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Acid Resistance Test of Probiotic Isolated from Silage Forage Swamp on In Vitro Digestive Tract Sandi, Sofia; Miksusanti, Miksusanti; Sari, Meisji Liana; Sahara, Eli; Supriyadi, Adi; Gofar, Nuni; Asmak, Asmak
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 4, No 1 (2019): February 2019
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24845/ijfac.v4.i1.15

Abstract

Antibiotics are commonly used in poultry ration, and afterward these drug residues may persist in foods. There is an alternative source of antibiotics in poultry rations, one of which is using probiotics. Probiotics are living microorganisms capable of reaching the gastrointestinal tract and benefiting health, leaving no residue in the body. Probiotics can be originated from bacteria such as lactic acid bacteria (LAB) which produced lactic acid and antimicrobial components. LAB must be able to live in the digestive system with various pH condition. pH resistance testing of LAB was performed by introducing diluted bacteria into several buffer solutions with different pH and a predetermined incubation time, then was grown in MRS agar medium. This study was conducted to determine the resistance of LAB isolates as probiotics from silage forage swamp at different pH distributed in vitro digestant. The design used was a complete randomized design consist of 3 treatments and 3 replications. The treatments were using isolate of Kumpai Tembaga silage (P1), isolate of 50% Kumpai Tembaga silage and 50% Kemon Air silage (P2), and isolate of Kemon Air silage (P3). The results showed that all isolates used were lactic acid bacteria, and the treatment significantly affected the value of LAB resistance test against low pH and high pH. Isolate from Kumpai Tembaga silage (P1) is a lactic acid bacterium with higher ability to survive in pH of in vitro digestive system.
Methane Gas Production Test From Forage Swamp with Ensilase Method As Biogas Plants Kartika, Erra; Gofar, Nuni; Sandi, Sofia
Sriwijaya Journal of Environment Vol 2, No 3 (2017): Low Land
Publisher : Program Pascasarjana Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22135/sje.2017.2.3.72-75

Abstract

This study aims to test the production of methane gas from forage swamps by ensilase as biogas plants. Treatment levels consisted of 3 treatments P1 (100% Kumpai tembaga grass (Hymenachne acutigluma)), P2 (50% Kumpai tembaga grass (Hymenachne acutigluma) + 50% Kemon air (Neptunia oleracea lour)), P3 (100% Kemon air (Neptunia Oleracea lour)) and 5 replications. The result of the diversity analysis showed that silage swamp silage ensilase process significantly (p <0,05) to methane gas formation. The best composition was obtained from the treatment of P1 Kumpai tembaga grass (Hymenachne acutigluma) 100%.Keywords: Production, Methane, Forage Swamps, Ensilase, Biogas Plants.
EKSPLORASI BAKTERI ANTAGONIS ASAL JARINGAN DAN RIZOSFER TANAMAN KARET UNTUK MENEKAN PERTUMBUHAN BAKTERI PROTEOLITIK PADA BAHAN OLAHAN KARET (BOKAR) Gofar, Nuni; Munawar, Munawar; Widjajanti, Hary; Mulya, Angga Prasetya
Jurnal Ilmu Tanah dan Lingkungan Vol 16 No 2 (2014): Jurnal Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (432.176 KB) | DOI: 10.29244/jitl.16.2.61-66

Abstract

Lateks merupakan komoditas pertanian andalan dan merupakan sumber penerimaan devisa negara Indonesia yang cukup penting, namun pada saat penyimpanannya menyumbangkan bau tidak sedap ke udara. Penelitian ini bertujuan untuk mengeksplorasi bakteri antagonis asal jaringan dan rizosfer tanaman karet yang mampu menekan pertumbuhan mikroba proteolitik penyebab bau tidak sedap pada bahan olahan karet (bokar). Bakteri proteolitik penyebab bau tidak sedap diisolasi dari berbagai masa simpan bokar. Bakteri antagonis diisolasi dari jaringan dan rizosfer tanaman karet. Kemampuan bakteri antagonis menekan pertumbuhan bakteri proteolitik dilakukan dengan uji daya hambat dalam medium agar (NA). Diameter zona bening yang terbentuk merupakan indikasi kemampuan bakteri antagonis dalam menghambat aktivitas bakteri proteolitik. Dari hasil penelitian ini diperoleh 2 isolat bakteri yang berpotensi dikembangkan sebagai bakteri antagonis penghambat aktivitas bakteri proteolitik perombak bokar dalam proses penyimpanan. Kedua bakteri antagonis tersebut adalah isolat D1U1 yang berasal dari daun tanaman karet dan isolat A2U2 yang berasal dari akar tanaman karet, yang menghasilkan rata-rata zona hambat terluas berturut-turut berdiameter 11.37 dan 11.29 mm.
PENGARUH KOMBINASI PUPUK ORGANIK DAN HAYATI TERHADAP PERTUMBUHAN DAN PRODUKSI GALUR JAGUNG (ZEA MAYS. L) HASIL SELEKSI EFISIEN HARA PADA LAHAN KERING MARGINAL Moelyohadi, Yopie; Harun, M. Umar; Munandar, Munandar; Hayati, Renih; Gofar, Nuni
Jurnal Lahan Suboptimal: Journal of Suboptimal Lands Vol 2, No 2 (2013)
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (453.735 KB) | DOI: 10.33230/JLSO.2.2.2013.50

Abstract

This study aimed to study the growth and yield of maize crop nutrient  efficient  selection against  the  combination of different types of organic fertilizers and bio-fertilizers at low dose levels of chemical fertilizer on marginal dry land for the development of technological innovation fertilization on maize crops to reduce the use of chemical fertilizer on marginal dry land. This study was conducted in the field trials of Agro Techno Park (ATP), the Ministry of Research and Technology, South Sumatra from  January  to May  2012.   The experimental design used was SplitPlotdesign with three replications.   . The main plot treatment was dose levels of chemical fertilizer consists of : P1 = 50 % standard dose of ATP ( 200 kg urea , 50 kg SP36 and 25 kg KCl/ha ) and P2 = 25 % standard dose of ATP ( 100 kg urea , 25 kg SP36 KCl and 1.25 kg/ha) . treatment subplot , is the combined delivery of this type of organic fertilizer + biofertilizer types , comprising : KHO = control ( without organic fertilizer + biofertilizer ) , KH1 = composted cow manure + mycorrhiza , KH2 = composted cow manure + bacterial phosphate solvent , KH3 = composted chicken manure + biological mycorrhizae , KH4 = composted chicken manure + bacterial phosphate solvent , KH5 = compost straw corn + mycorrhizae , KH6= composted maize straw + bacteria phosphate solvent , KH7 = compost Legume cover crop ( LCC ) + Mycorrhiza and compost KH 8= legume cover crop (LCC) + solvent bacteria Phosphate.  The results showed that chicken manure compost fertilizer + fertilizer mycorrhizal give the best effect on the growth and yield of dryland corn yields marginal with an average of 9.70 tons of dry cobs/ha and the combined treatment of chemical fertilizer at 50 % level and composting chicken manure + mycorrhizal give the best effect on the growth and yield of maize in marginal dry land , with an average yield reached 10.51 tons of dry seed/acre.
PEMANFAATAN BERBAGAI JENIS PUPUK HAYATI PADA BUDIDAYA TANAMAN JAGUNG(ZEA MAYS. L) EFISIEN HARA DI LAHAN KERING MARGINAL Moelyohadi, Yopie; Harun, M. Umar; Hayati, Renih; Gofar, Nuni
Jurnal Lahan Suboptimal: Journal of Suboptimal Lands Vol 1, No 1 (2012)
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (402.47 KB) | DOI: 10.33230/JLSO.1.1.2012.6

Abstract

Moelyohadi et al., 2012. The Use of  Various Types of Biofertilizers on Cultivation of  Nutrient Efficient Corn Genotypes(Zea mays L.  in Marginal Dry Land. JLSO 1(1):31-39.The study aimed at  obtaining nutrient efficient corn genotypes that give the best response to various types of biological fertilizers at low-level doses of chemical fertilizer in marginal drylands. This study was conducted in the field trials of Agro Techno Park (ATP), the Ministry of Research and Technology, South Sumatra from May to September 2011. The experimental design used was SplitPlotdesign  with three replications. The main plot treatments consisted of: (H0): without biofertilizer, (H1): biofertilizer:-mycorrhizae and (H2): BPF biological fertilizers. Subplot treatments, consisting of three corn genotypes for the selection of efficient nutrient properties, are genotypes  B-41 (G1), L-164 (G2), S-194 (G3)  and onehybrid variety:BISI-816 (G4) as agenotypecomparator. Alltreatmentunitswere givenlow dosesof chemicalfertilizerthat is 50% of theATPstandarddose(200kgUrea, SP-36 50kgand 25kgKClha-1).  The results showed that mycorrhizal fertilizer produced the highest corn production, which is 6.08 ton dry seed / acre and genotype B-41 shows a more adaptive growth in marginal dry land with a production  of 7.27 tons of dry  seed /acre  and the combined treatment of mycorrhizal fertilizer and genotype B-41 gave the highest production  of 8.57 tons of dry seed / acre 
PERTUMBUHAN DAN HASIL PADI PADA INSEPTISOL ASAL RAWA LEBAK YANG DIINOKULASI BERBAGAI KONSORSIUM BAKTERI PENYUMBANG UNSUR HARA Wuriesyliane, Wuriesyliane; Gofar, Nuni; Madjid, Abdul; Widjajanti, Hary; Putu SR, Ni Luh
Jurnal Lahan Suboptimal: Journal of Suboptimal Lands Vol 2, No 1 (2013)
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (570.646 KB) | DOI: 10.33230/JLSO.2.1.2013.32

Abstract

The aim of this research was to study the effect of multisynergism bacterial isolates growth of rice plants as well as to obtain best composition of endophytic bacterial isolates, Azotobacter, Azospirilium and phosphate solubilizing bacteria in spurring growth of rice plants. This research was conducted in October 2012 to February 2013. This study arranged in a completely randomized design (All treatments of multiple bacterial isolates resulted in increase of soil N x P Content). The results showed that multibacterial isolates B (endophytic bacteria + Azospirillum) increased vegetative and generative growth of rice plants. Treatment G (composition of endophytic bacterial isolates, Azotobacter, Azospirilium and phosphate solubilizing bacteria) can increase rice yields. Some soil N and P content increased in response to all treatments of multiple bacterial isolates. The similar result was also observed in the N and P uptake that some multiple bacterial isolates improved N and P uptake of plants
APLIKASI ISOLAT BAKTERI HIDROKARBONOKLASTIK ASAL RIZOSFER MANGROVE PADA TANAH TERCEMAR MINYAK BUMI Gofar, Nuni
Jurnal Lahan Suboptimal: Journal of Suboptimal Lands Vol 1, No 2 (2012)
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (535.076 KB) | DOI: 10.33230/JLSO.1.2.2012.17

Abstract

Gofar. 2012. Application of Hydrocarbonoclastic Bacteria Isolates from Mangrove Rhizosphere on Petroleum Polluted Soil. JLSO 1(2):123-129.This research was aimed at studying the ability of hydrocarbonoclastic bacteria to reduce total petroleum hydrocarbon of petroleum polluted soil. The hydrocarbonoclastic bacteria were isolated from rhizosphere of mangrove grown on petroleum contaminated soil in Sungsang, Sumatra Selatan. The samples were taken using a defined sampling method from the oil contaminated areas. Soil samples around the roots of mangrove plants were randomly taken in the contaminated    area.  Isolates obtained from the isolation and selection of bacteria from mangrove forests were overhauled at the laboratory-scale using oil-enriched medium following  a completely randomized design. Nine isolates of hydrocarbonoclastic bacteria were found to be  capable of growing on petroleum contained medium in vitro. Two best isolates in degrading petroleum hydrocarbon compound were Pseudomonas alcaligenes (I5) and Alcaligenes facealis (I8). P. alcaligenes and A. facealis were able to decrease TPH up to 63% and 70% respectively. The ability of these isolates in degrading hydrocarbon compound was 6.5-7.0 times higher than the control. 
KERAGAMAN BEBERAPA SIFAT KIMIA DAN BIOLOGI TANAH PADA BERBAGAI TIPE PENGGUNAAN LAHAN RAWA LEBAK GOFAR, NUNI
AGRITROP Vol. 26, No. 2 Juni 2007
Publisher : AGRITROP

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The purposes of this study were to study: 1) the influence of agricultural land use on some swamp soil chemical (soil pH, Organic C content, N-total, and P-total) and biological (population of bacteria, fungi, and actinomycetes) properties, and 2) correlation between soil microbial population and Organic C content, N-total as well as P-total. Based on the results of field observation, it was determined 6 plant commodities with different length of land use as treatments and secondary forest as control. The results of this study showed that: 1) Differences in land use resulted in different soil chemical (soil pH, Organic C content, N-total, and P-total) and biological (population of bacteria, fungi, and actinomycetes) properties, 2) Soil organic C, N-total and P-total content together significantly affected on population of bacteria and fungi, but did not affected on actinomycetes population. The magnitudes of Soil organic C, N-total and P-total influence on bacteria, fungi, and actinomycetes population were 69,8 %, 59,8 % and 49,7 %, respectively, 3) as soil organic C and P-total contents were constant, soil N-total content significantly affected on population of bacteria and fungi, but did not affected on actinomycetes population, 4) Population of bacteria, fungi and actinomycetes was highly correlated with soil organic C and N-total content, but was not correlated with soil P-total content, 5) the most microbial variation was found on soil collected from rubber plantation, followed by orange and secondary forest.
Stimulate The Growth of Rice Using Endophytic Bacteria from Lowland Rice Plant Tissue Gofar, Nuni; Widjajanti, Hary; Marlina, Neni
Sains Tanah - Jurnal Ilmu Tanah dan Agroklimatologi Vol 12, No 2 (2015)
Publisher : Faculty of Agriculture, Sebelas Maret University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15608/stjssa.v12i2.250

Abstract

Exploration and selection of endophytic bacteria from healthy food crops grown in lowland ecosystem is important to be conducted in order to get growth-stimulating endophytic bacteria at soil with low fertility level so that capable to optimize initial growth of food crops and subsequently can increase productivity level of lowland soil.The research objective was to isolate and to test the IAA-producing endophytic bacteria isolate in stimulating the rice crop growth at lowland area. Endophytic bacteria are isolated from tissues of rice, corn and peanut crops which grown at shallow swamp land in Ogan Ilir and Ogan Komering Ilir Districts, South Sumatra, Indonesia. There was nine isolates of nitrogen-fixer endophytic bacteria that capable to contribute IAA phytohormone into their growth media. The P31 isolate from rice crop tisssue of 2 months old produce the best rice sprouts than other isolates. This isolate can contribute of about 10 mg kg-1 IAA to its growth medium  and increase the crowns dry weight and the roots dry weight respectively with magnitudes of 133% and 225% compared to control treatment. Concentration and absorbtion of N for rice crops innoculated with P31 isolates had increased by 169% and 400%, recpectively. The P31 isolates had been identified as Burkholderia pseudomallei (also known as Pseudomonas pseudomallei).
The Population of Bacteria and CO2 Release on Process of Composting Manure and Swamp Grass Utama, Diana; Gofar, Nuni; Siti Nurul Aidil, Fitri
Sriwijaya Journal of Environment Vol 2, No 1 (2017): Green Environment For Human Welfare
Publisher : Program Pascasarjana Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aimed to analyze the bacteria population, the release of CO2, pH and organic-C and total-N in the process of compost made from manure and swamp grass. Treatment level consist of 100% manure (K100), 50% of manure + 50% swamp grass (K50R50), 25% of manure + 75% swamp grass (K25R75), and 10% of manure + 90% swamp grass (K10R90). The result of this study indicated the dynamic of different bacteria population on different composting materials by increasing of the composting time. The release of CO2 decrease on all treatment levels  by increasing of the composting time. The pH value increased at all levels of treatment, except the composition of 100% manure. The best composition obtained by mixing of 10% manure and 90% swamp grass.Keywords: CO2 release, compost, manure, population, swamp grass.