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PARAMETER KINETIKA INAKTIVASI TERMAL DAN ISOLASI STAPHYLOCOCCUS AUREUS PADA MINUMAN DARI GEL CINCAU HIJAU DAN ROSELA Purnomo, Eko Hari; Giriwono, Puspo Edi; Indrasti, Dias; Firlieyanti, Antung Sima; Kinasih, Andini Giwang
Jurnal Teknologi dan Industri Pangan Vol. 26 No. 1 (2015): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (192.466 KB) | DOI: 10.6066/jtip.2015.26.1.124

Abstract

Information about heat resistance (D and z values) of target bacteria is needed for the thermal process design on drink from Green Grass Jelly (Premna oblongifolia Merr.) and Roselle (Hibiscus sabdariffa L.), so it can guarantee quality and safety of the product. The objectives of this research were to isolate Staphylococcus aureus from commercial green grass jelly and to determine the D and z values of Staphylococcus aureus (from commercial product and standard clinical isolate ATCC 25923) on heating menstruum of green grass jelly and roselle. Isolation of S. aureus was done by inoculation in selective medium, D and z values of Staphylococcus aureus were assessed by heating at constant temperatures of 57, 53, 49, and 45°C during the time interval 2.5, 5, 10, and 15 minutes. The results showed that one isolate (Isolate A) gave positive response of Staphylococcus aureus in isolation tests and had similar percentage with the reference culture of 41.8% using the API Staph Kit. Heat resistance of Staphylococcus aureus (represented as D value) isolated from green grass jelly at constant heating temperature of D45, D49, D53 and D57 were 32.3, 17.9, 4.6, and 1.5 minutes. On the other hand, D value of isolates ATCC 25923 (standard clinical isolate) at constant heating temperature of D45, D49, D53 and D57 were 18.5, 6.8, 2.9, and 1.4 minutes. The z value of isolates A and ATCC 25923 were 8.8°C and 10.8°C. Smaller z value of isolate A showed that pasteurization process can be accelerated and optimized with increasing the temperature slightly but has the same lethality effect.
PENGARUH MINUMAN BEROKSIGEN TERHADAP SISTEM IMUN, KADAR MALONALDEHIDA DAN PERFORMA RESPONDEN MAHASISWA OLAHRAGAWAN [EFFECT OF OXYGENATED WATER ON IMMUNE SYSTEM, MALONALDEHYDE CONTENT AND STUDENT-ATHLETE PERFORMANCE] Refdi, Cesar Welya; Zakaria, Fransiska Rungkat; Giriwono, Puspo Edi
Jurnal Teknologi dan Industri Pangan Vol. 25 No. 1 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (489.684 KB) | DOI: 10.6066/jtip.2014.25.1.90

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PENGARUH MINUMAN BEROKSIGEN TERHADAP  SISTEM IMUN, KADAR MALONALDEHIDA DAN PERFORMA RESPONDEN MAHASISWA OLAHRAGAWAN[Effect of Oxygenated Water on Immune System, Malonaldehyde Content and Student-Athlete Performance]Cesar Welya Refdi, Fransiska Rungkat Zakaria* dan Puspo Edi GiriwonoDepartemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Bogor Diterima 23 Agustus 2013 / Disetujui 01 Juli 2014ABSTRACT   Exercise requires more oxygen input and cannot be fully obtained through breathing. The body has a homeostatic system, when more ATP is needed, the more oxygen is required by the body. Limited supply of oxygen such as those happens in athlete?s body automatically switches the respiration from aerobic into anaerobic. This situation results in building up of lactic acid which in turn lowers the energy for cellular metabolism as well asenergy for exercise. The purpose of this study was to assess the effects of short-term and long-term drinking of oxygenated water on lactic acid and energy expenditure (EE) in exercise performance; Cluster of Differentiation-4 (CD4), CD8, CD56, Interleukin-6 (IL-6) contents; and level of malonaldehyde. During short-term intervention, 12 male student volunteers drank oxygenated (50, 80 and 130 ppm) water and normal mineral water, 15 minutes before treadmill and after treadmill (10 Kmh). The lactic acid concentration was measured before and after treadmill, and 5 minutes after the reconsumption of the oxygenated water. During long-term intervention, 17 male student volunteers drank oxygenated (100 ppm) water twice a day for 21 days. The blood plasma and lymphocyte sampled before and after intervention were analyzed for the same parameters. The results showed that oxygenated water had no effect on lactic acid accumulations and EE. Similarlyt he long-term study showed that oxygenated water did not affect CD4 and CD8, had no harmful effect on IL-6 and malonaldehyde, yet it significantly increased the CD56 content.  
AKTIVITAS ANTIOKSIDAN DAN PENGHAMBATAN α-GLUKOSIDASE OLEH EKSTRAK ETANOL BAKTERI ASAM LAKTAT INDIGENUS Farida, Eko; Jenie, Betty Sri Laksmi; Nuraida, Lilis; Giriwono, Puspo Edi
Jurnal Teknologi dan Industri Pangan Vol. 30 No. 1 (2019): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (461.223 KB) | DOI: 10.6066/jtip.2019.30.1.56

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Lactic acid bacteria (LAB) are one of Indonesia's biodiversity which can be beneficial for food and health purposes. Some of LAB are potential probiotics with specific functional properties, such as antidiabetes. This study evaluated the effect of ethanol extracts of twelve indigenous LAB in inhibiting ?-glucosidase enzyme and their antioxidant activities. Assay for the ?-glucosidase inhibition was performed on LAB ethanol extract using spectrophotometric method at ?=410 nm, while the antioxidant activity was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method at ?=517 nm. The results showed that ?-glucosidase inhibition was significantly different between the isolates (P<0.01). Lactobacillus fermentum S21209 had the highest ?-glucosidase inhibition activity, which was significantly different from Lactobacillus plantarum MB427, Lactobacillus plantarum Pi28a, Lactobacillus delbrueckii W24802 and Lactobacillus plantarum 2 W22409. Evaluation of the antioxidant activity also showed significant difference between the isolates (P<0.01). Lactobacillus plantarum BSL had the highest antioxidant activity (92.81±1.36%), which was not significantly different from vitamin C as a control. This preliminary study reported that twelve indigenous LAB could be used as potential antidiabetic probiotics, although the responsible compounds are not known.
SENYAWA BIOAKTIF TORBANGUN (Plectranthus amboinicus (Lour.) SPRENG) FRAKSI KLOROFORM INDUKSI APOPTOSIS PADA KANKER PAYUDARA (SEL MCF-7) IN VITRO Yulianto, Wahid; Andarwulan, Nuri; Giriwono, Puspo Edi; Pamungkas, Joko
Majalah Obat Tradisional Vol 22, No 1 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (355.642 KB) | DOI: 10.22146/tradmedj.24312

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Tanaman Torbangun (Plectranthus amboinicus (Lour.) Spreng) telah dimanfaatkan secara tradisional pada berbagai negara di daerah tropis untuk menyembuhkan berbagai penyakit. Penelitian ini bertujuan untuk mengidentifikasi komponen aktif pada fraksi kloroform dari ekstrak tanaman torbangun tersebut yang memiliki efek terhadap ekspresi gen-gen yang berkaitan dengan apoptosis pada sel kanker payudara MCF-7. Apoptosis diamati secara morfologis menggunakan metode pewarnaan inti sel Hoechst. Ekspresi gen dianalisa menggunakan Real-Time PCR sedangkan komponen bioaktif diidentifikasi menggunakan LC-MS. Hasil pengamatan morfologi secara jelas memperlihatkan terjadinya apoptosis pada sel setelah diberi perlakuan ekstrak tanaman. Terjadi peningkatan ekspresi gen anti-apoptosis Bcl-2 namun peningkatan tersebut tidak mampu mencegah terjadinya apoptosis pada sel. Ekspresi gen p53 dan p21 meningkat secara signifikan setelah diberi perlakuan fraksi kloroform. Ekspresi gen caspase 9, caspase 7 dan caspase 1 meningkat dengan level peningkatan yang dipengaruhi oleh konsentrasi yang diberikan. Sebagian besar komponen yang teridentifikasi tergolong dalam kelompok diterpenoid yang kemungkinan berkontribusi terhadap bioaktifitas fraksi kloroform dalam menginduksi apoptosis pada sel kanker MCF-7.
Enterococcus faecium 1.15 Isolated from Bakasam Showed Milk Clotting Activity Putranto, Wendry Setiyadi; Suradi, Kusmajadi; Chairunnisa, Hartati; Mustopa, Apon Zaenal; Giriwono, Puspo Edi; Kusumaningrum, Harsi Dewantari; Suhartono, Maggy Thenawidjaja
ANNALES BOGORIENSES Vol 21, No 1 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (499.913 KB) | DOI: 10.14203/ab.v21i1.293

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The Lactic Acid Bacteria with Milk Clotting Activity (MCA) were isolated from Bakasam, an Indonesian traditional fermented meat. The isolate screening was carried out using modified method of Skim Milk Agar and Milk Clotting Activity Test, and the isolate was then identified using 16S rRNA. We found 4 isolates that showed MCA of 18-20 SU/ml. Identification using 16S rRNA indicated that the isolate ALG.1.15 was 99% (FR3-F primer) and 99% (FR3-R primer) identic with Enterococcus faecium. The isolate potentially produced renin-like protease to subtitute renin from veal.  
The Development of Protein Extract from Local Indonesian Shrimp (Penaeus merquiensis) for Food Allergies Reagent Using Skin Prick Test Method CHALID, SRI YADIAL; SYAH, DAHRUL; GIRIWONO, PUSPO EDI; ZAKARIA, FRANSISKA RUNGKAT
JURNAL ILMU KEFARMASIAN INDONESIA Vol 13 No 2 (2015): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (972.261 KB)

Abstract

Jerbung is a kind of shrimp which widely spread in Indonesian marine that is a major allergen from seafood origin. Until now, the best treatment for food allergies is avoid from all kinds of food allergens. Avoiding certain foods should be established based on allergy tests such as skin prick test (SPT) and double-blind placebo-controlled food challenges. SPT is the most widely used test for detecting IgE-mediated food allergies. The main ingredient of SPT are protein extract which is called the reagent. Jerbung protein was extracted from part of meat by buffer phosphat saline (PBS) then analyzed by using SDS-PAGE and protein concentration was analyzed by Bradford assay. Protein extract was formulated to SPT reagent based on standarization of the European Pharmacopoeia Monograph on Allergen Products 7 (2010:1063). Skin prick test reagent was applied on 24 adult participants who were food allergies and 16 adults individuals of non food allergies. Sera of subjects were collected to measuretotal IgE and specific IgE-binding. The sensitivity of protein extract was 95% and negative error of 5%. Whereas specificity was 94% with a positive error was 6%. Immunobloting was also performed on 10 sera subjects. Nine sera subjects of these shrimp allergic participants showed the specific binding to allergen of jerbung shrimp with the range of molecular weight of 28-63 kDa.
Role of Nutrition and Health Claims on Growing Up Milk Product as Purchasing Decision Determination RTR, Ayu Puspitalena; Palupi, Nurheni Sri; Giriwono, Puspo Edi
Jurnal Mutu Pangan (Indonesian Journal of Food Quality) Vol 2, No 2 (2015)
Publisher : Jurnal Mutu Pangan (Indonesian Journal of Food Quality)

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Abstract

Abstract. . This moment,it has not been known the role of nutrition and health claims as purchasing decision determination. Objective of the research is to study the role of nutrition and health claims on the labels of growing up milk as purchasing decision and to analyze corellation of respondent's characteristics which have influence. Study was conducted by survey, data were analyzed descriptively and statistically. Label of growing up milk for children 1-3 years old read by 70% respondent and 30% occasionally, and 23% of respondents do not read nutrition and health claims. Nutritional and health claims have a role as a determinant of purchasing decisions for consumers of 24%. As for the 76% purchase decision is influenced by other factors such as suitable with a child, nutrient content, good taste or price. The role of nutritional and health claims as a determinant of purchase decisions are influenced by factors of education, family expenses per month and expenditure for milk per month. As for the other factors that include age, occupation, number of children, place of purchase, and frequency of purchases did not affect the role of nutrition and health claims as a determinant of purchase. Keywords: Label, growing up milk, nutrition and health claims, purchasing decisions Abstrak. Saat ini belum diketahui peranan klaim gizi dan kesehatan sebagai penentu keputusan pembelian. Penelitian bertujuan mengkaji peranan klaim gizi dan kesehatan pada label produk susu pertumbuhan terhadap keputusan pembelian dan menganalisis hubungan karakteristik responden yang berpengaruh. Penelitian dilakukan dengan metode survei, data dianalisis secara deskriptif dan statistik. Sebanyak 70% responden membaca label produk susu pertumbuhan untuk anak usia 1-3 tahun dan 30% kadang-kadang, serta 23% responden tidak membaca klaim gizi dan kesehatan. Klaim gizi dan kesehatan mempunyai peranan sebagai penentu keputusan pembelian bagi konsumen yaitu sebesar 24%. Sebesar 76% keputusan pembelian dipengaruhi oleh faktor lainnya seperti cocok dengan anak, kandungan gizi, rasa yang enak ataupun harganya. Peranan klaim gizi dan kesehatan sebagai penentu keputusan pembelian dipengaruhi oleh faktor pendidikan, pengeluaran keluarga per bulan dan pengeluaran untuk susu per bulan. Faktor lain yang meliputi usia, pekerjaan, jumlah anak, lokasi pembelian, dan frekuensi pembelian tidak mempengaruhi terhadap peran klaim gizi dan kesehatan sebagai penentu pembelian. Kata kunci: Label, susu pertumbuhan, klaim gizi dan kesehatan, keputusan pembelian Aplikasi Praktis: Klaim gizi dan kesehatan pada label susu pertumbuhan untuk anak usia 1-3 tahun berperan sebagai penentu keputusan pembelian. Namun demikian belum semua responden membaca label serta klaim gizi dan kesehatan karena kurang dapat dipahami. Hasil ini dapat digunakan sebagai bahan pertimbangan bagi badan regulasi untuk mengatur klaim gizi dan kesehatan dalam bahasa yang lebih dapat dimengerti oleh masyarakat. Bagi produsen bermanfaat untuk dapat menentukan klaim gizi dan kesehatan yang tepat pada label sehingga berperan efektif sebagai pertimbangan dalam memilih produk. http://jurnalmutupangan.com/index.php?link=view&id=JMP-02-17-037
Prevalensi Penyakit Kanker di Indonesia Berdasarkan Pola Konsumsi Pangan dan Gaya Hidup (Cancer prevalence in Indonesia Based on Food Consumption Patterns and Lifestyle) Aliwikarta, Kuswanto; Palupi, Nurheni Sri; Giriwono, Puspo Edi
Jurnal Mutu Pangan (Indonesian Journal of Food Quality) Vol 3, No 1 (2016)
Publisher : Jurnal Mutu Pangan (Indonesian Journal of Food Quality)

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Abstract

Consumption of processed meat is associated to cancer risk increasing. This study used secondary data from RISKESDAS 2013 with a case-control study design, is aimed to analyze the cancer prevelance in Indonesia based on food consumption patterns and lifestyles. The case population is 90 people suffering from cancer and 90 people with no cancer randomly selected as control. Respondent profiles were analyzed by univariate analysis. The relationship of cancer risk factors were analyzed by bivariate analysis and determination of risk factors using multivariate analysis. Sensitivity and specificity analyses showed the cancer vulnerable age is ≥46 years. Processed meat consumption is associated significantly (p=0.000; OR=1.25) in the cancer prevelance. Respondents frequently eat processed meat risk is predicted 1.25 times compared to those not taking. Vegetable consumption (p=0.046; OR=0.509) and fruit consumption (p=0.022; OR=0.365) related significantly to cancer. Vegetables consumption reduce the risk by 0509 times, and fruit consumption reduce the risk by 0365 times. Lifestyle risk factors (physical activity and smoking) significant (p=0.000) to cancer. Multivariate analysis results showed the age, gen- der and processed meat consumption is associated significantly (p=0.000) to cancer. 
Pengembangan Mi Kering Berbahan Dasar Tepung Ubi Jalar Ungu (Ipomoea batatas L.) sebagai Pangan Fungsional Tinggi Serat Lidwina, Lidwina; Giriwono, Puspo Edi; Rimbawan, Rimbawan
Jurnal Mutu Pangan (Indonesian Journal of Food Quality) Vol 5, No 1 (2018)
Publisher : Jurnal Mutu Pangan (Indonesian Journal of Food Quality)

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Indonesia is currently experiencing double nutrition problems, both malnutrition and over nutrition. Over nutrition can lead to obesity and degenerative diseases. One solution to overcome this problem is developing high-fiber food products which helps to improve gastrointentestinal function. Sweet potato flour is used as a source of fiber and a source of carbohydrate. Several formulas were designed and those with the highest content of sweet potatoes and acceptance score were further analyzed. Formula 1 has a water content of 14.08%, ash content 7.11%, fat content 0.36%, protein content 7.56%, and carbohydrate content of 71.11%. Cooking loss in F1 was 19.34%. Results of the analysis showed that total phenolic content of F1 has amounted to 27.0 mg GAE/100 g. While the result of total dietary fiber content analysis was 14.65% dw or equal to 12.58% ww. However, the highest acceptance score is in Formula 3. This formula contains moisture content of 13.51%, ash content 7.07%, fat content 0.13%, protein content 9.02%, and carbohydrate content of 70.33%. Cooking loss of this formula was 13.84%. Results of the analysis showed that total phenolic content of F3 was 15.9 mg GAE/100 g. Analysis of dietary fiber content showed 13.32% dw or equal to 11.52% ww. Based on Indonesian regulations, the fiber content of both formulas can be regarded as high fiber dry noodles as in contains no less than 6 g per 100 g.
Gerakan Scaling-up Nutrition (SUN): Meningkatkan Kerjasama Kemitraan Multi Stakholder dalam Mengatasi Tantangan Kekurangan Zat Gizi di Indonesia (Scaling-up Nutrition (SUN) Movement: Enhancing Multistakeholder Partnership to Address Nutrition Challenge in Indonesia) Giriwono, Puspo Edi; Indrayana, Stefanus
Jurnal Mutu Pangan (Indonesian Journal of Food Quality) Vol 2, No 1 (2015)
Publisher : Jurnal Mutu Pangan (Indonesian Journal of Food Quality)

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Average protein intake of Indonesian is relatively low. Black bean (Phaeseolus vulgaris) is one of the Indonesia local pulses containing high content of protein (16.6% wet basis). Black bean our has a potential as a substitute for wheat our in bakery products such as brownies. The objective of this research was to determine suitable milling procedure of black bean and to evaluate black bean our substitution on physical, chemical, and sensory effects of of brownies. Two different milling methods (dry and wet milling) were studied to produce black bean our after different soaking times (6, 12, and 18 hours). Dry milling method was optimum after 12 hours of soaking. Black bean our contained 23.9% of protein, 62.61% of carbohydrate. The yield of milling process was 49.39% and the our whiteness was 57.74. Substitution 25% of black bean our in brownies making did not decrease brownies characteristics. The brownies had 7.8% of protein content, 513.13 gram force of hardness, 0.71 of elasticity, 0.36 of cohesiveness, with acceptable sensory qualities. Addressing malnutrition in the global scale requires intiatives beginning from every individual country level. This approach in alleviating national nutritional issues at the domestic population contributes signi cantly to the overall worldwide mission to eradicate malnutrition and its detrimental effects which has been outlined in the Millenium Development Goals (MDG) established at the turn of the century. Overcoming issues in nutrient de ciencies becomes the responsibility of all stakeholders, government, industry, academic and other organizations, and include most important of all, the general public. In this integrated approach, an initiative known as the Scaling-Up Nutrition movement is gaining wide recognition and momentum in addressing nutritional problems the world faces. One course of action that may be “scaled-up” involving all parties, is forti cation of food products as an ef cient and effective effort to alleviate nutritional de ciencies, more often than not, de ciencies in micronutrients. Some challenges are expected in this avenue, such as sustainable support for forti cation programs in the form of multi-stakeholder partnerships, effecting policy change to support mandatory forti cation, long- term monitoring of the programs’ compliance and ef cacy in light of limited resources, and increasing awareness and uptake of forti ed products through social marketing campaigns. Thus what is required is the development of terms of governance and agreement for multi-stakeholder partnerships, supporting sustainable business model and its monitoring, for effectiveness and for enforcement of fortification legislation.