Hendra Herdian
Bagian Pakan dan Nutrisi Ternak, Balai Pengembangan Proses dan Teknologi Kimia (BPPTK) – LIPI Jln. Jogja-Wonosari Km. 31,5, Gading, Playen, Gunung Kidul, Yogyakarta.

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Effect of Morinda citrifolia leaf as saponin sources on fermentation characteristic, protozoa defaunated, gas and methane production of ruminal fluid in vitro Herdian, Hendra; Istiqomah, L.; Febrisiantosa, A.; Setiabudi, D.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 2 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (85.186 KB) | DOI: 10.14334/jitv.v16i2.639

Abstract

Many studies have reported that the Morinda citrifolia (pace plant) was a useful material for human health. However the exploration of this plant on rumen fermentation is still needed. Therefore, a research was done to study the effect of M. citrifolia leaf on fermentation characteristics of rumen fluid consisted of protozoa defaunated process, VFA composition, NH3 content, rumen microbial protein content, gas and methane production using in vitro techniques. Rumen fluid obtained from two fistulated Ongole crossbreed cattle fed with forage and concentrate feed ration (70 : 30). The fluid was incubated at 39ºC for 48 hours. The treatment on the rumen fluid consisted of control treatment: 100% (200 mg DM) kolonjono forage substrate (Penisetum purpureum) and M. citrifolia treatments: kolonjono forage plus M. citrifolia (equivalent saponin) 3; 6; 9; and 12 mg DM, respectively. The treatment of M. citrifolia leaf addition showed declined patterns in the number of protozoa population (P < 0.05) compared to control. There was no effect of M. citrifolia treatment on VFA composition and NH3 content (P > 0.05). Microbial protein content in rumen fluid increased (P < 0.05) by M. citrifolia treatments compared to control. Effect of M. citrifolia treatments on the gas production was not significantly different (P > 0.05) compared to control, while M. citrifolia treatments reduced the methane gas production of (P < 0.05) compared to control. It was concluded that M. citrifolia leaf has potential as a limiting agent of protozoa population and methane gas production in rumen. Key words: M. citrifolia, Defaunated, Saponin, In Vitro
PREVALENSI KOLIBASILOSIS PADA AYAM BROILER YANG DIINFEKSI Escherichia coli DENGAN PEMBERIAN BIOADITIF, PROBIOTIK, DAN ANTIBIOTIK Suryani, Ade Erma; Karimy, Mohammad Faiz; Istiqomah, Lusty; Sofyan, Ahmad; Herdian, Hendra; Wibowo, Michael Haryadi
Widyariset Vol 17, No 2 (2014): Widyariset
Publisher : LIPI-Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1657.114 KB)

Abstract

The purpose of this research was to study the efficacy of bio-additive (a mixture of Lumbricus rubellus meal extract, Morinda citrifolia leaf extract and lactic acid bacteria ), probiotics, and antibiotics on the prevalence co- libacillosis and healthy status of broiler that infected by E. coli strain Avian Pathogenic Escherichia coli (APEC). A total of 140 DOC were distribute randomly into 20 units of cage, each filled with 7 chickens were arranged in a completely randomized desig . Twenty cages were divided into 5 group , each treatment consisted of 4 replicates. The treatment group consisted of treatment A = infection of E. coli positive control), B = infection of E. coli + bio- additiv , C = infection of E. coli + probiotic , D = infection of E. coli + antibiotic , E = no E. coli infection negative contro). Feed base on corn- soybean is formulated as a basal fee . The experiments were conducted for 35 days, on day 21 chickens infected E. coli. ND vaccination is given at the age of four days and 15 days. The observed parameters were changes of macroscopic, isolation and identification of E. coli, changes in histopathology, blood profiles and antibody titer against ND. Results showed the prevalence colibasillosis on treatment B resulted in the lowest rate (33.3), results in the isolation and identification of chicken with positive clinical symptoms kolibasilosis infected APEC, and microscopic observations showed histopathological changes in the organs pancreas, heart, liver, and exchanges fabrisius lung. The results of the blood profile analysis showed the presence of the body’s defense mechanism against bacterial infectio , which is evident from the number of leukocytes in treatment A and C are higher tha treatment E (P> 0.0 ), red cell count treatment D higher than E treatment (P> 0.0 ), and total of Hb treatment A higher than treatment E P> 0.0 ). Based on the overall health status, it can be concluded that the administration of bioaditif decrease the prevalence o  colibasillosis 67.7 % .
Effect of Morinda citrifolia leaf as saponin sources on fermentation characteristic, protozoa defaunated, gas and methane production of ruminal fluid in vitro Herdian, Hendra; Istiqomah, L.; Febrisiantosa, A.; Setiabudi, D.
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 2 (2011): JUNE 2011
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (85.186 KB) | DOI: 10.14334/jitv.v16i2.639

Abstract

Many studies have reported that the Morinda citrifolia (pace plant) was a useful material for human health. However the exploration of this plant on rumen fermentation is still needed. Therefore, a research was done to study the effect of M. citrifolia leaf on fermentation characteristics of rumen fluid consisted of protozoa defaunated process, VFA composition, NH3 content, rumen microbial protein content, gas and methane production using in vitro techniques. Rumen fluid obtained from two fistulated Ongole crossbreed cattle fed with forage and concentrate feed ration (70 : 30). The fluid was incubated at 39ºC for 48 hours. The treatment on the rumen fluid consisted of control treatment: 100% (200 mg DM) kolonjono forage substrate (Penisetum purpureum) and M. citrifolia treatments: kolonjono forage plus M. citrifolia (equivalent saponin) 3; 6; 9; and 12 mg DM, respectively. The treatment of M. citrifolia leaf addition showed declined patterns in the number of protozoa population (P < 0.05) compared to control. There was no effect of M. citrifolia treatment on VFA composition and NH3 content (P > 0.05). Microbial protein content in rumen fluid increased (P < 0.05) by M. citrifolia treatments compared to control. Effect of M. citrifolia treatments on the gas production was not significantly different (P > 0.05) compared to control, while M. citrifolia treatments reduced the methane gas production of (P < 0.05) compared to control. It was concluded that M. citrifolia leaf has potential as a limiting agent of protozoa population and methane gas production in rumen. Key words: M. citrifolia, Defaunated, Saponin, In Vitro
PHYSICAL AND CHEMICAL PROPERTIES OF ORGANIC MINERAL ADDITIVE FOR RUMINANT THROUGH BIOLOGICALLY INCORPORATED BY SACCHAROMYCES CEREVISIAE IN DIFERENCE SUBSTRATES Sofyan, Ahmad; Herdian, Hendra; Sakti, Awistaros Angger; Khairulli, Gumilang; Jayanegara, Anuraga
Jurnal Kimia Terapan Indonesia (Indonesian Journal of Applied Chemistry) Vol 17, No 2 (2015)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (432.832 KB) | DOI: 10.14203/jkti.v17i2.30

Abstract

This research was conducted to evaluate physical and chemical properties of organic mineral additive incorporated by Saccharomyces cerevisiae which was cultivated on different growth media consisted of cassava flour and rice bran, respectively. Treatment was arranged on completely randomized design consisted of rice bran without inoculants (RBo), rice bran with inoculants (RBi), cassava flour without inoculants (CFo), cassava flour with inoculants (CFi). Substrates were fortified by microminerals contained of Cu (100 ppm), Mn (100 ppm), Zn (100 ppm), I (10 ppm), Fe (2.5 ppm) and Co (2 ppm). Inoculation of S. cerevisiae could reduce fungal contamination. Physical characteristic of cassava flour was better in flavour and texture than rice bran in which tends to rancidity. Nutrients composition was similar in cassava and rice bran however crude fibre content in rice bran tends to be higher after fermentation. Mineral content (Zn and Fe) relatively decreased and in substrate supplemented by inoculant and rice bran had higher than cassava. In summary, micromineral was incorporated in cassava flour with inoculated S. cerevisiae had better than rice bran.Keywords: Organic mineral, cassava flour meal, S. cerevisiae, rice bran, ruminant.
PROFIL ASAM AMINO EKSTRAK CACING TANAH (LUMBRICUS RUBELLUS) TERENKAPSULASI DENGAN METODE SPRAY DRYING Hayati, Septi Nur; Herdian, Hendra; Damayanti, Ema; Istiqomah, Lusty; Julendra, Hardi
Teknologi Indonesia Vol 34 (2011)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (16.048 KB) | DOI: 10.14203/jti.v34iKhusus.33

Abstract

Antibiotics in poultry were widely used as growth promotor and anti-infective. When antibiotics were used continuously for long periods, it caused microbial resistance. Various attempts were made to replace antibiotics along with the increasing of trend towards demand for healthy, safe and free from harmful residues of livestock products. One of them was application of earthworm which contains high protein and complete amino acids. Earthworms were extracted into water extract form by decoct method. Water extract form had some weaknesses. It was necessary to formulate into a solid dosage form which was more stable, reproducible, and practical. The selected formulation was encapsulation by spray drying method. Quality control of feed additives can be seen from biological value. Biological value correlated positively with amino acids equilibrium or Essential Amino Acid Index (EAAI). The result showed that earthworm extract had both essential and non-essential amino acids higher than earthworm meal and encapsulated earthworm extract. The highest essential amino acid in earthworm meal was isoleucine (3.14%), in earthworm extract was lysine (8.16%), and in encapsulated earthworm extract was leucine (1.71%). The highest non-essential amino acids in earthworm meal and encapsulation of earthworm extract were glutamic acid, respectively 7.67% and 1.87%, while the earthworm extracts was serine (14.52%). The high value of IAAE in earthworm extract showed that the extraction method resulted better amino acid balance (69.87%) than earthworm meal (58.67%). While the value IAAE of encapsulated earthworm extract showed that levels of essential amino acid balance was lower (16.05%) than the earthworm meal (69.87%) and the earthworm extract (58.67%).