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Journal : Journal of Tropical Horticulture

Potential Changes in Watermelon (Citrullus lannatus) Ploidy Treated By Colchicine Handayani, Rd. Selvy; Yusuf, Muhamad; Akmal, Ajmir
Journal of Tropical Horticulture Vol 1, No 1 (2018): October 2018
Publisher : Indonesian Society for Horticulture (Perhimpunan Hortikultura Indonesia Komisariat Aceh)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1406.163 KB) | DOI: 10.33089/jthort.v1i1.6

Abstract

The purpose of this study was to determine the effect of colchicine on changes in ploidy watermelon. The research was conducted in Agroecotechnology laboratory Universitas Malikussaleh, Microtechnique laboratory Agronomy and Horticulture Departement, Bogor Agricultural University, and Beuringen, Murah Mulia subdistrict, North Aceh. This research used Completely Randomized Design (CRD) two factors. The first factor was watermelon seed soaking time in colchicines 0,02% ie.0, 24,36, and 48 hours. The second factor was the concentration of the colchicine solution on the sprout growth point i.e. 0, 0,1, and 0,2 %. The results showed that plants were given colchicine became to shorter and fewer number of leaves than plants without any treatment. Colchicine could increase the size of the stomata, but it did not change the shape of stomata. Plants that were given colchicine had the potential to ploidy multiplication.
The Relationship between Transpiration and Calcium Fertilization on Mangosteen (Garcinia mangostana L.) Seedlings Akmal, Ajmir; Santosa, Edi; Poerwanto, Roedhy; Yunus, Ismadi; Handayani, Rd. Selvy
Journal of Tropical Horticulture Vol 1, No 1 (2018): October 2018
Publisher : Indonesian Society for Horticulture (Perhimpunan Hortikultura Indonesia Komisariat Aceh)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1559.113 KB) | DOI: 10.33089/jthort.v1i1.7

Abstract

The availability of nutrients in plants depends on the ability of plants to absorb nutrients from the soil. One of the processes absorbed by plants is by the presence of the transpiration process.  Good transpiration will provide enough nutrients for plants which increase the productivity and quality of mangosteen. One of the quality standards of mangosteen fruit for export quality is the free from yellow sap contamination. It can be possible that transpiration can improve the quality of mangosteen fruit by applying fertilization. In this study Ca fertilization was carried out on mangosteen seeds, where this study aims to investigate the effectiveness of Ca uptake by mangosteen plants with the level of water loss by transpiration. This research was conducted in November 2016-March 2017 at the Leuwikopo Experimental Garden, Bogor Agricultural University. Morphological observations were carried out in its Experimental Garden and the Postharvest Laboratory whileanalysis of calcium content of plant tissue was done at the Department of Agronomy and Horticulture, Bogor Agricultural University. Data obtained then were analyzed using ANOVA test. If the results were significant, Duncan Multiple Range Test (DMRT) was then tested at 0.05 probability level. The results revealed that the transpiration rate of mangosteen plants from several treatments showed a significant difference, the transpiration rate was higher, especially in fertilized plants. The size of the 12th leaf, plants treated with fertilizer have a larger size than the treatment without fertilization. Fertilizing and providing enough water can maintain the growth of the plants.
In-vitro Callus Induction of Durian (Durio zibethinus Murr.) Leaves Using Kinetin and 2,4-D (Dichlorophenoxyacetic acid) Handayani, Rd. Selvy; Yunus, Ismadi; Sayuti, M.; Irawan, Endri
Journal of Tropical Horticulture Vol 2, No 2 (2019): October 2019
Publisher : Indonesian Society for Horticulture (Perhimpunan Hortikultura Indonesia Komisariat Aceh)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1325.828 KB) | DOI: 10.33089/jthort.v2i2.23

Abstract

Durian (Durio zibethinus Murr.) is a tropical fruit grown in Southeast Asia and it has high nutritional and economic values and highly appreciated by consumers in Indonesia.  Unfortunately, there are several obstacles in planting durian in Indonesia, specifically in Aceh.  One of the abstacles is the scarcity of superior durian plants. Generally, plant conservations done conventionally through vegetative propagation.  However, this technique has disadvantage such as harming the initial plants.    Therefore, plant culture tissue has been used as a modern technique to develop durian plants. The objective of this research was to evaluate the effect of kinetin and 2,4 D on development of durian leaf explants through in-vitro bioassay. The research was conducted at Tissue Culture Laboratory, Faculty of Agriculture, Malikussaleh University from January to April 2019 using Completely Randomized Design (CRD) Factorial with 2 factors observed. The first factor was Kinetin (K): 0.0, 0.1 and 0.5 ppm. The second factor was auxin 2,4 D: 0.0, 0.5 and 1.0 ppm.  The results showed that the in-vitro application of kinetin affected the induction of leaf callus.  The application of 0.5 ppm of kinetin gave the best result compared to others.  The in-vitro application of 2,4 D 0.5 ppm also possessed the best result compared to others. There was no interaction beween in-vitro application of kinetin and 2,4 D on the growth of durian leaf callus at all parameters observed.
THE EFFECT OF DIFFERENT SEED CUTTING TREATMENTS AND CONCENTRATIONS OF BAP FOR THE SUCCESSFUL IN VITRO MICROGRAFTING OF MANGOSTEEN (GARCINIA MANGOSTANA L.) Agustina, Mira; Maisura, Maisura; Handayani, Rd. Selvy
Journal of Tropical Horticulture Vol 3, No 1 (2020): April 2020
Publisher : Indonesian Society for Horticulture (Perhimpunan Hortikultura Indonesia Komisariat Aceh)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1086.32 KB) | DOI: 10.33089/jthort.v3i1.37

Abstract

The efforts of the rooting of regenerants resulting from gamma-ray irradiation require plant tissue culture, which known as micrografting. This technique can help irradiated regenerants to develop a well root system, by combining non-rooting shoots with rooted in vitro cultured shoots of plant rootstock. The purpose of this study was to examine the effect of seed explants cutting and the application of different BAP concentrations for the successful micro-grafting of mangosteen in vitro. This experiment employed Complete Randomized Design (CRD) Factorial with two factors and ten replications. The first factor was the cutting treatments of mangosteen seeds explants for rootstock shoots, consisting of 2 types of seeds: uncut and cut seeds. The second factor was BAP concentrations: BAP 0 mg/l and BAP 2 mg/l. The results showed that the division of the seeds had an influence on the results of micro-grafting mangosteen in vitro. Micrografted mangosteen, which rootstock applied from undivided seeds, possessed faster growth, longer shoots, and produce more leaves compared to rootstock shoots from the divided seeds. BAP concentrations also contributed to the results. The application of BAP 2 mg/ demonstrated better effect on all variables observed. There were no interactions between seed divisions and BAP concentrations in all observed variables.
THE EFFECT OF BAP AND IBA ON IN VITRO ROOT CULTURES OF ACEHNESE POMELO (CITRUS MAXIMA (BURM.) MERR.) Handayani, Ira; Nazirah, Laila; Handayani, Rd. Selvy
Journal of Tropical Horticulture Vol 3, No 1 (2020): April 2020
Publisher : Indonesian Society for Horticulture (Perhimpunan Hortikultura Indonesia Komisariat Aceh)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1252.145 KB) | DOI: 10.33089/jthort.v3i1.43

Abstract

Acehnese pomelo is considered as a potential fruit to be developed for its economic and nutritional values. However, there is a main problem in developing this fruit. Pomelo sometimes is grown from seeds, but nowadays the fruits are often found to be having lesser seeds or even seedless. Besides, it is quite challenging to grow this local pomelo not only due to the scarce of the seeds, but it also cannot be conventionally germinated. Therefore, we need to develop this fruit through tissue culture. The purpose of this study was to investigate the effect of growth regulators on germination of pomelo seeds in vitro and its organ formation from in vitro root explants seed-originated of pomelo. The study was conducted at Plant Tissue Culture Laboratory, Faculty of Agriculture, Malikussaleh University. In October 2018 to April 2019. The research was divided into two separate experiments: 1) in vitro germination of pomelo seeds and 2) in vitro organ formation from root explants from of pomelo seeds. The first experiment using Complete Randomized Design (CRD) with 10 replications. The only factor observed was the use of BAP (0 and 2 mg/l). The second experiment was the examination of plant organ formation from root explants of pomelo seeds. This experiment had 2 factors observed. The first factor was different concentrations of BAP (0, 1, and 2 mg/l). The second factor was the different concentration of IBA (0, 1.25, and 2.5 mg/l). The results indicated that the application of BAP influenced the germination of pomelo seeds. The application of 2 mg/l BAP attributed to slower growth of shoots and roots, but it demonstrated better number of shoots, roots and leaves compared to application of 0 mg/l BAP. Pamelo root explants can form adventitious shoots on media eventhough without growth regulator (BAP 0 mg/l + IBA 0 mg/l), but optimization still needs to be done in order to obtain the best concentration to initiate shoot formation.