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BACTERIA ASSOCIATED HOLOTHURIANS: THE KEY OF HABITAT PREFERENCE, DIET, AND FUNCTIO Hatmanti, Ariani; Purwati, Pradina
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 3 No. 1 (2011): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.821 KB) | DOI: 10.29244/jitkt.v3i1.7836

Abstract

An investigation of bacteria as holothurians’s feed carried out from May until June 2006 in Medane Bay and Kombal Bay, Lombok, Indonesia. The aims of this study are to know whether holothurians eat bacteria, to observe that bacteria are the key for holothurians’ habitat preference, and to understand the functions of bacteria in holothurians intestine. The results showed there are many kind of bacteria in the substrates and inside intestine of sea cucumber. There are no specificity in performance, species and number of bacteria which found in anterior or posterior of intestine, as well in their substrates. Therefore, the holothurians do not choose specific bacteria for their feed. Bacteria are the consequence of substrates ingested. Some of them can be microflora in holothurians intestine. Bacteria inside holothurians produce some enzymes such as protease, amylase and agarase. This function was proven by the increasing of Total Organic Matters (TOM) in their feces. This evidence supporting Wiedmeyer (1992) statement that sea cucumber adds organic matters in the sediment. So this also firm holothurians’ function in their habitat, as supplier for organic matters. The conclusion is that bacteria are eaten by holothurians by absorption, bacteria is not the key for microhabitat preference for holothurians, and inside the intestine of holothurians, digestion and degradation are the function of bacteria by producing their enzymes.Keywords: bacteria, holothurians, microhabitat preference, function
Potency of Actinomycetes from Deepsea Sediment of Makassar Strait for Producing Antimicrobial Substances Hatmanti, Ariani; Lisdiyanti, Puspita; Widada, Jaka; Wahyuono, Subagus
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 2 (2018): August 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i2.351

Abstract

A study on isolation of actinomycetes from sediments of Makassar Strait have been conducted with regard to research project called Widya Nusantara Exploration (EWIN) in May-June 2013 and November 2014. The objectives of this research were to screen antimicrobial activity of 36 actinomycetes from sediments of Makassar Strait, characterized the potential isolates, and determined the metabolites produced by the selected isolate. The antimicrobial screening was conducted using agar diffusion method, while characterization of the best five of actinomycetes were using APIZYM Kit, Scanning Electron Microscope, and FTIR. Five isolates retrieved from this research had ability to inhibit the growth of four microbial testing: Escherichia coli, Bacillus subtilis, Staphyllococcus aureus and Candida albicans. The highest capability was shown by the MACMK-43 isolate that had 16S rRNA gene sequence similarity of 97.85% to Streptomyces violacens. The result shows that, the active fraction contained of 4-amino-5-cyano-6-(4’methoxyphenyl)-1-methyl-2.3-dihydropyrrolo [2,3-B] pyridine, which is commercially used for bactericide and antihistamine.
ISOLASI DAN KARAKTERISASI BAKTERI PENGHASIL SENYAWA ANTIBAKTERI YANG BERASOSIASI DENGAN KARANG BATU DARI PERAIRAN BITUNG DAN SPONS DARI SELAT MAKASSAR SUNNY, FRANSISCA; KURNIATI, TRI HANDAYANI; HATMANTI, ARIANI
Bioma Vol 11 No 1 (2015): Bioma
Publisher : Biologi UNJ Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (369.545 KB) | DOI: 10.21009/Bioma11(1).5

Abstract

ABSTRACT Recently the needs of antibacterial compounds is increasing. This is due to the bacterial resistence to common antibacterial  compounds.  coral  and  sponge-ssociated  bacteria  are  potential  producer of antibacterial compounds. This research was aim to obtain coral and sponge-associated bacteria that could produce antibacterial compound. coral associated-bacteria was isolated from Bitung and   was isolated in Marine Agar by pour plate method. The antibacterial compounds were obtained by extraction using ethyl acetate and acetone. The antibacterial assay was performed by agar diffusion method using paper discs and was performed by testing with  Staphylococcus  aureus,  Bacillus  subtilis, Vibrio cholerae biotipe El Tor, and Escherichia coli. Total 37 isolate  was  isolated  from  corals and 25 isolate from sponge obtained from Selat Makassar. Based on the assay, only bacteria from sponge that showed antibacterial activity. Two sponge-associated bacteria, S.5-8 and  S.2-1 NRBC were found to inhibit S. aureus. From those isolates, isolate S.5-8 produced bigger clear zone (2,6 mm) than S.2-1 NRBC (1,5mm). S.5-8 could hydrolize gelatine whereas S.2-1 NRBC showed positive result on oxidase test and was able to fement xilose and arabinose to produce acid.   Key words: antibacterial activity, association, characterization, coral, isolation, sponge
Diversity of Culturable Actinomycetes from Deepsea Floor of Makassar Strait, Indonesia Hatmanti, Ariani; Lisdiyanti, Puspita; Widada, Jaka; Wahyuono, Subagus
Oseanologi dan Limnologi di Indonesia Vol 3, No 2 (2018)
Publisher : Oseanologi dan Limnologi di Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/oldi.v3i2.205

Abstract

With regard to collaboration research called Widya Nusantara Exploration (EWIN) in May-June 2013 and November 2014, a study on isolation of actinomycetes from sediments of Makassar Strait have been conducted. Actinomycetes is one of microbe which has an excellent track record in producing antimikrob and other active substances. But due to terrestrial actinomycetes has been widely explored, then recently researchers began focusing on wide variety of extreme environments, such as marine environment, to screening aktinomisetes in producing new secondary metabolites. A total of 36 strains of actinomycetes were isolated from 10 samples obtained from deepsea floor in Makassar Strait, Indonesia, Direct Dillution Method were best used to isolate the actinomycetes compare to Sodium Dodecyl Sulfida – Yeast Extract Method (SDS-YE Method) and Rehidration Centrifugation Method (RC Method). NBRC-802 media and Actinomycetes Isolation Agar(AIA)(Himedia)media were used as the isolation media. All the isolates were identified by morphological characteristic and by analysis of 16S rRNA gene sequence. Actinomycetes isolated from deepsea floor of Makassar Strait have been dominated by Micromonospora (58%), Verrucosispora (14%)Streptomyces (8%) and Luteipulveratus (5%), however genus Nocardiopsis, Micrococcus, Gordonia, Kytococcus, and Arthrobacter were not dominant (3%). Station 25 in 1.547 m depth was the most abundant of actinomycetes, 18 strains and dominated by the genus Micromonospora which is isolated using Direct Dillution Method and both NBRC 802 or AIA media.
HYDROCARBONOCLASTIC BACTERIA FROM JAKARTA BAY AND SERIBU ISLANDS Darmayati, Yeti; Harayama, Shigeaki; Yamazoe, Atsushi; Hatmanti, Ariani; Sulistiani, -; Nuchsin, Ruyitno; Kunarso, Djoko Hadi
Marine Research in Indonesia Vol 33, No 1 (2008)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9627.525 KB) | DOI: 10.14203/mri.v33i1.506

Abstract

Jakarta Bay has been known as one of the most polluted marine environment in Indonesia, with no exception by oil. Seribu Islands waters, located in the north of Jakarta Bay may have been impacted by this polluted condition.It’s sometimes also hit by oil spillage from pipe leakage. The purpose of this study is to isolate and identify hydrocarbonoclastic bacteria (oil and Polyaromatic Hydrocarbon degrading bacteria) from Jakarta Bay and Seribu Island waters. The bacteria were isolated from water and sediment/sand. Isolation was prepared by enriched samples in SWP medium with Arabian Light Crude Oil (ALCO). Screening for PAH degrading bacteria has been completed by using sublimation plate method in ONR7a medium and screening for oil degrading bacteria were conducted by using oil plated method with the same medium. Bacteria identifications were done based on l6sRNA gene. The results were analyzed using BLAST and showed that 131 potential hydrocarbonoclastic bacteria have been isolated from Jakarta Bay and Seribu Island waters. Most of them were oil degrading bacteria (41.98%) and the rest were PAH degrading bacteria. Oil pollution level may impact the number of strain of hydrocarbonoclastic bacteria isolated. Among the hydrocarbonoclastic bacteria isolated from Jakarta Bay and Seribu Islands, Alcanivorax, Marinobacter, Achromobacter and Bacillus were common hydrocarbonoclastic genera in Jakarta Bay and its surrounding waters. Alcanivorax spp. is important oil and PAH-degrader found not only in temperate waters, but in tropical waters as well.
HYDROCARBONOCLASTIC BACTERIA FROM JAKARTA BAY AND SERIBU ISLANDS Darmayati, Yeti; Harayama, Shigeaki; Yamazoe, Atsushi; Hatmanti, Ariani; Sulistiani, -; Nuchsin, Ruyitno; Kunarso, Djoko Hadi
Marine Research in Indonesia Vol 33 No 1 (2008)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9627.525 KB) | DOI: 10.14203/mri.v33i1.506

Abstract

Jakarta Bay has been known as one of the most polluted marine environment in Indonesia, with no exception by oil. Seribu Islands waters, located in the north of Jakarta Bay may have been impacted by this polluted condition.It?s sometimes also hit by oil spillage from pipe leakage. The purpose of this study is to isolate and identify hydrocarbonoclastic bacteria (oil and Polyaromatic Hydrocarbon degrading bacteria) from Jakarta Bay and Seribu Island waters. The bacteria were isolated from water and sediment/sand. Isolation was prepared by enriched samples in SWP medium with Arabian Light Crude Oil (ALCO). Screening for PAH degrading bacteria has been completed by using sublimation plate method in ONR7a medium and screening for oil degrading bacteria were conducted by using oil plated method with the same medium. Bacteria identifications were done based on l6sRNA gene. The results were analyzed using BLAST and showed that 131 potential hydrocarbonoclastic bacteria have been isolated from Jakarta Bay and Seribu Island waters. Most of them were oil degrading bacteria (41.98%) and the rest were PAH degrading bacteria. Oil pollution level may impact the number of strain of hydrocarbonoclastic bacteria isolated. Among the hydrocarbonoclastic bacteria isolated from Jakarta Bay and Seribu Islands, Alcanivorax, Marinobacter, Achromobacter and Bacillus were common hydrocarbonoclastic genera in Jakarta Bay and its surrounding waters. Alcanivorax spp. is important oil and PAH-degrader found not only in temperate waters, but in tropical waters as well.