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MicroRNA-21 as a biomarker for ovarian cancer detection Kartika, Aprilia Indra; Chasanah, Siti Nur; Fitriawan, Akbar Satria; Tanjung, Dewi Sahfitri; Trirahmanto, Addin; Pradjatmo, Heru; Aryandono, Teguh; Haryana, Sofia Mubarika
Indonesian Journal of Biotechnology Vol 23, No 1 (2018)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (120.369 KB) | DOI: 10.22146/ijbiotech.35692

Abstract

Ovarian cancer is a lethal disease. One of the problems faced by patients with ovarian cancer is the lack of symptoms in its early stages, which results in it only being detected when it is at an advanced stage. Therefore, there is an urgent need for biomarkers that can predict ovarian cancer precisely. The purpose of this study was to determine the expression of microRNA-21 as a predictive biomarker candidate in both early- and advanced-stage ovarian cancer. This was a cross-sectional study using the blood plasma of 21 healthy control subjects and 37 blood plasma samples from patients with ovarian cancer. Blood plasmas were collected, from which the RNA was isolated. Based on the RNA, the cDNA was synthesized and run through qPCR, the results of which were analyzed using the Livak method. The results showed an upregulation of microRNA-21 in the advanced stage by 2.14 fold compared with the early stage, and 6.13 fold compared with the healthy controls (p < 0.05). The upregulation of microRNA-21 in early-stage ovarian cancer was 2.86 fold compared with the healthy control subjects (p < 0.05). In addition, there was an increase in the expression of microRNA-21 in ovarian cancer by 4.14 fold compared with the healthy controls (p < 0.05). Based on these results, it can be concluded that the expression of microRNA 21 upregulated with the severity of the disease.
Effects of soy isoflavone genistein on orthodontic tooth movement in guinea pigs Suparwitri, Sri; Pudyani, Pinandi Sri; Haryana, Sofia Mubarika; Agustina, Dewi
Dental Journal (Majalah Kedokteran Gigi) Vol 49, No 3 (2016): (September 2016)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (294.189 KB) | DOI: 10.20473/j.djmkg.v49.i3.p168-174

Abstract

Background: Osteoblast and osteoclast are the important factor in periodontal tissue remodeling for the orthodontic treatment success. Resorption process takes place in compression area by osteoclast and apposition in the tension area by osteoblast. In general hormone condition and age affect remodeling process. Estrogen has a high contribution in remodelling process and decreased in elderly individual such as menopausal women. Soybean contains isoflavone genistein which has similar structure and activity to estrogen. Many researchers indicate that isoflavone genistein not only has an inhibitor effect in osteoporosis but also has estrogenic and antiestrogenic effect as well. Purpose: The study aimed to investigate the effect of soybean isoflavone genistein administration on osteoblast and osteoclast cells number in orthodontic tooth movement of young and old guinea pigs. Method: The research was quasi-experimental study with post test only with control design. The experimental animals were 24 male guinea pigs that divided into: young guinea pigs (±4 months old) and old guinea pigs (±2.5 years old). Each group was divided into 4 subgroups for receiving the treatment namely; control, orthodontic treatment, genistein treatment and orthodontic+genistein treatment. All of the subjects were sacrificed at day 7 and the specimens were histologically analyzed using tartrate resistance acid phosphatase (TRAP) and hematoxylin eosin (HE) staining and observed using microscope that connected to obtilab and an image raster program. Result: U Mann-Whitney statistical analysis showed there were significant differences in osteoblast cell numbers; between orthodontic treatment and orthodontic+genistein treatment in the old guinea pigs (p=0.004); between orthodontic treatment in the young guinea pig and orthodontic+genistein treatment in the old guinea pig (p=0.016); between orthodontics treatment and orthodontic+genistein treatment in the young guinea pigs (p=0.025). U Mann-Whitney statistical analysis showed there were significant differences in osteoclast cell numbers: between the orthodontic treatment in the old guinea pig and orthodontics+genistein treatment in the young guinea pigs (p=0.007); between orthodontic treatment group in the young guinea pigs and orthodontics+genistein treatment in the old guinea pigs; between orthodontic treatment and orthodontic+ genistein treatment in the young guinea pigs (p=0.007). All groups administered by genistein the numbers of osteoblast in the surrounding of the tension sites increased, while in the surrounding of the compression sites had less osteoclasts; even, there were no osteoclasts found in some samples. Conclusion: Soybean isoflavone genistein administration on orthodontic tooth movement increased osteoblast numbers in the tension sides and decreased osteoclast numbers in the compression sides.
The Expression of hsa-miR-155-5p in Plasma Samples Of Breast Cancer Before And After Chemotherapy Fitria, Meutia Srikandi; Haryana, Sofia Mubarika; Anwar, Sumadi Lukman; Aryandono, Teguh; Tanjung, Dewi Sahfitri; Kartika, Aprilia Indra; Oktriani, Risky; Irianianiwati, .; Sari, Dwi Nur Indah
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 4 (2016): SUPPLEMENT
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (93.072 KB) | DOI: 10.19106/JMedScieSup0048042016018

Abstract

Breast cancer has emerged as the most common cancer-related mortality among women worldwide. Therefore, early cancer detection using biomarkers such as microRNA is needed. One of microRNAs that has an important role in breast cancer development is miR-155. Hsa-miR-155-5p is an oncomir that is commonly dysregulated in breast cancer. This study aims to determine the expression of hsa-miR-155-5p in breast cancer patient’s plasma before and after chemotherapy. We collected 64 samples from breast cancer patients admitted to Dr. Sardjito Hospital in Yogyakarta. RNA from plasma was extracted using RNA Isolation Kit miRCURY-Biofluid. cDNA synthesis was performed using cDNA Synthesis kit II and quantification of miR-155-5p using ExiLent SYBR Green master mix (Exiqon). qRT-PCR results were then analyzed with Livak's method and compared (before and after chemotherapy) with t-test. Expression of miR-155-5p in the breast cancer patients’ plasma after chemotherapy was significantly increased (10.59 times) when compared to before chemotherapy (p = 0.001). We concluded that there was upregulated expression of miR-155-5p after chemotherapy than before chemotherapy. There has not been a known, relevant pathway between hsa-miR-155-5p and chemotherapy regimens nor resistance to chemotherapy. Keywords: Breast cancer, plasma, hsa-miR-155-5p, oncomiR, chemotherapy.
Long Non-Coding RNA (lncRNA) and MicroRNA ( miRNA) in Cancer Management Haryana, Sofia Mubarika
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 4 (2016): SUPPLEMENT
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (109.794 KB) | DOI: 10.19106/JMedScieSup004804201630

Abstract

AbstractThe discovery of microRNA, a small non coding RNA, has shed light to the dark matters (98%) of the genome. This finding resulted in a Nobel Prize awarded to Fire and Mello in 2006. miRNA a small non coding RNA  which played  a very important role in regulating protein expression through  3”UTR  or other binding places to mRNA target. miRNA have been considered as negative regulators of protein coding gene expression that may impact in cell differentiation, proliferation,  survival and all fundamental cellular processes, also  implicated in carcinogenesis. miRNA can be grouped into tumor suppressor miRNA (miRSuppressor) and oncogenic miRNA (OncomiR). miRSuppressor regulates protein expression through targeting oncogenic mRNA, meanwhile OncomiR target mRNA Tumor Suppressor. Evidence indicates that deregulation in genetic and epigenetic may cause overexpression of oncomiR and loss of expression of Tumor Suppressor miR.  In addition to that, in recent years, evidences showed that cell-to-cell communication conducted via exosome, which is released from every cell in physiological and pathological conditions andconsidered as fingerprints of cell and its status. This is a paramount biomarker discovery in cancer. In subsequent years, a lot of research further performed for the development of new cancer therapy. Our team GenomiR present our preliminary data on several miRNA in cancers aimed to develop minimal invasive biomarkers in cancer. Recently, the long non coding (lnc) RNA, another class of non-coding RNA have also attracted interest from many scientists in the world. lncRNA have emerged as an essential regulator in almost all aspect of biology included carcinogenesis. lncRNA considered as emerging key player in non-coding world.nCRNA (miRNA and lncRNA) in the context of cancer management will be discussed in this presentation
Over- and down-expression mir-29c and mir-21 after chemotherapy and radio-therapy in nasopharyngeal carcinomas and the down-regulating proteins encoding eipstein barr virus and c-Myc. wardana, Tirta; Herawati, Cita; Oktriani, Risky; Lukman Anwar, Sumadi; Astuti, Indwiani; Aryandono, Teguh; Haryana, Sofia Mubarika
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 4 (2016): SUPPLEMENT
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.652 KB) | DOI: 10.19106/JMedScieSup004804201622

Abstract

Nasopharyngeal carcinoma (NPC) is the type of cancer related to multiple risk factors, including infection by Epstein Barr Virus (EBV). Standard treatment of NPC involves radiotherapy and chemotherapy in local and advanced tumors, while metastatic cases are treated with systemic chemotherapy. However, there is limited data on the causes of tumor recurrence, resistance, and progression. Moreover, the initial symptoms of NPC were often neglected until later enlarged, thus making it difficult to manage. MicroRNA (miRNA) is short molecule with 18-24 nucleotides and functions as protein-expression regulator protein in post-transcription. This study was aimed to determine miRNA expression and its relationship with the incidence of NPC. miR-21 and miR-29c were known to be involved in the development of NPC and resistance. A total of 51 plasma samples and 17 tissue samples were collected from Dharmais Hospital. The samples were taken from 17 untreated patients, 17 treated patients, and 17 healthy participants as control. We examined miRNA, protein of protein EBV (EBNA), and c-Myc expression using immunohistochemistry and quantitative polymerase chain reaction (qPCR). Our study revealed an increased expression of miR-21 and decreased expression of miR-29c in patients with NPC. There was also a correlation between the regulation of expression of miR-21 and c-Myc in the treated group of patients, and decreased expression in patients with complete response (CR) (4.13 ± 3.65: 2.74 ± 3.23; p <0.1). The parameters tend to increase in patients with partial response (PR) (3.00 ± 5, 86 compared to 8.77 ± 8.43; p <0.5), while no significant difference in expression of miR-29c in patients with CR and PR was detected. We concluded that miRNA might be detected in the plasma of NPC patients, and miR-21 might become a useful biomarker to determine therapeutic outcome in NPC patients.Keywords: nasopharyngeal cancer; miRNA; biomarker
Comparison of Bcl-xL protein expression in placental trophoblast cells between pregnancy complicated by severe preeclampsia and normotensive pregnancy Hadiati, Diah Rumekti; Palupi, Arsi; Hakimi, Mohammad; Haryana, Sofia Mubarika
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 1 (2018)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (373.436 KB) | DOI: 10.19106/JMedSci005001201804

Abstract

Preeclampsia is one of the main causes of maternal and perinatal mortality and morbidity.The pathogenesis of preeclampsia remains unclear until now. It is believed thatregulation of apoptosis in trophoblast cells plays an important role in the pathophysiologyof preeclampsia. Failure of spiral arteries remodeling will eventually lead to placentalhypoxia lead to excessive trophoblast apoptosis. The molecular mechanism of apoptosisis very complicated involving many signaling molecules included Bcl-2 proteins. The Bcl-2 protein group consists of proapoptosis proteins (Bax) and apoptosis inhibitor proteins(Bcl-2 and Bcl-xL). The aimed of this stuty was to compare the expression of Bcl-xLprotein in placental trophoblast cells of pregnancy complicated by severe preeclampsiawith that normotensive pregnancy. This study was an observational study with crosssectional design involving 43 pregnancy patients with severe preeclampsia and 38normotensive pregnancy who treated in Dr. Sardjito General Hospital, Yogyakarta fromOctober 2011 until March 2012. Placenta samples were obtained from all subjects forBcl-xL protein expression analysis using immunohistochemistry technique. Data wereanalyzed using independent t-test, chi-square test, and logistic regression. A p value<0.05 was considered significant. Significant difference in Bcl-xL protein expressionin trophoblast cells of pregnancy complicated by severe preeclampsia (1.29 ± 0.12)compared to that normotensive pregnancy (1.71 ± 0.14) was reported (p = 0.00). Inaddition, logistic regression test showed that diagnosis of severe preeclampsia had astatistically significant role in Bcl-xL protein expression (p= 0.000). In conclusion, theexpression of Bcl-xL protein is lower in pregnancy complicated by severe preeclampsiacompared to normotensive pregnancy.
Antioxidant Activity and Total Flavonoid of Carica papaya L. Leaves with Different Varieties, Maturity and Solvent Nisa, Fatma Zuhrotun; Astuti, Mary; Haryana, Sofia Mubarika; Murdiati, Agnes
agriTECH Vol 39, No 1 (2019)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (167.981 KB) | DOI: 10.22146/agritech.12813

Abstract

Carica papaya leaves are one of the vegetables consumed by Indonesian people, especially in Java Island. Carica papaya is easy to grow in Indonesia and has many variants, so, Carica Papaya leaves is a local potent to be developed for functional food and nutraceutical. The aim of this study was to investigate antioxidant activity and total flavonoids of Carica papaya leaves with different varieties, maturity and solvent. Carica papaya leaves (CPL) was firstly extracted by methanol to select two CPLs with high antioxidant capacity and total flavonoid. The two selected CPLs were further tested with different ages mainly young and mature leaves. One selected CPL was further tested with different extraction solvents. Antioxidant activity was determined by 2.2 diphenyl-1-picrylhydrazyl, DPPH and Ferric reducing antioxidant power, FRAP. This study used five varieties of Carica papaya leaves, namely Bangkok, California, Purple, Golden and Grendel. The result showed that Golden and Grendel varieties had a higher percentage of radical scavenging property than the others, which was 78.37% and 77.40% by the DPPH method. Grendel and Purple had a higher percentage of radical scavenging property, which was 45.82 and 34.32 mmol/mg. Grendel and Purple had a higher total flavonoid property, which was 50.33 and 46.02 µg/g. Mature leaves had a higher percentage of radical scavenging property than young leaves by DPPH and FRAP methods. Mature leaves had a higher total flavonoid property than young leaves in both Grendel and Purple. Grendel had a higher antioxidant activity and a higher total flavonoid property than Purple. Grendel with water extraction had a higher antioxidant activity by DPPH and FRAP methods. The total flavonoid of Grendel papaya leaves? extract with water extraction was lower than ethanol 70% and methanol.
Hubungan kadar IL-8 dan IL-10 yang berpengaruh terhadap progresifitas karsinoma nasofaring Savitri, Eka; Haryana, Sofia Mubarika
Oto Rhino Laryngologica Indonesiana Vol 44, No 1 (2014): Volume 44, No. 1 January - June 2014
Publisher : PERHATI-KL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.024 KB) | DOI: 10.32637/orli.v44i1.82

Abstract

Latar belakang: Karsinoma nasofaring (KNF) adalah keganasan epitelial yang banyak dijumpai pada populasi Cina dan Asia Tenggara termasuk Indonesia. Virus Epstein Barr (EBV) terbukti berasosiasi kuat dengan karsinoma nasofaring. Beberapa protein virus seperti EBER, LMP1, LMP2 ditemukan pada jaringan dan darah penderita kanker nasofaring. Selain itu terjadi peningkatan kadar antibodi terhadap protein virus IgA (VCA-p18+EBNA-1) pada mayoritas pasien. Peningkatan plasma EBV DNA merupakan marker yang penting adanya penyakit dan untuk monitoring progresifitas penyakit. Parameter lain serum/ plasma yaitu kadar Interleukin-8 (IL-8) dan interleukin-10 (IL-10) juga terlibat didalam progresifitas karsinoma nasofaring. Tujuan: penelitian ini untuk melihat hubungan kadar IL-8 dan IL-10 dengan stadium dari karsinoma nasofaring. Metode: Penelitian ini bersifat potong lintang (cross sectional study) pendekatan survei eksploratif dengan 39 pasien karsinoma nasofaring dan kontrol 29 orang sehat dalam penelitian ini. Nilai kadar plasma IL-8 dan IL-10 diperiksa dengan ELISA. Hasil: menunjukkan terdapat korelasi positif antara IL-8 dan IL-10 terhadap progresifitas KNF. Kesimpulan: IL-8 berhubungan dengan progresivitas karsinoma nasofaring. Rasio IL-8 dan IL-10 dapat digunakan menilai prognosis KNF. Bila hasil rasio IL-8:IL-10>1 menunjukkan tendensi buruk, oleh karena itu mungkin dapat diusulkan sebagai faktor prediktor karsinoma nasofaring. Kata kunci: Karsinoma nasofaring, IL-8, IL-10.0 ABSTRACTBackground: Nasopharyngeal cancer (NPC) is an epithelial malignancy, prevalent in Chinese populations and Southeast Asia including Indonesia. Epstein Barr Virus (EBV) has been assosiation with nasopharyngeal cancer. Viral gene products namely EBER. LMP1, LMP2 and EBNA?S have been found in nasopharyngeal cancer tissues. In addition increase of IgA (VCA-p18+EBNA-1) in the majority of patients. most often found in nasopharyngeal cancer tissues with an increase in IgA antibody titer of viral proteins (VCA-p18+EBNA -1) in the majority of patients. The increase of plasma of EBV DNA load is an important marker of disease and for monitoring its progression. Other parameters of serum/plasma is level of Interleukin-8 (IL-8) and interleukin-10 (IL-10) which also involved in nasopharyngealcancer progression. Purpose: to find out the relationship level of interleukin-8 (IL-8) and interleukin-10 (IL-10) in relation with the stadium of nasopharyngeal cancer. Method: A cross sectional study with exploratif survey was conducted 39 patients of NPC, controls 29 healthy subjects included in this study. The value of plasma levels of IL-8 and IL-10 examined by ELISA. Result: There was a positive correlation between IL-8 and IL-10 against the progression of NPC. Conclusion: IL-8 and is a marker of NPC and the progression of the disease. The ratio of IL-8 and IL-10 can be used to assess prognosis of NPC. Ratio of IL-8:IL-10 > 1 indicates a poor prognosis. Keywords: Nasopharyngeal, cancer, IL-8, IL-10