Articles

SELECTION OF COCONUT RESISTANCE TO THE NUTFALL DISEASE (PHYTOPHTHORAPALMIVORA BUTLER) THE OBJECTIVE OF THIS RESEARCH WAS TO SELECT THE COCONUT RESISTANCE TO NUTFALL PHYTOPHTHORA DISEASE. USING WOUNDING INOCULATION METHOD, DETACHEDHITS OF 11 COCONUT POPULATIONS AT PAKUWON COCONUT CROPPING PATTERN RESEARCH STATION WERE INOCULATED WITH PHYTOPHTHORA PALMIVORA CO5 ISOLATE. BASED ON THE DHEASE LESION SIZE AT SEVEN DAYS AFTER INOCULATION, GENJAH SALAK (GSK) COCONUTPOPULATION WAS MORE RESISTANT THAN THE Runtunuwu, Semuel D.; Sinaga, Meity S.; Hartana, Alex
Buletin Hama dan Penyakit Tumbuhan Vol. 11 No. 1 (1999): Buletin Hama dan Penyakit Tanaman
Publisher : Buletin Hama dan Penyakit Tumbuhan

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Selection of Coconut Resistance to the Nutfall Disease (Phytophthorapalmivora Butler) The objective of this research was to select the coconut resistance to nutfall phytophthora disease. Using wounding inoculation method, detachedhits of 11 coconut populations at Pakuwon Coconut Cropping Pattern Research Station were inoculated with Phytophthora palmivora Co5 isolate. Based on the dhease lesion size at seven days after inoculation, Genjah Salak (GSK) coconutpopulation was more resistant than the other coconutpopulations and showed individual resistance variation to the disease. From 238 GSK coconut trees selected, 226 (95%) trees were resistant and 12 (5%) trees were susceptible.
HUBUNAGN KEMAMPUAN PERGANTIAN INANG DENGAN PLASTISITAS GENETIKA PADA CENDAWAN BLAS PADI (PYRICULARIA GRISEA) Listiyowati, Sri; Widyastuti, Utut; Rahayu, Gayuh; Hartana, Alex; Jusuf, Muhammad
Jurnal Ilmu Pertanian Indonesia Vol. 14 No. 2 (2009): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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The Digitaria ciliaris, wild grass grown around rice field, was a host for Pyricularia grisea (Cooke) Sacc., the fungi caused blast disease of rice. This fungi have a specific mechanism to regenerate new genetic variation in its life cycle. The aim of this research is to study the relation between the ability of the fungi to infect different species of host with its genetic plasticity. It was used three SCAR molecular markers Cutl, Pwl 1 and Erg2. P. grisea isolates (Dc4J1) originated from D. ciliaris at Jasinga-Bogor were able to infect rice cultivars Kencana Bali and Cisokan. The original Dc4Jl, from D. ciliaris, and the Dc4Jl that were reisolated from the infected rice cultivars (reisolates-1) had the same ability to infect Kencana Bali and Cisokan. Molecular technique showed that there was a different molecular marker genotype between the original Dc4J1, from D. ciliaris, and the Dc4Jl reisolated from infected rice cultivars. The original Dc4J1 owned Cutl but did not Pwl2 in contrary the reisolates Dc4J1 from rice cultivars (reisolates-1) had Pwl2 but did not Cutl. The Erg2 presented in both the original and the reisolated Dc4Jl. These results indicated that there were a change of genotype of P. grisea at the same time with the change of host species. The Dc4Jl isolates originated from Kencana Bali and Cisokan (reisolates-2) that were infected by reisolate-1, had the same genotype with the reisolates-1.
TAKSONOMI MANGGA BUDIDAYA INDONESIA DALAM PRAKTIK Fitmawati, ,; Hartana, Alex; Purwoko, Bambang S.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 37 No. 2 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (49.283 KB) | DOI: 10.24831/jai.v37i2.1405

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The classification  of  cultivated plants should meet two approaches namely the botanical (classifying based on essential systematic plant characters) and the practical approach (clustering based on the analysis of commercial properties). This study analyzed taxonomy of mango cultivars grown in Indonesia based on morphology and agronomy characters. It obtained 84 recognizable cultivars.They are grouped into eigth main cultivar-groups (e.g. Berem, Madu, Gedong, Golek, Bapang, Arumanis, Kepodang, and Kebo) and eigthteen cultivar-groups. The 'Lalijiwo' cultivars synonym with 'Thaber', 'Tabar', 'Gurih' whereas 'Arummanis' synonim with 'Gadung'. Meanwhile, the cultivar 'Kates277' which is a member of Golek cultivar main group is homonim with the cultivar 'Kates' in Arummanis cultivars main group. Furthermore, the cultivar Nanas93 (one of Madu main group cultivar) that different with  the cultivar 'Nanas71' which is categorized as member of Bapang main cultivar group.   Key words: Taxonomy of cultivated plant, Indonesian mango, morphology, agronomy characters
PLANT SPECIES DIVERSITY OF PANGI BINANGGA NATURE RESERVE, CENTRAL SULAWESI Saleh, Muhammad F. R. M.; Hartana, Alex
Media Konservasi Vol. 22 No. 3 (2017): Media Konservasi Vol. 22 No. 3 Desember 2017
Publisher : Deparement of Forest Resources Conservation and Ecotourism - Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.322 KB) | DOI: 10.29244/medkon.22.3.286-292

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Pangi Binangga Nature Reserve is one of eight nature reserves in Central Sulawesi. In 1998, Pangi Binangga was designated as a conservation area, however, the diversty of the plant species not been recognized and reported. Pangi Binangga Nature Reserve is a lowland forest with vegetation and grassland forest, with primary and secondary forest vegetation. The objectives of this research are to provide informations of plant species in the Pangi Binangga Area. Exploration method was conducted throughout the region, and found 518 species. Rubiaceae, Euphorbiaceae, Moraceae, Compositae, Araceae, Urticaceae dominate in Pangi Binangga forest. Four species of them are endemic to Sulawesi, five species belong to endangered plants and 10 species of invasive. Keywords: biodiversity, endemic, invasive, Sulawesi
THE SEGREGATION PATTERN OF INSECT RESISTANCE GENES IN THE PROGENIES AND CROSSES OF TRANSGENIC ROJOLELE RICE Satoto, Satoto; Sulistyowati, Yuli; Hartana, Alex; -Loedin, Inez H. Slamet
Indonesian Journal of Agricultural Science Vol 9, No 2 (2008): October 2008
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Successful application of genetic transformation technique, especially in developing rice variety resistant to brown plant hopper and stem borer, will depend on transgene being expressed and the gene inherited in a stable and predictable manner. This study aimed to analyse transgene segregation pattern of the progenies and the crosses of transgenic rice cv. Rojolele harboring cry1Ab and gna genes. The third generation (T2) of fivetransgenic Rojolele events containing gna and/or cry1Ab were evaluated for two generations to identify the homozygous lines and to study their inheritance. The homozygous lines were selected based on the result of PCR technique. The segregation patterns of gna and cry1Ab were studied in eight F2 populations derived from Rojolele x transgenic Rojolele homozygous for cry1Ab and or gna and their reciprocal crosses. Data  resulted from PCR of F2 population were analysed using a Chi Square test.The study obtained six homozygous lines for gna, namely A22- 1-32, A22-1-37, C72-1-9, F11-1-48, K21-1-39, K21-1-48, and two homozygous lines for cry1Ab, namely K21-1-39 and K21- 1-48. Both cry1Ab and gna transgenes had been inherited through selfing and crossing with their wild type as indicated from the F1 containing gna and cry1Ab as many as 48.4% and 47.4%, respectively. In six of the eight crosses, gna was inherited in a 3:1 ratio consistent with Mendelian inheritance of a single dominant locus, while in the remaining two crosses, gna was segregated in a 1:1 ratio. The presence of cry1Ab in F2 populations also showed a 3:1 segregation ratio in all crosses. In the F2 population derived from F1 plant containing cry1Ab and gna, both transgenes segregated in a 9:3:3:1 dihybrid segregation ratio. This study will add to the diversity of geneticsources for insect resistance and allow further use of these transgenic lines for pyramiding resistance to brown plant hopper and stem borer or  separately in rice breeding programs whenever the efficacy tests and biosafety requirements have been completed.
ONTOGENETIC ALLOMETRY OF BODY HEIGHT AND BODY MASS OF GIRL IN BADUY, INDONESIA Rohmatullayaly, Eneng Nunuz; Hartana, Alex; Hamada, Yuzuru; Suryobroto, Bambang
HAYATI Journal of Biosciences Vol. 25 No. 3 (2018): July 2018
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (390.178 KB) | DOI: 10.4308/hjb.25.3.138

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Several small-scale populations exhibited phenotypic plasticity whereby growth spurt of body height occurred much earlier than age at menarche and this was not followed by same early spurt of body weight. This leads to question whether growth trajectory of stature follow the same growth trajectory of body mass and whether the trajectory itself is associated to sexual maturity. We evaluated developmental plasticity observed in Baduy girl, a traditional population in Indonesia, in facing strenuous environmental and biocultural conditions. We measured stature and body mass cross-sectionally. We determined age at menarche as population average of age of girls that had already got their first menstruation. Growths of body fat and weight followed a same mode and timing and their spurts pivoted on the age at menarche. In contrast, growth spurt of body height occurred four years earlier than menarche and velocity curve of body linearity progressed in opposite direction to that of body ponderality. The prevailing poor nutrition and high physical activity elicited principle of ontogenetic allometry to synchronize the acceleration and deceleration of growths in body linearity and ponderality whereby growth in body height functions to reach the body size target and to provide skeletal framework for development of body mass. The biocultural conditions lead to slow bodily growth rate with low spurt resulting in the characteristics of Baduy girl that was small in size and late in both sexual maturity and full-grown ages.
KARAKTER ROOT RE-GROWTH SEBAGAI PARAMETER TOLERANSI ALUMINIUM PADA TANAMAN PADI Roslim, Dewi Indriyani; Miftahudin, Miftahudin; Suharsono, Utut; Aswidinnoor, Hajrial; Hartana, Alex
Jurnal Natur Indonesia Vol 13, No 1 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.048 KB) | DOI: 10.31258/jnat.13.1.82-88

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Aluminum (Al) is one of the major limited factors in crop production on acid soils. Aluminum tolerant plants can beselected from plant breeding program by one of the physiological parameters representing Al tolerance character,such as root re-growth capability during recovery from the Al-stress. In this study we determined the concentrationand time exposure of Al stress that was able to differentiate the response of three local upland rice varieties(Grogol, Hawarabunar and Krowal) and an Al-sensitive rice variety (IR64) to Al-stress, and evaluated the effectivenessof root re-growth (RRG) characters as an Al tolerance parameter in rice. The study consisted of three experiments,which were 1) nutrient culture experiment with different Al concentration treatments in growth chamber, 2) potexperiment in greenhouse using Jasinga yellow red podzolic acid soil containing 26,66 me/100 g Al and pH 4,6 asplanting media, and 3) phenotyping of F2 population using RRG character. The results showed that Al treatment at15 ppm for 72 h was able to distinctly differentiate between Al-tolerant (Grogol and Hawarabunar) and Al-sensitivevarieties (Krowal and IR64). Planting of the rice varieties on acid soils showed similar result as that of the nutrientculture. Phenotyping of F2 population using RRG character indicated the existence of RRG value variation. Thesevariations demonstrated that RRG character can be used as an Al tolerance parameter in rice and therefore can beeffectively applied to screen rice F2 population that segregate to Al tolerance character.
GROWTH PATTERN OF BODY SIZE IN BADUY PEOPLE Rohmatullayaly, Eneng Nunuz; Hartana, Alex; Hamada, Yuzuru; Suryobroto, Bambang
HAYATI Journal of Biosciences Vol. 24 No. 2 (2017): April 2017
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1081.707 KB) | DOI: 10.4308/hjb.24.2.57

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Understanding body size growth pattern is one of the best ways to find out biological variation in phenotypic plasticity, health and nutritional statuses, and quality of life. Optimum environment and better nutrition are associated with rapid growth, tall stature, and early puberty. However, poor living condition impacts every stage of development, which results in variation in growth rates, growth periods, and body sizes across populations. Baduy is one of traditional populations who inhabit mountainous area in Kanekes Village, Lebak Regency, Banten Province, Indonesia. The traditional obligatory duties and taboos resulted in genetic, geographic, and cultural isolations. This leads to question whether the biocultural condition affects the growth pattern of their body size. We measured the body height, weight, and mass index of 340 girls and 239 boys aged 4?30 years sampled from 39 of 61 hamlets using cross-sectional method. We found that Baduy people had prolonged growth resulting in small body size because of slow rate and low spurt. This might be a selection to save body maintenance costs in biocultural condition with poor nutrition and high physical activity.
PERTUMBUHAN TANAMAN JARAK PAGAR (JATROPHA CURCAS L.) PADA LAHAN PASCA TAMBANG TIMAH DI BANGKA YANG DIBERI PUPUK ORGANIK Gedoan, Sukmarayu P; Hartana, Alex; Hamim, Hamim; Widyastuti, Utut; Sukarno, Nampiah
JURNAL ILMIAH SAINS Volume 11 Nomor 2, Oktober 2011
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jis.11.2.2011.205

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PERTUMBUHAN TANAMAN JARAK  PAGAR (Jatropha curcas L.) PADA LAHAN PASCA TAMBANG TIMAH DI BANGKA YANG DIBERI PUPUK ORGANIK Sukmarayu P. Gedoan1), Alex Hartana2), Hamim2), Utut Widyastuti2) dan Nampiah Sukarno2) 1)Program Studi Biologi, FMIPA Universitas Negeri Manado di Tondano; 2)Departemen Biologi, FMIPA, Institut Pertanian Bogor, Bogor 16680   ABSTRAK Kegiatan penambangan timah menyebabkan perubahan karakteristik fisika dan kimia tanah sehingga menjadi tidak sesuai untuk pertumbuhan tanaman.  Tujuan penelitian ini untuk mempelajari pertumbuhan 7 aksesi jarak pagar yang ditanam pada lahan pasca tambang timah yang diberi kompos dan kotoran sapi.  Penelitian ini dilakukan di TSS 133, Kelurahan Sinar Baru, Kabupaten Bangka, Provinsi Bangka Belitung.  Penelitian lapangan dilaksanakan pada Bulan Mei 2007 sampai dengan Bulan Desember  2008.  Percobaan faktorial ini dirancang dalam Rancangan Petak Terbagi dengan tiga ulangan.  Petak utama adalah 7 aksesi jarak pagar yang terdiri atas: aksesi Madiun, Ponorogo, Jember, Dompu, Lampung, Bengkulu,  dan Sukabumi, sedangkan  anak petak berupa tanah tanpa pemberian kompos dan pupuk kandang (kontrol), kompos trubus 4 kg/lubang ditambah 4 kg tanah bagian atas  dan  kotoran sapi 4 kg/lubang ditambah 4 kg tanah bagian atas. Hasil penelitian menunjukkan bahwa pertumbuhan awal yang paling baik untuk tanah bekas tambang timah adalah pada aksesi Jember yang ditunjukkan oleh diameter batang, berat kering tanaman, berat kering tajuk dan berat kering akar.  Sedangkan tinggi tanaman tertinggi diamati pada aksesi Madiun.  Penambahan kotoran sapi dapat meningkatkan produksi biji dan kandungan minyak.  Produksi biji tertinggi diperoleh pada aksesi Bengkulu dan kandungan minyak tertinggi diperoleh pada aksesi Dompu. Kata kunci: aksesi, Jatropha curcas, kompos, kotoran sapi   THE GROWTH OF CASTOR OIL PLANT (Jatropha curcas L.) ON THE POST-TIN-MINING LAND IN BANGKA PROVIDED WITH ORGANIC FERTILIZER ABSTRACT The activity of tin mining changes the physical and chemical characteritics of soil, so that the soil isnot suitable for plant growth.  The objective of this research was to evaluate the growth of some accession of Jatropha curcas that we planted on post-tin-mining land provided with compost and cow feces.   This research was conducted in TS 133, Sinar Baru Village, District of Bangka, Province of Bangka Belitung.  This field research was carried out in May 2007 to December 2008.  Factorial experiment was designed as split plot with three replications.  The main plot was 7 accessions, i.e. Madiun, Ponorogo, Jember, Dompu, Lampung, Bengkulu, and  Sukabumi.  The subplot waskonds of organic fertilizer, i.e. top soil without addition of compost and cow fecer (control), trubus compost  (4 kg/hole) added with top soil (4 kg/hole), and cow feces (4 kg/hole) added with topbsoil (4 kg/hole). The result showed that Jember accession had the best early growth based on the observation of stem diameter, plant dry weight, and root dry weight.  The largest plant height was observed in Madiun accession.  The higest seed production was observed in Bengkulu accession and Dompu accession had the higest oil content.Keywords: accession, Jatropha curcas, , compost, cow feces
INCREASING HERMAPHRODITE FLOWERS USING PLANT GROWTH REGULATORS IN ANDROMONOECIOUS JATROPHA CURCAS DASUMIATI, .; MIFTAHUDIN, .; TRIADIATI, .; HARTANA, ALEX; PRONOWO, DIBYO
HAYATI Journal of Biosciences Vol. 21 No. 3 (2014): September 2014
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1272.747 KB) | DOI: 10.4308/hjb.21.3.111

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Jatropha curcas (JC) is a crop with potential for use in biodiesel. Production of biodiesel requires plant seed as raw material, so the viability of JC for use in biodiesel will dependent greatly on the plant?s production of flowers. Generally, this plant is monoecious, meaning it has both male and female flowers. However, very rarely JC plants may be andromonoecious. Andromonoecious specimens of JC produce hermaphrodite and male flowers in the same plant. The number of hermaphrodite flowers per inflorescence is generally low compared to the number of male flowers. The aim of this study was to increase the proportion of hermaphrodite flowers by using plant growth regulators (PGRs) in andromonoecious JC. Our experiment was conducted in Randomized Block Design (RBD) with 9 treatments, namely kinetin, GA3, and IAA with concentrations of 0 ppm as a control, 50 and 100 ppm of each PGRs. The treatments were applied to stem cuttings from each plant and repeated 4 times. PGRs were applied by spraying the leaves within the buds of each plant. Applications took place weekly beginning when the plants entered flower initiating phase, until inflorescence produced. Observations were conducted during the treatment period (10 weeks). Results showed that plants treated with IAA, GA3, and kinetin at 50 and 100 ppm produced increased inflorescence per plant. The increases measured were 155.4 and 92.9% of (IAA), 120.4 and 151% (GA3), 96.6 and 51.7% (kinetin) respectively. In addition we found that application and GA3 at concentrations of 50 and 100 ppm, and kinetin at 50 ppm, increased the number of hermaphrodite flowers per inflorescence by 50%, and increased the number of hermaphrodite flowers per plant by 275.6 and 183.1% (IAA), 219.5 and 254.1% (GA3), 162.9 and 103.1% (kinetin) respectively. As would be expected, the number of fruit per plant increased in those specimens treated with IAA, GA3, and kinetin at 50 and 100 ppm. The increases measured were 301.7 and 167.4% (IAA), 211.7 and 257.0% (GA3), 162.5 and 101.4% (kinetin) respectively.