Amelia Hana
Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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RESPONS HEMATOLOGI DAN KIMIA DARAH DOMBA LOKAL INDONESIA TERHADAP STRES TRANSPORTASI SELAMA 12 JAM (HAEMATOLOGICAL AND BLOOD BIOCHEMICAL RESPONSES TO 12 HOUR TRANSPORTATION STRESS IN IN LOCAL INDONESIAN SHEEP) Sarmin, Sarmin; Hana, Amelia; Astuti, Pudji; Febrianto, Yuda Heru; Airin, Claude Mona
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.246 KB) | DOI: 10.19087/jveteriner.2019.20.1.48

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The objective of this study was to investigate the effect of transport 12 hours on  haematological and some biochemical responses of local Indonesian sheeps.  Ten adults (2.0-2.5 years)  male local Indonesian sheeps  BW adult 30-40 kg were transported by L-300 pickup truck starting at 3:00 pm to 3:00 am on the d 2 without stopping. Jugular blood were collecte-d at 7 am (-8 h pre- transportation as baseline), 15:00  immadiately after (0 h),  19:00 (4 h), 23:00 (8 h) ,  03.00 (12 h ), and at 11.00 d 2 (+8 h)  post-  transportation as recovery period). Under  the conditions of the present study, transport of local Indonesian sheeps significantly increased glucose  during 4 h  transport (P <0.05), increased eosinophil at 0 h (P <0.05) and no significant changed in erythrocytes, haemoglobin , monocytes, neutrophils, lymphocytes, leukocytes,  packed cell volume (PCV), and total plasma protein (TPP) (P> 0.05). Our findings showed that transportation 12 h affected  eosinophil during 0 h and glucose metabolism during 4 h of transportation, but did not any affect on some haematology and dehydration responses in local Indonesian sheeps. 
KAJIAN KADAR KORTISOL SAPI YANG DIPOTONG DI RUMAH POTONG HEWAN YOGYAKARTA S, Sarmin; Hana, Amelia; Heru Fibrianto, Yuda; Mona Airin, C.
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.034 KB) | DOI: 10.21157/j.ked.hewan.v8i2.2635

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Penelitian ini bertujuan mengkaji tingkat stres sapi yang dipotong di Rumah Potong Hewan (RPH) Yogyakarta. Dalam penelitian ini digunakan 20 ekor sapi yang dibagi menjadi dua kelompok, yakni kelompok sapi yang dilakukan restraint dengan restraining box tipe Mark 1 (K1, n=13) dan kelompok sapi yang dilakukan restraint dengan metode konvensional (K2, n= 7). Masing-masing kelompok diambil darahnya sebelum dan saat dipotong untuk diperiksa kadar kortisol dengan enzyme-linked immunosorbent assay (ELISA). Rata-rata kortisol sapi betina sebelum vs setelah restraint pada K1 dan K2 masing-masing adalah 55,64±52,13 vs 21,23±20,63 ng/ml dan 127,48±150,19 vs 106,28+75,39 ng/ml. Rata-rata kortisol sapi jantan sebelum vs setelah restraint pada K1 dan K2 masing-masing adalah 40,28±38,12 vs 24,21±17,21 ng/ml dan 49,51±38,67 vs 69,62±63,98 ng/ml. Rata-rata kadar kortisol tanpa memperhatikan jenis kelamin pada K1 dan K2 masing-masing adalah 79,50±88,50 vs 95,80±69,69 ng/ml (P>0,05). Disimpulkan bahwa tidak terdapat perbedaan kadar kortisol sebagai indikator stres pada sapi-sapi yang dipotong di RPH Yogyakarta yang mengalami restraint dengan restraining box tipe Mark 1 dan restraint metode konvensional.
PROFIL SARAF NITRERGIK SEKUM AYAM PEDAGING YANG DIINFEKSI EIMERIA TENELLA (THE PROFILE OF NEORAL NITRERGIC IN THE COECUM OF BROILER INFECTED WITH EMERIA TENELLA) Hana, Amelia; Astuti, Pudji; Fibrianto, Yuda Heru; ., Sarmin; Airin, Claude Mona
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (137.728 KB)

Abstract

Neurotransmitter found in the enteric nervous system that plays an important role in a variety ofenteropathies, including inflammatory bowel disease. Alteration of nitrergic neurons has been reported to bedependent on the manner by which inflammation is caused. This study was performed to determine theprofile of neural nitrergic with Nicotinamide Adenine Dinucleotide Phosphate-diaphorase (NADPH-d)staining in the cecum of broiler infected by Eimeria tenella (E. tenella). Thirty six health free of coccidiosisbroiler one-day old chickens (DOC) used in this study. All of broiler adapted for 7 days with food and drinkgiven ad libitum then divided into three groups, each group consisted of 12 broilers. The 1st group wascontrol only given orally 1.0 ml of distilled water/ heads , while 2nd group was infected with a single dose of5x103 oocysts/ head orally, and 3rd group was infected with a single dose of 2x104 oocysts/ head orally. Onday 7 post infection, all of chickens were fasted for 12 hours then were euthanized and cecum was taken.Lesion score of cecum was assessed. Furthermore, tissue of the coecum was prepared for hisochemicalusing NADPH-d staining to determine morphology, and morhometric of nitrergic neurons. The resultshown that cecum lesion score of group I is 0, group II is +2, group III is +3. Neuron density in the cecum ofgroup I is 2794.96+4.65 neuron/cm2, group II is 4930.36+63.73 neuron/cm2 and group III is 7892.31+44.97 neuron/cm2. Neuron density of group II and III increased signifcantly (p<001) than group I. Nitrergic neurondesity of group III increased significantly (p<0.05) than that of group II. It was concluded that the infectionof E.tenella led to increase the number of neutrl mienteric nitrergic of the cecum. The higher lesion score ofcecum led to increase the number of mienteric nitrergic neuron.
THE EFFECTS OF SOME IONOPHORE COCCIDIOSTATS ON THE INDEX OF DIGESTIVE TRACT WEIGHT OF BROILERS Hana, Amelia
Jurnal Sain Veteriner Vol 17, No 1 (1999)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8593

Abstract

Coccidiosis is one of the problems in poultry husbandry since it causes a lot of loss. Nowadays, several ionophore coccidiostats have been used to prevent coccidiosis. However, because of some complaints for ionphore coccidios­tats usage, especially Monensin which effect to the weight of digestive tract, therefore the use of coccidiostats should be taken with care. This investigation is intended to undestand the Increase of weight and some changes in diges­tive tract because of the use of 4 ionophore coccidiosis.This investigation used 52 Hubbard broiler of 1 days age. After 2 weeks of adaption 4 broiler were taken randomly as a control group (without coccidiostat). and the remaining 46 broilers were divided randomly into A group, that is. Monensin (Mo). Karacin (Na). Salinomicin (Sal), and Madura­micin (Mad)- The Mo group was divided futher into 3 sub­groups, those are Mo 70, Mo 110, and mo 130 that were fed containing Monensin 70, 110. and 130 mg/kg feed. The other groups were also divided similarly into 3 subgroups, those are, Na 50, Na 70. Na 90; Sal 40, Sal 60, Sal 80; and Mad 4, Mad 5, Mad 6. Number after the abbreviation of coccidiostats indicated the amount of mg/kg reed. The coccidiostats were used to the broilers since 14 until 45 days old. and finally all of the broilers were Killed 4 days later.Using variance analysis with split plot pattern showed that all of the coccidiostats did not effect to the weight growth of broilers (the weighting of broilers was carried out every week since 14 until 49 days old). The use of orthogonal polynomial contrast test indicated that the Monensin coccidiostat effected to the index (the related organ weight/the total weight x 100%) of praventriculus to Cloaca by an equation of VA = 26,9736 - 0,4602 X + G,0024X^P therefore the suggested dosage is 96,53 mg/kg feed. For the Maduramicin coccidiostat. besides it effected to the index of proventriculus-to cloaca by an equation of VR = -12.790 + 7,461 X - 0.696 X*. it also effected to the inffex of duode­num to cloaca by an equation of yc = -5,910+3,585 X - 0.315 X * therefore the suggested dosage is 4 mg/kg feed. The use of Naracin and Salinomicin did not influence to the Index of tractdigestive. All of the coccidiostats did not effect on the thickness of duodenum, jejunum, and ileum. covering diameter (by macroscopic measurement), also the indexes of epithelium/villi weight, mucosa layer thickness, and mus-culer layer thickness that measured by microscopic method-From this investigation can conlcuded that the use of Monensin in 96,53 mg/kg feed. Naracin In 50-90 mg/kg feed, Salinomicin in 40-80 mg/kg feed, and Maduramicin in 4 mg/kg feed will be safe, which means that they willnot increase the weight of tractusgastrointestine.
KADAR PROGESTERON NORMAL PADA SAPI PERAH IMPOR ESTRUS DAN SAPI POTONG LOKAL ESTRUS YANG DIPELIHARA DI DATARAN RENDAH Hana, Amelia
Jurnal Sain Veteriner Vol 22, No 2 (2004): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (961.303 KB) | DOI: 10.22146/jsv.480

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RESPONS PERISTALSIS DAN NEURON MIENTERIK NITRERGIK USUS HALUS KELINCI YANG DIINFEKSI EIMERIA MAGNA Hana, Amelia; Mangkoewidjojo, Soesanto; Isrina Oktavia Salasia2,, Siti; Liliek Kusindarta3, Dwi
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.154 KB)

Abstract

Penelitian ini bertujuan untuk mengetahui respon usus kelinci yang diinfeksi Eimeria magna denganmengamati frekuensi dan amplitudo kontraksi peristalsis, dan jumlah neuron mienterik nitrergik usushalus. Hewan percobaan yang digunakan adalah 60 ekor kelinci jantan lokal sehat umur 5 bulan dengankisaran berat badan 1,5-1,8 kg, dan bebas koksidiosis. Seluruh kelinci percobaan diadaptasikan dengankondisi lingkungan penelitian selama 7 hari dengan pakan pellet dan air minum ad libitum. Kelincidipelihara dalam kandang individual. Enam puluh ekor kelinci tersebut dibagi secara acak menjadi 3kelompok masing-masing 20 ekor. Kelompok I sebagai kontrol (K-0) diberi 1,0 ml akuades/ekor per oral,kelompok II (K-10) diinfeksi 10x106 ookista E. magna/ekor per oral dosis tunggal, dan kelompok III (K-20)diinfeksi 20x106 ookista E. magna /ekor per oral dosis tunggal. Pascainfeksi setiap hari 4 ekor per kelompokdianestesi dengan uretan (1,55 g/kg BB dalam larutan 25%, secara intraperitoneal), kemudian dibedah,diambil segmen usus halus (duodenum, jejunum, dan ileum). Segera setelah itu kelinci dibunuh dengancara dislokasi cervikal. Segmen usus halus diukur gerak peristalsis secara elektromiografik. Selanjutnyasampel tersebut dibuat preparat histokimia dengan teknik pewarnaan Nicotinamide Adenine DincleotidePhosphate-diaphorase (NADPH-d) untuk mengetahui jumlah neuron mienterik nitrergiknya. Data frekuensidan amplitudo kontraksi peristalsis, dan jumlah neuron mienterik nitrergik usus halus kelinci dianalisissecara statistika dengan sidik ragam dan uji-t (LSD). Hasil penelitian menunjukkan bahwa infeksi10x106 dan 20x106 ookista E. magna dapat menyebabkan timbulnya peningkatan frekuensi peristalsisusus halus (p<0,01), penurunan amplitudo kontraksi usus halus (P<0,01), dan penurunan jumlah neuronmienterik nitrergik usus halus (P<0,01) dibandingkan kelompok kontrol (K-0). Dari hasil disimpulkanbahwa infeksi ookista E. magna dapat menyebabkan peningkatan frekuensi peristalsis, penurunanamplitudo kontraksi, dan penurunan jumlah neuron mienterik nitrergik usus halus kelinci.
HIPERGLIKEMIA TIDAK MENYEBABKAN PERUBAHAN BOBOT BADAN DAN JUMLAH NEURON MIENTERIK NITRERGIK USUS HALUS TIKUS WISTAR (RATTUS NORVEGICUS) Hana, Amelia; Astuti, Pudji; Fibrianto, Yuda Heru; Sarmin, Sarmin; Airin, Claude Mona; Tauhid, Tauhid
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (11051.986 KB) | DOI: 10.22146/jsv.38409

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Digestive dysfunction is a common symptom of diabetes. Diabetes can lead to the loss of enteric neurons as well as nerve dysfunction and gastrointestinal complications. There has been no complete report of mienteric nitrergic of small intestines on hyperglycaemia condition, so the purpose of this study was to assess changes of nitrergic neural profile of small intestines on hyperglycemic Wistar strain rats (Rattus norvegicus). Twelve rats were adapted for 3 days, then they were randomly divided into 3 groups namely group I (K-1, as control), group II (K-2), and group III (K-3). Before treatment all rats were fasted for 12 hours, rats K-1 were induced with 1.0 ml of buffer citrate single peritoneal dose; rats K-2 were induced with 40 mg streptozotocin (STZ)/ kg body weight of single peritoneal dose; and rats K-3 were induced with 45 mg STZ /kg body weight of single peritoneal dose. On the 1st and 5th days after STZ induction, blood was collected through the coccigea vein to measure blood glucose levels and weigh the weight. On the day 5th the rats were uthanated with a high dose of ketamine, dissected and taken small intestines (duodenum, jejunum, and ileum) for staining with Nicotinamide Adenine Dinucleotide Phosphate-diaphorase (NADPH-d). Morphometric changes of nitrergic neurons of the small intestine segments were observed. The results showed that on the 5th day increased blood glucose levels of the K-2 and K-3 groups were significantly higher than K-1 (P <0.01). The weight of three groups did not show any significant improvement difference (P> 0.05). The number of neurons in the duodenum, jejunum and ileum of K-2 group increased higher than that of K-1 and K-3 groups. These results indicate that in hyperglycaemic states, the number of nitrergic neurons increases (K-2 group), and there is a significant decrease in the number of neurons (group K-3) compared with K-1 and K-2 groups (P <0.01). The results of this study concluded, that the STZ induction treatment causes hyperglycemia. Hyperglycemia does not affect to the body weight and the number of  nitrergic neurons of small intestines in Wistar strain rats (Rattus norvegicus).
THE HEART ACTIVITIES IN SOME ENVIRONMENTAL TEMPERATURE ON THE RATS (RATTUS NORVEGICUS) Hana, Amelia; Astuti, Pudji; Fibrianto, Yuda Heru; Sarmin, Sarmin; Airin, Claude Mona
Jurnal Sain Veteriner Vol 30, No 2 (2012): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5758.014 KB) | DOI: 10.22146/jsv.2609

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The aim of this research was to know the heart activity of rats (Rattus norvegicus) at some environmental temperature. Ten rats (Rattus norvegicus), male, 2 months old, the average weight of 200 gr purchased at experiment Animal Care Unit Gadjah Mada University, Yogyakarta. Allf rats were adapted in a cage with food and water ad libitum. Furthermore, each tail rat was measured heart activity is heart rate frequences (beats per minute), mean blood pressure (mmHg), systolic blood pressure (mmHg) and diastolic blood pressure (mmHg) at the environmental temperature of 30o C, 32o C, 34o C, 36o C, 37o C, 38o C, and 40o C  measured with a blood pressure cuff BP-98A at coccygea vein for 3 minutes at each temperature. The measurement was repeated 3 times. The data of the  heart activities obtained were analyzed by using one way analysis of variance. It was concluded that the environmental temperature of 30o C was optimal to the heart activities of the rats, including  heart rate frequences , blood pressure, systolic and diastolic blood pressures.Keywords:    heart rate frequences, blood pressure, systolic and diastolic, environment temperature, Rattus norvegicus
PENGARUH WARNA CAHAYA TERHADAP AKTIVITAS REPRODUKSI TIKUS PUTIH = THE EFFECTS OF COLOR OF LIGHT TO THE REPRODUCTION ACTIVITY OF WHITE RATS Hana, Amelia
Jurnal Sain Veteriner Vol 19, No 2 (2001): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.375

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Penelitian ini dilakukan dengan tujuan untuk mengetahui pengaruh pemberian warna cahaya terhadap aktivitas reproduksi tikus putih (Ranus norvegicus). Empat puluh ekor tikus putih jantan dan 20 ekor betina, semuanya umur 21 had., digunakan dalam penelitian ini. Tikus jantan dibagi menjadi 4 kelompok (IA, 11A, II1A, dan IVA) masing-masing- 10 ekor; dan tikus betina dibagi menjadi 4 kelompok (1B, IIB, IIIB, dan IVB) masing-masing 5 ekor. Tikus jantan dipelihara terpisah dengan tikus betina. Setelah adaptasi 6 hari, seluruh tikus ditimbang berat badan awalnya, dan kelompok IA, dan 113 diberi perlakuan cahaya putih (410 luks), I1A dan IIB cahaya biru (380 luks), IIIA dan 111B cahaya kuning (320 luks), dan IVA, dan IVB cahaya hijau (580 luks) setiap 12 jam sehari selama 30 hari. Setelah had ke-30 seluruh tikus ditimbang berat badannya dan setiap kelompok tikus jantan diambil 5 ekor dibunuh dan dinekropsi. Testis ditimbang dan dilakukan pengamatan morfologi, motilitas, persentase spermatozoa yang hidup, dan pH semen. Tikus betina yang estrus dikawinkan dengan tikus jantan, diamati lama kebuntingan dan jumlah anak yang dilahirkan. Berdacarkan hasil penelitian dapat disimpulkan bahwa lama pemberian cahaya yaitu 12 jam sehari selama 30 hari menyebabkan kualitas spermatozoa baik; sedangkan warna cahaya biru memperpendek masa kebuntingan dan meningkatkan jumlah anak sekelahiran dibandingkan 3 kelompok yang lain.
THE ADAPTATION OF SMALL INTESTINE NITREGIC MYENTERIC NEURONS ON RATS (RATTUS NORVEGICUS) TO HIGH FAT DIET Hana, Amelia; Sarmin, Sarmin; Airin, Claude Mona; Santosa, Christin Marganingsih; Astuti, Pudji
Buletin Peternakan Vol 43, No 2 (2019): BULETIN PETERNAKAN VOL. 43 (2) MAY 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (281.991 KB) | DOI: 10.21059/buletinpeternak.v43i2.38810

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High fat diet can result in the loss of nitrergic neurons in the myenteric plexus. The study aimed at finding out the effect of high fat diet on the adaptation of nitrergic nerve of rat intestine. It used 15 male rats (Rattus norvegicus) of a month of age with mean body weight of 53.73 gr. The rats were adapted for 7 days to individual cages with ad libitum feeding. After random adaptation, all of the rats were assigned to 3 groups of five rats, namely K-7, K-10, and K-13 groups. Feed and drinking water were given ad libitum. The treatment of the high fat diet lasted for 7 weeks. After the treatment, all of the rats were fasted for 12 hours and then killed. Subsequently, small intestine segments (duodenum, jejunum, and ileum) were taken. The intestinal segments were prepared by using NADPH-d histopathological technique to determine the morphometric changes of nitrergic myenteric neurons. During the treatment the rats were weighed every week and at the end of the study orbitalis vein blood measurement was carried out to see its glucose, cholesterol and cholecystokinin (CCK) plasma levels. The data of body weight, glucose, cholesterol, CCK levels, the total number of the nippergenic myenteric segments of the small intestine were statistically analyzed using Anova. The results of the study showed that the treatment of 7% to 13% fat diets for 7 weeks did not indicate any weight gain and any increase in cholescystokinin level, and any decrease in glucose level. However, it indicated significant increase in cholesterol level. The treatment of 10% and13% fat diets increased the total number of neurons in the jejunum and the ileum. Thus, it was concluded that the treatments of the high-fat diet of the rats (K-7, K-10, and K-13) for 7 weeks had significant effect on the adaptation of the neurons of the jejunum and the ileum.