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ISOLATION OF ESSENSIAL OIL OF NUTMEG (Myristica fragrans Houtt) and ANTIOXIDANT ACTIVITY TEST WITH DPPH Ginting, Binawati; Maira, Ratna; ., Mustanir; Helwati, Hira; Desiyana, Lydia Septa; Mujahid, Rohmat
Jurnal Natural Volume 18, Number 1, February 2018
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (628.444 KB) | DOI: 10.24815/jn.v18i1.6604

Abstract

The essential oil of the nutmeg plant (Myristica fragrans Houtt) has been obtained from roots, bark, fruit, mace and seeds using Stahl steam distillation. Each essential oil have tested for antioxidant activity with DPPH. Antioxidant activity of essential oil from each nutmeg plant to DPPH with concentration 25, 50 and 100 ppm. Each essential oil did not show strong antioxidant activity but the result of nutmeg isolation had strong antioxidant activity with IC50 that was 80,555 ppm. Based on GC-MS analysis of essential oil of nutmeg isolate obtained myristicin compound with 96.52% area and time Retention 22.127. Myristicin is one of the main components of essential oils of nutmeg plants that play an important role as antioxidants. Keywords: Nutmeg plant (Myristica fragrans Houtt), Essential Oil, DPPH, Antioxidant radicalsREFERENCES Suryanti, Siti. 2014. Metabolit Sekunder Pada Tanaman dan Fungsinya. http://seputarduniasains.blogspot.com Diakses tanggal 2 juni 2015. Gupta, A. D., Bansal, V. K., Babu, V., Maithil, N. 2013. Chemistry, antioxidant and antimicrobial potential of nutmeg (Myristica fregrans Houtt) Journal of Genetic Engineering and Biotechnologyh 11:25-31. Rodianawati, I., Hastuti, P., Cahyanto, M. N. 2015. Nutmeg’s (Myristica fragrans Houtt) Oleoresin: Effect of Heating to Chemical Compositions and Antifungal Properties Procedia Food Science 3:244-254. Masyithah, Z. 2006. Pengaruh Volume dan Konsentrasi Pelarut pada Isolasi Trimiristin dari Limbah Buah Pala Jurnal Teknologi Proses 5(1) Januari; 64-67. Wibowo, S dan Komarayati, S. 2015. Sifat Fisiko Kimia Minyak Cupresus (Cupressus benthamii) Asal Aek Nauli, Parapat Sumatera Utara Jurnal Penelitian Hasil Hutan 33 (2) Juni 2015: 93-103. Nurdjannah, N. 2007. Teknologi Pengolahan Pala. Badan Penelitian dan Pengembangan Pertanian. Bogor. Hellen, M, Vargheese, T.N, Kumari, J, Abiramy, Sajina, Sree, J, 2012, Phytochemical Analysis and Anticancer Activity of Essential Oil From Myristica fragrans, International Journal of Current Pharmaceutical Review and Research. Chatterjee, S, Zareena Niaz, S. Gautam, Soumyakanti Adhikari, Prasad S. Variyar, Arun Sharma, 2007, Antioxidant Activity of Some Phenolic Constituents from Green Pepper (Piper nigrum L.) and Fresh Nutmeg Mace (Myristica fragrans) J. Food Chemistry 101, 515–523. Sulaiman, S. F and Kheng, L. O. 2012. Antioxidant and anti food-borne bacterial activities of extracts from leaf and different fruit parts of Myristica fragrans Houtt. Food Control 25:533-536.Akinboro, A, Kamaruzzaman, M. B, Asmawi, M. Z, Sulaiman, S. F, Sofiman, O. H. 2011. Antioxidans in Aqueous extract of Mirystica fragrans (Houtt) Suppress Mitosis and Cyclophosphamide-induce chromosomal aberrations in Allium cepa L. Cells, Journal of Zhejiang University-SCIENCE B (Biomedicine Biotechnology). ISSN 1673-1581 (Print); ISSN 1862-1783 (Online).www.zju.edu.cn/jzus; www.springerlink.comGinting, B., 2013, Aktifitas Antifungi Ektrak Daun Pala (Mirystica fragrans Houtt), Prosiding Seminar Nasional Kimia tahun 2013. Ginting, B, T. Barus, P, Simanjuntak, L. marpaung., 2013, Isolasi dan Sifat Antioksidan Total Flavonoid Daun Pala (Mirystica fragrans Houtt), Prosiding Seminar Nasional Kimia, Samarinda Ginting, B, T. Barus, P, Simanjuntak, L. marpaung.,2013, Isolasi dan Dan Penentuan Aktivitas Antioksidan  Total Alkaloid Daun Pala (Mirystica fragrans Houtt), Prosiding Seminar Nasional Yusuf Banseh Ginting, B, T. Barus, P, Simanjuntak, L. marpaung., 2016, Isolation and Identification of Flavonoid Compound from Nutmeg Leaves (Mirystica fragrans Houtt), Asian Journal Of Chemistry. Ginting, B., Mustanir., Helwati, H., Desiyana, L. S., Eralisa, Mujahid, R. 2017.  Antioxidant Activity Of N-Hexane Extract Of Nutmeg Plants From South Aceh Province. Jurnal Natural 17(1) Ramaswany, V. N., Varghese, A., Simon. 2011. An Investigation on Cytotoxic Ternatea L International Journal of Drug Discovery 3: 74-77. ISSN : 0975-4423. Guenther, E. 1987. Minyak Atsiri Jilid 1, terjemahan dari: Essensial oil. Penerjemah: Ketaren S, Universitas Indonesia Press, Jakarta.Harborne, J.B. 1987. Metode Fitokimia: Penentuan Cara Moderen Menganalisa Tumbuhan. Terjemahan dari Phytochemical Methods oleh Kosasih Padmawinata. ITB. Bandung.Sipahelut, S. G. 2012. Karakteristik Minyak Daging Buah Pala (Myristica fragrans Houtt) Melalui Beberapa Cara Pengeringan Dan Distilasi Journal Agroforestri 7(1) Maret 2012.Andini, V., Gupta, S., Chatterejee, S., Variyar, P.S and Sharma, A. 2015. Activity Guided Characterization of Antioxidant Components from Essential Oil of Nutmeg (Myristica fragrans). Vol 52; 221-230.Nagja, T., Vimal, K, Sanjeev, A. 2015. Myristica fragrans: A Comprehensive Review International Journal of Pharmacy and Pharmaceutical Sciences 8 (2).Isnindar., Wahyuono, S., Setyowati, E.P. 2011. Isolasi dan Identifikasi Senyawa Antioksidan Daun Kesemek (Diospyros kaki Thunb.) dengan Metode DPPH (2,2-difenil-1-pikrilhidrazil). Majalah Obat Tradisional. 16(3), 157-164.Ramy, M., Fayed, S.A and Mahmoud, G.I. 2010. Chemical Compositions, Antiviral and Activities of Seven Essential Oils. Journal of Applied Sciences Research. 6(1); 50-62.Indriaty, F., Assah, Y., Mamahani, S.N. 2015. Serbuk minuman berbasis daging buah pala. Baristand. Manado.Morita, T., Jinni, K., Kawagishi, H., Arimoto, Y., Suganuma, H., Inakuma, T, and Sigiyama, K. 2003. Hepatoprotective Effect or Myristicin from Nutmeg (Myristica fragrans) on Lipopolisaccaride/d-galactosamine-induced Liver Injury. J. Agric. Food Chem. 15(6):1.560-1.565.Syarifuddin, I., Kaimudin, M., Torry, R.F., dan Biantoro, R. 2014. Isolasi Trimiristin Minyak Pala Banda Serta Pemanfaatannya Sebagai Bahan Aktif Sabun Jurnal Riset Industri 8(1); 23-31.
ANTIBACTERIAL ACTIVITIES OF PERFUME: COMBINATION FLOWER MAGNOLIA ALBA, CANANGA ODORATA AND MIMUSOPS ELENGI L, FIXED WITH POGOSTEMON CABLIN OIL Nasution, Rosnani; Azwar, Azalia Izdhihar; Helwati, Hira; Marianne
Indonesian Journal of Pharmaceutical and Clinical Research Vol 2 No 1 (2019): Indonesian Journal of Pharmaceutical and Clinical Research
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (787.644 KB) | DOI: 10.32734/idjpcr.v2i1.814

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Abstract: The making of perfume from the combination Magnolia  alba (M. alba) flower oil, Cananga odorata (C. odorata) and Mimusops elengi L (M. elengi) fixed with Pogostemon cablin oil (P. cablin) have been done. Essential oil of M. alba and C. odorata was distilled by water distillation and extraction essential oil of M. elengi flower using n-hexane evaporated solvent method. Composition perfume through organoleptic test obtained with a ratio of 4% v/v, 4% v/v, and 2% v/v for each essential oil M. alba, C. odorata and M. elengi. Antibacterial activity of the perfume and each of the volatile oil was performed by the disc diffusion method. Antibacterial activity test showed that perfumes were able to inhibit the growth of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) bacteria, but less active. Antibacterial activity of the perfume was more active against E. coli with an inhibitory zone of 8.3 mm and 39.81% inhibitory power  than S. aureus bacterial 7.3 mm inhibition zone with 37.55% inhibitory power. Perfumes after storage for  one, and two months had better antibacterial activity than before storage with an average inhibitory zone of 11-15 mm and inhibitory power of 50- 68,18%.   Keywords: Antibacterial, E. Coli, C. Odorata, M. alba, M. elengi, P. cablin, , S. aureus  
Isolation and characterization of cellulose from rice husk waste and sawdust with chemical method ANDALIA, RIZKI; RAHMI, RAHMI; JULINAWATI, JULINAWATI; HELWATI, HIRA
Jurnal Natural Volume 20 Number 1, February 2020
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (324.486 KB) | DOI: 10.24815/jn.v20i1.12016

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Cellulose had been isolated from rice husk waste and sawdust with chemical method through hydrolysis with HCl 2.5 N and bleaching process with  H2O23%.The yields were 13,45% and 22,75% (w/w) of cellulose from rice husk waste and sawdust, respectively. The chemical treatment caused partial removal of lignin and hemicellulose from raw material showed by FTIR spectrum.  Structure and morphology of cellulose from rice husk waste and sawdust were investigated using X-Ray Diffraction (XRD) and Scanning Electron Microscopy (SEM). The XRD patterns showed cellulose structure converted from amorphous to crystalline form after bleaching process while SEM images showed that the isolated cellulose from rice husk waste had rougher surface than cellulose from sawdust. The SEM micrograph also showed that the isolated cellulose from rice husk was in the form of aggregates.
Polymerization Domain Translated from 0.9 kb Gene Fragment of DNA Polymerase I from a Thermo-Halophilic PLS A Strain Iqbalsyah, Teuku Mohamad; Velayati, Muhammad A; Helwati, Hira; Febriani, Febriani
Jurnal Kimia Sains dan Aplikasi Vol 23, No 5 (2020): Volume 23 Issue 5 Year 2020
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2315.333 KB) | DOI: 10.14710/jksa.23.5.183-188

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The search for novel DNA Polymerases I, with higher fidelity and better polymerization rate, is essential to improve the Polymerase Chain Reaction method. A thermo-halophilic bacterium has been isolated from an undersea hot spring, dubbed Pria Laot Sabang (PLS) A strain. The 0.9 kb DNA Polymerase I gene fragments from the isolate were amplified, sequenced, and identified. The fragments were part of the polymerization domain of the enzyme. Homological analysis of the gene sequence showed that the PLS A strain was closely related to Bacillus caldolyticusstrain XM. However, Swissprot structural analysis reveals that PLS A strain had high homology to Geobacillus stearathermophilus. Full sequence analysis is still needed to identify the species and evaluate the intact enzyme structure.
ISOLATION OF ANTIOXIDANT COMPOUNDS OF METHANOL EXTRACT OF NUTMEG LEAVES (Myristica fragrans Houtt) Nurmilasari, Nurmilasari; Ginting, Binawati; Helwati, Hira
Jurnal Natural Volume 17, Number 1, March 2017
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.145 KB) | DOI: 10.24815/jn.v17i1.6998

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Abstract.The antioxidant activity from methanol extract of nutmeg leaves (Myristica fragrans Houtt) was performed using 2,2-diphenyl-1-picrylhydrazil (DPPH). Antioxidant assay using DPPH of the methanol extract at a concentration of 25 ppm, 50 ppm and 100 ppm, showed high antioxidant activity with IC50 = 36.31 ppm and Vitamin C showed antioxidant activity with IC50 = 3.657 ppm. The methanol extract of nutmeg leaves were partitioned with chloroform to obtain chloroform extract of the leaves with IC50 = 28.30 ppm. isolation of the active compounds from the chloroform  extract of the nutmeg leaf using column chromatography yielded 6 mix fractions MFMD 1, MFMD 2, MFMD 3, MFMD 4, MFMD 5 and MFMD 6. The test results of antioxidant activity in very strong category is fractions MFMD 4, MFMD 3, MFMD 2, MFMD 6 and MFMD 5 with IC50 of each are 26.590 ppm, 27.239 ppm, 29.639 ppm, 39.766 ppm and 55.436 ppm, while antioxidant activity in a weak catagory is fraction MFMD 1 with IC50 is 126.270 ppm. This shows that the fraction of the leaf has a better inhibitory activity, because some of the active compound contained in the fraction can synergize in inhibiting free radicals, so that the IC50 value of the fraction is stronger than the extract. Keywords: Antioxidant, chromathography, Nutmeg (Myristica fragransHoutt), methanol extract, leaves
EFFECT OF PLASTICIZERS ON MECHANICAL PROPERTIES OF EDIBLE FILM FROM JANENG STARCH – CHITOSAN Juandi, Narlis; Rahmi, Rahmi; Helwati, Hira
Jurnal Natural Volume 16, Number 2, September 2016
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.468 KB) | DOI: 10.24815/jn.v16i2.5125

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The interest in the development of edible and biodegradable films has increased because it is every day more evident that non degradable are doing much damage to the environment. In this research, edible films were based on blends of janeng starch in different proportions, added of palm oil or glycerol, which were used as plasticizers. The objective was to study the effect of two different plasticizers, palm oil and glycerol of edible film from janeng starch–chitosan on the mechanical properties and FTIR spectra. Increasing concentration of glycerol as plasticizer resulted tend to increased tensile strength and elongation at break. The tensile strength and elongation at break values for palm oil is higher than glycerol as plasticizer at the same concentration. FTIR spectra show the process of making edible film from janeng starch–chitosan with palm oil or glycerol as plasticizers are physically mixing in the presence of hydrogen interactions between chains.
ANTIMICROBIAL ACTIVITY OF METHANOL EXTRACT FROM STEM BARK OF Cinnamomum sintoc Saidi, Nurdin; Helwati, Hira; Lubis, Lailatul Qhadariah; Bahi, Muhammad
Jurnal Natural Volume 17, Number 2, September 2017
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (154.546 KB) | DOI: 10.24815/jn.v17i2.8049

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Antimicrobial activity of methanol extract from stem bark of Cinnamomum sintoc has been evaluated against Candida albicans, Staphylococcus aureus and Escherichia coli. The extraction of compound was carried out by maceration, then isolation by column chromatograph, which yielded five (5) subfractions (A-E). Activity against fungus C. albicans, S. aureus bacteria dan E. coli using agar dilution method in paper disk. Methanol extract was not potent against antifungal activity but shows antibacterial activity with medium category. Subfraction C showed that antibacterial activity against S. aureus and E. coli with weak category, but subfractions D and E did not show any activity.
ANTIMICROBIAL ACTIVITY OF n-HEXANE EXTRACTS OF RED FRANGIPANI (Plumeria rocea) Ali Husni, Muhammad; Murniana, Murniana; Helwati, Hira; Nuraini, Nuraini
Jurnal Natural Volume 13, Number 1, March 2013
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.423 KB)

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Antimicrobial assay of n-hexane plant extracts against Escherichia coli and Staphylococcus aureus had been done. Extraction with n-Hexane of the flowers yielded 2,08% of extract, while leaves and stems yielded 3,21% and 2,19% of n-hexane extracts, respectively. The extracts showed different activity against both bioindicators. The leaves extracts showed the strongest activity indicated by wider diameters of inhibition zone, 19,7 mm against E. coli and 13,3 mm against S. aureus at 10% of the extracts’ concentration. At the same concentration (10%), the steam and flower extracts showed less inhibitory activities with  11,3 mm and 8,3 mm against E. coli, and 12,0 mm and 11,0 mm against S. aureus. At the lowest concentration of the extracts, the leaves extracts showed the strongest activity against E. coli (14,3 mm) while the steam extracts showed highest inhibitory activity against S. aureus ( 7,7 mm). Based on antimicrobial assay, it was suggested that the leaves extracts demonstrated strongest activity than other extracts. It was assumed that leaves extracts contained more secondary metabolite than flowers and steams, and the leaves have phenol compounds which are not present in other extracts.
ANTIOXIDANT ACTIVITY OF N-HEXANE EXTRACT OF NUTMEG PLANTS FROM SOUTH ACEH PROVINCE Ginting, Binawati; Mustanir, Mustanir; Helwati, Hira; Desiyana, Lydia Septa; Eralisa, Eralisa; Mujahid, Rohmat
Jurnal Natural Volume 17, Number 1, March 2017
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.588 KB) | DOI: 10.24815/jn.v17i1.6969

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Abstract. It has tested the antioxidant activity of n-hexane extract respective roots, bark, fruit and seed crops of nutmeg against DPPH. Each extracted by maceration method with n-hexane. The extraction of each plant nutmeg with n-hexane extract obtained yield of n-hexane respectively 8,35%; 81,5%; 11,89% and 55,63%. The result of the antioxidant activity of n-hexane extract of each plant nutmeg against DPPH with a concentration of 25 ppm, 50 ppm and 100 ppm is obtained IC50, respectively, are 0,216 ppm, 63,755 ppm, 43,998 ppm and 11,599 ppm and positive control vitamin C ( IC50 = 3,657 ppm). Vitamin C is a standard compound is more often used than butyl for very high antioxidant activity. The antioxidant activity increases from high to low at the roots, seeds, fruits and bark of plants nutmeg in a row. Based on data GC-MS chemical components of each successive extracts of roots, bark, fruit and seeds are 41, 41, 44, 43 components of secondary metabolites. The main components of each part of the plant based on data from GC-MS are a 5-Octadecanoic acid, linalool, eugenol, myristicin, methoxyeugenol. The compounds are thought to play an active role in inhibiting free radicals from DPPH. n-Hexane extract nutmeg plant potential as an antioxidant. Keywords:Nutmeg (Myristica fragrans Houtt), n-hexane extract, Antioxidant, DPPH
Isolation Of Antioxidant Compounds Of N-Hexane Extract Of Nutmeg (Myristica Fragrans Houtt) Leaves Ginting, Binawati; Hadijah, Siti; Helwati, Hira
JURNAL KIMIA MULAWARMAN Vol 15 No 1 (2017)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jkm.v15i1.584

Abstract