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PROFIL DAN SENSITIVITAS PROTEIN ALERGEN IKAN TONGKOL (THUNNUS ALBACARES) SEBAGAI REAGEN SKIN PRICK TEST (SPT) Chalid, Sri Yadial; Syah, Dahrul; Giriwono, Puspo Edi; Rungkat, Fransiska; Zakaria, Zakaria
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 5, No. 1, May 2019
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Syarif Hidayatullah State Islamic Uni

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (679.985 KB) | DOI: 10.15408/jkv.v5i1.9678

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Ikan tongkol merupakan salah satu produk laut penyebab alergi makanan. Gejala klinis reaksi alergi makanan adalah gatal, bentol, bengkak, sesak nafas, batuk, dan yang terparah adalah syok anafilaksis. Pengobatan dan pencegahan alergi makanan yang terbaik adalah menghindari konsumsi penyebab alergi. Menghindari konsumsi suatu makanan sebaiknya berdasarkan uji alergi seperti Skin Prick Test (SPT). SPT dilakukan menggunakan reagen SPT yang dicukitkan pada lapisan epidermis kulit lengan bagian volar. Reagen alergen pada penelitian ini merupakan protein ikan tongkol yang berasal dari laut Indonesia dan diekstrak dengan buffer fosfat, Profil ekstrak protein menggunakan elektroforesis dan immunoblotting untuk menentukan protein allergen. Sebanyak 15 pita protein terdeteksi pada ekstrak ikan tongkol yaitu protein dengan berat molekul antara 17 sampai 152 kDa. Potensi alergenik terdapat pada pita protein dengan berat molekul antara 12 sampai 50 kDa. Reagen SPT ikan tongkol mempunyai sensitivitas sebesar 81.8% dan spesifitas 100% sehingga disimpulkan bahwa reagen ekstrak ikan tongkol dapat digunakan sebagai reagen skin prick test Kata kunci: Alergi, protein, tongkol, gejala klinis, skin prick test   Tuna fish is one of the marine products that can cause allergic. Clinical symptoms of allergic are a bump, swelling, shortness of breath, coughing and anaphylactic shock is the worst symptom. The best medication and treatment is avoiding the consumption of food that could cause allergy. Avoiding consumption of food should be based on an allergy test such as a Skin Prick Test (SPT). SPT using reagent which is applied on the skin of the forearm between the wrist and elbow. The reagent for SPT is a protein solution of tuna which was extracted by phosphate buffer then protein profile was detected using electrophoresis. Immunoblotting was done to determine the molecular weight of the allergen protein. Fifteen protein bands were detected on tuna fish extract; the molecular weight of the protein was obtained between 17 to 152 kDa. Protein allergenic are molecules that have protein bands with a molecular weight between 12 and 50 kDa. Tuna fish extract solution for SPT had a sensitivity is 81.8%, and specificity is 100%, it was concluded that tuna fish reagent could be used as SPT reagent. Keywords: Allergen, protein, tuna fish, skin prick test.
PARAMETER KINETIKA INAKTIVASI TERMAL DAN ISOLASI STAPHYLOCOCCUS AUREUS PADA MINUMAN DARI GEL CINCAU HIJAU DAN ROSELA Purnomo, Eko Hari; Giriwono, Puspo Edi; Indrasti, Dias; Firlieyanti, Antung Sima; Kinasih, Andini Giwang
Jurnal Teknologi dan Industri Pangan Vol. 26 No. 1 (2015): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (192.466 KB) | DOI: 10.6066/jtip.2015.26.1.124

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Information about heat resistance (D and z values) of target bacteria is needed for the thermal process design on drink from Green Grass Jelly (Premna oblongifolia Merr.) and Roselle (Hibiscus sabdariffa L.), so it can guarantee quality and safety of the product. The objectives of this research were to isolate Staphylococcus aureus from commercial green grass jelly and to determine the D and z values of Staphylococcus aureus (from commercial product and standard clinical isolate ATCC 25923) on heating menstruum of green grass jelly and roselle. Isolation of S. aureus was done by inoculation in selective medium, D and z values of Staphylococcus aureus were assessed by heating at constant temperatures of 57, 53, 49, and 45°C during the time interval 2.5, 5, 10, and 15 minutes. The results showed that one isolate (Isolate A) gave positive response of Staphylococcus aureus in isolation tests and had similar percentage with the reference culture of 41.8% using the API Staph Kit. Heat resistance of Staphylococcus aureus (represented as D value) isolated from green grass jelly at constant heating temperature of D45, D49, D53 and D57 were 32.3, 17.9, 4.6, and 1.5 minutes. On the other hand, D value of isolates ATCC 25923 (standard clinical isolate) at constant heating temperature of D45, D49, D53 and D57 were 18.5, 6.8, 2.9, and 1.4 minutes. The z value of isolates A and ATCC 25923 were 8.8°C and 10.8°C. Smaller z value of isolate A showed that pasteurization process can be accelerated and optimized with increasing the temperature slightly but has the same lethality effect.
PENGARUH MINUMAN BEROKSIGEN TERHADAP SISTEM IMUN, KADAR MALONALDEHIDA DAN PERFORMA RESPONDEN MAHASISWA OLAHRAGAWAN [EFFECT OF OXYGENATED WATER ON IMMUNE SYSTEM, MALONALDEHYDE CONTENT AND STUDENT-ATHLETE PERFORMANCE] Refdi, Cesar Welya; Zakaria, Fransiska Rungkat; Giriwono, Puspo Edi
Jurnal Teknologi dan Industri Pangan Vol. 25 No. 1 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (489.684 KB) | DOI: 10.6066/jtip.2014.25.1.90

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PENGARUH MINUMAN BEROKSIGEN TERHADAP  SISTEM IMUN, KADAR MALONALDEHIDA DAN PERFORMA RESPONDEN MAHASISWA OLAHRAGAWAN[Effect of Oxygenated Water on Immune System, Malonaldehyde Content and Student-Athlete Performance]Cesar Welya Refdi, Fransiska Rungkat Zakaria* dan Puspo Edi GiriwonoDepartemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Bogor Diterima 23 Agustus 2013 / Disetujui 01 Juli 2014ABSTRACT   Exercise requires more oxygen input and cannot be fully obtained through breathing. The body has a homeostatic system, when more ATP is needed, the more oxygen is required by the body. Limited supply of oxygen such as those happens in athlete?s body automatically switches the respiration from aerobic into anaerobic. This situation results in building up of lactic acid which in turn lowers the energy for cellular metabolism as well asenergy for exercise. The purpose of this study was to assess the effects of short-term and long-term drinking of oxygenated water on lactic acid and energy expenditure (EE) in exercise performance; Cluster of Differentiation-4 (CD4), CD8, CD56, Interleukin-6 (IL-6) contents; and level of malonaldehyde. During short-term intervention, 12 male student volunteers drank oxygenated (50, 80 and 130 ppm) water and normal mineral water, 15 minutes before treadmill and after treadmill (10 Kmh). The lactic acid concentration was measured before and after treadmill, and 5 minutes after the reconsumption of the oxygenated water. During long-term intervention, 17 male student volunteers drank oxygenated (100 ppm) water twice a day for 21 days. The blood plasma and lymphocyte sampled before and after intervention were analyzed for the same parameters. The results showed that oxygenated water had no effect on lactic acid accumulations and EE. Similarlyt he long-term study showed that oxygenated water did not affect CD4 and CD8, had no harmful effect on IL-6 and malonaldehyde, yet it significantly increased the CD56 content.  
KOMPONEN BIOAKTIF KOPI BERPOTENSI SEBAGAI ANTIDIABETES / THE POTENCY OF BIOACTIVE COMPOUNDS OF COFFEE AS ANTIDIABETIS Tarigan, Elsera Br; Herawati, Dian; Giriwono, Puspo Edi
Perspektif Vol 19, No 1 (2020): Juni 2020
Publisher : Puslitbang Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/psp.v19n1.2020.41-52

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Recently, the popularity of coffee is gaining popularity. The researcher found that the benefit of coffee was not refreshing only but also improved the quality of health. These effectsexistdue to the natural bioactive compounds found in the coffee. The bioactive compounds of coffee have activity as an antioxidant, anti-inflammation, anti-microbe, and recently as antidiabetic. The major compounds found in coffee were chlorogenic acid, trigonelline, diterpene, and Maillard reaction product (exp.melanoidin). The objective of this study was to explore the bioactive compounds of coffee and the potency antidiabetic, conducted by in-vitro, in-vivo, clinically, and epidemiology intergrately. The in-vitro analysis shown thatcoffee had activity asan inhibitor a-glucosidase, the compounds were chlorogenic acids. In the in-vivo study,coffee brewwas able to reduce blood glucose concentration of a rat model of type-2 diabetes by increasing insulin sensitivity. Caffeine and chlorogenic acids probably had an antagonist effect on glucose response. At the early stage of a clinical study, blood glucose concentration tend too increasedacutely and gradually reduces along with insulin sensitivity higher. A chlorogenic acid had a potency to decrease blood glucose concentration byseveral mechanisms such as ?-glucosidase inhibitory and raise insulin sensitivity. Furthermore, epidemiology studied shown that the efficacy of coffee consumption in the long-termwas able to reduce the risk of diabetes type 2. The effectiveness of coffee as antidiabetic depends on some factors such as gender and variation of coffee such asvariety, brewing technique, and frequency consumption of coffee.ABSTRAK Konsumen kopi saat ini makin meningkat, kepopulerannya ditandai dengan industri hilir kopi yang merebak di tengah-tengah masyarakat. Konsumsi kopi selain memberikan efek menyegarkan juga memiliki manfaat dalam meningkatkan taraf  kesehatan konsumennya. Komponen bioaktif pada kopi memiliki aktivitas seperti antioksidan, antiinflamasi, antimikroba dan antidiabetes. Kandungan biokatif kopi yang berperan dalam aktivitas tersebut adalah asam klorogenat, trigonelin, diterpen dan produk reaksi Maillard (cth.melanoidin). Tujuan dari tulisan ini adalah menggali senyawa bioaktif yang terdapat pada kopi dan potensinya sebagai antidiabetes secara terpadu baik secara in-vitro, in-vivo, klinis dan epidemiologi. Berdasarkan penelitian secarain?vitrobahwa komponen bioaktif kopi yang berperan dalam menghambat aktivitas a-glukosidase adalah asam klorogenat. Secara in-vivobahwa seduhan kopi yang dikonsumsi oleh tikus penderita diabetes menghasilkan kadar glukosa darah yang menurun karena peningkatan sensitivitas insulin. Efek kafein kemungkinanberlawanan dengan asam klorogenat terhadap glukosa darah. Pada awal pengujian secara klinis kadar glukosa darah akan meningkat secara akut dan kemudian menurun seiring meningkatnya efek asam klorogenat. Asam klorogenat akan berperan dalam menghambat transportasi glukosa dan meningkatkan sensitivitas insulin. Penelitian secara epidemiologi menunjukkan bahwa konsumsi kopi dalam jangka waktu yang lebih lama dapat menurunkan resiko penyakit diabetes mellitus tipe 2. Persentase penurunan penyakit diabetes melitus dipengaruhi oleh faktor gender dan variasi kopi seperti jenis, teknik menyeduh dan frekuensi konsumsi kopi.
AKTIVITAS ANTIOKSIDAN DAN PENGHAMBATAN α-GLUKOSIDASE OLEH EKSTRAK ETANOL BAKTERI ASAM LAKTAT INDIGENUS Farida, Eko; Jenie, Betty Sri Laksmi; Nuraida, Lilis; Giriwono, Puspo Edi
Jurnal Teknologi dan Industri Pangan Vol. 30 No. 1 (2019): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (461.223 KB) | DOI: 10.6066/jtip.2019.30.1.56

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Lactic acid bacteria (LAB) are one of Indonesia's biodiversity which can be beneficial for food and health purposes. Some of LAB are potential probiotics with specific functional properties, such as antidiabetes. This study evaluated the effect of ethanol extracts of twelve indigenous LAB in inhibiting ?-glucosidase enzyme and their antioxidant activities. Assay for the ?-glucosidase inhibition was performed on LAB ethanol extract using spectrophotometric method at ?=410 nm, while the antioxidant activity was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method at ?=517 nm. The results showed that ?-glucosidase inhibition was significantly different between the isolates (P<0.01). Lactobacillus fermentum S21209 had the highest ?-glucosidase inhibition activity, which was significantly different from Lactobacillus plantarum MB427, Lactobacillus plantarum Pi28a, Lactobacillus delbrueckii W24802 and Lactobacillus plantarum 2 W22409. Evaluation of the antioxidant activity also showed significant difference between the isolates (P<0.01). Lactobacillus plantarum BSL had the highest antioxidant activity (92.81±1.36%), which was not significantly different from vitamin C as a control. This preliminary study reported that twelve indigenous LAB could be used as potential antidiabetic probiotics, although the responsible compounds are not known.
SENYAWA BIOAKTIF TORBANGUN (Plectranthus amboinicus (Lour.) SPRENG) FRAKSI KLOROFORM INDUKSI APOPTOSIS PADA KANKER PAYUDARA (SEL MCF-7) IN VITRO Yulianto, Wahid; Andarwulan, Nuri; Giriwono, Puspo Edi; Pamungkas, Joko
Majalah Obat Tradisional Vol 22, No 1 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (355.642 KB) | DOI: 10.22146/tradmedj.24312

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Tanaman Torbangun (Plectranthus amboinicus (Lour.) Spreng) telah dimanfaatkan secara tradisional pada berbagai negara di daerah tropis untuk menyembuhkan berbagai penyakit. Penelitian ini bertujuan untuk mengidentifikasi komponen aktif pada fraksi kloroform dari ekstrak tanaman torbangun tersebut yang memiliki efek terhadap ekspresi gen-gen yang berkaitan dengan apoptosis pada sel kanker payudara MCF-7. Apoptosis diamati secara morfologis menggunakan metode pewarnaan inti sel Hoechst. Ekspresi gen dianalisa menggunakan Real-Time PCR sedangkan komponen bioaktif diidentifikasi menggunakan LC-MS. Hasil pengamatan morfologi secara jelas memperlihatkan terjadinya apoptosis pada sel setelah diberi perlakuan ekstrak tanaman. Terjadi peningkatan ekspresi gen anti-apoptosis Bcl-2 namun peningkatan tersebut tidak mampu mencegah terjadinya apoptosis pada sel. Ekspresi gen p53 dan p21 meningkat secara signifikan setelah diberi perlakuan fraksi kloroform. Ekspresi gen caspase 9, caspase 7 dan caspase 1 meningkat dengan level peningkatan yang dipengaruhi oleh konsentrasi yang diberikan. Sebagian besar komponen yang teridentifikasi tergolong dalam kelompok diterpenoid yang kemungkinan berkontribusi terhadap bioaktifitas fraksi kloroform dalam menginduksi apoptosis pada sel kanker MCF-7.
Enterococcus faecium 1.15 Isolated from Bakasam Showed Milk Clotting Activity Putranto, Wendry Setiyadi; Suradi, Kusmajadi; Chairunnisa, Hartati; Mustopa, Apon Zaenal; Giriwono, Puspo Edi; Kusumaningrum, Harsi Dewantari; Suhartono, Maggy Thenawidjaja
ANNALES BOGORIENSES Vol 21, No 1 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (499.913 KB) | DOI: 10.14203/ab.v21i1.293

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The Lactic Acid Bacteria with Milk Clotting Activity (MCA) were isolated from Bakasam, an Indonesian traditional fermented meat. The isolate screening was carried out using modified method of Skim Milk Agar and Milk Clotting Activity Test, and the isolate was then identified using 16S rRNA. We found 4 isolates that showed MCA of 18-20 SU/ml. Identification using 16S rRNA indicated that the isolate ALG.1.15 was 99% (FR3-F primer) and 99% (FR3-R primer) identic with Enterococcus faecium. The isolate potentially produced renin-like protease to subtitute renin from veal.  
The Development of Protein Extract from Local Indonesian Shrimp (Penaeus merquiensis) for Food Allergies Reagent Using Skin Prick Test Method CHALID, SRI YADIAL; SYAH, DAHRUL; GIRIWONO, PUSPO EDI; ZAKARIA, FRANSISKA RUNGKAT
JURNAL ILMU KEFARMASIAN INDONESIA Vol 13 No 2 (2015): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (972.261 KB)

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Jerbung is a kind of shrimp which widely spread in Indonesian marine that is a major allergen from seafood origin. Until now, the best treatment for food allergies is avoid from all kinds of food allergens. Avoiding certain foods should be established based on allergy tests such as skin prick test (SPT) and double-blind placebo-controlled food challenges. SPT is the most widely used test for detecting IgE-mediated food allergies. The main ingredient of SPT are protein extract which is called the reagent. Jerbung protein was extracted from part of meat by buffer phosphat saline (PBS) then analyzed by using SDS-PAGE and protein concentration was analyzed by Bradford assay. Protein extract was formulated to SPT reagent based on standarization of the European Pharmacopoeia Monograph on Allergen Products 7 (2010:1063). Skin prick test reagent was applied on 24 adult participants who were food allergies and 16 adults individuals of non food allergies. Sera of subjects were collected to measuretotal IgE and specific IgE-binding. The sensitivity of protein extract was 95% and negative error of 5%. Whereas specificity was 94% with a positive error was 6%. Immunobloting was also performed on 10 sera subjects. Nine sera subjects of these shrimp allergic participants showed the specific binding to allergen of jerbung shrimp with the range of molecular weight of 28-63 kDa.
Role of Nutrition and Health Claims on Growing Up Milk Product as Purchasing Decision Determination RTR, Ayu Puspitalena; Palupi, Nurheni Sri; Giriwono, Puspo Edi
Jurnal Mutu Pangan (Indonesian Journal of Food Quality) Vol 2, No 2 (2015)
Publisher : Jurnal Mutu Pangan (Indonesian Journal of Food Quality)

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Abstract

Abstract. . This moment,it has not been known the role of nutrition and health claims as purchasing decision determination. Objective of the research is to study the role of nutrition and health claims on the labels of growing up milk as purchasing decision and to analyze corellation of respondent's characteristics which have influence. Study was conducted by survey, data were analyzed descriptively and statistically. Label of growing up milk for children 1-3 years old read by 70% respondent and 30% occasionally, and 23% of respondents do not read nutrition and health claims. Nutritional and health claims have a role as a determinant of purchasing decisions for consumers of 24%. As for the 76% purchase decision is influenced by other factors such as suitable with a child, nutrient content, good taste or price. The role of nutritional and health claims as a determinant of purchase decisions are influenced by factors of education, family expenses per month and expenditure for milk per month. As for the other factors that include age, occupation, number of children, place of purchase, and frequency of purchases did not affect the role of nutrition and health claims as a determinant of purchase. Keywords: Label, growing up milk, nutrition and health claims, purchasing decisions Abstrak. Saat ini belum diketahui peranan klaim gizi dan kesehatan sebagai penentu keputusan pembelian. Penelitian bertujuan mengkaji peranan klaim gizi dan kesehatan pada label produk susu pertumbuhan terhadap keputusan pembelian dan menganalisis hubungan karakteristik responden yang berpengaruh. Penelitian dilakukan dengan metode survei, data dianalisis secara deskriptif dan statistik. Sebanyak 70% responden membaca label produk susu pertumbuhan untuk anak usia 1-3 tahun dan 30% kadang-kadang, serta 23% responden tidak membaca klaim gizi dan kesehatan. Klaim gizi dan kesehatan mempunyai peranan sebagai penentu keputusan pembelian bagi konsumen yaitu sebesar 24%. Sebesar 76% keputusan pembelian dipengaruhi oleh faktor lainnya seperti cocok dengan anak, kandungan gizi, rasa yang enak ataupun harganya. Peranan klaim gizi dan kesehatan sebagai penentu keputusan pembelian dipengaruhi oleh faktor pendidikan, pengeluaran keluarga per bulan dan pengeluaran untuk susu per bulan. Faktor lain yang meliputi usia, pekerjaan, jumlah anak, lokasi pembelian, dan frekuensi pembelian tidak mempengaruhi terhadap peran klaim gizi dan kesehatan sebagai penentu pembelian. Kata kunci: Label, susu pertumbuhan, klaim gizi dan kesehatan, keputusan pembelian Aplikasi Praktis: Klaim gizi dan kesehatan pada label susu pertumbuhan untuk anak usia 1-3 tahun berperan sebagai penentu keputusan pembelian. Namun demikian belum semua responden membaca label serta klaim gizi dan kesehatan karena kurang dapat dipahami. Hasil ini dapat digunakan sebagai bahan pertimbangan bagi badan regulasi untuk mengatur klaim gizi dan kesehatan dalam bahasa yang lebih dapat dimengerti oleh masyarakat. Bagi produsen bermanfaat untuk dapat menentukan klaim gizi dan kesehatan yang tepat pada label sehingga berperan efektif sebagai pertimbangan dalam memilih produk. http://jurnalmutupangan.com/index.php?link=view&id=JMP-02-17-037
Prevalensi Penyakit Kanker di Indonesia Berdasarkan Pola Konsumsi Pangan dan Gaya Hidup (Cancer prevalence in Indonesia Based on Food Consumption Patterns and Lifestyle) Aliwikarta, Kuswanto; Palupi, Nurheni Sri; Giriwono, Puspo Edi
Jurnal Mutu Pangan (Indonesian Journal of Food Quality) Vol 3, No 1 (2016)
Publisher : Jurnal Mutu Pangan (Indonesian Journal of Food Quality)

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Consumption of processed meat is associated to cancer risk increasing. This study used secondary data from RISKESDAS 2013 with a case-control study design, is aimed to analyze the cancer prevelance in Indonesia based on food consumption patterns and lifestyles. The case population is 90 people suffering from cancer and 90 people with no cancer randomly selected as control. Respondent profiles were analyzed by univariate analysis. The relationship of cancer risk factors were analyzed by bivariate analysis and determination of risk factors using multivariate analysis. Sensitivity and specificity analyses showed the cancer vulnerable age is ≥46 years. Processed meat consumption is associated significantly (p=0.000; OR=1.25) in the cancer prevelance. Respondents frequently eat processed meat risk is predicted 1.25 times compared to those not taking. Vegetable consumption (p=0.046; OR=0.509) and fruit consumption (p=0.022; OR=0.365) related significantly to cancer. Vegetables consumption reduce the risk by 0509 times, and fruit consumption reduce the risk by 0365 times. Lifestyle risk factors (physical activity and smoking) significant (p=0.000) to cancer. Multivariate analysis results showed the age, gen- der and processed meat consumption is associated significantly (p=0.000) to cancer.