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Journal : Jurnal Perikanan Universitas Gadjah Mada

Biochemical Genetic Analysis Of Three Population Of Marble Grouper, Epinephelus polypekhadion Permana, Gusti Ngurah; Haryanti, Haryanti; Moria, Sari Budi; Giri, Nyoman Adiasmara; Sugama, Ketut
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 2 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.32

Abstract

Genetic variations of marble groupers, Epinephelus polypekhadion were evaluated to know genetic performance of fish to support fish seed production. Fifty fish samples from three locations i.e. West Nusa Tenggara, South Sulawesi and East Java were collected for analysis. Genetic analysis has been examined through allozyme electrophoresis by using 11 enzymes (ADH, GPI, SDH, IDH, LDH, ME, PGM, 6PGD, MPI, SP, EST). The result showed that 16 loci were detected, and three of them were polymorphism enzymes namely Isocitric dehydrogenase (IDH*), Glucose Phosphate Isomerase (GPI-1*) and Esterase (EST-2*). One locus (EST-2) was polymorphic in the East Java and West Nusa Tenggara populations and three loci (EST-2, GPI-1 and IDH) were polymorphic in the South Sulawesi population. The heterozygosity ranged from 0.007 to 0.034. Rogers genetic distance between population pairs were ranged from 0.005 to 0.012 (average = 0.009). Differences between genetic populations were significant (P<0.05). East Java and West Nusa Tenggara populations have same gene pool.
Polimorfisme Enzim Glucose-6-Phosphate Isomerase pada Tiga Populasi Tuna Sirip Kuning (Thunnus albacares) Permana, Gusti Ngurah; Hutapea, Jhon H.; Moria, Sari Budi; Haryanti, Haryanti
Jurnal Perikanan Universitas Gadjah Mada Vol 8, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.163

Abstract

Samples of yellowfin tuna (Thunnus albacares) were taken from three locations Bali, North Sulawesi and North Maluku. The glucose-6-phosphate isomerase (GPI) was analyzed from liver using allozyme electrophoresis method. Polymorphism of GPI enzyme was observed and four alleles (A, B ,C, D) were found in Bali population, three alleles (A,B,C) were found in North Maluku and North Sulawesi populations. Heterozygosity values, from Bali, North Maluku and North Sulawesi were 0.419; 0.417; 0.143 respectively. Genetic distance between North Sulawesi and North Maluku were 0.029, and with Bali population was 0.353. These results indicated that North Maluku and North Sulawesi population were not separate by geographic barrier, therefore genetic distance of both populations was closed. However, those populations were genetically separated to the Bali population might be due to major hydrological barrier.
Pembenihan Selektif Kerapu Bebek, Cromileptes altivelis Turunan ke-2 (F-2) Tridjoko, Tridjoko; Haryanti, Haryanti; Muzaki, Ahmad; Sutarmat, Tatam
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (34.127 KB) | DOI: 10.22146/jfs.4

Abstract

The aim of this experiment was to know the gonad development of humpback grouper Cromileptes altivelis F-2 progeny. The F-2 progeny fishes were produced from selective breeding and composed by Lot 1, Lot 2 and Lot 3. The fishes were reared in the cylinder concrete tanks containing 75 m³, 10 m³ water and floating cages. Fishes were fed with commercial dry pellet in addition with vitamin mix, vitamin C and vitamin E. The result indicated that the mature gonad female of F-2 grouper broodstocks have been achieved. The female broodstocks with 590 g weight produced oocytes with diameter more than 400 µm. Genetic analysis showed that grouper Lot 2 was homogenous, did not find any polymorphism among individual fish, however Lot 1 and Lot 3 still showed high heterozygocity as 0.18 and 0.58, respectively.