Tjodi Harlim
Faculty of Mathematic and Natural Science, Chemistry Department, Hasanuddin University, Makasar.

Published : 13 Documents
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A STIGMASTEROL GLYCOSIDE FROM THE ROOT WOOD OF MELOCHIA UMBELLATA (HOUTT) STAPF VAR. DEGRABRATA K. Ridhay, Ahmad; Noor, Alfian; Soekamto, Nunuk H.; Harlim, Tjodi; van Altena, Ian
Indonesian Journal of Chemistry Vol 12, No 1 (2012)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.21379

Abstract

A stigmasterol glycoside (stigmast-5,22-dien-3-O-?-D-glucopyranoside) has been isolated from the chloroform fraction of root wood of Melochia umbellata (Houtt) Stapf var. degrabrata K. The compound structure was determined on spectroscopic evidences including IR, 1D and 2D NMR and compared to previous data. The isolate was also active against Aspergillus niger.
A PRELIMINARY QUALITATIVE STUDY OF AN ALKALOID IN THE FRUIT OF TERONG BELANDA (CYPHOMANDRA BETACEA, CAV) Mandey, Fredryk; Dominggus, Mikael; Harlim, Tjodi
Indonesian Journal of Chemistry Vol 3, No 1 (2003)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.21898

Abstract

Preliminary studies on Terong Belanda (C. betacea ) have been carried out in this investigation with an aim to examine the presence of an alkaloid in the fruit of Terong Belanda. Several protocols had been done including maceration, extraction, evaporation, separation under column chromatography, crystallization and recrystallization, and testing with series of alkaloid reagent test. Finally one fraction, fraction D, obtaining in white sharp crystals, 96 mg, m.p 117.5 ºC gave positive results to an alkaloid especially from the group of atropine.
AKTIVITAS ANTIBAKTERI SENYAWA TURUNAN OLEANAN DARI KAYU AKAR PTEROSPERMUM SUBPELTATUM Salempa, Pince; Noor, Alfian; Hariani, Nunuk; Harlim, Tjodi
Jurnal Natur Indonesia Vol 13, No 1 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (47.458 KB) | DOI: 10.31258/jnat.13.1.59-62

Abstract

A triterpenoid derivative compound, oleane-12-en-2,3,23-triol-28-oat, from chloroform fraction of root wood ofPterospermum subpeltatum C. B. Rob (Sterculiaceae) was identified using IR, 1H dan 13C NMR data. The compoundhas shown toxicity against Artemia salina, LC50 of 46.06 ?g/mL, and antibacterial potential upon Shigella boydii andStaphylococcus aureus with inhibition zones of 8.3 and 9.8 mm respectively.
DISTRIBUSI LOGAM ESSENSIAL CU DALAM SPONGE (PORIFERA) DI KEPULAUAN SPERMONDE Melawaty, Lydia; Noor, Alfian; Harlim, Tjodi; Voogd, Nicole de
Marina Chimica Acta Vol 13, No 2 (2012): Volume 13 No. 2 (2012)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (192.906 KB) | DOI: 10.20956/mca.v13i2.1200

Abstract

A research on essential metal distribution of copper in Spermonde waters has been carried out. Sponge species of Melophlus sarassinorum, Callyspongia aerizusa, dan Clathria reinwardtii were identified and analyzed accordingly. Copper level were measured using Inductrively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) and the results showed that highest concentration was found in Clathria reinwartii  48.3 ppm. Copper distribution in sponge skeleton is higher than in its tissue and seemingly in terms of species the highest concentration is Clathria reinwartii taken from Barrang Lompo waters. In the meantime, Samalona waters contain highest concentration in its sedimen (3.96 ppm). Apparently Spermonde waters is beginning being interferred by metal pollution.
THE POTENTIAL OF CLATHRIA REINWARDTII AS BIOACCUMULATOR OF HEAVY METAL CU Melawaty, Lydia; Noor, Alfian; Harlim, Tjodi; Voogd, Nicole de
Marina Chimica Acta Vol 15, No 2 (2014): Metals in Marine Samples and Age Determination using Liquid Scintillation Count
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (715.686 KB) | DOI: 10.20956/mca.v15i2.1180

Abstract

Heavy metal Cu is an essential metal required by living organisms but a pollutant in water environment when existed in a level above the treshold level. The capability of sponge Clathria reinwardtii in accumulating heavy metals was analyzed according to modified Müller et al. (1998) method, and the sediment was analyzed according to Loring and Rantala in Rochyatun et al., (2006). Cu concentrations were measured by ICP-OES. The results indicate that Clathria reinwardtii was capable of accumulating the highest Cu level compared to other sponges. The highest Cu level analyzed in Clathria reinwardtii was 161.3 mg/kg.
THE POTENTIAL OF CLATHRIA REINWARDTII AS BIOACCUMULATOR OF HEAVY METAL CU Melawaty, Lydia; Noor, Alfian; Harlim, Tjodi; Voogd, Nicole de
Marina Chimica Acta Vol 15, No 2 (2014): Metals in Marine Samples and Age Determination using Liquid Scintillation Count
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (715.686 KB) | DOI: 10.20956/mca.v15i2.1179

Abstract

Heavy metal Cu is an essential metal required by living organisms but a pollutant in water environment when existed in a level above the treshold level. The capability of sponge Clathria reinwardtii in accumulating heavy metals was analyzed according to modified Müller et al. (1998) method, and the sediment was analyzed according to Loring and Rantala in Rochyatun et al., (2006). Cu concentrations were measured by ICP-OES. The results indicate that Clathria reinwardtii was capable of accumulating the highest Cu level compared to other sponges. The highest Cu level analyzed in Clathria reinwardtii was 161.3 mg/kg.
PRODUKSI SENYAWA BAKTERIOSIN SECARA FERMENTASI MENGGUNAKAN ISOLAT BAL Enterococcus faecium DU55 DARI DANGKE Razak, Abd. Rahman; patong, Abd. Rauf; Harlim, Tjodi; Djide, M. Natsir; Haslia, Haslia; Mahdalia, Mahdalia
Indonesia Chimica Acta Volume 2 No 2 - December 2009
Publisher : Indonesia Chimica Acta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Dangke is one of traditional food from Enrekang Province Sulawesi Selatan which is made from buffalo milk and enzimatically processed using papain from papaya’s gland secretion. Research on this local product as source of lactic acid bacteria (LAB) has been done. Counted 30 LAB was successfully isolated and 3 of them were potentially to be able to yield antimicrobial compound. Enterococcus faecium DU55 is one of LAB isolate available to be applied for producing bacteriocin compound through fermentation. Optimum condition of fermentation was specified by determining the highest antimicrobial activity generated by filtrate of fermentation result. Antimicrobial activity examination was carried out through diffusion agar method by measuring inhibition zone growth of pathogen bacterium Salmonella typhimurium FNCC 0050. Research results indicate that maximum bacteriocin compound production by BAL isolate E. faecium DU55 was obtained at condition of optimum fermentation at 30 °C during 42 hour using M1 medium with the same composition to medium MRS (Man Rogosa and Sharpe).Keyword: dangke, LAB isolate, bacteriocin, antimicrobial activity
Skrining Bioaktivitas Beberapa Bagian Jaringan Tumbuhan Paliasa(Melochia umbellata (Hout) Stapf var. Degrabrata K) Erwin, Erwin; Noor, Alfian; Soekamto, Nunuk Hariani; Harlim, Tjodi
Indonesia Chimica Acta Volume 2 No 1 - June 2009
Publisher : Indonesia Chimica Acta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Paliasa is one of tropical plants that used as an etnobotanical traditional drug by the people of South Sulawesi. Paliasa leaves is believed and used as a drug for hypertension, diabetes mellitus, cholesterol, and hepatitis diseases. Preliminary study was done by Brine shrimp lethality test with Artemia salina to a tissue part of one of type paliasa, Melocia umbellata (Houtt.) Stapf. var. degrabrata K. The result show that the wood of this plant is the most active with LC50 value of 1,80 ppm. Keywords : Paliasa, traditional drug, M. umbellata, bioactivity.
Uji Toksisitas Ekstrak methanol Beberapa Bagian Jaringan Tumbuhan Bayur (Pterospermum subpeltatum C.B. Rob) Salempa, Pince; Noor, Alfian; Hariani, Nunuk; Harlim, Tjodi
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 2 No 2 - December 2009
Publisher : Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v2i2.2462

Abstract

Bayur  plant    (Pterospermum  subpeltatum  C.B.  Rob)  has  not  been searched  for  their  secondary  metabolites.  However,  this  genes  was  used  as traditional   medicines  such as Pterospermum  javanicum   as cure for dysentry, toothache and Pterospermum  acerifolium   fer itchy drug. From toxicity test by BLST upon various tissues it was found that methanol extract, compared to other parts, has higher value 220 ppm.Keywords:  Pterospermum  subpeltatum  C.B. Rob, tocicity, traditional  medicine, Artemia salina
PRODUKSI SENYAWA BAKTERIOSIN SECARA FERMENTASI MENGGUNAKAN ISOLAT BAL Enterococcus faecium DU55 DARI DANGKE Razak, Abd. Rahman; patong, Abd. Rauf; Harlim, Tjodi; Djide, M. Natsir; Haslia, Haslia; Mahdalia, Mahdalia
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 2 No 2 - December 2009
Publisher : Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v2i2.977

Abstract

Dangke is one of traditional food from Enrekang Province Sulawesi Selatan which is made from buffalo milk and enzimatically processed using papain from papaya’s gland secretion. Research on this local product as source of lactic acid bacteria (LAB) has been done. Counted 30 LAB was successfully isolated and 3 of them were potentially to be able to yield antimicrobial compound. Enterococcus faecium DU55 is one of LAB isolate available to be applied for producing bacteriocin compound through fermentation. Optimum condition of fermentation was specified by determining the highest antimicrobial activity generated by filtrate of fermentation result. Antimicrobial activity examination was carried out through diffusion agar method by measuring inhibition zone growth of pathogen bacterium Salmonella typhimurium FNCC 0050. Research results indicate that maximum bacteriocin compound production by BAL isolate E. faecium DU55 was obtained at condition of optimum fermentation at 30 °C during 42 hour using M1 medium with the same composition to medium MRS (Man Rogosa and Sharpe).Keyword: dangke, LAB isolate, bacteriocin, antimicrobial activity