAMUR DALAM MENGHASILKAN HORMON IAA (Indole Acetic Acid) ASAL TANAH GAMBUT DESA RIMBO PANJANG KABUPATEN KAMPAR Astriani, Fenny; Fibriarti, Bernadeta Leni; Zul, Delita
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Indole Acetic Acid (IAA) is one of group of auxin phytohormones and plays a role to improve plant growth. Beside plants, fungi are also known to be able to produce IAA.This study aimed to select the ability of fungal isolates collection of Microbiology Laboratory, Faculty of Math and Natural Sciences University of Riau in IAA production. Fungal isolates were cultured in medium PDB (Potato Dextrosa Broth)enriched by tryptophan as a precursor of IAA. Indole acetic acid produced by fungi was detected by adding Salkowski reagent and determined quantitatively by the use of colorimetric method. The results showed that 47 fungal isolates were able to produce IAA. The highest IAA production was revealed by isolate RPL4-14 (646,75±0,35 ppm) and the lowest was shown by isolate RPL2-x (4,00±3,53 ppm) when they were culturedin PDB medium enriched by tryptophan. The fungal collection did not only produce IAA in the medium containing tryptophan, but also in the medium without tryptophan as shown by isolate RPL3-10, which has IAA concentration i.e 76,50±0,00 ppm and71,00±0,70 ppm, respectively. Isolates which produced the highest IAA concentration are identified as Penicillium sp.
SELEKSI KEMAMPUAN BAKTERI PELARUT FOSFAT ASAL BUKIT BATU-RIAU DALAM MENGHASILKAN ASAM SIANIDA Aprillia, Pristiana; Zul, Delita; Fibriarti, Bernadeta Leni
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 1 (2014): Wisuda Februari 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Phosphate solubilizing bacteria (PSB) is beneficial bacteria which are not only capablein solubilizing organic phosphate into inorganic phosphate, but also producing cyanideacid (HCN). HCN plays a role in controling weed growth. The objective of this researchwas to screen the potency of indigenous PSB isolated from peat soil in producting HCN.The collections of PSB were subcultured in King’B broth. The ability of 152 isolates onHCN production were tested qualitatively using filter paper soaked with picric acid andNa 2 CO 3 . The result indicated that as many as 26 isolates (17.1%) were able to releasevarious levels of HCN production based on the appearing of brown, dark brown, orreddish brown colour on the filter paper.
STUDI PERTUMBUHAN DAN DEGRADASI FENOL OLEH KULTUR TUNGGAL AKTINOMISETES DARI TANAH GAMBUT Masni, Tiara Elsita; Linda, Tetty Marta; Fibriarti, Bernadeta Leni
Al-Kauniyah: Jurnal Biologi Vol 13, No 1 (2020): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2866.46 KB) | DOI: 10.15408/kauniyah.v13i1.12854

Abstract

AbstrakFenol adalah senyawa organik yang bersifat toksik dan larut dalam air, sehingga mudah menimbulkan pencemaran pada perairan dan menurunkan kualitas air. Penelitian ini bertujuan untuk melihat potensi tiga isolat aktinomisetes asal tanah gambut Riau dalam Minimal Salt Medium yang mengandung fenol pada konsentrasi 0 ppm, 200 ppm, 400 ppm, dan 600 ppm serta mengetahui kemampuan aktinomisetes dalam mendegradasi fenol pada konsentrasi 600 ppm menggunakan metode folin ciocalteau. Potensi pertumbuhan isolat L121, L18, L11 menunjukkan total populasi tidak berbeda nyata dengan penambahan 400 ppm dan 600 ppm fenol, tetapi berbeda nyata terhadap 0 ppm dan 200 ppm fenol. Potensi pertumbuhan tertinggi terdapat pada isolat L121 dan terendah pada isolat L11. Kemampuan degradasi  fenol oleh  masing-masing isolat adalah  L121 sebesar 570,80 ppm (95%),  L18 sebesar 218,85 ppm (36%)  dan L11 sebesar  97,21 ppm (16%)  dari konsentrasi fenol awal 600 ppm pada Minimal Salt Medium. Isolat aktinomisetes ini berpotensi dikembangkan untuk penanggulangan pencemaran di lingkungan.Abstract Phenol is an organic compound is toxic and easily soluble in water so easy to cause pollution in a waters such as water quality degradation. The aim of this research is to see the potential of three isolates of actinomycetes from Riau peat soil in Minimal Salt Medium containing phenol concentration 0 ppm, 200 ppm, 400 ppm and 600 ppm and to know the ability of actinomycetes in degradation of phenol at the concentration of 600 ppm using folin ciocalteu. The growth potential of L121, L18, L11 isolates showed the total population was not significantly different with the addition of 400 ppm and 600 ppm of phenol but significantly different from 0 ppm and 200 ppm of phenol. The highest growth potential was found in L121 isolate and lowest in L11 isolate. The degradation ability of phenols by each isolate was L121 570.80 ppm (95%), L18 218.85 ppm (36%) and L11 was able to degrade phenol 97.21 ppm (16%) from the initial phenol concentration of 600 ppm at Minimum Salt Medium.These actinomycetes have the potential to be developed for the overcome of pollution in the environment.
Seleksi Potensi Bakteri Probiotik Indigenus Riau sebagai Agen Biopreservatif Bahan Pangan WULANDARI, NUR ASYIAH; FIBRIARTI, BERNADETA LENI; ROZA, RODESIA MUSTIKA
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

The use of synthetic preservatives is not safe for consumption and very dangerous for health.Lactic acid bacteria (LAB) is a probiotic bacterium that can produce bacteriocins asbiopreservatives agents. Bacteriocins have been widely applied as a food preservative, but itscommercial availability is still low and very expensive. This study was conducted to test the abilityof 23 isolates of local probiotic bacteria in Riau to produce bacteriocins. LAB total population wascalculated by Total Plate Count (TPC) method. LAB inhibition test against indicator bacteriaStaphylococcus aureus FNCC 0047 was conducted using well diffusion method. Bacteriocinsantimicrobial confirmatory test was performed at 100OC for 15 minutes, 30 minutes, and 60minutes. The result showed that the highest LAB population was PSG4 (22.45 x 106 cfu/ml) andthe lowest was SLK2 (4.75 x 106 cfu/ml). The greatest inhibitory activity against indicator bacteriaS. aureus FNCC 0047 was found in PSG2 (15.1 mm) and the smallest was in SLK2 (10.75 mm)with the activity of bacteriocins 2575 mm2/ml and 792.6 mm2/ml respectively. The bacteriocinsconfirmatory test showed clear zone of isolates JN5, PSG2, and SLK2 with the largest inhibitionzone was during heating for 30 minutes and 60 minutes (19.2 mm, 19.5 mm, and 16.8 mm) whilethe smallest was for 60 minutes and 15 minutes (12.8 mm, 15.6mm, and 14.7 mm).Keywords: Probiotic bacteria, biopreservatives, bacteriocins, Staphylococcus aureus FNCC 0047.
FORMULASI PUPUK HAYATI SERBUK MENGGUNAKAN BAKTERI PELARUT FOSFAT INDIGENUS ASAL TANAH GAMBUT RIAU DALAM BERBAGAI BAHAN PEMBAWA Haryanti, Dewi; Zul, Delita; Fibriarti, Bernadeta Leni
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Peat soil has been known for its low content in phosphor (P). On the other hand, P solubilizing process is naturally slow. Inoculation of phosphate solubilizing bacteria (PSB) could increase this process. Therefore, the exploration of bacteria with such potential for use as biofertilizer agents is needed. Quality of biofertilizer depends on the viability and amount of bacteria contained, it is also influenced by type of carrier material. This research was aimed to find the appropriate carrier materials in production of biofertilizer which were contained PSB agent and to determine the quality of biofertilizer produced at a certain storage time. As many as 4 selected PSB isolates (BB_UB6, BB_K9, BB_K2, and BB_HS13) were used to produce 3 starter combinations. The starters were prepared by growing the isolates in Pikovskaya’s media. Biofertilizer was produced by inoculating each starter into the peat and wood charcoal as carrier material and was fermented for 4 days. Biofertilizer quality was determined by calculating the PSB cells number during 0, 30, 60, and 90 days storage time and the degree of biofertilizer acidity. The cell numbers of starter I, II, and III ranged from 8.2.1010-2.9.1011 CFU/g, 5.3.1010-2.9.1011 CFU/g, and 8.0.1010-2.9.1011 CFU/g, respectively in a period of storage 0-90 days. The highest cell number at the end of storage was found from biofertilizer produced by using peat, as carrier material which was kept at 40C (1,3.1011 CFU/g, starter I), (8.6.1010 CFU/g, starter II), and (9.6.1010 CFU/g, starter III). Biofertilizer acidity was relatively neutral, 6.38. Based on the results obtained, peat was revealed as a good carrier material and the quality of biofertilizers which were produced was still good.
Seleksi Isolat Aktinomisetes Asal Tanah Gambut Desa Rimbo Panjang Kabupaten Kampar Dalam Menghasilkan Hormon IAA (Indole Acetic Acid) MAWARTI, INDAH; FIBRIARTI, BERNADETA LENI; ZUL, DELITA; ROZA, RODESIA MUSTIKA; MARTINA, ATRIA; LINDA, TETTY MARTA
Jurnal Riau Biologia Vol 2, No 1 (2017)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Hormon IAA (Indole Acetic Acid) termasuk fitohormon golongan auksin yang berperan sebagai zat pemacu pertumbuhan tanaman. Selain tumbuhan, mikroba juga diketahui mampu menghasilkan IAA. Penelitian ini bertujuan untuk menyeleksi kemampuan koleksi isolat aktinomisetes Laboratorium Mikrobiologi FMIPA UR dalam menghasilkan IAA. Uji produksi IAA oleh aktinomisetes dilakukan dengan penambahan reagen Salkowski menggunakan metode kolorimetri dalam medium SCB (Starch Casein Broth) yang diperkaya dengan triptofan sebagai prekursor dan tanpa triptofan. Hasil penelitan ini diperoleh sebanyak 50 dari 85 isolat aktinomisetes yang memiliki kemampuan dalam menghasilkan hormon IAA. Produksi IAA tertinggi dihasilkan oleh RB5S78 sebesar 35,97 ppm dan konsentrasi terendah dihasilkan oleh RB4S67 sebesar 0,24 ppm dalam medium SCB yang diperkaya triptofan. Pada medium tanpa triptofan hasil tertinggi diperoleh isolat aktinomisetes RB5S78 dengan konsentrasi sebesar 15,28 ppm dan konsentrasi terendah dihasilkan oleh RB4S65 sebesar 0,16 ppm. Isolat aktinomisetes yang menghasilkan IAA dengan kriteria tinggi yang diperoleh termasuk dalam genus Streptomyces dan Rhodococcus.
FORMULASI BIOFERTILIZER CAIR MENGGUNAKAN BAKTERI PELARUT FOSFAT INDIGENUS ASAL TANAH GAMBUT RIAU Yelti, Suci Novri; Zul, Delita; Fibriarti, Bernadeta Leni
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Inefficient of P-uptake by plants during the application of chemical phosphorus (P) fertilizer results P-leaching to the aquatic environment. Therefore, the use of phosphate solubilizing bacteria (PSB) to enhance solubilization of P is necessary. The aims of this study were to find the best formulation in producing liquid biofertilizer which contained PSB and to analyze the storage time of liquid biofertilizer which were produced. As many as 4 selected PSB isolates (BB_UB6, BB_K9, BB_K2 and BB_HS13) were used to produce 3 combination starters. Liquid biofertilizers were produced by fermentation using three types of formulation, namely Pikovskaya's medium, coconut water enriched with 2% molasses, and tofu waste water. The quality of liquid biofertilizer was determined by calculating the PSB cells number during 0, 30, and 60 days of storage time and by measuring the liquid biofertilizer acidity. Liquid biofertilizers produced were kept at room and refrigerator temperature. The results showed that the PSB cell numbers of starter I, II and III were higher in liquid biofertilizer that was formulated with coconut water which containing 2% of molasses until 60 days of storage time. The cell numbers of PSB ranged from 7,0×1010 - 2,82×1011 CFU/ml. In general, the PSB cell number was relatively stable when liquid biofertilizer was kept at room temperature. Based on this results, it can be concluded that the best formulation to produce liquid biofertilizer was the coconut water enriched with 2% molasses.