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PENAPISAN BAKTERI ENDOFIT AKAR KEDELAI SECARA IN PLANTA UNTUK MENGENDALIKAN PENYAKIT PUSTUL BAKTERI Habazar, Trimurti; Resti, Zurai; Yanti, Yulmira; Trisno, Jumsu; Diana, Afrika
Jurnal Fitopatologi Indonesia Vol 8 No 4 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (714.273 KB) | DOI: 10.14692/jfi.8.4.103

Abstract

Endophytic bacteria reside within plant hosts without causing disease symptoms. Bacterial endophytes promote plant growth and yield, suppress pathogens, may help to remove contaminants, solubilize phosphate, or contribute assimilable nitrogen to plants. The aim of this study were to obtain indigenous endophytic bacterial isolates, which have the ability to control bacterial pustule cause by Xanthomonas axonopodis pv. glycines and to increase growth and yield of soybean. The bacterial endophyte isolates were introduced as seed treatment (108 cfu/ml) and soil drench on 3 weeks old soybean seedling. Xanthomonas axonopodis pv. glycines were inoculated on one month old of soybean by pricking leaf methodes. The effect of bacterial endophyte on disease incidence, disease severity, plant growth and yield of soybean were evaluated. We have found two selected bacterial endophyte isolates from soybean?s rhizosphere (St4E1.1 and St1E1.1.), with the ability to control bacterial pustule and to increase growth and yield of soybean.Key words: bacterial endophyte, bacterial pustule, in planta technique, soybean
PENGARUH SUHU TERHADAP PERKEMBANGAN PRADEWASA PARASITOID ERIBORUS ARGENTEOPILOSUS CAMERON (HYMENOPTERA: ICHNEUMONIDAE) Nelly, Novri; Habazar, Trimurti; Syahni, Rahmat; Buchori, Damayanti
Jurnal Natur Indonesia Vol 13, No 3 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (55.996 KB) | DOI: 10.31258/jnat.13.3.250-255

Abstract

Temperature effect on development time of the preadult parasitoid Eriborus argenteopilosus Cameron(Hymenoptera: Ichneumonidae) were studied to know development time, degree days and survival rate.Crocidolomia pavonana (Fabricius)(Lepidoptera: Pyralidae) larvae was exposed to E. argenteopilosus female andreared at four different temperatures i.e 160C, 200C, 250C and 300C. Data were analyzed using anova and linearregression to calculate degree day. At 200C E. argenteopilosus showed the highest degree day and survival rate(18.67 %), while at 300C nothing adult parasitoid emergenced. Degree day to development time of parasitoid attemperature 200C i.e fase egg-adult: 300.05; egg-pupae 173.35; pupae-adult 171.
INOKULASI FUNGI MIKORIZA ARBUSKULA (FMA) INDIGENUS PADA BIBIT JAHE UNTUK PENGENDALIAN PENYAKIT LAYU RALSTONIA SOLANACEARUM RAS 4) Suharti, Netty; Habazar, Trimurti; Nasir, Nasril; Dachryanus, Dachryanus; Jamsari, Jamsari
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (49.568 KB) | DOI: 10.31258/jnat.14.1.61-67

Abstract

Bacterial wilt disease caused by Ralstonia solanacearum race 4 is one of factors inhibiting ginger production. The study on ArbuscularMychorrizae Fungus (AMF) in greenhouse was found could reduce of bacteria wilt disease. Experiment design of research was randomizedblock design using 8 isolates. The results showed that inoculation of Arbuscular Mychorrhizae Fungus isolated from healthy gingerrhizosphere as biological diseases control agents could reduces disease severity. Four isolate could reduced disease severity up to 100% andsupport plant growth and production. As the result the seedling formation increased by 50?150%, plant height 36.92?87.56%, the numberof leaf 61.94?162.22% and ginger yield 190.62?400% respectively, compared to the control.
PERUBAHAN BENTUK PLANLET PISANG RAJA SEREH HASIL MUTASI DENGAN ETHYL METHANE SULPHONATE (EMS) SECARA IN VITRO Yanti, Yulmira; Habazar, Trimurti; ', Mardinus; ', Mansyurdin
Jurnal Natur Indonesia Vol 11, No 2 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (28.546 KB) | DOI: 10.31258/jnat.11.2.104-108

Abstract

The shoot of ?rajasereh? banana was treated by 0,2% and 0,5% of ethyl methane sulfonate (EMS), for 2 and 4 hoursthourgh in vitro. The results showed that treatment of EMS mutagen would be changed morphologycal characterseither in planlet. In planlet stage was obtained four morphololgycal variations. One of the variant have characterizedthe colour yellowish; the leaves and stem are yellowish: the leaves were small and spiral. The value of coefficientof variant for morfphologycal characters of planlet ?raja sereh? banana increased compare to the control. Theextreme value of coefficient of variant in planlet stage was found the times shoots is 84,31%, while control that is8,24%. EMS mutagen could caused several planlets shorter, total of leaves could of shoot are mostly
SENYAWA PHENOLIK AKAR PISANG CV. KEPOK (MUSA ACUMINATA) YANG DIINDUKSI DENGAN FUNGI MIKORIZA ARBUSKULAR INDIGENUS PU10-GLOMUS SP 1 TERHADAP PENYAKIT DARAH BAKTERI Suswati, Suswati; Habazar, Trimurti; Husin, Eti Farda; Nasir, Nasril; Putra, Dedi Prima; Taylor, Peter
Jurnal Natur Indonesia Vol 13, No 3 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.413 KB) | DOI: 10.31258/jnat.13.3.207-213

Abstract

Cooking banana (Musa acuminata) cv. Kepok is the most susceptible to Blood disease bacterium (BDB) infection.From previous study revealed the best isolate indigenous Arbuscular Mycorrhiza Fungi-Pasar Usang 10 (PU10-Glomus sp 1) could induce cv.Kepok resistance to BDB in green house and field experiment. The AMF could changethe phenolic compound in root plant. This objectives were to measure the root phenolic compound and bioassayto BDB. The 50 grams fresh inoculant PU10-Glomus sp 1 were applicated to banana root plants 60 days old with 6levels time course: 12; 24; 36; 48; 72; 92 hours and control (without PU10-Glomus sp 1). The root methanolicextraction followed to Echeverri et al., (2002) methode with vacuum concentration of the filtrate and partitioninginto ethyl acetate revealed the presence of an antibacterial compound as detected by TLC (Thin LayerChromatography), assay phenolic contained by Spectrofotometer UV-Vis 1700. PharmaSpec. Shimadzu andbioassay using BDB. Nine antibacterial compounds rose from root banana seedling colonized by PU10-Glomus sp1 in 12 hours after applicated (haa) ; 24; 36 and 48 haa. They were with Rf values of 0.16; 0.17; 0.19; 0.26; 0.32; 0.37;0.71; 0.80 and 0.83 on silica plates run in hexane:ethyl acetate (1:2 v/v) and control contained only 0.05 and 0.28.These compounds produced fluorescens which was bright yellow green spots and purple and have antimicbrobialproperties to BDB.
Potensi serangga pengunjung bunga sebagai vektor penyakit darah bakteri (Ralstonia solanacearum Phylotipe IV) pada pisang di Sumatera Barat Mairawita, Mairawita; Habazar, Trimurti; Hasyim, Ahsol; Nasir, Nasril; Suswati, Suswati
Jurnal Entomologi Indonesia Vol 9, No 1 (2012): April
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.9.1.38

Abstract

Blood diseases caused by Ralstonia solancearum Phylotype IV is a major cause of production loss of banana in Indonesia, particularly for areas in West Sumatera. Currently there is a lack of information on blood diseases. The objective of this study was to obtain data of insect diversity and its potential as a dissemination agent of R. solanacearum Phylotype IV in West Sumatera. This research was conducted with a purposive sampling method in the Tabek Panjang highlands, District of Baso, Agam Regency and Pasar Usang lowland, district of Batang Anai, Pariaman Regency, West Sumatera. The results showed that banana plants infected by R. solanacearum Phylotype IV have a high diversity of flower-visiting insects. The diversity of insects in the lowlands is higher than that in highland and mainly are dominated by Trigona spp. (Hymenoptera: Apidae), Drosophila sp. (Diptera: Drosophilidae). To test the potential of both insect as vector of the blood diseases, isolated and identification of the bacteria using triphenyl tetrazolium medium chlorid (TTC) was used. Result showed that bacteria isolated from both insect are R. solanacearum hence providing evidence of the insect as vector of the blood diseases. Both insects have the potential to be vector of R. solanacearum Phylotype IV in West Sumatera. The identification of the bacteria that causes the disease. The identification of bacteria that is spread by flower visitors insects are R.solanacearum Phylotipe IV.
INDUKSI KETAHANAN TANAMAN JAHE TERHADAP PENYAKIT LAYU Ralstonia solanacearum RAS 4 MENGGUNAKAN FUNGI MIKORIZA ARBUSKULAR (FMA) INDIGENUS Suharti, Netty; Habazar, Trimurti; Nasir, Nasril; Dachryanus, .; Jamsari, .
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 11, No 1 (2011): Maret, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (585.073 KB) | DOI: 10.23960/j.hptt.111102-111

Abstract

Bacterial wilt disease caused by Ralstonia solanacearum race 4 is a yield-limiting disease of ginger. A study to investigate the ability of indigenous Arbuscular Mycorrhizae Fungi (AMF) to reduce bacteria wilt disease has been done. This research was designed by using Randomized Block Design (RBD) at greenhouse experiments, using eight AMF isolates with six replication. As supporting data, population R. solanacearum race 4 after treatments was assesed. The results showed that aplication of indigenous AMF  to ginger rhizome as seed treatment dicreased disease incidence, support ginger growth and production such as the seedling formation 150%, plant height 98.6%, the number of leaf 115,7%.  As the result yield of ginger rhizome increased by 400 % and phyloplan 598,3%, respectively, compared to the control.
FORMULASI BAKTERI ENDOFIT AKAR KEDELAI UNTUK PENGENDALIAN PUSTUL BAKTERI Habazar, Trimurti; Resti, Zurai; Yanti, Yulmira; Sutoyo, Sutoyo; Imelda, Imelda
Jurnal Fitopatologi Indonesia Vol 11 No 2 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (458.147 KB) | DOI: 10.14692/jfi.11.2.51

Abstract

Two isolates of bacterial endophyte from soybean root were found to be effective to control bacterial pustule caused by Xanthomonas axonopodis pv. glycines. Formulation of the bacterial isolates is required to maintain the effectivity of this bacterial isolates during storage, transportation and application. The aim of this research was to obtain the best carrier for formulation to maintain the effectivity of bacterial endophyte in storage to control bacterial pustule on soybean. Three kind of carrier agent was evaluated for formulation of bacterial endophyte i.e. peat soil, tapioca flour and coconut water + 1% palm oil. Each carrier agent was combined with 5 treatment of storage time i.e. 0, 1, 3, 5 and 7 weeks. Soybean plants was inoculated by. X. axonopodis pv. glycines 2 weeks after planting by rubbing bacterial suspension (106 cel mL-1) on lower surface of leaves. The results showed that all formulas of bacterial endophyte were able to suppress the bacterial pustule on soybean. The best formulations were bacterial endophyte in peat soil stored for 1 and 7 weeks (effectivity rate 79.85% and 77.02%) and coconut water + palm oil and storaged for 3 weeks (effectivity rate 77.46%).
Penapisan Isolat Rizobakteri dari Perakaran Tanaman Kedelai yang Sehat untuk Pengendalian Penyakit Pustul Bakteri (Xanthomonas axonopodis pv. glycines) Yanti, Yulmira; Habazar, Trimurti; Resti, Zurai; Suhalita, Dewi
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 13, No 1 (2013): Maret, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (246.092 KB) | DOI: 10.23960/j.hptt.11324-34

Abstract

Screening of indigenous  rhizobacteria from healthy soybean root to control bacterial pustule (Xanthomonas axonopodis pv. glycines) using in planta technique. Plant growth promoting rhizobacteria are a group of bacteria  that actively colonize plant roots, increase plant growth and control  plant pathogens. The aim of this study was to obtain rhizobacteri isolates which have the ability to control bacterial pustule and  increase growth and yield of soybean. This method based on in planta selection of enhanced competitive soil root-colonizing bacteria from soil samples of healthy soybean root at endemic area of bacterial pustule in Darmasraya  District  and   Sijunjung District, West Sumatera. We characterized only the best rhizobacteri isolates which have ability to control bacterial pustule and to increase growth and yield of soybean. This type of characterization has possibility to find  new, easy and cheap biocontrol organisms. Ten Rhizobacteri isolates were introduced via seed treatment (108 cfu/ml) and soil drench to 3 week old  soybean seedling. Xanthomonas axonopodis pv. glycines were inoculated to one month old of soybean seedling. The effect of rhizobacteria  on disease incidence, disease severity, plant growth and yield of soybean were evaluated.  We have found that two selected  rhizobacteri isolates from soybean (P12Rz2.1 and P14Rz1.1) were the best isolates in promoting growth and the of the soybean plants  with the effectiveness  20.62 % and 20.47 %.
SKRINING DAN IDENTIFIKASI ISOLAT BAKTERI ENDOFIT UNTUK MENGENDALIKAN PENYAKIT HAWAR DAUN BAKTERI PADA BAWANG MERAH Resti, Zurai; Habazar, Trimurti; Putra, Deddi Prima; Nasrun, .
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 13, No 2 (2013): September, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.607 KB) | DOI: 10.23960/j.hptt.213167-178

Abstract

Screening and identification of endophytic bacteria to control bacterial leaf blight disease on Shallot. The experiment was conducted in Laboratory and Green House, from January to June 2012.   Laboratory experiment consisted of three steps:  (1) isolation of endophytic bacteria from healthy onion roots, (2)In planta /screening of endophytic isolates capable of reducing bacterial leaf blight disease, and (3) molecular identification of potential endophytic isolates.  Treatments of  in planta test were arranged in Completely Randomized Design.  Collected isolates were tested for their capability in controlling bacterial leaf blight disease on shallot.  The variables observed were disease incidence, disease severity, and shallot yield. The results showed that out of 82 isolates successfully isolated, 56 isolates (68.29%) were Gram positive, and 26 isolate (31.71%) were Gram negative. All isolates were HR negative and pathogenicity negative. Six endophytic isolates showed  better performance in inducing resistance and increasing onion yield. Based on 16S rRNA sequence the six isolates were  Bacillus cereus strain P14 , Bacillus cereus strain Se07, Bacillus sp H1, Bacillus sp SJ1 and Serratia marcescens strain PPM4.