Bethy Suryawathy Hernowo
Universitas Padjadjaran

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TRIPLE NEGATIVE BREAST CANCER CHARACTERISTICS BASED ON BASAL-LIKE AND NON-BASAL-LIKE SUBTYPES Akwarini, Fifi; Fadjari, Trinugroho Heri; Hernowo, Bethy Suryawathy
International Journal of Integrated Health Sciences Vol 7, No 1 (2019)
Publisher : International Journal of Integrated Health Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/ijihs.v7n1.1570

Abstract

Objective: To observe triple negative breast cancer (TNBC) characteristics in three hospitals located in Bandung based on basal-like (BL) and non-basal-like (NBL) subtypes.Methods: This was a cross-sectional study which used descriptive categorical data from medical records and paraffin blocks of TNBC patients treated in Dr. Hasan Sadikin General Hospital, Bandung; Borromeus Hospital; and Santosa Hospital Bandung Central in the period of January 1, 2012?December 31, 2016. The subjects of the study were 57 TNBC patients. The data collected in the study based on medical records were age, tumor size, histopathological images, severity, and immunohistochemical data. The paraffin blocks of the patients based on the completed medicals records were investigated through examinations of immunohistochemichal cytokeratin (CK) 5/6 expressions and epidermal growth factor receptor (EGFR).Results: Prevalence of TNBC were 82.5% of basal-like subjects and 17.5% of non-basal-like subjects. Among the TNBC subjects, median age of each subtype was 50 years of basal-like subtype and 45 years of non-basal-like subtype. Both subtypes were mostly found in the subjects who aged >40 years. Higher histopathological grade was discovered in both subtypes. The therapy mostly carried out to the subjects was adjuvant chemotherapy. Majority of basal-like subtype subjects were still alive and had longer survival rate and lower incidences of deaths when compared to the non-basal-like subtype.Conclusion: In TNBC, the basal-like subjects showed greater median age, lower severity stage, and longer survival rate than the non-basal-like subjects. There was no histopathology grade between both subtypes. Keywords: Basal-like and non-basal-like subtypes, breast cancer, characteristics, triple negative 
KORELASI ANTARA EKSPRESI AROMATASE, FOLLICLE STIMULATING HORMONE (FSH), DAN INSULIN-LIKE GROWTH FACTOR (IGF)-I DENGAN MATURITAS OOSIT PADA PELAYANAN TEKNOLOGI REPRODUKSI BERBANTU (TRB) Guyansyah, Assangga; Biben, Achmad; Mose, Johanes C.; Hernowo, Bethy Suryawathy
Indonesian Journal of Applied Sciences Vol 2, No 1 (2012)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijas.v2i1.2729

Abstract

Karakteristik biokimia cairan folikel disekitar oosit memainkan peran penting dalam menentukan kualitas oosit yang padaakhirnya dapat meningkatkan keberhasilan program TRB. Secara umum mekanisme maturitas oosit yang dipengaruhi oleh unsur kimia cairan folikel melibatkan IGF-I yang bekerja dengan melipatgandakan efek FSHR, lalu menginduksi peningkatan kadar cAMP intraseluler yang merupakan mediasi utama stimulasi FSH pada ekspresi aromatase. Penelitian ini merupakan studi analitik korelasional dengan pendekatan crossectional pada pasien yang mengikuti program TRB di Klinik Aster Rumah Sakit Dr. Hasan Sadikin Bandung dalam kurun waktu Januari 2011 sampai dengan Februari 2012. Analisis hasil dilakukan menggunakan uji Shapiro Wilk, uji Wilcoxon, uji Mann-Whitney, uji ANAVA, rank Spearman, dan uji chi-kuadrat. Kemaknaan hasil uji akan ditentukan berdasarkan nilai p < 0,05. Pada penelitian ini didapatkan perbandingan jumlah oosit matur dan imatur pada ekspresi FSH kuat menunjukkan ada perbedaan yang bermakna (p=0,03). Analisis hubungan antara ekspresi IGF-I dengan maturitas oosit menunjukkan hasil yang tidak bermakna secara statistik (p=0,192). Didapatkan pula median proporsi prevalensimaturitas sebesar 0,71. Maturitas oosit dengan median rasio prevalensi > 0,71 pada ekspresi FSH kuat besarnya 1,71 kali bila dibandingkan dengan ekspresi FSH negatif. Pada ekspresi aromatase sedang dengan median rasio prevalensi maturitas > 0,71 sebesar 1,55 kali bila dibandingkan dengan ekspresi aromatase yang negatif.
PERBEDAAN IMUNOEKSPRESI CD10 DAN MMP9 ANTARA HIPERPLASIA ENDOMETRIUM TIPE ATIPIK DAN KARSINOMA ENDOMETRIOID YANG BERDIFERENSIASI BAIK Bahat, Lely S; Dewayani, Birgitta M; Afiati, Afiati; Hernowo, Bethy Suryawathy
JAMBI MEDICAL JOURNAL "Jurnal Kedokteran dan Kesehatan" Vol 6 No 2 (2018): JAMBI MEDICAL JOURNAL Jurnal Kedokteran Dan Kesehatan
Publisher : FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (778.36 KB) | DOI: 10.22437/jmj.v6i2.5952

Abstract

Background: Endometrial hyperplasia is one of the malignancy triggers for endometrium. Based on histopathologic features, it is divided into two types: atypical and non-atypical. Endometrial carcinoma is one of the gynecological malignancies, ranked 5th the world for its malignancy. Endometrial carcinoma is divided into two types, type I (endometrioid), and type II (non endometrioid). In histopathology, particularly in the sample of tissue curettage process, It is difficult to distinguish between endometrial hyperplasia atypical and well-differentiated endometrioid carcinoma. CD10 plays a role for homeostasis in extracellular matrix (ECM). If there is regulation disruption, it can lead to carcinogenesis. Whilst, MMP9 plays its role in the occurrence of invasion and metastasis. The aim of this study is to investigate the immunoexpression differences of CD10 and MMP9 in atypical endometrial hyperplasia and well-differentiated endometrioid carcinoma. So, this study can be used to determine a more accurate diagnosis especially on the tissue curettage sample. Methods: This study is an observational study by using cross sectional design. Total sample was 52 cases from hysterectomy which are consist of 26 cases atypical endometrial hyperplasia and 26 cases well-differentiated endometrioid carcinoma. Those samples and cases were obtained from the Department of Anatomical Pathology in three hospitals in Bandung: Hasan Sadikin, Advent and Santosa. Therefore, the whole samples were marked by immunohistochemical &nbsp;CD10 and MMP9. Results: The results of this study found a statistically, significant differences between immunoexpression of CD10 and MMP9 with atypic endometrial hyperplasia and well-differentiated endometrioid carcinomas. The p values for CD10 and MMP9 are 0.003 and 0.008, respectively. Atypical endometrial hyperplasia and well-differentiated endometrioid carcinomas are significantly influenced by CD10 and MMP9, CD10 immunoexpression is the most influential variable.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Conclusion: Based on the results of this study it can be concluded that Immunoxpression of CD10 and MMP9 can be used as an immunohistochemical marker to differentiate atypical endometrial hyperplasia from well-differentiated endometrioid carcinoma, particularly on tissue curettage sample. Keywords: CD10, MMP9, endometrial hyperplasia atypic type, well-differentiated endometrioid carcinoma.
PERANAN CYCLIN D1 DAN VEGF TERHADAP RESPONS RADIOTERAPI PADA KARSINOMA NASOFARING TIDAK BERDIFERENSIASI STADIUM LANJUT Nurhidayat, Ade Apon; Afiati, Afiati; Usman, Hermin Aminah; Hernowo, Bethy Suryawathy
JAMBI MEDICAL JOURNAL "Jurnal Kedokteran dan Kesehatan" Vol 6 No 2 (2018): JAMBI MEDICAL JOURNAL Jurnal Kedokteran Dan Kesehatan
Publisher : FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (500.382 KB) | DOI: 10.22437/jmj.v6i2.5949

Abstract

Background: Nasopharyngeal carcinoma has a high incidence and mortality rate in Southeast Asia and Indonesia. Radioresistance is a major obstacle to successful treatment of nasopharyngeal carcinoma. DNA repair in the cell cycle and angiogenesis factors affects the response of tumor cells to radiotherapy. Cyclin D1 that functions in the cell cycle process and VEGF as an angiogenesis factor are considered to play a role in the occurrence of radioresistance. The objective of this study is to find the association between immunoexpression of Cyclin D1 and VEGF with radiotherapy response in undifferentiated nasopharyngeal carcinoma. Methods: This study used a retrospective case control analysis design, secondary data from medical records of patients diagnosed as undifferentiated nasopharyngeal carcinoma who received complete radiotherapy at the Radiation Oncology Department Dr Hasan Sadikin Bandung were taken. There were 44 samples divided into radiosensitive (22 samples) and radioresistant (22 samples) groups. Immunohistochemical examination of Cyclin D1 and VEGF was performed on paraffin blocks of patients' nasopharyngeal biopsy. Data analysis using Chi-Square test with p ?0.05 and OR 95% CI. Results: Cyclin D1 expressed strongly in 86.4% of the radioresistant group and 59.1% in the radiosensitive group (p&lt;0.05) and the OR 4,385 (0.993-19.356), VEGF was strongly expressed in 77.3% of the radioresistant group and 54.5% in the radiosensitive group (p&gt;0.05). Conclusion: There were significant association between Cyclin D1 with radiotherapy response in undifferentiated nasopharyngeal carcinoma. The stronger immunoexpression of Cyclin D1, the higher likelihood of radioresistancy. VEGF immunoexpression showed no significant association with radiotherapy response. Key words: cyclin d1, nasopharyngeal carcinoma,radiotherapy, VEGF
The role of cyclin D1 and vascular endothelial growth factor (VEGF) in radiotherapy response of undifferentiated nasopharyngeal carcinoma Nurhidayat, Ade Apon; Afiati, .; Usman, Hermin Aminah; Hernowo, Bethy Suryawathy
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 4 (2018)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (844.579 KB) | DOI: 10.19106/JMedScie/005004201802

Abstract

Nasopharyngeal carcinoma (NPC) has a high incidence and mortality rate in SoutheastAsia included in Indonesia. Radioresistance is a major obstacle for a successful treatmentof NPC. DNA repair in the cell cycle and angiogenesis factors affect the response oftumor cells to radiotherapy. Cyclin D1 that functions in the cell cycle process and vascularendothelial growth factor (VEGF) as an angiogenesis factor are considered to play arole in the occurrence of radioresistance. The objective of this study was to evaluatethe association between cyclin D1 and VEGF expressions with radiotherapy responsein undifferentiated NPC. This study used a retrospective case control analysis design.Secondary data from medical records of patients diagnosed as undifferentiated NPC whoreceived a complete radiotherapy at the Department of Radiation Oncology, Dr. HasanSadikin General Hospital, Bandung. There were 44 samples divided into radiosensitive(22 samples) and radioresistant (22 samples) groups. Immunohistochemical examinationof cyclin D1 and VEGF expressions was performed on paraffin blocks of patients?nasopharyngeal biopsy. Data analysis used Chi-Square test with p ?0.05. Cyclin D1 wasexpressed strongly in 86.4% of the radioresistant group and 59.1% in the radiosensitivegroup (p<0.05), VEGF was strongly expressed in 77.3% of the radioresistant group and54.5% in the radiosensitive group (p>0.05). In conclusion, there is significant associationbetween cyclin D1 expression with radiotherapy response in undifferentiated NPC.However, there is no association between VEGF expression with radiotherapy response.
The Role of Fibroblast Growth Factor Receptor 3 (FGFR3) and Androgen Receptor (AR) in a Non-invasive Urothelial Carcinoma Recurrences Dewi, Oki Meilani; Suryanti, Sri; Hernowo, Bethy Suryawathy
Global Medical & Health Communication (GMHC) Vol 7, No 2 (2019)
Publisher : Global Medical & Health Communication (GMHC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v7i2.3956

Abstract

Urothelial carcinoma is a bladder carcinoma that took place in the urinary tract. Non-invasive urothelial carcinoma patients have high recurrence rates (50?70%). The recurrences took so many years that may lead to the high-cost treatment and low survival rate. Fibroblast growth factor receptor 3 (FGFR3) and androgen receptor (AR) known to play a role in non-invasive urothelial carcinoma and potentially act as a prognostic marker to predict recurrences. This study aimed to discover the role of FGFR3 and AR in recurrences of non-invasive urothelial carcinoma. This research used a case-control study design. Samples took from patients diagnosed with non-invasive urothelial carcinoma registered at Dr. Hasan Sadikin General Hospital Bandung 1 January 2010?30 December 2015 period. Sixty samples consisted of 30 recurrent groups, and 30 non-recurrent groups individually fixated and embedded to paraffin block for FGFR3 and AR immunohistochemistry analysis. Analysis chi-square performed with a level of confidence 95% and statistical power 95%. p values<0.05 were considered to be statistically significant. Statistical analysis showed that FGFR3 immunoexpression was found significantly low on the recurrence group (p=0.002, OR=5.50). While AR immunoexpression was found insignificant (p=1.000, OR=1.00). FGFR3 immunoexpression from samples in the recurrent group with multiple tumors found to be significantly low (p=0.031, OR=6.067). This study showed that recurrences took place when FGFR3 lowly expressed within non-invasive urothelial carcinoma samples with multiple tumors. This finding may raise a candidate to early-predict the recurrence, thus will suggest early therapy.PERANAN FIBROBLAST GROWTH FACTOR RECEPTOR 3 (FGFR3) DAN RESEPTOR ANDROGEN (RA) TERHADAP KEJADIAN REKURENSI PADA KARSINOMA UROTELIAL BULI NON-INVASIFKarsinoma urotelial merupakan karsinoma buli yang sering terjadi pada saluran kemih. Karsinoma urotelial dibagi menjadi karsinoma urotelial non-invasif dan invasif. Pasien karsinoma urotelial non-invasif mempunyai kejadian rekurensi tinggi (50?70%) dan membutuhkan waktu lama untuk memantau kejadian rekurensi sehingga membutuhkan biaya tinggi dengan angka ketahanan hidup rendah. Fibroblast growth factor receptor 3 (FGFR3) dan reseptor androgen (RA) berperan dalam terjadinya karsinoma urotelial non-invasif dan berpotensi sebagai penanda prognostik yang memprediksi rekurensi secara akurat. Tujuan penelitian ini mengetahui peranan FGFR3 dan RA terhadap kejadian rekurensi pada karsinoma urotelial non-invasif. Penelitian menggunakan rancangan case-control study. Sampel berupa blok parafin yang diagnosis sebagai karsinoma urotelial non-invasif di RSUP Dr. Hasan Sadikin Bandung periode 1 Januari 2010?30 Desember 2015. Sebanyak 60 sampel dievaluasi terdiri atas 30 sampel kelompok rekurensi dan 30 kelompok tidak rekurensi. Pemeriksaan imunohistokimia menggunakan antibodi FGFR3 dan RA. Analisis menggunakan uji chi-square dengan taraf kepercayaan 95% dan kuasa uji (power test) 95%. Nilai p<0,05 dianggap signifikan secara statistik. Pada analisis statistik, imunoekspresi FGFR3 rendah signifikan pada kelompok rekurensi (p=0,002; OR=5,50) dan imunoekspresi RA tidak signifikan (p=1,000; OR=1,00). Imunoekspresi FGFR3 rendah dengan tumor multipel signifikan pada kelompok rekurensi (p=0,031;  OR=6,067). Hasil penelitian menunjukkan bahwa rekurensi terjadi ketika FGFR3 terekspresi rendah pada sampel karsinoma non-invasif dengan tumor multipel. Hal ini dapat menjadi penanda memprediksi kejadian rekurensi sehingga dapat dilakukan terapi yang lebih cepat.