Soeharyo Hadisaputro
Bagian Ilmu Penyakit Dalam, Department Epidemiology, Universitas Diponegoro

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IRON STATUS OF NEWBORNS AND UMBILICAL CORD BLOOD HEPCIDIN LEVELS IN GENDER DIFFERENCES Santosa, Qodri; Soemantri, AG; Hadisaputro, Soeharyo; Soejoenoes, Ariawan
Jurnal Kedokteran Brawijaya Vol 31, No 1 (2020)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2020.031.01.12

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Fetal gender might affect the iron status of newborns. Hepcidin has an important role in the process of maternofetal iron transport. This study aims to compare the newborn iron status and the umbilical cord hepcidin levels between male and female gender. A cross-sectional study was conducted with subjects of 84 clinically healthy newborns. Written informed consent and ethical approval were carried out. Newborn iron status observed included (i) hematologic markers (RBC count, Hb, hematocrit, mean corpuscular volume (MCV) and red cell distribution width), and (ii) biochemical markers (serum iron (SI), serum ferritin (SF), soluble transferrin receptor (sTfR) and cord blood hepcidin). Hematologic markers were checked using Sysmex, XN-1000, while Hepcidin and sTfR were using ELISA. Serum iron was checked using IRON Flex®. Statistical analysis was tested with the independent t-test and the Mann-Whitney. All newborns and their mothers were in normal condition. The mean sTfR levels of newborns were significantly higher in the male group than females (38.3±9.06 vs. 34.3±8.16 nmol/L) with p=0.033. High sTfR levels reflect a low iron status. In conclusion, fetal gender differences influence the iron status of newborns, and male newborns have a potentially higher iron deficiency.
The Role of Bamboo Shoot Gigantochloa apus Extract in Decreasing MDA and Increasing IL-10 at The Atherosclerosis Soesanto, Edy; Dharmana, Edi; Hadisaputro, Soeharyo; Muis, Siti Fatimah
Health Notions Vol 3, No 1 (2019): January
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn.v3i1.272

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Introduction: Bamboo shoot Gigantochloa apus extract has antioxidant compounds that act as lipid peroxidation inhibitors and reduce free radical formation so that it can be used as an anti-inflammatory and anti-oxidative stress in the atherosclerosis. Aim: Knowing the effect of bamboo shoot Gigantochloa apus extract in reducing MDA levels and IL-10 increasing levels in rabbits given atherogenic diet. Methods: This experiment used randomized pre-test and post-test with control group design, in 24 New Zealand White rabbits divided into 4 groups randomly. MDA and IL-10 levels were examined by the ELISA method. Results and conclusion: Bamboo shoot Gigantochloa apus extract can reduce MDA levels and increase IL-10 levels significantly in accordance with increasing doses. The increase of MDA levels in the control group with all treatment groups was different (p = 0.0001), and between the treatment groups and other treatment groups there were also differences (p
BEBERAPA FAKTOR YANG BERPERAN TERHADAP KEAKTIFAN KUNJUNGAN LANSIA KE POSYANDU STUDI KASUS DI DESA TAMANTIRTO KECAMATAN KASIHAN KABUPATEN BANTUL PROPINSI DIY Lestari, Puji; Hadisaputro, Soeharyo; Pranarka, Kris
MEDIA MEDIKA INDONESIANA 2011:MMI Volume 45 Issue 2 Year 2011
Publisher : MEDIA MEDIKA INDONESIANA

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ABSTRACTFactors influencing elderly visit to elderly community health care (posyandu lansia): Case study in Tamantirto Village, Kasihan, Bantul, DIYBackground: Elderly community health care (ECHC) is the base of holistic-continuous care for the elderly. The number of elderly at Tamantirto Village is 11.3% of the total residents, which has not been accompanied by a high scope of ECHC visit, where 6 of 9 the ECHCs still have the coverage lower than 50%. Purpose of this study is to prove that age, education, occupation, socio economic status, knowledge, attitude, facilities, access, cadre and health workers services, social role and family role can affect elderly people?s visitations to the ECHC.Methods: Study design: observational analytic, case-control approach. The case: elderly as the ECHC members who actively visited, the control: elderly as ECHC members were who did not actively visit ECHC. The sample?s are 52 cases and 52 controls. The quantitative data were gathered by interviews, while the qualitative data by using focus group disscussion.Results: The factors influencing elderly people?s visitations to the ECHC are age of  ?71 years old (OR:4.6), not working (OR:8.1), good attitude (OR:3), good facilities (OR:5.4), good service of cadres and health workers (OR:6.5) and a good family role (OR:3.2). The factors that do not affect elderly people?s visitations are educational level, socio economic, knowledge, access, and social role ofthe elderly.Conclusion: Characteristics of elderly activity visit ECHC are age of ?71 years old, not working, good attitude, good facilities, good service of cadres and health workers and a good family role.Keywords: Elderly community health care, actively visit, influencing factorsABSTRAKLatar belakang: Posyandu lansia merupakan dasar pelayanan yang holistik dan berkesinambungan pada lansia. Jumlah lansia yang cukup banyak di Desa Tamantirto yaitu sebanyak 11,3% dari jumlah penduduk, belum diiringi dengan cakupan kunjungan yang tinggi, dimana 6 dari 9 posyandu masih mempunyai cakupan di bawah 50%. Tujuan penelitian adalah membuktikan bahwa umur, pendidikan, pekerjaan, kondisi sosial ekonomi, pengetahuan, sikap, fasilitas posyandu, akses terhadap posyandu, pelayanan kader dan petugas kesehatan, peran sosial lansia, serta peran keluarga berpengaruh terhadap kunjungan lansia ke posyandu.Metode: Desain penelitian: analitik observasional, pendekatan kasus kontrol. Kasus adalah lansia anggota posyandu (umur ?60 tahun) yang aktif melakukan kunjungan ke posyandu. Kontrol adalah lansia anggota posyandu (umur ?60 tahun) yang tidak aktifmelakukan kunjungan ke posyandu. Sampel penelitian 52 kasus dan 52 kontrol. Data kuantitatif diperoleh dengan wawancara, data kualitatif dengan diskusi kelompok terarah.Hasil: Faktor yang berpengaruh terhadap keaktifan kunjungan lansia ke posyandu yaitu umur ?71 tahun (OR:4,6), tidak bekerja (OR:8,1), sikap yang baik (OR:3), fasilitas yang baik (OR:5,4), pelayanan kader dan petugas kesehatan yang baik (OR:6,5), peran
PENGARUH PEMBERIAN RUMPUT LAUT SARGASSUM SP TERHADAP KADAR HEMOGLOBIN DAN FERITIN SERUM Yuniarti, Anita; Hadisaputro, Soeharyo; W, Nyoman Suci
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Vol 5, No 1 (2016)
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (424.139 KB) | DOI: 10.14710/jgi.5.1.7-13

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Background : Anemia is a global phenomenon involving developing countries. Fifty percent of anemia cases is caused by iron deficiency. Indonesia is a tropical country that is rich in various kinds of marine plants and still not used optimally.Sargassum sp is one of high iron contains seaweed but still less utilized. Objectives: the aim of this study was to analyze the influence of Sargassum sp on hemoglobin and serum ferritin level. Methods : a simple randomized with pre and posttest control group design was used in this study. Sixteen Wistar male rats were divided into 2 groups (1) control (2) treatment and fed with Sargassum sp 0,49 g for 14 days. During the treatment pre and post hemoglobin and serum ferritin was measured. Results : : iron level in Sargassum sp was 122,37mg/g and Pb level was 0,00009%. In the treatment group hemoglobin level was significantly increased 5,24 mg/dl (p<0,05) and serum ferritin was increased 4,20 µg/ml (p>0,05). Conclusion : sargassum sp increased hemoglobin level on anemic wistar rat but not in serum ferritin significantly. 
RISK FACTORS OF CATARACT IN TYPE 2 DIABETES MELITUS Fauzi, Ahmad; Hadisaputro, Soeharyo
JUKE Unila Vol 4, No 8 (2014)
Publisher : Fakultas Kedokteran Universitas Lampung

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Abstract

Background: One of Diabetes Melitus (DM) complication to the eye that may result as visual disturbances and blindness is cataract. According to The Framingham Eye Study and the Health and Nutrition Examination Survey shows that patient with DM before age of 65 years is easier 3-4x to get cataract and 2x easier than the age after 65 years compared to non DM. The research about risks factors for diabetic cataract in type 2 DM in Indonesia is rare, so that it is necessary to do the research about these risk factors. Method: This study involves observational analytic study with case control study design. Subject of the case is type 2 DM with cataract (73 people) and type 2 DM without cataract as control (73 people) at Dr. Kariadi Hospital Semarang. Then data followed retrospectively about risk factors that influence incidence of cataract in type 2 DM. The stages of analysis used in this study is univariate analysis, bivariate analysis and multivariate analysis. The statistics test used in multivariate analysis is Multiple Logistic Regression. Result: The multivariate analysis shows that 5 years Duration of DM or longer (P=0,002) and Trigliserida value ≥150 mg/dl (P=0,015) are the risks factors for cataract in type 2 DM. Conclusion: 5 years duration of DM or longer and trigliserida value ≥150 mg/dl are factors that proven as risk factors for cataract in type 2 DM. [Juke Unila 2014; 4(8):173-179]
SUMBANGAN ALL-TRANS ASAM RETINOAT (ATRA) BAGI PENYEMBUHAN PERIODONTITIS Praptiwi, Praptiwi; Muis, Siti Fatimah; Hadisaputro, Soeharyo; Suryono, Suryono
MEDIA MEDIKA INDONESIANA 2011:MMI Volume 45 Issue 3 Year 2011
Publisher : MEDIA MEDIKA INDONESIANA

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Abstract

ABSTRACTAll-trans retinoic acid (ATRA) contribution to periodontitis healingBackground: In inflammation and infection of periodontal ligament i.e periodontitis, collagen fiber is damaged. All-trans retinoic acid (ATRA), metabolite of vitamin A, has potency to depress damage in inflammation. Indonesia with high vitamin A deficiency, facing to delayed recovery of periodontitis. The disease is vastly suffered by common people in this country. The study aimed to know the collagen status of periodontal ligament suffering periodontitis treated with tetracycline and ATRA supplementation.Method: The experimental study using post test only design in 12 adult male Wistar rat was done in 7 days. The animal study were induced periodontitis through Porphyromonas gingivalis inoculation. Tetracycline 90 mg/kg was given, beside ATRA supplementation of 10 mg/kg and 5 mg/kg. Four experimental groups were chosen randomly. Group of periodontitis + tetracycline was the control of the study. The independent variables were ATRA supplementation of those high and low dose. The dependent variable was collagen status. Collagen status consist of degradation and synthesis of collagen, indicated by mRNA MMP-2 and mRNA P1CP respectively. They were measured by reversed transcriptase polymerizing chain reaction and thin layer chromatography. All-trans retinoic acid concentration was measured using high performance liquid chromatography. The dataanalyzed using t-test, Oneway ANOVA, post hoc duncan and least significant differences.Result: In group receiving 10 mg ATRA/kgBB, the ATRA level was highest but the collagen degradation was lower although not significant compared to the low dose ATRA group. In both groups the degradation was significantly lower than the control group (p=0.007). The synthesis of collagen in the low dose ATRA group was higher than the high dose ATRA group and the control group.Conclusion: High dose of ATRA supplementation contribution to less degradation of collagen, but in term of collagen synthesis, low dose ATRA supplementation gives better result.Keywords: ATRA supplementation, collagen degradation, periodontitis ABSTRAKLatar belakang: Pada peradangan dan infeksi kronik ligamentum periodontal yaitu periodontitis, terjadi kerusakan serat kolagen. All-trans asam retinoat (ATRA), metabolit vitamin A, berpotensi menekan kerusakan pada peradangan. Tingginya defisiensi vitamin A di Indonesia, menghambat proses penyembuhan periodontitis yang banyak diderita masyarakat luas. Penelitian bertujuan mengetahui status kolagen ligamentum periodontal periodontitis yang mendapat pengobatan tetrasiklin dan suplementasi ATRA.Metode: Studi eksperimental dengan rancangan post test only pada 12 tikus Wistar jantan dewasa telah dilakukan selama 7 hari. Hewan coba diinduksi periodontitis melalui inokulasi bakteri Porphyromonas gingivalis. Diberikan tetrasiklin 90 mg/kg, juga suplementasi ATRA 10 mg/kg dan 5 mg/kg. Empat kelompok studi dipilih secara acak. Kontrol adalah kelompok sakit + tetrasiklin. Variabel bebas adalah suplementasi ATRA dosis tinggi dan dosis rendah, variabel tergantung adalah status kolagen. Status kolagenmencakup degradasi dan sintesis, berturut-turut dengan petanda mRNA MMP-2 dan mRNA P1CP, diukur menggunakan reverse transcriptase polymerizing chain reaction dan thin layer chromatography. Kadar ATRA diukur dengan high performance liquid chromatography. Data dianalisis dengan uji t, Oneway ANOVA, post hoc Duncan dan least significant differences.Hasil: Pada kelompok yang menerima suplementasi ATRA 10 mg/kgBB kadar ATRA tertinggi, tetapi degradasi kolagennya lebih rendah walau tidak bermakna. Kedua kelompok sumplementasi degradasi kolagennya lebih rendah secara bermakna dibanding kelompok kontrol (p=0,007), dalam sintesis kolagen,kelompok yang mendapat suplementasi ATRA 50 mg/kgBB sintesisnya lebih baik dibanding kelompok yang mendapat ATRA 10 mg/kgBB maupun kelompok kontrol.
HUBUNGAN ASUPAN SENG, VITAMIN A, DAN STADIUM KLINIS TERHADAP STATUS GIZI DAN JUMLAH CD4+ PADA ANAK TERINFEKSI HIV DI WILAYAH KOTA DAN KABUPATEN SEMARANG Margareth, Waisaktini; Hadisaputro, Soeharyo; Margawati, Ani
Media Gizi Mikro Indonesia Vol 10 No 1 (2018): Media Gizi Mikro Indonesia Desember 2018
Publisher : Balai Penelitian dan Pengembangan Kesehatan Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/mgmi.v10i1.594

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Latar Belakang. Peningkatan jumlah kasus infeksi HIV anak di Indonesia paralel dengan peningkatan persentase transmisi penularan AIDS dari ibu ke anaknya, dari 3 persen (2013) menjadi 4,6 persen (2015). Salah satu tujuan pemberian terapi antiretroviral (ARV) pada kasus HIV anak adalah untuk meningkatkan jumlah sel T-CD4+. Semakin berat stadium klinisnya akan menurunkan kadar CD4+. Pemberian suplementasi zat gizi mikro dapat meningkatkan status gizi penderita HIV anak yang menjalani pengobatan ARV. Tujuan. Penelitian ini bertujuan menganalisis hubungan asupan seng, vitamin A, dan stadium klinis infeksi HIV terhadap status gizi dan jumlah CD4+ pada kasus HIV anak di Kota dan Kabupaten Semarang. Metode. Penelitian ini menggunakan desain cross-sectional. Subjek penelitian adalah anak yang menderita HIV berumur 1-14 tahun sebanyak 31 subjek. Data yang dikumpulkan meliputi data tinggi badan (TB), berat badan (BB), asupan zat gizi yang diperoleh dengan metode food recall 2x24 jam. Jumlah CD4+ diukur melalui pemeriksaan darah subjek. Data dianalisis menggunakan uji chi-square dan regresi logistik untuk menghitung Prevalence Rasio (PR). Hasil. Hasil penelitian ini menunjukkan bahwa asupan seng memberikan risiko bermakna terhadap kejadian berat badan rendah (PR=3,020; p=0,029; CI=1,043-8,739). Asupan vitamin A memberikan risiko bermakna terhadap rendahnya kadar CD4+ (PR=3,036; p=0,021; CI=1,211-7,608 dan PR=2,8; p=0,018; CI=1,331-5,891). Stadium klinis tingkat sedang memberikan risiko bermakna terhadap rendahnya kadar CD4+ rendah (PR=8,211; p = 0,004; CI=1,227-54,962). Probabilitas jumlah CD4+ rendah ketika penderita pada stadium klinis infeksi HIV berat sebesar 14,3 persen. Kesimpulan. Stadium klinis sedang-berat meningkatkan risiko terjadinya penurunan jumlah CD4+ di dalam sel-T (&lt;500sel/mm3).
Kadar Oksidan yang Tinggi Sebagai Faktor Risiko Terjadinya Hemolisis pada Neonatus Sepsis R, Kamilah Budhi; Aminullah, Asril; Hadisaputro, Soeharyo; Soemantri, Ag; Suhartono, Suhartono
Sari Pediatri Vol 14, No 3 (2012)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (90.352 KB) | DOI: 10.14238/sp14.3.2012.198-204

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Latar belakang. Sepsis merupakan penyebab utama morbiditas dan mortalitas neonatus. Penyebab hemolisis pada neonatos meliputi: fisiologis, proses imun, stres oksidatif, aktivasi komplemen, kelainan eritrosit, enzim hemolisin. Penyebab hemolisis pada neonatus sepsis belum banyak diteliti. Tujuan. Membuktikan bahwa kadar oksidan (MDA) yang tinggi sebagai faktor risiko terjadinya hemolisis pada neonatus sepsis.Metode. Penelitian di RS Dr. Kariadi, Semarang tahun 2009, desain observasional prospektif dengannested case – controlpada 94 neonatus sepsis terdiri 47 kelompok kasus (hemolisis positif ) dan 47 kontrol (hemolisis negatif ). Diagnosis sepsis ditegakkan dengan kriteria SIRS (systemic inflammatory response syndrome)1 atau lebih, gejala klinik, pemeriksaan laboratorium atau penunjang lain. Hemolisis ditegakkan dengan metode indeks retikulosit >3, hari ke-1 dan ke-3. Pemeriksaan faktor risiko kadar MDA, GPx dengan metode spektrofotometri, vitamin C dengan metode colorimetric assay, vitamin E dengan metode ELISA, hemolisin dengan kultur darah media agar darah. Uji hipótesis menggunakan Chi-square, OR (95% Cl), Mantel-Haenszeldan regresi logistik.Hasil. Kejadian hemolisis pada neonatus sepsis 49%. Kadar MDA kelompok kasus (5,3±2,06) lebih tinggi bermakna dibanding kelompok kontrol (3,3±1,27) p=0,0001. Analisis bivariat, kadar MDA tinggi (>2,90 ng/dL) merupakan faktor risiko hemolisis pada neonatus sepsis (OR 11,6; 95% CI 2,5-54,1) Analisis multivariat, kadar MDA tinggi (> 2,90 ng/dL) dengan memperhitungkan interaksi GPx (OR 5,16; 95%CI 1,22-21,86), vitamin E (OR 5,77; 95%CI 1,49-22,26) dan vitamin C (OR 11,26:2,38-53:30) merupakan faktor risiko kejadian hemolisis pada neonatus sepsis. Hemolisin belum dapat dibuktikan Kesimpulan. Kadar oksidan (MDA) yang tinggi (>2,90 ng/dL), merupakan faktor risiko terjadinya hemolisis pada neonatus sepsis.
STUDI EFFEKTIVITAS KEFIR BENING DAN MENIRAN (Philanthus niruri) TERHADAP PENURUNAN KADAR GLUKOSA DARAH PADA HEWAN TIKUS WISTAR HIPERGLIKEMIA DIINDUKSI STREPTOZOTOCIN ., Judiono; Hadisaputro, Soeharyo; Djokomoeljanto, RRJ; Laksono, Budi; W, Theophilus
GIZI INDONESIA Vol 33, No 2 (2010): September 2010
Publisher : PERSATUAN AHLI GIZI INDONESIA

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Abstract

EFFECTIVITY STUDY OF PLAIN KEFIR AND MENIRAN (PHILANTUS NIRURI) ON BLOOD GLUCOSE LEVELS ON HYPERGLYCEMIA WISTAR RATS INDUCED STREPTOZOTOCINTraditional medicines and functional foods used for diabetes therapy increased in Indonesia. Bioactive components play an important role in decreasing blood glucose. The study aims to prove the effectiveness of plain kefir and Meniran (Philanthus niruri) to decrease blood glucose levels in wistar rats with streptozotocin-induced hyperglycemia. The study design was randomized pre – post test control group. A number of 60 male rats aged 2.5-3 months, 150-250 g were made hyperglycemia by induced streptozotocin (STZ) 40 mg / kg berat badan. The hyperglycemic rats were grouped into: (1) treated with 0.76 UI insulin /day, (2) treated with 3.6 mL plain kefir /day, (3) treated 2.7 mL meniran / day, (4) positive control group of hyperglycemic rats, and (5) negative control group (standard diet ad libitum). The ratswere given feed refers to the AIN 93. Fasting glucose levels before and after treatment were measured by Super (Glucocard II) enzimatic method. The results showed that body weight increased in all treatments, except meniran, however the increaments were not differ significantly. Changes in body weight of insulin group were 13.800 + 16.104 g, kefir treatment amounted to 13.812 + 21.294, and meniran increased about 18.394 + 16.225. Changes in blood glucose in the insulin group about -118.571+55.815 mg/dL, kefir treatment at -102.875+60.454, while meniran group -66,625+37,784. In contrast, the positive control group tended to increase glucose levels. Conclusion In vitro, plain kefir and meniran potentially lowered blood glucose levels. However, kefir showed lowered glucose level better than meniran. Futher research needs to study bio- molecular mechanisms of the decline in the future.Key words: diabetes mellitus, meniran, plain kefir, streptozotocin (STZ), hyperglycemic rat
FAKTOR YANG BERPENGARUH TERHADAP KEJADIAN DEMAM BERDARAH DENGUE PADA ANAK USIA 6-12 TAHUN Sandra, Tuti; Sofro, Muchlis AU; Suhartono, Suhartono; Martini, Martini; Hadisaputro, Soeharyo
Jurnal Ilmiah Permas: Jurnal Ilmiah STIKES Kendal Vol 9 No 1 (2019): Januari
Publisher : LPPM STIKES KENDAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (101.672 KB) | DOI: 10.32583/pskm.9.1.2019.28-35

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Demam Berdarah Dengue (DBD) adalah penyakit infeksi yang masih menjadi masalah kesehatan yang serius karena bersifat endemis. Penyebaran di daerah tropis dan subtropis terutama di Asia Selatan-Timur, Asia Tenggara. Penelitian ini bertujuan untuk menjelaskan beberapa faktor yang berpengaruh terhadap kejadian DBD pada anak usia 6-12 tahun. Penelitian menggunakan desain penelitian campuran atau mixed methode yang mengkobinasikan bentuk kuantitatif dan kualitatif. Spesifikasi penelitian kuantitatif :observasional analitik desain case control study. Dilakukan kajian kualitatif dengan wawancara mendalam. Populasi studi adalah semua anak usia 6-12 tahun yang tinggal di Kota Semarang. Jumlah subyek 70 kasus dan 70 kontrol yang diambil secara consecutive sampling. Data di analisis secara bivariat dan multivariat dengan metode regresi logistik. Faktor-faktor &nbsp;yang terbukti berpengaruh terhadap kejadian DBD pada anak usia 6-12 tahun; pendidikan ibu rendah (p= 0.004; OR &nbsp;3.031; 95%CI 1.4281-6.434), kebiasaan tidak memakai obat anti nyamuk ( p= 0.001; OR 4.293; 95%CI 1.935-9.526), kebiasaan tidak memakai pakaian panjang (p= 0.013; OR 2.759; 95%CI 1.240-6.138) Sedangkan faktor yang terbukti tidak berpengaruh; kebiasaan tidur siang, kebiasaan menggantung pakaian dalam rumah, forum penyampaian informasi, rutinitas pemeriksaan jentik dan praktik PSN. Faktor-faktor yang terbukti berpengaruh terhadap kejadian DBD pada anak usia 6-12 tahun adalah pendidikan ibu rendah, kebiasaan tidak memakai obat anti nyamuk dan kebiasaan tidak memakai pakaian panjang memiliki probabilitas 85,3% untuk mengalami DBD. Kata kunci&nbsp; : DBD, nyamuk, dengue. &nbsp; VARIOUS FACTORS INFLUENCING THE DENGUE FEVER OCCURRENCES IN THE CHILDREN AGE 6 TO 12 YEARS OLD &nbsp; ABSTRACT The Dengue Fever is an infectious disease that still becomes a serious health issue since it is endemic. The disease spreads in the tropical and subtropical areas particularly in the Southeast Asia. This research aims to explain the various factors influencing the occurrences of the dengue fever in the children age 6 to 12 years old. This research uses mixed method research design combined with both quantitative and qualitative methods. The quantitative research specification is the observational analytic case control design while the indept interview technique is used as the qualitative research method. The population of the study is all children age 6 to 12 years old who live in the Semarang City. The number of the subjects is 70 cases and 70 controls which are taken by consecutive sampling method. The data are analyzed using the bivariate and multivariate with the regression logistic method. The factors that has been proven to influence the dengue fever occurrences in the children 6 to 12 years old are the lack of the mother?s education ((p= 0.004; OR &nbsp;3.031; 95%CI 1.4281-6.434), the habit of not using the insect repellent use (p= 0.001; OR 4.293; 95%CI 1.935-9.526) and the habit of not wearing long clothes (p= 0.013; OR 2.759; 95%CI 1.240-6.138). Meanwhile, the habit of taking nap, the habit of hanging clothes, the information delivery forum, the routine examination of the mosquitos? larva and the mosquitos? eradication are not shown as the influential factors causing the dengue fever. The factors recognized as the significant factors against the occurrences of the dengue fever in the children age 6 to 12 years old are the lack of the mother?s education, the habit of not using the insect repellent and the habit of not wearing long clothes have the probability 85,3% to experience the dengue fever. Keywords : Dengue Fever, Mosquito, Dengue