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HISTOPATOLOGI HATI MENCIT PASCA PEMBERIAN SUSPENSI KEPEL (STELECHOCARPUS BURAHOL) SECARA INTRAGASTRIK SELAMA 14 HARI THE HISTOPATHOLOGY OF MICE LIVER TREATED BY KEPEL (STELECHOCARPUS BURAHOL) SUSPENSION INTRAGASTRICALLY FOR 14 DAYS Harlina, Eva; S Darusman, Huda; Alvernita, Gita
FITOFARMAKA: Jurnal Ilmiah Farmasi Vol 2, No 1 (2012): FITOFARMAKA
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (416.895 KB) | DOI: 10.33751/jf.v2i1.162

Abstract

This aim of this study was to examine the effect of Kepel (Stelechocarpus burahol) to the mice hepatocytes. Thirty male mice of 4 week aged were divided into three groups;control group was treated by aquadest, Dose 1x group was treated by 2.6 mg/g BW/daykepel powder (0.5 ml kepel suspension/day), and Dose 5x group was treated by 13 mg/gBW/day kepel powder (1.0 ml kepel suspension/day). The treatment was intragastricallyfor 14 days. The mice were euthanized and necropsy followed by the liver collection forhistopathology assay. The histopathological examination of liver showed hydropicdegeneration, cell death and extramedullary hematopoietic observed on mice hepatocytes.The ANOVA analysis showed that kepel caused increase significantly (p<0.05) ofhydropic degeneration and decrease significantly (p<0.05) of cell death of micehepatocytes.Key words: Stelechocarpus burahol, hydropic degeneration, cell death, extramedullary hematopoietic
PAT-3 IN VITRO GROWTH INHIBITION ACTIVITIES OF NATURAL (NCAIFN) AND RECOMBINANT (RCAIFN) CANINE INTERFERONS ON THREE DIFFERENT TUMOR-DERIVED CELL LINES Priosoeryanto, Bambang Pontjo; Rostantinata, Rizki; Harlina, Eva; Noviyanti, Lina
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Recently, from many kinds of diseases one could be counted is a tumor disease. Tumor is a degenerative disease that involved many biological pathways within the host. A tumor or neoplasm can be defined as a disturbance of growth characterized by excessive, abnormal and uncontrolled proliferation of transformed or altered cell(s) at  one or more primary points within the host, and frequently at  one or more metastatic sites (Priosoeryanto, 1994).The treatment of tumor is mostly by medical surgery which usually combined with chemotherapeutic agent(s), unfortunately the using of chemotherapeutic agent can develop a seriously side effect to the treated-patients. Biological substances like interferon (IFN) known as anti-virus agent is also one of promising candidate for treating or preventing of tumor disorders.Feline and canine squamous cell carcinoma is one of tumor type that often found in cat and dog, and mostly develop to a very aggressive disorder. The using of in vitro cell culture is a way to avoid the use of live animals on the study of tumors especially the study of antitumor agent due to can mimic the in vivo condition.  The aim of the present study is to examine the growth inhibition activity of natural and recombinant canine interferon in order to find the suitable biological substances for combating tumor disorder especially in the field of veterinary medicine and also as an information for the development of tumor treatment in the human medical side.
Gambaran kristal urin pada hewan model nefrolitiasis hasil induksi etilen glikol Purwono, Rini Madyastuti; Wientarsih, Ietje; Widodo, Setyo; Purwaningsih, Erni H; Harlina, Eva
ARSHI Veterinary Letters Vol 3, No 1 (2019): ARSHI Veterinary Letters - Februari 2019
Publisher : Faculty of Veterinary Medicine, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (454.24 KB) | DOI: 10.29244/avl.3.1.19-20

Abstract

Tingginya tingkat keterulangan kasus urolitiasis baik pada hewan maupun manusia, menuntut penelitian dalam mencari solusi menurukan angka keterulangan. Penelitian urolitiasis membutuhkan hewan model urolitiasis yang sesuai dengan kondisi sebenarnya. Salah satu metode yang digunakan adalah menciptakan kondisi hiperoksaluria. Penggunaan induksi etilen glikol menjadi metode yang paling umum digunakan. Induksi ini menghasilkan kristal kalsium oksalat monohidrat. 
GAMBARAN DARAH DAN KALSIUM DOMBA LOKAL (Ovis aries) YANG DIIMPLANTASI BIFASIK KALSIUM FOSFAT 70/30 SCAFFOLD 3D BERPOROS Rahmiati, Dwi Utari; Gunanti, Gunanti; Harlina, Eva; Dahlan, Kiagus
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (121.823 KB) | DOI: 10.19087/jveteriner.2018.19.4.547

Abstract

The aim of this research is to study the biocompatibility biphasic calcium phosphate with alginate combination by evaluating blood and calcium concentration. This study used three male sheeps, age 1.5 year old with an average weight of 20 kg. Anaesthesia is using an intravenous anaesthetic combination of 10% ketamine HCl (2 mg / kg BW) and 10% xylaxine HCl (0.1 mg / kg BW). The implant material is grown on the right sheep tibia. As a control, the left sheep tibia was made of defects but not implanted. The incision orientation is the straight line of the tibial femoral joint at medial, 3.6 cm from caudal to cranial and perpendicular from that point 5.7 cm to the distal direction. The defect is made using a bone drill to make a hole according to the size of the implant (diameter 0.8 cm and 0.4 cm long). Blood parameters (erythrocytes, hemoglobin (Hb), hematocrit (Hct), total leukocytes and differentiation of leukocytes; neutrophils, eusinophils, basophils, lymphocytes and monocytes) and calcium evaluations were performed on days 0, 7, 30, 60 and 90 post implantation. Based on complete blood count no significant of blood parameters. Based on the calcium value, there are significant values on the 30th and the 90th. Blood calcium concentration were decreased from day 0 to 90.
Infusum Daun Alpukat Sebagai Inhibitor Kristalisasi Kalsium Oksalat pada Ginjal (THE AVOCADO LEAVES INFUSUM AS INHIBITOR ON RENAL CALCIUM OXALATE CRYSTALIZATION) Madyastuti, Rini; Widodo, Setyo; Wientarsih, Ietje; Harlina, Eva
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Urine crystal is a crystal nucleus which tend to form urine stone. The case of urine stone seems to beincreased every year. Crystallization could induce acute tubular necrosis which impact on renal dysfunction.The signs of this condition are high level of urea, creatinine and decrease glomerulus filtration rate. Theobjective of this research was to evaluate the effects of infusum Persea americana Mill as an inhibitorcrystallization which induced by ethylene glycol on white male rats. 20 male rats were divided into 4groups; K1 as negative group received only distilled water ad libitum, K2 as positive group receiveddistilled water containing ethylene glycol, K3 (dose 5%) and K4 (dose 10%) as treatment groups receivedwater containing ethylene glycol and avocado leaves infusion. Phytochemsitry screening of infusion avocadoleaves consisted of flavonoid, saponin, tanine and quinone. Result of analysis showed that the level ofureum and creatinine on K2 was higher than K3 and K4 group. The increased level could be inhibited byinfusion avocado leaves. The measurement of glomerular filtration rate in treatment groups wassignificantly different (p<0.05). Descriptive histopathology observation showed that renal lesio in grouptreatment (K3 and K4) were declined. Large crystal calcium oxalate on K2 group was observed by usingpolarized microscope, whereas small crystal calcium oxalate were seen in the infusion of avocado leavesgroups. These result showed the ability of infusion of avocado leaves as an inhibitor on the growth ofcrystallization calcium oxalate
HISTOPATOLOGI HATI MENCIT PASCA PEMBERIAN SUSPENSI KEPEL (Stelechocarpus burahol) SECARA INTRAGASTRIK SELAMA 14 HARI The Histopathology of Mice Liver Treated by Kepel (Stelechocarpus burahol) Suspension Intragastrically for 14 days Harlina, Eva; Sa’diah, Siti; S Darusman, Huda; Alvernita, Gita
FITOFARMAKA | Jurnal Ilmiah Farmasi Vol 2, No 1 (2012): FITOFARMAKA
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (416.895 KB)

Abstract

This aim of this study was to examine the effect of Kepel (Stelechocarpus burahol) to the mice hepatocytes. Thirty male mice of 4 week aged were divided into three groups; control group was treated by aquadest, Dose 1x group was treated by 2.6 mg/g BW/day kepel powder (0.5 ml kepel suspension/day), and Dose 5x group was treated by 13 mg/g BW/day kepel powder (1.0 ml kepel suspension/day). The treatment was intragastrically for 14 days. The mice were euthanized and necropsy followed by the liver collection for histopathology assay. The histopathological examination of liver showed hydropic degeneration, cell death and extramedullary hematopoietic observed on mice hepatocytes. The ANOVA analysis showed that kepel caused increase significantly (p<0.05) of hydropic degeneration and decrease significantly (p<0.05) of cell death of mice hepatocytes.Key words: Stelechocarpus burahol, hydropic degeneration, cell death, extramedullary hematopoietic 
Aktivitas Minyak Ikan dalam Menghambat Preneoplasia Kolon Mencit yang Diinduksi Azoksimetan dan Dextran Sodium Sulfate KUSMARDI, KUSMARDI; PRIOSOERYANTO, BAMBANG PONTJO; HARLINA, EVA; CORNAIN, SANTOSO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 12 No 2 (2014): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

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Abstract

Studi epidemiologi penggunaan suplemen minyak ikan dan pengaruhnya terhadap kanker&nbsp; kolon masih kontroversial. Penelitian ini dilakukan untuk melihat pengaruh minyak ikan dalam&nbsp; menghambat preneoplasia kolon mencit yang diinduksi azoksimetan (AOM) dan dextran sodium sulfate&nbsp; (DSS). Digunakan mencit Balb/C yang diinduksi dengan AOM 10 mg/kg BB yang dilanjutkan dengan&nbsp; pemberian minum DSS 1% selama seminggu. Pemberian minyak ikan dilakukan per oral dengan dosis&nbsp; 1,5, 3, dan 6 mg per mencit per hari. Pemeriksaan histopatologi terhadap jaringan kolon yang diwarnai&nbsp; hematoksilin-eosin dilakukan dengan menghitung jumlah fokus radang, hiperplasia, jumlah sel mitosis,&nbsp; dan skor displasia pada 10 lapang pandang. Penurunan jumlah fokus radang terjadi sejak bulan ke-2&nbsp; dimana kelompok kontrol dan dosis rendah berbeda dengan dosis sedang dan tinggi (p&lt;0,05). Sedangkan&nbsp; pada bulan ketiga dan keempat, penurunan jumlah fokus radang terjadi sejak dosis rendah, sedang dan&nbsp; tinggi (p&lt;0,01). Jumlah sel epitel kripta kolon mitosis mengalami penurunan mulai bulan kedua hingga&nbsp; keempat. Jumlah fokus sel epitel mukosa hiperplasia juga mengalami perbedaan pada bulan kedua.&nbsp; Pemberian minyak ikan juga menghambat terjadinya displasia yang terjadi sejak bulan keempat pada&nbsp; semua kelompok perlakuan, dan pada bulan ketiga pada kontrol
The Role of Fennel Infusion on Estrous Cycle and Follicles Development of White Rats MAHESHWARI, HERA; SATYANINGTIJAS, ARYANI SISMIN; HARLINA, EVA; CAHYANINGSIH, UMI; EFFENDI, MULYATI; MUSTOFA, MUHAMMAD ADIB; BEKALANI, YENI KEZIA
JURNAL ILMU KEFARMASIAN INDONESIA Vol 14 No 1 (2016): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

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Abstract

This study aims to describe the estrous cycle and follicles development in female rats given infusion of fennel fruit that known to have phytoestrogen compound. Twenty five of female rats were used in this research and were divided into 5 groups. Group I was negative control group (NC), given 1 mL/100g bw of distilled water, group II was positive control group (PC), given 0.0045 mg/100g bw of ethinyl estradiol, groups III, IV and V were treatment groups (TI, T2 and T3), given fennel infusion at 36.5; 73 and 146 mg/100g bw respectively. All treatments were conducted every morning for 20 days by oral route. Changes of vaginal epithelium were observed through vaginal swabs previously stained with Giemsa stain. Histopathological examination of ovarian swere examined to reveal follicles development. Results showed that fennel fruit infusion extended the duration of estrous and metestrous phases, while shortened the proestrous and diestrous phases. Eventhough the longest estrous phase was found in T3 group, there was no significant effect on the lengthening of estrous cycle. Moreover, infusion of fennel fruit had no effect on the development of ovarian follicles, except tended to increase the number of corpus luteum.
Hemogram dan Respon Jaringan Hernia Insisional Babi Pascaterapi Mesh Bedah Polipropilen dengan atau tanpa Asam Hyaluronat Vibowo, Heryudianto; Gunanti, .; Harlina, Eva
Acta VETERINARIA Indonesiana Vol. 7 No. 2 (2019): Juli 2019
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (864.716 KB) | DOI: 10.29244/avi.7.2.49-56

Abstract

Penggunaan mesh menjadi metode yang penting untuk terapi perbaikan hernia abdominalis karena dapat menurunkan angka kejadian hernia berulang dibandingkan dengan penggunaan metode penjahitan. Tujuan penelitian ini untuk mempelajari gambaran darah dan respon jaringan hernia insisional babi yang diterapi dengan mesh komposit dan polipropilen dengan atau tanpa asam hyaluronat secara histopatologi. Penelitian menggunakan babi sebanyak 11 ekor berumur 3-4 bulan yang dibagi menjadi 3 kelompok. Kelompok pertama mendapatkan mesh komposit (Physiomesh®), kelompok kedua mendapatkan mesh polipropilen (Ultrapro®) yang diberikan asam hyaluronat (Guardic®), dan kelompok ketiga mendapatkan mesh polipropilen (Ultrapro®). Penelitian dimulai dengan menginduksi hernia setelah itu dilakukan pemasangan mesh, permanenan jaringan dan mesh, dan pembuatan preparat histopatologi. Hasil penelitian menunjukan tidak adanya perbedaan yang nyata pada gambaran darah complete blood count antar kelompok. Hasil penelitian gambaran sel radang di jaringan juga tidak menunjukan perbedaan yang nyata kecuali hasil pengujian paired T-Test untuk makrofag pada kelompok mesh polipropilen. Sedangkan pada pengujian One-Way Annova tidak memberikan hasil yang berbeda nyata. Hasil penelitian ketebalan jaringan juga tidak menunjukan adanya perbedaan yang nyata antar kelompok. Dengan demikian pemberian asam hyaluronat tidak memberikan dampak negatif terhadap jaringan.
PAT-8 ANTIPROLIFERATION ACTIVITY OF KELADI TIKUS (TYPHONIUM FLAGELLIFORME) LEAVES ETHANOL EXTRACT ON MCA- B1 AND MCM-B2 TUMOR – DERIVED CELL LINES IN VITRO Rostantinata, Riski; Priosoeryanto, Bambang Pontjo; Harlina, Eva; Nurcholis, Waras; Noviyanti, Lina; Ridho, Rachmi
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (629.368 KB)

Abstract

INTRODUCTIONTumor  is  a  degenerative disease  as  the second agent cause of death in human. This disease is caused by disturbances of cell?s growth that show alteration and uncontrolled of cells proliferation. The uncontrolled of cells proliferation is also accompanied by penetration of that cells into others tissue and develop on it. Removed of tumor is usually done by operation or chemotherapy. Alternative treatment that can be used is herbal treatment. Typhonium flagelliforme known as ?Keladi Tikus? in Indonesia; is known has a chemopreventive effect (2). Other researchers also show flavonoid glucoside of T. flagelliforme (1) can induce the activity of apoptosis in colon cancer cells (5). The aim of this study is to examine the in vitro anti-proliferation activity of Typhonium flagelliforme leaves ethanol extract on MCA- B1and MCM-B2 cell line.