Found 2 Documents
Journal : Science and Technology Indonesia

Bioremediation of Spent Bleaching Earth (SBE) Wastes using Lipolitic Bacteria (Bacillus cereus) with Variation of Inoculum Volume Lusia, Maria; Gofar, Nuni; Widjajanti, Hary
Science and Technology Indonesia Vol 3 No 1 (2018): January
Publisher : ARTS Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.782 KB) | DOI: 10.26554/sti.2018.3.1.35-40


Spent bleaching earth (SBE) is a solid waste was generated from the CPO refining step into cooking oil.  SBE that was discharged directly into the environment has the potential to pollute the environment, because in the SBE waste contained oil and acid residues, which are easily to be oxidized and flammable.  Therefore, SBE must be processed first before being discharged into the environment.  One way to manage SBE is by bioremediation.  Bioremediation is a method on cleaning the environment from contaminants by using  biological agents, such as bacteria, fungi etc.  The bacterial isolates used in this study were Bacillus cereus.  This study aims to obtain the best inoculum and to know the ability of Bacillus cereus bacteria in degrading the oil content in SBE waste. This study used Completely Randomized Design with the volume of Bacillus cereus bacteria inoculum as a treatment, consisting of 6 treatment levels of 0 mL kg-1, 25 mL kg-1, 50 mL kg-1, 75 mL kg-1, 100 mL kg-1, 125 mL kg-1.  Each treatment level was repeated 3 times, so taht 18 experimental units were obtained.  Observation was done once a week, in a month.  Parameters observed were bacterial population, percentage of oil degradation, and oil content degradation.  The best treatment result for the bacterial population was obtained at the treatment of 100 mL kg-1, at week 4 which was 7,4 x 108 cfu g-1, and for the oil degradation was obtained at 50 mL kg-1 on the treatment at week 4 as big as 90,43%.
Potential of Anabaena Azollae Extract as Antimicrobial Agent For Paddy Crop Disease Gofar, Nuni; Diana, Andi; Setianingsih, Eka
Science and Technology Indonesia Vol 3 No 4 (2018): October
Publisher : ARTS Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (745.042 KB) | DOI: 10.26554/sti.2018.3.4.183-188


The research objective was to test antimicrobial activity from compound produced by A. azollae which symbiosis with Azolla sp. toward microbial test of X. oryzae and Pyricularia oryzae as cause of disease on paddy crop. Sampling of Azolla which symbiosis with A. azollae was done in Azolla cultivation pond at Faculty of Agriculture, Sriwijaya University. The available Azolla was consisted of two types, i.e. Azolla pinnata and Azolla mycrophiylla. The extraction of A. azollae from Azolla leaves was done by method of Ultrasound Assisted Extraction (UAE) using ethyl ester solvent and maseration method using methanol solvent. Test media for bacteria and fungus respectively are Mueller Hilton Agar (MHA) and Sabouraud Dextrose Agar (SDA). The diameter of produced clear zone is an indication of extract’s inhibitory power toward bacteria or fungus. The different of inhibitory zone diameter is compared by using t-test. Analysis of active compounds on Anabaena azollae extract was done by using HPLC. Ethyl acetate or methanol extract of A. azollae which symbiosis with A. pinnata or A. microphylla was capable to inhibit the growth of X. oryzae bacterium and P. oryzae fungus. The dominant compounds containing within extract with probability more than 60% and area of more than 10% are consisted of phytol, hexadecanoate acid and 2-(tert-butyl)-4,6-dimethyl phenol.