HAMIM HAMIM
Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor

Published : 22 Documents
Articles

Found 22 Documents
Search

ENKAPSULASI DAN REGENERASI KALUS EMBRIOGENIK MANGGA (MANGIFERA INDICA L.) KULTIVAR BAPANG DAN GADUNG 21 Ermayanti, Tri Muji; Nugroho, Robertus; Hamim, Hamim
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2598

Abstract

The mother plant and genetic variability of Indonesian mango need to be conserved. Encapsulation is one of in vitro conservation used for many plant species. The aim of this research was to study the regeneration of encapsulated mango cultivar Bapang and Gadung 21 embryogenic callus after storage at -14, 3?5, and 26?27oC for 0, 1, 2, 4, and 8 weeks. Embryogenenic callus was treated with 3% Na-alginate (in liquid 3M), then it was dropped into 100 mM CaCl2. Encapsulated callus beads were dehidrated and stored at -14, 3?5, and 26?27oC for 0, 1, 2, 4, and 8 weeks. After storage, the callus was cultured in 3M medium containing 2,4-D at 0, 1, and 2 mg/l for the regeneration. The results showed that after 8-week storage, callus of Bapang culticar was more viable (67.3%) and resulted more number of somatic embryos (191.6%) than Gadung 21 cultivar. The callus which was cultured in 3M medium without addition of 2,4-D was more viable (20.9%) and had more number of somatic embryos (1062.5%) than that which was cultured on medium containing 2,4-D. After 2-week storage, callus had viability of 7.6%. No storage callus formed more somatic embryos than storage callus. Storage at 26?27oC gave higher viability as well as higher number of somatic embryo than stored at -14 and 3?5 oC. The callus did not regenerate into shoots after storage at -14oC. Embryogenic callus could be stored at 3?5 and 26?27oC for 4 weeks.
REKAYASA EKSPRESI GEN PEMBUNGAAN Hd3a DIBAWAH KENDALI PROMOTER ROL C PADA JARAK PAGAR (Jatropha curcas L.) Sulistyaningsih, Yohana C; Hartana, Alex; Widyastuti, Utut; Hamim, Hamim; Suharsono, Suharsono
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (500.374 KB) | DOI: 10.14203/beritabiologi.v10i6.1940

Abstract

Flowering in jatropha (Jatropha curcas L.) was considered as one of major factors that contribute to its productivity. Small number of female flowers produced in each inflorescence was believed as the main cause of low seed production.Introduction of Hd3a flowering gene driven by rol C promoter was supposed to improve total number of flowers including female flower.The objective of this research was to optimize cell proliferation and regeneration medium in Jatropha transformation method mediated by Agrobacterium, to obtain transgenic Jatropha containing Hd3a flowering gene as well as to understand the effect of this transgene on jatropha flowering character.Callus induction medium containing 0.5 mg/1 IBA added with 3 g/1 PVP produced the highest frequency of shoot formation.We obtained 26.67% to 33.33% putative transgenic plantlets that were able to grow in 40mg/l hygromycin selection medium. PCR analysis revealed that seven out of 10 putative transgenic plantlets were positively transgenic.Extremely early flowering character that was confirmed by histological analysis was also shown by some transgenic plantlets.
PHOTOSYNTHESIS OF C3 AND C4 SPECIES IN RESPONSE TO INCREASED CO2 CONCENTRATION AND DROUGHT STRESS HAMIM, HAMIM
HAYATI Journal of Biosciences Vol. 12 No. 4 (2005): December 2005
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.934 KB) | DOI: 10.4308/hjb.12.4.131

Abstract

Photosynthetic gas exchange in response to increased carbon dioxide concentration ([CO2]) and drought stress of two C3 (wheat and kale) and two C4 species (Echinochloa crusgallii and Amaranthus caudatus) were analysed. Plants were grown in controlled growth chambers with ambient (350 ?mol mol-1) and doubled ambient [CO2]. Drought was given by withholding water until the plants severely wilted, whereas the control plants were watered daily. Even though stomatal conductance (Gs) of C4 species either under ambient or double [CO2] was lower than those in C3, doubled [CO2] decreased Gs of all species under well watered conditions. As a result, the plants grown under doubled [CO2] transpired less water than those grown under ambient [CO2]. Photosynthesis (Pn) of the C4 species was sustained during moderate drought when those of the C3 species decreased significantly. Doubled [CO2] increased photosynthesis of C3 but not of C4 species. Increased [CO2] was only able to delay Pn reduction of all species due to the drought, but not remove it completely. The positive effects of increased [CO2] during moderate drought and the disappearance of it under severe drought suggesting that metabolic effect may limit photosynthesis under severe drought.
KARAKTERISTIK PERTUMBUHAN JARAK PAGAR (Jatropha curcas L.) YANG DITAMBAHKAN CENDAWAN ENDOFIT PADA LAHAN PASCA TAMBANG TIMAH Gedoan, Sukmarayu P.; Hartana, Alex; Hamim, Hamim; Widyastuti, Utut; Sukarno, Nampiah
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol 3, No 1 (2013): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Graduate School Bogor Agricultural University (SPs IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.3.1.10

Abstract

Tin mining activity caused canging in physical and chemical characteristic of the soil that were not suitable for growth of plants. The objective of this experiment was to study accessions of Jatropha curcas planted on post tin mining land which were given endophyte. This research was conducted in a Sinar Baru village TS 133, district of Bangka, Bangka Belitung province for field research conducted in May 2007 to April 2008. The experimentas a factorial experiment in the design of the randomized complete block design with three replications. The main plot is 7 accessions consisting of: accession Madiun, Ponorogo, Jember, Dompu, Lampung, Bengkulu, andSukabumi, while the subplot of the land without giving endophyte fungal (control) and the provision of endophyte fungal in baglog 250 g. Each experimental unit contained four plants per plot.The result showed that vegetative growth the highest for the former tin mining land given endophyte fungal vary in some accessions. Accession to the highest Sukabumi: plant height, branch number, plant dry weight, dry weight of the shoot, and root dry weight, the largest diameter have Jember accession, accession Dompu had the highest canopy diameter, and the accession of Lampung has the lowest ratio of shoot roots.Keywords: Jatropha curcas, accession, fungal endophyte
PERTUMBUHAN TANAMAN JARAK PAGAR (JATROPHA CURCAS L.) PADA LAHAN PASCA TAMBANG TIMAH DI BANGKA YANG DIBERI PUPUK ORGANIK Gedoan, Sukmarayu P; Hartana, Alex; Hamim, Hamim; Widyastuti, Utut; Sukarno, Nampiah
JURNAL ILMIAH SAINS Volume 11 Nomor 2, Oktober 2011
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jis.11.2.2011.205

Abstract

PERTUMBUHAN TANAMAN JARAK  PAGAR (Jatropha curcas L.) PADA LAHAN PASCA TAMBANG TIMAH DI BANGKA YANG DIBERI PUPUK ORGANIK Sukmarayu P. Gedoan1), Alex Hartana2), Hamim2), Utut Widyastuti2) dan Nampiah Sukarno2) 1)Program Studi Biologi, FMIPA Universitas Negeri Manado di Tondano; 2)Departemen Biologi, FMIPA, Institut Pertanian Bogor, Bogor 16680   ABSTRAK Kegiatan penambangan timah menyebabkan perubahan karakteristik fisika dan kimia tanah sehingga menjadi tidak sesuai untuk pertumbuhan tanaman.  Tujuan penelitian ini untuk mempelajari pertumbuhan 7 aksesi jarak pagar yang ditanam pada lahan pasca tambang timah yang diberi kompos dan kotoran sapi.  Penelitian ini dilakukan di TSS 133, Kelurahan Sinar Baru, Kabupaten Bangka, Provinsi Bangka Belitung.  Penelitian lapangan dilaksanakan pada Bulan Mei 2007 sampai dengan Bulan Desember  2008.  Percobaan faktorial ini dirancang dalam Rancangan Petak Terbagi dengan tiga ulangan.  Petak utama adalah 7 aksesi jarak pagar yang terdiri atas: aksesi Madiun, Ponorogo, Jember, Dompu, Lampung, Bengkulu,  dan Sukabumi, sedangkan  anak petak berupa tanah tanpa pemberian kompos dan pupuk kandang (kontrol), kompos trubus 4 kg/lubang ditambah 4 kg tanah bagian atas  dan  kotoran sapi 4 kg/lubang ditambah 4 kg tanah bagian atas. Hasil penelitian menunjukkan bahwa pertumbuhan awal yang paling baik untuk tanah bekas tambang timah adalah pada aksesi Jember yang ditunjukkan oleh diameter batang, berat kering tanaman, berat kering tajuk dan berat kering akar.  Sedangkan tinggi tanaman tertinggi diamati pada aksesi Madiun.  Penambahan kotoran sapi dapat meningkatkan produksi biji dan kandungan minyak.  Produksi biji tertinggi diperoleh pada aksesi Bengkulu dan kandungan minyak tertinggi diperoleh pada aksesi Dompu. Kata kunci: aksesi, Jatropha curcas, kompos, kotoran sapi   THE GROWTH OF CASTOR OIL PLANT (Jatropha curcas L.) ON THE POST-TIN-MINING LAND IN BANGKA PROVIDED WITH ORGANIC FERTILIZER ABSTRACT The activity of tin mining changes the physical and chemical characteritics of soil, so that the soil isnot suitable for plant growth.  The objective of this research was to evaluate the growth of some accession of Jatropha curcas that we planted on post-tin-mining land provided with compost and cow feces.   This research was conducted in TS 133, Sinar Baru Village, District of Bangka, Province of Bangka Belitung.  This field research was carried out in May 2007 to December 2008.  Factorial experiment was designed as split plot with three replications.  The main plot was 7 accessions, i.e. Madiun, Ponorogo, Jember, Dompu, Lampung, Bengkulu, and  Sukabumi.  The subplot waskonds of organic fertilizer, i.e. top soil without addition of compost and cow fecer (control), trubus compost  (4 kg/hole) added with top soil (4 kg/hole), and cow feces (4 kg/hole) added with topbsoil (4 kg/hole). The result showed that Jember accession had the best early growth based on the observation of stem diameter, plant dry weight, and root dry weight.  The largest plant height was observed in Madiun accession.  The higest seed production was observed in Bengkulu accession and Dompu accession had the higest oil content.Keywords: accession, Jatropha curcas, , compost, cow feces
STRATEGI KOMUNIKASI PUBLIC RELATIONS DINAS KOMUNIKASI DAN INFORMATIKA PROVINSI JAWA TIMUR UNTUK MENINGKATKAN CITRA DI MATA PUBLIK Mardiana, Wike; Hamim, Hamim; Widiyanto, Kendry
JURNAL REPRESENTAMEN Vol 3, No 02 (2017)
Publisher : Universitas 17 Agustus 1945 Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30996/.v3i02.1415

Abstract

The purpose of this study is to provide an overview of communication strategy of PublicRelations that executed by DinasKomunikasidanInformatika of East Java Province in order toimprove positive image according to Two-Way Symetric theory and supporting theory by HaroldLasswell. The writer applies descriptive qualitative method in compiling this thesis. DinasKomunikasidanInforatika of East Java Province. The purpose of this study is to explain anyphenomenon in details by specific data collection. This study describing communication strategy ofPublic Relations that executed by DinasKomunikasidanInformatikaof East Java Provience. The resultof study shows that strategy executed by DinasKominfo to improve their image in front of public areby creating website, creating facebook account, and creating twitter account in order to ease public inmaking any complain and to ease public finding any information. Public Relations DinasKominfousing two approaching strategy including persuasive and educative approaching, and cooperationapproaching with internal public and external public.Keywords : Public Relations Strategic, Dinas Komunikasi dan Infromatika Provinsi Jawa Timur,Image.
ANALISIS INTERAKSI SIMBOLIS BATU AKIK BACAN DI KALANGAN MASYARAKAT SURABAYA) Ningsih, Sri Wahyu; Hamim, Hamim; Soenarjanto, Bagus
JURNAL REPRESENTAMEN Vol 2, No 01 (2016)
Publisher : Universitas 17 Agustus 1945 Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30996/.v2i01.1454

Abstract

Research agate phenomenon as a symbol of this communication in the background agate background bythe icon as a symbol of social status differentiation in terms of both wealth and culture as well. Problemsof this research is the type of agate Bacan able to become a symbol of communication amongmasyarakat.Penelitian aims to determine the message symbols in agate frequently in associate withwealth and status sosial.penelitian using qualitative research The results of the study show that thesymbol is Non-verbal manifestation of a culture intent contained therein, as a symbol of social statusdifferentiator differentiator as well as cultural diversity. In relation to the communication titlephenomenon agate as a symbol of communication close relation to verbal communication which refers tospeech and non-verbal communication can be the symbol of the emblem as violence rocks / scale mohsand also fiber stone, whereas the results of research by the author intervenes after the interview is fromsome informants one of academics statement on responsible is in accordance with the theory of symbolicinteraction that communication tidah only through language but there are symbols that agreed upon inaddition to non-language and theories of social behavior by Albert Bandura that the individual isinfluenced by the social environment in if the surrounding environment fond agate then that individualwill have a tendency to follow the social life also has agate want.Keywords : Symbolic interaction, culture, agate Bacan.
Respon Pertumbuhan Spesies C3 dan C4 terhadap Cekaman Kekeringan dan Konsentrasi CO2 tinggi Hamim, Hamim
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 3 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.3.174

Abstract

Growth response of two C3 (wheat and kale) and two C4 species (Echinochloa crusgallii and Amaranthus caudatus) were analyzed in response to drought stress under different CO2 concentrations, i.e. ambient (350 ppm) and double ambient (700 ppm).  Drought was given by withholding water for several days until the plants were wilted.  Drought stress reduced relative water content (RWC), water potential (Yw), osmotic potential (Ys), net photosynthesis (Pn) and plant growth of all species.  During moderate drought Pn of C3 species decreased considerably, while that of C4 species was still sustained, suggesting that C4 cycle is important to sustain Pn during the beginning of the drought.  Doubled [CO2] enhanced growth of all species indicated by increase of plant height and leaf area, but the growth of C3 species was higher than that of C4 one.  Increase of growth due to high [CO2] was associated with an increase of Pn in C3 species but not in C4 species.  High [CO2] caused C4 species had RWC, Yw and Ys higher than C3 species during drought stress.  Increase of growth in C4 species may be associated by an increase of plant water status due to high [CO2].    
PENCITRAAN BUPATI DALAM PERSPEKTIF SEMIOTIKA BARTHES Analisis Penciitraan Bupati Sidoarjo Melalui Iklan Apartemen Royal Mutiara Residence pada Jawa Pos Novianto, Tonny; Hamim, Hamim; Novaria, Rachmawati
JURNAL REPRESENTAMEN Vol 2, No 01 (2016)
Publisher : Universitas 17 Agustus 1945 Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30996/.v2i01.1459

Abstract

Research starts from the interest of writer of the advertising world displayed through theprintable media especially Apartment Royal Mutiara Residence commercial in Jawa Pos, May 2015thedition. This commercial provides persuasive messages are presented in the textual message form andthe image message form. In this commercial there is a picture of Sidoarjo’s Regent H SaifulIllah,S.H.,M.Hum which is have a multi-interpretation in society as if this commercial have twoobjectives that mutually contrary, first, this commercial is intended to introduce the latest products ofApartment Royal Mutiara Residence, the second, this commercial also has a hidden meaning inconstructing image of Sidoarjo’s Regent H Saiful Illah, S.H.,M.Hum. This study adopted a qualitativeapproaches.. In this case, writers strove to unload the denotative meaning, connotative meaning andmyths which is found in verbal message and non verbal message that appear in the Apartment RoyalMutiara Residence commercial in Jawa Pos, May 2015th edition. From this research, can beconcluded that purport decker approach in Roland Barthes semiotic analysis, through connotativemeaning, denotative meaning and myths in this commercial, identified the explicit meaning ofpersuasive messages that appeare in this commercial aimed to construct the image of Sidoarjo’sregent H Saiful Illah, S.H., M.Hum to be much more positive in society.Keywords : Commercial, Semiotic, Image, Regent, Roland Barthes
SIMBOL KOMUNIKASI PEDAGANG KELILING DI SIDOSERMO SURABAYA Pratiwi, Bertharia Sanny; Hamim, Hamim; Wibowo, Judhi Hari
JURNAL REPRESENTAMEN Vol 2, No 02 (2016)
Publisher : Universitas 17 Agustus 1945 Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30996/.v2i02.1397

Abstract

This research was motivated by the communication symbol or symbols likethis is very attached to the Indonesian nation and even many people who are not awarethat it was applying the theory of communication in their daily lives. Emblem usefulnessas a tool to influence the communicant, a tool to make someone understand the messagedelivered, the tools to make sense of the messages being delivered, means to connect thecommunicator to the communicant, a tool to achieve a goal of communication. The soundof street vendors around the place we live, with a characteristic sound when the merchantwas around the area where you live to peddle his wares. Pedlar or commonly referred toPKL is the term given to the hawkers who use carts.As for supporting this study, the authors in the manufacture thesis usingqualitative methods deskripstif. Goal authors is that the notion of communication Symboltraders we need to understand more, because a lot of symbols - symbols of merchants thatwe do not understand.Keywords : symbol communication , trader