Articles

GENETIC VARIATION OF HUMPBACK GROUPER (Cromileptes altivelis) ON F1 AND F3 GENERATIONS Sembiring, Sari Budi Moria; Tridjoko, Tridjoko; Haryanti, Haryanti
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 5 No. 1 (2013): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (263.469 KB) | DOI: 10.29244/jitkt.v5i1.7755

Abstract

Hatchery and culture technology of Humpback grouper has been developed. However,  sometimes it is still found constraint in sedd production and caused instability of production due to failure by many factors. Breeding program become necessary to provide seed with high quality and good genetic variation. Quantitative characteristic selection is one of breeding program to improve productivity in grouper aquaculture. The aim of this research was to produce good phenotyphic and genotypic quality of Humpback grouper candidate broodstock. Selection of Humpback grouper F1 and F3 was done based on quantitative characteristic (body length and weight) and genotyphic characteristic by using ramdom amplified polymorphism DNA (RAPD) method.  The results of conventional selection were found candidate broodstock of Humpback grouper F1 and F3 with body weight range from 170-210 g and 160-170 g consecutively.  Heterozygosity values of Humpback grouper analized by RAPD of F1 and F3 were 0.7940 and 0.7749 consecutively and it was not significantly different. This value emphasis that population of this F3 Humpback grouper was still good to grow for broodstock. Keywords: genetic variation, humpback grouper, F1, F3.
PEMAHAMAN KOMPETENSI PARENTING TERHADAP PERKEMBANGAN SOSIAL ANAK (STUDI KASUS PADA KELOMPOK BERMAIN DI PAKEM, SLEMAN) Haryanti, Haryanti; Sumarno, Sumarno
JPPM (Jurnal Pendidikan dan Pemberdayaan Masyarakat) Vol 1, No 1 (2014): March 2014
Publisher : Departement of Nonformal Education, Graduate Scholl of Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.108 KB) | DOI: 10.21831/jppm.v1i1.2354

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Penelitian ini bertujuan untuk memahami kompetensi parenting orangtua, kendala dalam pengasuhan, dan strategi yang dilakukan. Penelitian ini merupakan penelitian kualitatif dengan subjek penelitian orangtua peserta didik pada lembaga kelompok bermain di Kecamatan Pakem. Teknik pengumpulan data dengan observasi, wawancara dan dokumentasi. Kompetensi parenting orangtua pada bentuk keluarga: (a) Nuclear family: orangtua cenderung mengikuti kemauan anak dan melarang anak bermain di luar rumah, karena orangtua berada di rumah. (b) Extended family: kecenderungan adanya perbedaan pola asuh orangtua dengan anggota keluarga lain. (c) Diverse family: kecenderungan orangtua memenuhi kebutuhan anak dengan bekerja sehari penuh, sehingga kurang waktu bersama anak. Kendala yang dihadapi orangtua di antaranya, orangtua yang bekerja dan mendelegasikan pengasuhan kepada kakek atau nenek, saudara yang lain, maupun kepada pengasuh. Strategi yang dilakukan orangtua adalah dengan melakukan pengaturan waktu dan melakukan pembatasan pergaulan anak dengan lingkungan, apabila dirasakan lingkungan tidak memberikan kontribusi positif terhadap perkembangan sosial anak.Kata kunci: kompetensi parenting, perkembangan sosial anak THE UNDERSTANDING OF THE PARENTING COMPETENCY ON THE SOCIAL DEVELOPMENT OF CHILDREN (CASE STUDY ON PLAYGROUPS IN PAKEM, SLEMAN)AbstractThis study aims to investigate parenting competency of parents, parents’ constraints in parenting, and strategies undertaken. This was a qualitative study with subjects were parents of students. The data were collected through observations, interviews, and documentation. Parents’ parenting competency, among others, the following: (a) Nuclear families: parents tend to follow the will of the child and tendency parents forbid children to play outside the house, because the parent is at home. (b) Extended familes: the difference in parenting from parents and other family members. (c) Diverse families: parents’ tendency to meet the needs of children with working of full day, so less time with children. Constraints faced by parent’s include the fact that they work so that they delegate it to grandfather or grandmother, other relatives, or babysitters. Parent’s strategies are that they manage time as effectively as possible and manage restriction of children’s socialization with the environment are made when parents think that the environment cannot give positive contributions to children’s social development.Keywords: parenting competency, children’s social development
Analisis Perubahan Penggunaan Lahan Di Kecamatan Pagelaran Kabupaten Peringsewu Tahun 2013-2018 Haryanti, Haryanti; Widodo, Sugeng; Miswar, Dedy
Jurnal Penelitian Geografi Vol 7, No 3 (2019): JURNAL PENELITIAN GEOGRAFI (JPG)
Publisher : Jurnal Penelitian Geografi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (922.018 KB)

Abstract

The aim of study was to analyze the change of land area, type of change in the use of land and the  factor causing the change  of  the  use  of  land  in Kecamatan Pagelaran Kabupaten Pringsewu in 2013-2018. The data obtained this research were (1) The area changed is 1.517.70 ha’s (20.94%) and the kind of change in the use of land were forest to moor, plantations to moor, farm to settlement, farm to moor, swamp to moor, moor to settlement and moor to farm. (2) The dominant factor that caused the change of the land used was the social economic factor which included of occupation and the increasing number of population.Penelitian ini bertujuan untuk menganalisis luas lahan yang berubah, jenis perubahan penggunaan lahan dan faktor yang menyebabkan perubahan penggunaan lahan di Kecamatan Pagelaran Kabupaten Pringsewu tahun 2013-2018. Hasil yang diperoleh dalam penelitian ini adalah (1) Luas lahan yang mengalami perubahan  yaitu seluas 1,517,70 ha (20,94%) dan jenis perubahan penggunaan lahan yang berubah adalah hutan ke tegalan, perkebunan ke tegalan, sawah ke pemukiman, sawah ke tubuh air ke tegalan, tegalan ke pemukiman dan tegalan ke sawah. (2) Faktor yang dominan menyebabkan perubahan penggunaan lahan adalah faktor sosial ekonomi yaitu pekerjaan dan bertambahnya jumlah penduduk.Kata  kunci:  faktor  penyebab  perubahan  penggunaan  lahan,  jenis perubahan penggunaan lahan, luas perubahan penggunaan lahan,
Biochemical Genetic Analysis Of Three Population Of Marble Grouper, Epinephelus polypekhadion Permana, Gusti Ngurah; Haryanti, Haryanti; Moria, Sari Budi; Giri, Nyoman Adiasmara; Sugama, Ketut
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 2 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.32

Abstract

Genetic variations of marble groupers, Epinephelus polypekhadion were evaluated to know genetic performance of fish to support fish seed production. Fifty fish samples from three locations i.e. West Nusa Tenggara, South Sulawesi and East Java were collected for analysis. Genetic analysis has been examined through allozyme electrophoresis by using 11 enzymes (ADH, GPI, SDH, IDH, LDH, ME, PGM, 6PGD, MPI, SP, EST). The result showed that 16 loci were detected, and three of them were polymorphism enzymes namely Isocitric dehydrogenase (IDH*), Glucose Phosphate Isomerase (GPI-1*) and Esterase (EST-2*). One locus (EST-2) was polymorphic in the East Java and West Nusa Tenggara populations and three loci (EST-2, GPI-1 and IDH) were polymorphic in the South Sulawesi population. The heterozygosity ranged from 0.007 to 0.034. Rogers genetic distance between population pairs were ranged from 0.005 to 0.012 (average = 0.009). Differences between genetic populations were significant (P<0.05). East Java and West Nusa Tenggara populations have same gene pool.
Polimorfisme Enzim Glucose-6-Phosphate Isomerase pada Tiga Populasi Tuna Sirip Kuning (Thunnus albacares) Permana, Gusti Ngurah; Hutapea, Jhon H.; Moria, Sari Budi; Haryanti, Haryanti
Jurnal Perikanan Universitas Gadjah Mada Vol 8, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.163

Abstract

Samples of yellowfin tuna (Thunnus albacares) were taken from three locations Bali, North Sulawesi and North Maluku. The glucose-6-phosphate isomerase (GPI) was analyzed from liver using allozyme electrophoresis method. Polymorphism of GPI enzyme was observed and four alleles (A, B ,C, D) were found in Bali population, three alleles (A,B,C) were found in North Maluku and North Sulawesi populations. Heterozygosity values, from Bali, North Maluku and North Sulawesi were 0.419; 0.417; 0.143 respectively. Genetic distance between North Sulawesi and North Maluku were 0.029, and with Bali population was 0.353. These results indicated that North Maluku and North Sulawesi population were not separate by geographic barrier, therefore genetic distance of both populations was closed. However, those populations were genetically separated to the Bali population might be due to major hydrological barrier.
GENETIC IDENTIFICATION FOR TUNA AND RAINBOW RUNNER CAPTURE IN NORTH BALI WATERS Permana, Gusti Ngurah; Hutapea, Jhon Harianto; Haryanti, Haryanti; Sembiring, Sari Budi Moria; Nakazawa, Akio
Indonesian Aquaculture Journal Vol 1, No 1 (2006): (June 2006)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (924.62 KB) | DOI: 10.15578/iaj.1.1.2006.29-34

Abstract

Gondol Research Institute for Mariculture identification of tuna and rainbow runner was an objective in this current study. Samples of five species were collected from territorial water of North Bali. The method used in this study was allozyme electrophoresis. The results showed that buffer of CAPM-6 (citric acid aminoprophylmorpholine) resulted in a sharp and clear banding pattern. The species could be differentiated in six diagnostic isozyme patterns Idh* (isocitrate dehydrogenase), 6Pgd* (6 phosphogluconate dehydrogenase), Gpi* (glucose phosphate isomerase), Mdh* (malate dehydrogenase), Est* (esterase), and Sp* (sarcoplasmic protein). All species were in Hardy-weinberg equilibrium. Heterozygosities of species were ranged from 0.00 to 0.099. Yellowfin tuna has the highest heterozigosity compared with the other species. Clustering samples according to pairs revealed that genetic distance of Bullet tuna (A. rochei) and Eastern little tuna (E. affinis) had small value (0.001). By contrast, the largest value was observed between yellowfin tuna, T. albacares and rainbow runner, E. bipunnulata (0.007). This value indicated that Bullet tuna (A. rochei) and Eastern little tuna (E. affinis) closed relation, while among yellowfin tuna, skipjack tuna, and rainbow runner, were separated phylogenically.
EFFECTIVITY OF INACTIVE GSDIV (GROUPER SLEEPY DISEASE IRIDOVIRUS) VACCINE IN GROUPER FISH (Cromileptes altivelis and Epinephelus fuscoguttatus) AGAINST GSDIV INFECTION Mahardika, Ketut; Mastuti, Indah; Haryanti, Haryanti
Indonesian Aquaculture Journal Vol 8, No 2 (2013): (December 2013)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1197.996 KB) | DOI: 10.15578/iaj.8.2.2013.143-151

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Grouper sleepy disease iridovirus (GSDIV) has been known as viral agent causing mass mortality and significant economic losses in Indonesian aquaculture industry. The aim of this research was to know the effectivity of formalin-inactivated GSDIV vaccine to prevent GSDIV infection in humpback grouper (Cromileptes altivelis) and tiger grouper (Epinephelus fuscoguttatus). The vaccine was derived from GF cells infected-GSDIV which activated using formalin. Used vaccine was contained titer virus of 102,80-105,80 TCID50/ mL. Result of vaccination test indicated that a vaccinated grouper fish with formalininactive GSDIV vaccine should decreased mortality rate of 5%–85.4% in humpback grouper and tiger grouper that infected with GSDIV. Histopathologically, infected fish from vaccinated and control groups showed formation of enlarged cells as well as necrotized cells especially in spleen and kidney tissues. On the other hand, surviving fish from both vaccinated and control groups did not contained formation of enlarged cells in spleen, kidney, liver, and stomach.
PHENOTYPIC AND GENOTYPIC PERFORMANCES OF BLACK TIGER SHRIMP, Penaeus monodon HAVING FAST GROWTH TRAITS Haryanti, Haryanti; Muzaki, Ahmad; Wardana, Ida Komang; Fachrudin, Fachrudin; Sembiring, Sari Budi Moria; Permana, I Gusti Ngurah
Indonesian Aquaculture Journal Vol 4, No 2 (2009): (December 2009)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.932 KB) | DOI: 10.15578/iaj.4.2.2009.101-108

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Shrimp breeding research focusing on fast growth traits is important to develop better phenotypic and genotypic characters of shrimp spawners. The aim of this research was to evaluate shrimp breeding technology to produce fry having fast growth traits. Selection was initiated from the breeding of wild shrimp spawners (F-0) based on full sib mating, probiotics, biosecurity, and viral diseases diagnosis. Viral disease diagnoses were performed using 7 viruses (TSV, WSSV, IHHNV, YHV, BP, MBV, and HPV) provided in the IQ-2000 kit. The result showed that 11 families of the first generation (F-1) shrimp were phenotypically varied (big, regular and small size). Number of fry from the first generation resulted from phenotypic selection that have fast growth trait ranged between 1.99% and 4.49% of the total fry population. Genotypic performance of the shrimp broodstock (F-0) and the first generation (F-1) showed different genetic variations. Heterozigosity values were 0.2872±0.0047 (female) and 0.5487±0.0103 (male) for shrimp broodstock (F-0), 0.704±0.0261 for the first generation (F-1) having fast growth trait, and 0.6224±0.023 for F-1 having slow growth. Gene markers of fast growth trait were indicated by the genes with molecular weights of 1,025; 1,280; and 1,325 basepairs and having different DNA sequences compared to that of slow growth shrimp.
KARAKTER GENETIK INDUK (F-0) DAN TURUNANNYA (F-1) PADA IKAN HIAS LAUT CLOWN (Amphiprion percula) MENGGUNAKAN MARKER RAPD (Random Amplified Polymorfism DNA) Sembiring, Sari Budi Moria; Setiawati, Ketut Maha; Haryanti, Haryanti; Wardana, Ida Komang
Jurnal Riset Akuakultur Vol 5, No 2 (2010): (Agustus 2010)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (137.351 KB) | DOI: 10.15578/jra.5.2.2010.183-190

Abstract

Studi analisis karakter genetik ikan hias laut clown menggunakan metode penanda DNA RAPD dilakukan dalam upaya membantu pengembangan perbenihan dan budidaya ikan hias laut clown di Indonesia. Tujuan penelitian ini adalah untuk mendeterminasi karakter genetik dengan menggunakan analisis individu dari populasi induk (F-0) dan turunannya (F-1) sehingga diperoleh tingkat penurunan keragaman genetik dan keterkaitannya dengan karakter morfologi. Sampel yang dianalisis terdiri atas 5 pasang induk ikan clown (10 sampel) dan masing-masing turunannya sebanyak 10 ekor (50 sampel) sehingga total 60 sampel. Nilai rata-rata keragaman genetik induk ikan clown dari semua lokus primer sebesar 0,253, sedangkan pada turunannya (F-1) adalah 0,157. Hal ini menggambarkan adanya pengaruh genetik terhadap perbedaan pola pemunculan band putih.Study genetic characteristic of clownfish, Amphiprion percula using RAPD DNA marker was conducted in order to support development of breeding and culture program of marine ornamental clownfish in Indonesia. The objective of this research was to determine of genetic characteristic of clown fish using individual analysis from F-0 population and its generations (F-1) to find specific marker which is related to its morphology. Total samples analyzed were 60, consist of 5 pairs of clownfish broodstock (10 samples) and 10 ind each generations (50 samples). Mean value of genetic diversity of clown fish broodstock from all primer loci was 0.253, while on its generation F-1 was 0.157. This result showed there was effect of genetic on the differences of white band pattern appearance.
PENENTUAN JENIS KELAMIN BERDASARKAN REAKSI ANTIBODI PADA IKAN TUNA Permana, Gusti Ngurah; Haryanti, Haryanti
Media Akuakultur Vol 2, No 2 (2007): (Desember 2007)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4153.25 KB) | DOI: 10.15578/ma.2.2.2007.77-79

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Pada budi daya ikan, identifikasi individu sangat penting untuk diketahui terutama dalam hal mengidentifikasi jenis kelaminnya. Bagaimana membedakan jantan atau betina ? Secara sepintas untuk membedakan ikan jantan atau betina memang mudah tetapi pada kenyataannya tidaklah demikian. Reaksi antibodi merupakan salah satu pendekatan yang dapat dipergunakan untuk penentuan jenis kelamin pada ikan yang telah menunjukkan aktivitas hormon dan pembentukan vitelogenin. Hasil dari kajian analisis dengan metode dot blot adalah ekspresi vitelogenin nampak jelas pada individu betina dan efek plasma terlihat transparan dibandingkan pada individu jantan tetapi metode ini sangat memerlukan pengalaman dan keahlian dalam akurasi pembacaan hasil.