Articles

Found 33 Documents
Search

EMBRIOGENESIS DAN DESIKASI EMBRIO SOMATIK JERUK KEPROK BATU 55 (CITRUS RETICULATA BLANCO.) UNTUK MENINGKATKAN FREKUENSI PERKECAMBAHAN Fathur Rahmi, Atika; Purwito, Agus; Husni, Ali; Dinarti, Diny
Jurnal Hortikultura Indonesia Vol. 8 No. 2 (2017): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (381.578 KB) | DOI: 10.29244/jhi.8.2.79-87

Abstract

ABSTRACTIn vitro breeding technique of citrus is effective when optimum explant regeneration method is obtained. Low germination frequency and high abnormality were barrier in citrus somatic embryogenesis. This research aimed at optimizing somatic embryogenesis in Tangerine var. Batu 55. This research consisted of 3 experiments. First experiment was maturation of embryogenesis, using Completely Randomized Design (CRD) method. Modified MS+MW was used as basic media added with 500 mg L-1 malt extract (control) and addition of 3 mg L-1 BAP, and 2.5 mg L-1 ABA as treatments. Second experiment was SE (cotyledonary phage) desiccation. Factorial CRD used in two factors. First factor was poly-ethylene-glicol/PEG 8000 (0, 2.5, 5, 7.5 and 10%), while second factor was immersed periods (control, 3, 6, and 9 hours), in desiccant solution (base medium + PEG). Third experiment was studied of plantlet growth and development planlets. Based on CRD 2 factor method, the first factor was PEG concentrations from the second experiment. Second factor were active charcoal treatments (with or without), in basic media. The result showed that 2.5 mg L-1 ABA produced has highest mature somatic embryo (SE). Desiccation for 9 hours, induced the highestt germination frequencies (90.29%). The best growth of plantlets shown in previous experiments immersed desiccant PEG 2.5% for 9 hours, and cultured in basic media with 2 g L-1 of activated charcoal.Keywords: desiccant, embryogenic callus, maturation, PEG 8000, somatic embryo ABSTRAK Pemuliaan tanaman melalui teknik in vitro efektif bila metode regenerasi eksplan optimum telah diperoleh. Rendahnya frekuensi perkecambahan dan tingginya abnormalitas, menjadi kendala pada embriogenesis somatik jeruk. Penelitian terdiri atas 3 percobaan paralel, bertujuan mengoptimalkan metode embriogenesis somatik jeruk, khususnya Keprok Batu 55. Percobaan pertama pematangan kalus embriogenik menggunakan Rancangan Acak Lengkap (RAL) satu faktor, dengan perlakuan penambahan ZPT (kontrol, 3 mg L-1 BAP, dan 2.5 mg L-1 ABA) pada media dasar (MS modifikasi vitamin MW) diperkaya 500 mg L-1 ekstrak malt. Percobaan kedua desikasi embrio somatik (fase kotiledon) menggunakan RAL dua faktor. Faktor pertama konsentrasi poly-ethylene-glicol/PEG 8000 (0, 2.5, 5, 7.5 dan 10%), dan faktor kedua waktu perendaman (kontrol, 3, 6, dan 9 jam) pada larutan desikan (media dasar + PEG). Percobaan ketiga mempelajari pertumbuhan dan perkembangan planlet, menggunakan RAL dua faktor. Faktor pertama konsentrasi PEG planlet pada percobaan kedua, dan faktor kedua perbedaan media dasar (tanpa dan dengan arang aktif). Hasil percobaan menunjukkan penambahan 2.5 mg L-1 ABA menghasilkan maturasi embrio somatik terbaik. Desikasi 9 jam menghasilkan frekuensi perkecambahan 90.29%. Pertumbuhan terbaik ditunjukkan planlet yang pada percobaan sebelumnya direndam 9 jam desikan PEG 2.5%, dan dibesarkan pada media dasar dengan 2 g L-1 arang aktif.Kata kunci : desikan, embrio somatik, kalus embriogenik, PEG 8000, pematangan
EVALUASI KERAGAMAN GENETIK MUTAN HARAPAN GENERASI MV1 JERUK KEPROK SOE (CITRUS RETICULATA BLANCO) BERDASARKAN PENANDA MORFOLOGI DAN ISSR Husain, Indriati; Purwito, Agus; Husni, Ali; Mutaqin, Kikin H.; Susanto, Slamet
Jurnal Hortikultura Indonesia Vol. 7 No. 2 (2016): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.675 KB) | DOI: 10.29244/jhi.7.2.102-110

Abstract

ABSTRACTMandarin?s SoE is national variety originated from Mount of Mutis, Sub District of SoE, of Timur Tengah Selatan (TTS) District, East Nusa Tenggara (NTT). The genetic diversity of citrus can be induced by gamma ray irradiation on embryogenic callus cells thus producing new mutants. Genetic diversity detection can be based on morphological and ISSR markers. The aim of this research was to obtain information on the genetic diversity on putative mutants mandarin SoE induced by gamma ray irradiation based on morphology and markers ISSR. ISSR markers used are ISSR 1, 4, 6 and 8. Analysis of morphological diversity produced a dendrogram with the level of similarity between individuals each irradiation dose 83-95% with 5-17% genetic distance. Dendrogram analysis based on the genetic diversity ISSR markers showed high levels of 51-100% similarity and genetic distance 0-49%. Individuals samples obtained from gamma irradiation, based both morphological and ISSR markers, was different from individual's genetic make up before irradiation.Keywords: cluster, gamma ray, genetic distance, genetic diversitys, similarity ABSTRAKJeruk keprok SoE adalah jeruk varietas unggul nasional yang berasal dari Pegunungan Mutis, Kecamatan SoE, Kabupaten Timur Tengah Selatan (TTS), Provinsi Nusa Tenggara Timur (NTT). Keragaman genetik jeruk ini dapat diinduksi dengan iradiasi sinar gamma pada sel-sel kalus embriogenik untuk menghasilkan mutan yang solid. Deteksi keragaman genetik yang terbentuk dapat dilakukan secara morfologi maupun molekuler dengan marka ISSR. Tujuan Penelitian ini adalah untuk mendapatkan informasi mengenai keragaman genetik yang terjadi pada mutan harapan jeruk keprok SoE hasil iradiasi sinar gamma berdasarkan morfologi dan marka ISSR. Marka ISSR yang digunakan adalah ISSR 1, 4, 6 dan 8 pada beberapa mutan harapan jeruk keprok SoE. Analisis keragaman secara morfologi menghasilkan dendrogram dengan tingkat kemiripan antar individu masing-masing dosis iradiasi 83-95% dengan jarak genetik 5-17%. Dendrogram analisis keragaman genetik berdasar marka ISSR memperlihatkan tingkat kemiripan 51-100% dan jarak genetik 0-49%. Individu-individu sampel yang diuji hasil iradiasi gamma, baik secara morfologi dan marka ISSR, telah memiliki susunan genetik yang berbeda dari susunan genetik individu sebelum diiradiasi.Kata kunci: grup, jarak genetik, kemiripan, keragaman, sinar gamma
RADIOSENSITIVITAS DAN SELEKSI MUTAN PUTATIF JERUK KEPROK GARUT (CITRUS RETICULATA L.) BERDASARKAN PENANDA MORFOLOGI ,, Karyanti; Purwito, Agus; Husni, Ali
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 2 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.529 KB) | DOI: 10.24831/jai.v43i2.10417

Abstract

ABSTRACTMandarin Citrus cv. garut is a of local citrus variety with several superiority, but not competitive to imported citrus. Quality improvement have been the subject of citrus breeding programme. The objective of this research was to determine lethal dose 50 (LD50) and putative mutans regenerant selected based on morphological markers. Callus was irradiated at doses of 0, 20, 30, 40, 50, 60, 70, 80, 90 and 100 Gy and regenerated through somatic embryogenesis. The result of radiosensitivity showed that lethal dose 50 (LD50) analyzed by Curve Expert 1.4 software was 75.31 Gy. Observation on callus showed variation on morphology and weight of callus. Callus growth was not inhibited at doses 0-50 Gy but at doses 60-100 Gy callus growth was inhibited. Gamma irradiation also affected the formations of somatic embryos. After six weeks on maturation medium, the highest number of embryo somatic was produced from doses of 20 and 100 Gy and following culture on germination medium, the highest number of planlet was produced from doses of 20 and 40 Gy. Based on morphological characters, the selected regenerants had variability of 0-58%.Keywords: gamma irradiation, germination, lethal dosis 50 (LD50), maturation, somatic embryo
INDUKSI TETRAPLOID TUNAS PUCUK JERUK SIAM SIMADU (CITRUS NOBILIS LOUR) MENGGUNAKAN KOLKISIN SECARA IN VITRO Yulianti, Fitri; Purwito, Agus; Husni, Ali; Dinarti, Diny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 1 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (488.427 KB) | DOI: 10.24831/jai.v43i1.9593

Abstract

Seedless fruit is one of the criteria (necessary) to improve the quality of Simadu tangerine. The most effective method to obtain seedless triploid cultivars is hybridisation between tetraploid and diploid parents. Simadu tangerine is a diploid plant. Tetraploid Simadu tangerine can be obtained with doubling chromosome using colchicines.The aim of this research was to obtain tetraploid Simadu tangerine shoot which would serve as parent to produced seedless Simadu tangerine. Shoot-tips of Simadu tangerine without leaves were treated with colchicines at four different concentrations (0, 0.1, 0.2, and 0.3%) for 3 hours. The results showed that the high concentration of 0.3% reduced survival rate. The colchicine treatments reduced growth of shoot-tip of Simadu Tangerine.The leaves of colchicines treated shoots were thicker than control. Leaves from control (0% colchicine) and 0.1% colchicine treated shoots had 8.67 and 18.25 chloroplast per pair of guard cells. Compared to those of control, leaves with 0.1% colchicine had lower stomatal density, and larger stomatal size. It appeared that 0.1% colchicine treatment resulted in tetraploid Simadu Tangerine Shoot.Keywords:chloroplasts, doubling chromosomes, stomatal size, stomatal density
PENGARUH SUPLEMENTASI HIDROLISAT BULU AYAM DAN MINERAL ORGANIK TERHADAP KECERNAAN ZAT MAKANAN, PERTAMBAHAN BOBOT BADAN, DAN EFISIENSI RANSUM KAMBING PERANAKAN ETAWAH JANTAN Muhtarudin, Muhtarudin; Husni, Ali
Majalah Ilmiah Peternakan Vol 10 No 3 (2007)
Publisher : Fakultas Peternakan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRAK Tujuan penelitian ini adalah untuk mengevaluasi penggunaan suplementasi hidrolisat bulu ayam, mineral, makro organik (Ca, Mg organik) serta mikro organik (Zn, Cu, Cr, Se organik) guna meningkatkan kecernaan zat-zat makanan, pertambahan bobot badan, dan efisiensi ransum kambing peranakan Etawah jantan. Percobaan menggunakan kambing peranakan Etawah jantan sebanyak 20 ekor. Rancangan yang digunakan adalah rancangan acak kelompok dengan 4 perlakuan dan 5 ulangan. Perlakuan yang dicobakan adalah: R0 = 40% rumput gajah + 60% konsentrat, R1 = R0 + 3 % tepung bulu ayam, R2 = R1 + Mineral Makro-organik (Ca, Mg organik), R3 = R2 + Mineral Mikro-organik (Zn,Cu, Cr, Se organik). Hasil penelitian menyimpulkan bahwa perlakuan campuran bulu ayam, Ca-PUFA, Mg-PUFA (mineral makro organik) dan Zn, Cu, Se, dan Cr, lisinat (mineral mikro organik) atau R3 dapat meningkatkan kecernaan bahan organik, energi, pertambahan bobot badan, dan efisiensi ransum dari kambing peranakan Etawah jantan. THE EFFECT SUPLEMENTATION OF HYDROLYZED OF FEATHER MEAL AND ORGANIC MINERAL ON NUTRIENTS DIGESTIBILITY, DAILY GAIN, AND RATIONS EFFICIENCY OF MALE ETAWAH CROSS GOATS ABSTRACT The objective of research were to evaluate the effect of supplementation of hydrolyzed of feather meal, organic macro mineral (Ca and Mg-Polyunsaturated Fatty Acid /PUFA) and organic micro mineral (Zn, Cu, Cr, and Se-lysinat) to improve nutrient digestibility, daily gain and rations efficiency of male Etawah cross goats. The research were used 20 male Etawah cross goats. Random block design were used this research was used, with 4 treatments and 5 replications. The treatments were arranged : R0= 40 % of forage + 60% concentrates; R1= R0 + 3% of hydrolyzed of feather meal ; R2= R1 + organic macro mineral (Ca and Mg -PUFA); R3 = R2+ organic micro mineral (Zn,Cu, Cr, and Se-lysinat). The results showed that mixing treatment of hydrolyzed feathers meal, organic macro and micro mineral (Ca and Mg-Polyunsaturated Fatty Acid /PUFA and Zn, Cu, Cr, and Se-lysinat)/R3 had significantly effect of increasing organic digestibility, digestible energy, daily gain and rations efficiency of male Etawah cross goats.
EMBRIOGENESIS SOMATIK JERUK KEPROK (CITRUS RETICULATA L. CV BATU 55) ASAL HASIL PERLAKUAN KOLKISIN Purwito, Agus; Prayogi, Mohamad; Kosmiatin, Mia; Husni, Ali
Jurnal Hortikultura Indonesia Vol. 6 No. 3 (2015): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (315.475 KB) | DOI: 10.29244/jhi.6.3.161-171

Abstract

ABSTRACTThe objective of this study was to obtain the best method of regeneration through somatic embryogenesis of citrus cv Batu 55 from callus resulted from in vitro polyploidization by colchicine. The experiment was conducted at the Laboratory of ICABIOGRAD, Bogor and Tissue Culture Laboratory, Department of Agronomy and Horticulture, Bogor Agricultural University from March 2014 until September 2014. This study consisted of proliferation of embryogenic callus, maturation, germination of somatic embryos, growth of shoots and roots. The research were comprised of four experiments, namely: 1). The effect of Phytagel concentration (2.5, 3.0, 3.5 and 4.0 g L-1) on proliferation of embryogenic callus, with 3 replications. Each experimental unit consisted of 3 clumps of callus, 2). The effect of ABA concentration (0, 0.1, 0.3 and 0.5 mg L-1) on somatic embryo maturation with 6 replications. Each experimental unit was one culture vessel containing five somatic embryos at globular phase, 3) The effect of vitamin composition (vitamin MS and vitamin MW) on germination of somatic embryo with 16 replications. Each experimental unit was one culture vessel containing four somatic embryos at cotyledonary phase, and 4) The effect 0.5 mg L-1 of plant growth regulators (NAA, IAA, IBA) and vitamin (MS and MW) on rooting and shoot elongation of germinated somatic embryos. Experiment was repeated five times. Each experimental unit was one culture vessel containing one plantlet as explant. All experiments were arranged as a completely randomized design. The result showed that the best concentration of Phytagel for callus proliferation was 2.5 g L-1. Maturation of somatic embryos was better when the somatic embryos were planted on medium supplemented with ABA 0.5 mg L-1. The MS medium supplemented with vitamin MS was better than supplemented with vitamin MW for the formation of plantlets, while roots and shoots elongation of the plantlet was better when explant was planted on the MS medium supplemented with vitamins MS and IBA 0.5 mg L-1.Key words: proliferation, maturation, germination, embryogenic callus, plantlet.ABSTRAKPenelitian ini bertujuan untuk mendapatkan metode embriogenesis somatik terbaik dari kalus Jeruk Keprok cv Batu 55 yang mendapatkan perlakuan poliploidisasi dengan kolkisin. Penelitian dilakukan di Laboratorium Balai Besar Litbang Bioteknologi & Sumber Daya Genetik Pertanian, dan Laboratorium Kultur Jaringan, Departemen Agronomi dan Hortikultura, Institut Pertanian Bogor mulai bulan Maret 2014 hingga September 2014. Penelitian ini terdiri atas proliferasi kalus embriogenik, pendewasaan, perkecambahan embrio somatik (ES), pertumbuhan tunas dan akar. Penelitian terdiri atas empat percobaan, yaitu: 1). Pengaruh konsentrasi Phytagel (2.5, 3.0, 3.5 dan 4.0 g L-1) terhadap proliferasi kalus embriogenik, dengan 3 ulangan, dimana setiap satuan percobaan terdiri atas 3 klum kalus, 2). Pengaruh konsentrasi ABA (0, 0.1, 0.3 dan 0.5 mg L-1) terhadap pendewasaan ES dengan 6 ulangan. Setiap satuan percobaan ialah satu botol kultur yang ditanam lima ES fase globular, 3) Pengaruh komposisi vitamin (vitamin MS dan vitamin MW) terhadap perkecambahan ES dengan 16 ulangan. Setiap satuan percobaan ialah satu botol kultur yang ditanami empat ES fase kotiledon, dan 4) Pengaruh 0.5 mg L-1 zat pengatur tumbuh (NAA, IAA, IBA) dan vitamin (MS dan MW) terhadap pertumbuhan tunas dan akar pada ES yang telah berkecambah. Setiap perlakuan diulang lima kali. Setiap satuan percobaan ialah satu botol kultur yang berisi satu planlet. Seluruh percobaan disusun menggunakan rancangan acak lengkap (RAL). Hasil menunjukkan bahwa konsentrasi terbaik Phytagel untuk proliferasi kalus adalah 2.5 g L-1. Pendewasan menjadi ES fase kotiledon akan lebih baik jika ES ditanam pada medium dengan ABA 0.5 mg L-1. Untuk pembentukan planlet, ES fase kotiledon akan lebih baik ditanam dalam medium MS yang ditambah vitamin MS dibanding yang ditanam pada medium MS ditambah vitamin MW. Medium untuk pertumbuhan tunas dan akar terbaik adalah medium MS yang ditambah dengan vitamin MS dan IBA 0.5 mg L-1.Kata kunci: proliferasi, pendewasaan, perkecambahan, kalus embriogenik, planlet.
Protoplast Fusion between Indonesian Citrus maxima (Burm.) Merr. and Citrus reticulata L.: A Preliminary Report Wulandari, Dyah Retno; Purwito, Agus; Susanto, Slamet; Husni, Ali; Ermayanti, Tri Muji
AGRIVITA, Journal of Agricultural Science Vol 40, No 2 (2018): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i0.950

Abstract

Protoplast fusion is a useful technique for citrus genetic improvement. Establishment of protoplast technology could produce triploid seedles citrus and new citrus genetic combination. The aim of this research was to establish protoplast isolation, protoplast fusion between pummelo ?Nambangan? (Citrus maxima) and keprok ?Garut? (C. reticulata), and its protoplast culture. Protoplasts were isolated from leaves of in vitro seedlings of pummelo ?Nambangan? and embryogenic callus of keprok ?Garut? with two different compositions of enzymes. The results showed that optimum enzymes compositions for cells mesophyl wall degradation were consisted of 0.5 % Onozuka cellulase RS10, 0.5 % Macerozyme R10 and 0.1 % Pectolyase Y23. Optimum enzymes composition for callus embryogenic cells wall degradation were 0.5 % Onozuka cellulase RS10 added with 0.5 % Macerozyme R10. Protoplast fusion was conducted with PEG-mediated. Protoplast mixture were incubated in 40 % PEG for 8-10 min and after that could be observed 40.6 % of unfused protoplasts, 4.6 % of homofused mesophyl protoplasts, 28.2 % of homofused callus protoplasts, 7.8 % of heterofused and 9.6 % of multifused protoplasts from mesophyl and callus. Cells divided at the first week and began to form cell colonies afterwards in liquid culture.
PENERBITAN BANK GARANSI PADA LEMBAGA PERBANKAN MENURUT UNDANG – UNDANG NOMOR 10 TAHUN 1998 Husni, Ali
DEDIKASI JURNAL MAHASISWA Vol 2, No 1 (2014)
Publisher : DEDIKASI JURNAL MAHASISWA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (122.88 KB)

Abstract

AbstractProvision of Bank Guarantee is one of the functions of the Bank, in addition to the function of providing a wide range of banking services. As a policy, the Bank Guarantee given to customers of the Bank with the aim of providing assistance that are pertinent customer support who will perform work tasks that are very limited and selected. Bank to Bank Guarantee issuing a written acknowledgment that it agreed to adhere to the insured in order to fulfill the obligation secured within a certain period of time and with certain requirements in the form of payment of a certain sum of money in the future is assured if it did not fulfill its obligations to the recipient warranty or guarantee in the future if it turns default (default). Provision of Bank Guarantee is one of the functions of the Bank, in addition to the function of providing a wide range of banking services. As a policy, the Bank Guarantee given to customers of the Bank with the aim of providing assistance that are pertinent customer support who will perform work tasks that are very limited and selected.
UKURAN-UKURAN TUBUH SAPI KRUI JANTAN DAN BETINA DI KABUPATEN PESISIR BARAT LAMPUNG Hamdani, Muhammad Dima Iqbal; Adhianto, Kusuma; -, Sulastri; Husni, Ali; -, Renitasari
Jurnal Ilmu Ternak Universitas Padjadjaran Vol 17, No 2 (2017)
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jit.v17i2.16068

Abstract

AbstrakPenelitian ini bertujuan untuk mengetahui ukuran-ukuran tubuh sapi Krui jantan dan betina di Kabupaten Pesisir Barat provinsi Lampung.  Penelitian ini dilakukan pada November-Desember 2016.  Sampel yang diamati sebanyak 55 ekor sapi jantan dan 71 ekor sapi betina.  Metode survey digunakan dalam penelitian ini, dan sampel tersebut ditentukan dengan menggunakan purposive sampling.  Data dianalisis dengan uji t.  Peubah yang diamati meliputi bobot badan, lingkar dada, panjang badan, dan tinggi pundak.  Hasil penelitian menunjukan bahwa rata-rata bobot badan sapi Krui jantan dan betina poel 0 (125,4±117 kg), poel 1 (148,6±136,2 kg), poel 2 (189,6±176,1 kg), poel 3 (234,5±208,4 kg), lingkar dada poel 0 (118±110 cm), poel 1 (132,9±128,3 cm), poel 2 (134,5±129,5 cm), poel 3 (137±130,3 cm), panjang badan poel 0 (103±97,2 cm), poel 1 (110,3±104,1 cm), poel 2 (124,6±109,8 cm) poel 3 (133,6±127,4 cm), sedangkan rata-rata tinggi pundak poel 0 (91,3±86,7 cm), poel 1 (102,5±97,8 cm), poel 2 (105,1±101,1 cm), poel 3 (113±104,7 cm).  Hasil penelitian ini menunjukkan bahwa bobot badan, lingkar dada, panjang badan, dan tinggi pundak sapi Krui jantan dan betina pada kelompok umur yang sama menunjukkan perbedaan yang nyata (P<0,05).  Kata Kunci : Sapi Krui, Sifat kuantitatif, Bobot badan, Lingkar dada, Panjang badan, Tinggi pundak.
Perkecambahan dan Perbanyakan Gaharu secara In Vitro Kosmiatin, Mia; Husni, Ali; Mariska, Ika
Jurnal AgroBiogen Vol 1, No 2 (2005): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Agarwood (Aquilaria malaccensis Lank) is one of the forest wood that are continously exploited. Currently, the Indonesian export of agarwood is decreasing because its population is endangered by excessive logging. Agarwood propagations need technology for reproduction of agarwood seedlings and their fungal inoculum. In vitro technique for germination of recalsitrant seeds and micropropagation are technologies that can be used for propagation of agarwood seedlings. An experiment was done to develop techniques for in vitro germination and micropropagation of agarwood. The in vitro germination was done using two different techniques. Firstly, sterile seeds were germinated on an MS medium + 50 mg/l PVP, 50 mg/l GA, and 1 mg/l BA or kinetin. Secondly, sterile seeds were germinated on basal medium of MS, 1/2 MS medium, MS medium without vitamins, as well as on MS medium without pyridoxine, nicotinic acid and WPM. Shoot initiations and multiplications were done on MS and 1/2 MS media containing 1, 3, or 5 mg/l BA. The explants used were cotyledone nodes, terminal shoots, single node with leaf, and sinle node without leaf. The results showed that the seed germination rate on the different media ranged from 7,14 to 50%. The seed germination rate on the MS medium without vitamis was the highest. The best explants for shoot induction and multiplication was single node with leaf which was cultured on MS + 1 mg/l BA.