Tinny Endang Hernowati
Laboratorium Patologi Klinik Fakultas, Kedokteran Universitas Brawijaya

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Respon Proliferasi, Diferensiasi dan Ekspresi C/EBPa Akibat Paparan Quercetin pada Kultur Preadiposit Tikus (Rattus Norvegicus) Strain Wistar Secara In Vitro Ratnawati, Retty; Satuman, Satuman; Hernowati, Tinny Endang
Research Journal of Life Science Vol 2, No 1 (2015)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (603.856 KB) | DOI: 10.21776/ub.rjls.2015.002.01.4

Abstract

Introduction. Obesity is a metabolic syndrome as risk factor to the cardiovascular disease. Obesity occurs caused by adipocyte massive that is started by proliferation and differentiation of preadipocytes that involves transcription factors such as C/EBPα ,PPARγ and SREBP-1. Is known the TNF α and [Ca2+]i Increase when adiponectin decrease at the end of adipocyte differentiation. Therefore it is a deemed treat the obesity through the inhibition of adipocyte proliferation and differentiation using natural resources. Quercetin in due of the flavonoid found in apple,vegetables etc. The aim of this study is to prove the affectivity of quercetin to inhibit proliferation and differentiation of preadipocyte through inhibited of C/EBPα in rat preadipocytes culture method.Methods. Their study was a laboratory experimental. Quercetin was exposed to the preadipocytes human culture, after it was induced by differentiation stimulator the doses quercetin was grouped in: control, 50 µM (Q50), 125 µM (Q125) and 625 µM (Q625) groups. The amount of proliferation and differentiation was descriptive analyzed. The expression of C/EBPα, were identified by Immunocytochemistry and western blotting.Result. Quercetin 100 inhibited the rate of proliferation cell, while quersetin 625 inhibited differentiation in preadipocytes culture. There was a dose dependent to quersetin towards the decrease of PPARγ expression and C/EBPα expression in this preadipocytes culture.Conclusion. It is conclusion that quersetin inhibits proliferation and differentiation preadipocytes culture through the decrease of the expresion of C/EBP α.
NILAI DIAGNOSTIK UJI TROPONIN I KUANTITATIF METODE IMMUNOKROMATOGRAFI Fatonah, Siti; Widijanti, Anik; Hernowati, Tinny Endang
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 14, No 1 (2007)
Publisher : PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v14i1.920

Abstract

Cardiac troponins are the most sensitive and specific biochemical markers of myocardial damage but there is no standardization of WHO for cardiac troponin I, resulting in a variability for diagnostic value. It is necessary to determine diagnostic value for a new kitof troponin I. To evaluate a new quantitative immunochromatography assay for troponin I at a various cut off level. A cross sectionalstudy was conducted in 64 patients with acute myocardial infarction (AMI) and 55 non-AMI as control from February to September2007. The level of cardiac troponin I (cTnI) was measured and determined it diagnostic value at a various cut off level. The sensitivity,specificity, PPV and NPV of this assay were 91%, 91%, 92% and 89% at cut off level of 1,0 ng/ml (according to the kit), respectively.The cut off of cTnI were divided into five levels: 0.8, 1.0, 1.2, 1.5, and 2.0 with the area under curve were 0.923, 0.908, 0.912, and0.897, respectively. The sensitivity were 94%, 91%, 86%, 81% and 72%, respectively, the specificity were 91%, 91%, 96%, 98% and98%, respectively. This rapid diagnostic test is sensitive and specific to diagnose an acute coronary syndromes.