Tety Hartatik
Laboratorium Pemuliaan Ternak, Fakultas Peternakan, Universitas Gadjah Mada Jln. Fauna No. 5 Bulaksumur, Sleman, Yogyakarta 55281

Published : 16 Documents
Articles

Found 16 Documents
Search

DNA AMPLIFICATION OF MEAT TENDERNESS GENE OF BALI CATTLE Susilo, Agus; Hartatik, Tety; Artama, Wayan Tunas
Jurnal Ilmu dan Teknologi Hasil Ternak (JITEK) Vol 6, No 2 (2011)
Publisher : Jurnal Ilmu dan Teknologi Hasil Ternak (JITEK)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The aim of this study was to find out DNA band pattern as a result of amplification using DNA primer from meat tenderness gene of Bali cattle. Sample used in this study was DNA isolated from blood Bali cattle. Blood samples was collected in heparin tubes from jugular vein. Leucocyte cells were isolated using RBCs (Red Blood Cells) lysis buffer. To get DNA fragmen, marbling and meat tenderness gene were amplified by using DNA primer for for meat tenderness (forward: 5??CTACCGGGACGTCAACCT-3?; reverse: 5?-GGTTGTCGGGGTAGCTCA-3?). The size of DNA fragment were 210 bp. Key words: Bali cattle, meat tenderness gene
DNA AMPLIFICATION OF MEAT TENDERNESS GENE OF BALI CATTLE Susilo, Agus; Soeparno, Soeparno; Hartatik, Tety; Artama, Wayan Tunas
Jurnal Ilmu dan Teknologi Hasil Ternak (JITEK) Vol 7, No 1 (2012)
Publisher : Jurnal Ilmu dan Teknologi Hasil Ternak (JITEK)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The aim of this study was to know DNA band pattern as a result of amplification using DNA primer from meat tenderness gene of Bali cattle. Sample used in this study was DNA isolated from blood Bali cattle. Blood samples was collected in heparin tubes from jugular vein. Leucocyte cells were isolated using RBCs (Red Blood Cells) lysis buffer. To get DNA fragmen, marbling and meat tenderness gene were amplified by using DNA primer for for meat tenderness (forward: 5??CTACCGGGACGTCAACCT-3?; reverse: 5?-GGTTGTCGGGGTAGCTCA-3?). The size of DNA fragment were 210 bp, respectively. Key words: Bali cattle, meat tenderness gene
Accuracy of heart girth for predicting live weight of Aceh cattle Putra, Widya Pintaka Bayu; Hartatik, Tety; Sumadi, Sumadi; Saumar, Hendra
Jurnal Ilmu-Ilmu Peternakan Vol 24, No 3 (2014)
Publisher : Fakultas Peternakan Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (217.776 KB)

Abstract

Data on live weight (LW) at 550 days of age and body measurements such as withers height (WH), body length (BL) and heart girth (HG) of Aceh bull and heifer have been collected from 79 animals (39 males and 40 females) managed at the Indrapuri’s Breeding and Forages Centre (IBFC) of Aceh Cattle, Indrapuri District, Aceh Besar Regency, Aceh Province, Indonesia to estimate the LW from body measurements. The overall means (+ SD) for LW, WH, BL and HG of Aceh cattle over sexes were 129.37 + 30.84 kg, 96.59 + 7.11 cm, 93.10 + 11.77 cm and 118.15 + 11.08 cm, respectively. The study revealed that HG was the best predictor of LW and this variable alone contributed 70% (female), 86% (male) and 74% (all animals) of the variation in the LW of Aceh cattle. It was concluded that highest determination coefficient (R2) value of simple linear regression model was found on model LW (kg) = -172.47 + 2.59HG (cm), whereas this model were more effective and efficient for predicting the LW of male Aceh cattle. Key words: Aceh cattle, body measurements, live weight estimation, regression models.
Morphological diversity and genetic differentiation of PO cattle in smallholder farmers ., Hartati; ., Sumadi; ., Subandriyo; Hartatik, Tety
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 1 (2010)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.518 KB) | DOI: 10.14334/jitv.v15i1.680

Abstract

PO cattle is one of  the local cattle with high genetic diversity. The aim of this research was to study genetic diversity of PO cattle in smallholder farmer  based on morfology and molecular markers. This research was conducted at breeding center in East Java and Central Java include Tuban, Lamongan and Blora regency, since June until December 2008.  PO bull used were of 18 months until 24 months of age and cow of 24 months until 36 months of age or at first calving as much as 30 head each location. The variables measured were body weight, body length, withers height, hip height, shoulder point width, chest girth, chest depth, canon bone circumference, head length and head width. Data were analyzed using descriptive statistic, average analysis between subpopulations, discriminant and canonical analysis and mahalanobis distance was done by SAS DISCRIM procedure. Whole blood were collected to get the data of polymorphism DNA microsatellite using technology of PCR and electrophoresis using 6 microsatellite loci. Variable measured were number and size of  alel, alel frequency, heterozygosity,genetic distance and fixation index (F-stat). Data were analyzed using analysis of Microsate Toolkit, GENEPOPV4, MSA (Microsatellite Analyser) and Mega 4. The result showed that Blora and Tuban subpopulation have genetic diversity which is relative lower  compared to that of Lamongan. The genetic distance which was close presented by Tuban and Blora subpopulation while Lamongan and Tuban subpopulation show far genetic distance. The result of canonical analyses showed  high correlation on shoulder point width, head width, body length, withers height and hip height variable so this five variable can be used as distinguishing variables among subpopulation. The result of molecular genetics analysis using microsatellite showed that highest frequency of alel presented by HEL9 locus at Tuban population so this loci have the high polymorfism. The genetic differentiation among subpopulation showed by differentiation FST value among six loci  indicating the increasing of inbreeding in  the three subpopulations. Key words: Morphological Diversity, Genetic Differentiation, PO Cattle
The Application of Polymerase Chain Reaction – Restriction Fragment Polymorphisms (PCR-RFLP) to Determine Genetic Diversity of Madura Cattle in Sapudi Island Hartatik, Tety; Volkandari, Slamet Diah; ., Sumadi; ., Widodo
Indonesian Journal of Biotechnology Vol 18, No 1 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.889 KB)

Abstract

The aim of this study was to determine genetic diversity of Madura cattle using Polymerase Chain Reaction – Restriction Fragment Length Polymorphisms (PCR-RFLP) analysis of the cytochrome b (cytb) gene. Samples used for the experiments were blood of 43 cattle that consist of 15 cattle obtained from Madura Island, 23 cattle from Sapudi Island, and 5 Limousin-Madura (Limura) cattle. A fragment of 464 base pair of cytb gene was amplifi ed by forward primer L14735 and reverse primer H15149. The PCR product was digested with TaqIand HinfI restriction enzymes to identify genetic patterns. Data of PCR-RFLP showed two haplotypes, that were A and B, in cattle obtained from both Madura Island and Sapudi Island. The frequencies of haplotype A and B of cattle from Sapudi Island were 69.57% and 30.47%, respectively. More diverse frequencies were observed in cattle obtained from Madura Island, where haplotype A and B were 86.67% and 13.33%, respectively. In this experiment, Limura cattle had only haplotype A. As a conclusion, PCR-RFLP of the cytb gene had been able to determine a genetic diversity of Madura cattle.Key words: Genetic diversity, Madura cattle, haplotype.
Seleksi Awal Calon Pejantan Sapi Aceh Berdasarkan Berat Badan Putra, Widya Pintaka Bayu; Sumadi, Sumadi; Hartatik, Tety; Saumar, Hendra
Jurnal Sain Peternakan Indonesia Vol 10, No 1 (2015): Jurnal Sain Peternakan Indonesia
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penelitian ini bertujuan untuk  memilih calon pejantan sapi Aceh yang dipelihara di Balai Pembibitan Ternak Unggul – Hijauan Pakan Ternak (BPTU-HPT) Sapi Aceh Indrapuri berdasarkan berat badan ternak. Evaluasi yang dilakukan pada seleksi calon pejantan meliputi catatan berat lahir (BL), berat sapih (BS), berat setahunan (BY) dan berat dewasa (BD). Jumlah ternak yang digunakan untuk dievaluasi pada tahun 2014 adalah 34 ekor. Berdasarkan data catatan produksi diperoleh 10 calon pejantan sapi Aceh terbaik dengan kisaran nilai rasio berat dewasa (RBD) 1,03 sampai 1,65. Kesepuluh sapi Aceh ini akan direkomendasikan sebagai calon pejantan untuk progam uji zuriat pada tahap seleksi berikutnya.Kata kunci: sapi Aceh, catatan produksi, berat badan, rasio berat dewasa.
Morphological diversity and genetic differentiation of PO cattle in smallholder farmers ., Hartati; ., Sumadi; ., Subandriyo; Hartatik, Tety
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 1 (2010): MARCH 2010
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.518 KB) | DOI: 10.14334/jitv.v15i1.680

Abstract

PO cattle is one of  the local cattle with high genetic diversity. The aim of this research was to study genetic diversity of PO cattle in smallholder farmer  based on morfology and molecular markers. This research was conducted at breeding center in East Java and Central Java include Tuban, Lamongan and Blora regency, since June until December 2008.  PO bull used were of 18 months until 24 months of age and cow of 24 months until 36 months of age or at first calving as much as 30 head each location. The variables measured were body weight, body length, withers height, hip height, shoulder point width, chest girth, chest depth, canon bone circumference, head length and head width. Data were analyzed using descriptive statistic, average analysis between subpopulations, discriminant and canonical analysis and mahalanobis distance was done by SAS DISCRIM procedure. Whole blood were collected to get the data of polymorphism DNA microsatellite using technology of PCR and electrophoresis using 6 microsatellite loci. Variable measured were number and size of  alel, alel frequency, heterozygosity,genetic distance and fixation index (F-stat). Data were analyzed using analysis of Microsate Toolkit, GENEPOPV4, MSA (Microsatellite Analyser) and Mega 4. The result showed that Blora and Tuban subpopulation have genetic diversity which is relative lower  compared to that of Lamongan. The genetic distance which was close presented by Tuban and Blora subpopulation while Lamongan and Tuban subpopulation show far genetic distance. The result of canonical analyses showed  high correlation on shoulder point width, head width, body length, withers height and hip height variable so this five variable can be used as distinguishing variables among subpopulation. The result of molecular genetics analysis using microsatellite showed that highest frequency of alel presented by HEL9 locus at Tuban population so this loci have the high polymorfism. The genetic differentiation among subpopulation showed by differentiation FST value among six loci  indicating the increasing of inbreeding in  the three subpopulations. Key words: Morphological Diversity, Genetic Differentiation, PO Cattle
Pengaruh Proteksi Aldehid Untuk Meloloskan Poly Unsaturated Fatty Acid Pada Menir Kedelai dan Minyak Ikan Lemuru Secara In Vitro Riyanto, Joko; Baliarti, Endang; Yusiati, Lies Mira; Hartatik, Tety; Widayati, Diah Tri
Sains Peternakan: Jurnal Penelitian Ilmu Peternakan Vol 13, No 1 (2015): Sains Peternakan
Publisher : Universitas Sebelas Maret (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/sainspet.13.1.57-65

Abstract

This research was aims to determine the effect of formaldehyde using as a protective material in the soy groats and lemuru fish oil mixtured to escape of the protein and poly unsaturated fatty acid (PUFA) by in vitro. The rumen fluid was derived from donor of the Ongole crosbred fistulated as much as 3 heads. The experimental design used completely randomized design factorial 2x3 pattern consists of two factors:the first factor (soybean groats:lemuru fish oil in the ratio 2:1 and 4:1) and the second factor (the use of formaldehyde at levels of 0%, 2% and 4% base on the dry matter) and was repeated 5 times. The parameters are consist of the nutrient content, the substrates soybean groats and lemuru fish oil the rumen fluid fermented PUFA content by in vitro. Results of the study are levels of protein, fatty acids  oleic, linoleic and arachidonic much available on soybean groats:lemuru fish oil protected formaldehyde at levels of 2% and 4%. The difference between the ratio of soybean groats with lemuru fish oil had no significant (P≥0,05) but significantly different at different levels of formaldehyde (P≤0,05). The conclusion are formaldehyde content of 37% with a level of 2% can be used for protection the soybean groats and lemuru fish oil with a ratio of 4:1 and is able to provide protein as well PUFA. Keywords: soy groats, lemuru fish oil, in vitro, PUFA, formaldehyde protection
ESTIMASI NILAI PEMULIAAN DAN MOST PROBABLE PRODUCING ABILITY SIFAT PRODUKSI SAPI ACEH DI KECAMATAN INDRAPURI PROVINSI ACEH Putra, Widya Pintaka Bayu; (Sumadi), Sumadi; Hartatik, Tety
Buletin Peternakan Vol 38, No 1 (2014): BULETIN PETERNAKAN VOL. 38 (1) FEBRUARI 2014
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1258.797 KB) | DOI: 10.21059/buletinpeternak.v38i1.4594

Abstract

Penelitian ini bertujuan untuk membuat estimasi nilai pemuliaan (NP) dan most probable producing ability (MPPA) sifat produksi sapi Aceh di Kecamatan Indrapuri, Provinsi Aceh. Penelitian dilaksanakan mulai tanggal 7 Maret 2013 sampai dengan 7 April 2013 di Balai Pembibitan Ternak Unggul (BPTU)-Hijauan Pakan Ternak (HPT) Sapi Aceh Indrapuri. Materi penelitian ini terdiri dari catatan produksi dan populasi sapi Aceh tahun 2010 sampai 2012. Jumlah sapi yang digunakan untuk mengestimasi NP dan MPPA pada sifat produksi adalah 38 ekor. Estimasi nilai estimasi heritabilitas dengan metode korelasi saudara tiri sebapak (paternal half-shib correlation), sedangkan estimasi repitabilitas berdasarkan dua catatan individu menggunakan metode korelasi antar kelas (interclass correlation). Hasil penelitian menunjukkan bahwa nilai heritabilitas berat lahir, berat sapih, berat satu tahun, dan berat akhir masing-masing adalah 0,15+0,12, 0,48+0,58, 0,49+0,58, dan 0,56+0,69. Nilai repitabilitas yang dapat diestimasi berdasarkan data recording adalah berat lahir sebesar 0,33+0,22. Sapi Aceh pejantan nomor P.075 memiliki NPabsolut berat akhir tertinggi yaitu sebesar 14,99 kg. Nilai MPPAabsolut berat lahir tertinggi pada induk sapi Aceh nomor A.0668 yaitu sebesar 15,38 kg.(Kata kunci: Sapi Aceh, NP, MPPA, Heritabilitas, Repitabilitas, Sifat produksi)
SELEKSI PADA SAPI ACEH BERDASARKAN UJI PERFORMANS DI BPTU-HPT SAPI ACEH INDRAPURI Putra, Widya Pintaka Bayu; Sumadi, .; Hartatik, Tety; Saumar, Hendra
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 3, No 2 (2016): JITRO, Mei
Publisher : Jurnal Ilmu dan Teknologi Peternakan Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.851 KB)

Abstract

            Uji performans merupakan tahap seleksi awal untuk memilih calon pejantan dan induk yang terbaik. Penelitian ini bertujuan untuk mengevaluasi pertumbuhan pada sapi Aceh dewasa. Sebanyak 49 ekor sapi Aceh (23 jantan dan 26 betina) digunakan untuk uji performan selama 369 hari di BPTU-HPT Sapi Aceh Indrapuri. Rata-rata umur sapi sebelum diuji adalah sekitar 600 hari dengan berat awal terkoreksi (BAT) sebesar 110,08+16,25 kg (jantan) dan 108,25+21,08 kg (betina). Rata-rata berat akhir terkoreksi (BFT) sebesar 138,30+16,59 kg (jantan) dan 141,63+24,88 kg (betina). hari (betina). Rata-rata tinggi gumba (TG), panjang badan (PB) dan lingkar dada (LD) setelah pengujian pada sapi jantan masing-masing sebesar  99,07+4,64 cm,  94,41+6,21 cm dan 120,85+6,41 cm. Selanjutnya rata-rata nilai TG, PB dan LD pada sapi betina setelah pengujian sebesar 97,00+4,00 cm, 95,62+4,90 cm dan 124,25+9,38 cm. Nilai BFT tertinggi selama pengujian sebesar 166,71 kg (jantan) dan 214,19 kg (betina). Kata kunci: Sapi Aceh, uji performans, berat badan, ukuran tubuh