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PEMANFAATAN LIMBAH KRUSTASEA DALAM PEMBUATAN GLUKOSAMIN HIDROKLORIDA DENGAN METODE AUTOKLAF Suptija, Pipih; Ibrahim, Bustami; Ernawati, Ernawati
Jurnal Teknologi Perikanan dan Kelautan Vol 5 No 2 (2014): NOVEMBER 2014
Publisher : Fakultas Perikanan dan Ilmu Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3441.113 KB) | DOI: 10.24319/jtpk.5.171-179

Abstract

Utilization of crustacean shell waste to produce chitosan can continue by derivatisation to produced glucosamine. GlcN HCl is one of the nutraceuticals product widely consumed for reducing osteoarthritis symptoms. The minimum local production of glucosamine itself encourages a proper glucosamine production technology. The purpose of this study was to obtain optimum production technique of glucosamine hydrochloride. Production of glucosamine hydrochloride used presured hydrolysis method by pressured treatment (autoclaving), heating time (hours), and acid (% v/v). Characteristic of glucosamine hydrochloride was determined for solubility parameters, appearance, yields, value loss on drying (LOD), the melting point temperature and FTIR absorption. The quality standard of hydrolyzed glucosamine refers to the standard of United State Pharmacopeia. The results showed that the optimum techniques of glucosamine hydrochloride production were using HCl (8%) (v/v) and 1 atm pressure for 1 hour. Hydrolyzedglucosamine hydrochloride was completely dissolved in water, yellowish-white powder appearance with (69.80%) of yield, LOD value was 0.92%, and the melting point temperature was 190-193 °C. FTIR spectrum showed functional group of OH at 300-3263 cm-1 and amida group at 1566 cm-1, wassimilar absorption band patterns with hydrolyzed glucosamine hydrochloride of standard.
OPTIMASI DAN PEMODELAN PROSES RECOVER FLAVOR DARI LIMBAH CAIR INDUSTRI PENGOLAHAN RAJUNGAN DENGAN REVERSE OSMOSIS Uju, .; Ibrahim, Bustami; Trilaksani, Wini; Nurhayati, Tati
Jurnal Ilmu Pertanian Indonesia Vol. 14 No. 1 (2009): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The waste water of blue crab pasteurization has potential in environmental pollution. It contained TSS of 206.5mg.1-1, BOD 7,092.6mg.1-1 and COD of 51,000mg.1-1. on the other hand, it also contains an interesting flavor compound, which composed of 0.23% non protein nitrogen and 17 amino acids where the highest was glutamic acid one. In this study, pre-filtration step using filter size 0.3 µ followed by reverse osmosis has been used to reduce these pollutions load and flavor compound recovery. During pre-filtration steps, TSS was reduced to 74.8% so turbidity decrased reased until 31%. After reverse osmosis process, BOD, and COD decreased more than 99%, and there was no amino acids detected in permeate stream. Factors that affect performance of reverse osmosis were transmembrane pressure, temperature and pH. The higher transmembrane pressure, temperature and pH resulted the higher the flux permeate. The use of higher temperature make flux increasing, eventually increasing transmembrane pressure make the flux increased only at transmembrane pressure less than 716 kPa. The protein rejection was influenced unsignifanctly by transmembrane pressure, temperature and pH. During concentrating flux declined exponentially by time function. At concentration factor 2.75 resulted 79% and 12% of increasing protein and NPN, respectively. The amino acids content can be increased 2?23 times of the origin. Even arginin and sistin, the amino acids that were undetectable initially, but they can bedetected at concentration of 0.0360 and 0.0250 (w/v) respectively at the end of the process. Hidrolysis and fermentation process can increase the amino acid content 31?45 times.
PRODUKSI BIODIESEL DARI BIOMASSA Chlamydomonas sp. ICBB 9113 DIKULTIVASI MENGGUNAKAN MEDIA YANG MURAH: EFEKTIFITAS DARI BEBERAPA METODE EKSTRAKSI Patmawati, Patmawati; Ibrahim, Bustami; Setyaningsih, Iriani; Sudadi, Untung
Widyariset Vol 17, No 2 (2014): Widyariset
Publisher : LIPI-Press

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Abstract

The main challenges to overcome in biodiesel production from microalgae are lower oil yield, as compared to those derived from plant and animal biomass, and expensive culture media. This work was aimed to compare the effectivity of three extraction methods differed in solvent used, e.g. n-hexane (N-hex), ethanol (Eth), and mixture of chloroform-methanol-water (CMW), to extract crude lipid and biodiesel from dry biomass of Chlamydomonas sp. ICBB 9113. This microalgae was cultivated in a cheap culture media using N and P soil fertilizers as nutrient sources. The results showed that, by using N-hex, Eth, and CMW methods, it could be extracted, respectively, 0.06%, 4.51%, and 20.45% crude lipid, and 384.2, 1333.8, and 2430.6 mg/100g biodiesel. The fatty acid profile of the studied microalgae biomass was: C8:0 (0.11%), C10:0 (0.09%), C14:0 (7.70%), 16:0 (1.39%), C18:0 (0.85%), C14:1 (5.12%), C16:1 (7.09%), C18:1 (8.28%), C18:2 (12.80%), and C18:3 (42.57%). Fatty acid characterization showed that Chlamydomonas sp. ICBB 9113 was dominated by C18: 3 and C16:0. Therefore, these microlagae were suitable to be used as raw material for biodiesel production to substitute the conventional fuel.
BIOFILM DENSITY ON THE ELECTRODE IS POSITIVELY CORRELATED WITH THE BIOELECTRICITY OF THE MICROBIAL FUEL CELL OF FISHERIES WASTEWATER Ibrahim, Bustami; Uju, Uju; Mukti, Alvindo Chrisna
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 22 No. 1 (2019): Jurnal Pengolahan Hasil Perikanan
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.376 KB) | DOI: 10.17844/jphpi.v22i1.25880

Abstract

Microbial fuel cell (MFC) is a bioreactor utilizing bacteria as electrocatalysts to convert bioenergy from biomass into electrical energy. The aim of this research were to determine the effects of the electrode distance on the bacterial density and the electrical value generated by the MFC as well as to evaluate the ability of MFC in reducing the pollutant. Single chamber MFC system with various electrode distances including 2 cm, 4 cm, and 6 cm were assembled. The wastewater of fish pindang processing was used as the mediumfor the MFC. The results showed that the distance had no effect on the biofilm density of the electrode and the reduction of the wastewater pollutant load. However, the distance affected the electrical value of theMFC. Biofilm density on the MFC electrode after 120 hours was 0.65-6.46 CFU/ cm2. The highest voltage was obtained from the 6 cm electrode distance with the voltage 0.38±0.01 V. Positive correlation (R2 = 0.99)between microbial density and electricity produced at the cathode was observed, but weak at the anoda (R2 = 0.47). The MFC system could decrease the BOD value up to 50.78% and COD up to 33.29%, however the TAN value was increased to 6 mg/L.
HYDROXYAPATITE PRODUCTION FROM CUTTLEBONE AS BONE SCAFFOLD MATERIAL PREPARATIONS Henggu, Krisman Umbu; Ibrahim, Bustami; Suptijah, Pipih
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 22 No. 1 (2019): Jurnal Pengolahan Hasil Perikanan
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2244.688 KB) | DOI: 10.17844/jphpi.v22i1.25869

Abstract

The increasing production of cuttlefish has been associated with the increasing of by-product waste particularly cuttlebone. Cuttlebone is known to contain an inorganic element in form of calcium carbonate(CaCO3) which can be utilized as a source of calcium oxide (CaO) for hydroxyapatite synthesis. This study was aimed to determine the physicochemical characteristics of the cuttlebone and the optimum calcination temperature for CaO extraction and hydroxyapatite synthesis. This study was divided into three steps. Firstly, analysis of the cuttlebone physicochemical properties; secondly, extraction and characterization of the CaO with different calcination temperature (500°C, 600°C, 700°C for 6 hours); and thirdly, hydroxyapatite synthesis using a combination of hydrothermal method at 200°C 6 hours and different calcination treatments (800°C, 900°C, 1,000°C for 1 hour). The results showed that the cuttlebone contained moisture 3.54±0.11%,lipid 0.32±0.19%, protein 4.78±0.23%, carbohydrate 5.29±0.02%, and ash 89.61±0.26. The main element of the ash was CaCO3 aragonite characterized by the high absorption at wavelengths of 1,795; 1,507;1,083; 871; 713 and 700 cm-1. The calcination treatment of 700°C produced the highest amount of CaO. The hydroxyapatite produced with a combination of hydrothermal and calcination temperature 1,000°Chad calcium phosphate ratio (Ca/P) 1.66, crystalline level 90.10%, amorphous level 9.90% and particles morphology of rod-shaped.
CHARACTERIZATION OF TUNA JERKY WITH THE ADDITION OF SEAWEED (EUCHEUMA COTTONII) FLOUR Lobo, Reni; Santoso, Joko; Ibrahim, Bustami
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 22 No. 2 (2019): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (750.311 KB) | DOI: 10.17844/jphpi.v22i2.27678

Abstract

Fish jerky has hard texture which could make the appearance became less attractive to consumers. The aim of this study was to characterize tuna jerky with the addition of seaweed flour E. cottonii in order to increase acceptability by consumers. Result shown that seaweed flour characterization was consisted dietary fiber 73.95±0.45%, viscosity 107.02±0.51 cPs, gel strength 435.03±4.99 g/cm2, carageenan 54.56±0.18%, heavy metal Hg was <0.002 ppm, Pb was <0.004 ppm and Cd was 0.063±0.001 ppm, water content 12.63±0.08% and yield 6.07±0.08%. Based on research result, the additional of 2.5% E. cottonii concentration flour resulting high quality jerky with appearance value was 7.60, flavour was 7.67, texture was 5.7 and taste was 7.63 from 1-9 scales. Result from paired comparison test against commercial jerky (beef) resulting positive value, that means the quality of tuna jerky had better quality compared to commercial jerky and well accepted by panelist. Proximate analysis of tuna jerky shown that protein content 30.24±0.1%, water 11.32±0.02%, lipid 3.03±0.00% and ash 5.69±0.06%. Texture analysis results shown hardness 827.50±15.67, adhesiveness 0.09±0.02 and fracture 10.95±2.24.
CHITOSAN IS A NATURAL MATERIAL EXTRACTED FROM DEACETYLATED CHITIN OF CRUSTACEAN SHELL USED FOR MANY KINDS OF FUNCTIONS, NOT ONLY FOR FOOD BUT ALSO NON FOOD PRODUCT. THE RESEARCH OBJECTIVE IS TO FIND OUT THE EFFECT OF CHITOSAN USED FOR FILLER WITHIN TRANSPARENCY SOAP TO THE CHARACTERISTIC OF PHYSICAL, CHEMICAL AND BIOEFFECT OF END PRODUCT, AND TO FIND OUT WHICH CONCENTRATE OF CHITOSAN WILL BE USED FOR THE BEST PRODUCT. THE RESEARCH WAS DIVIDED BY TWO STEPS, PRE-RESEARCH AND MAIN RESEARCH.  PRE-R Ibrahim, Bustami; Suptijah, Pipih; Amin, Hijrah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 13 No. 1 (2010): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v13i1.3140

Abstract

Chitosan is a natural material extracted from deacetylated chitin of crustacean shell used for many kinds of functions, not only for food but also non food product. The research objective is to find out the effect of chitosan used for filler within transparency soap to the characteristic of physical, chemical and bioeffect of end product, and to find out which concentrate of chitosan will be used for the best product. The research was divided by two steps, pre-research and main research.  Pre-research resulted that transparency soap with 5% chitosan was the best choice based on organoleptic test which was the best in toughness, and based on chemical also microbiological analyses. The soap with 5% chitosan has TPC (Total Plate Count) amount to  2.0x101, while the one without chitosan contained TPC 6.5x101. The main research showed that transparency soap with 5% chitosan has better quality compare to commercialized one.
PENGARUH RASIO COD/NO3 PADA PARAMETER BIOKINETIKA PROSES DENITRIFIKASI LIMBAH CAIR INDUSTRI PERIKANAN DENGAN LUMPUR AKTIF IBRAHIM, BUSTAMI; ERUNGAN, ANNA C.; SARI, NONI DWI
Akuatik: Jurnal Sumberdaya Perairan Vol 3 No 1 (2009): AKUATIK : Jurnal Sumberdaya Perairan
Publisher : Program Studi Perikanan, Fakultas Pertanian, Perikanan dan Biologi Universitas Bangka Belitung

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Abstract

Indonesia’s Fishery Industries has been developed very rapidly. This can cause negative effect such as increase in wastewater that involved highly organic substrates that will danger the environment. This effluent must be treated before flow it to environment and usually treated by biological wastewater treatment through nitrification and denitrification process simultaneously using activated sludge. This study doing denitrification process using activated sludge with KNO3 added, the treatment using different COD/NO3 ratios which are 3,04, 4,24 and 6,8, and monitoring do in HydraulicRetention Time (HRT) 4,5; 3,5; 2,5; 2,0; 1,5; 1,0; 0,75; 0,5 and 0,25 day. Results shows for increasingly MLVSS, Efficiency decreasingly COD, Efficiency decreasingly NO3 and denitrification rate for ratio COD/NO3 3,04 are 52,02%, 32,25%, 16,25% and 0,000759 mgNO3/mgVSS.day and for ratio COD/NO3 4,24 are 30,6%, 24,46%, 41,80% and 0,001 mgNO3/mgVSS.day also for ratio COD/NO3 6,8 are 44,7%, 23,88%, 15,95% dan 0,01 mgNO3/mgVSS.day. This efficiency result use for determine the biokinetic parameter. Biokinetic parameter such asa Y (yield), Ks and Knh (Monod Constant saturutated), Kd (endogenous decay) also µm results for rasio COD/NO3 3,04 are 0,212mgVSS/mgCOD, 6,76 mg/lCOD, 1,54 mg/lNO3, 0,105 day-1, dan 0,29 day-1 for rasio COD/NO3 4,24 are 0,267mgVSS/mgCOD, 16,77 mg/lCOD, 1,44 mg/lNO3, 0,024 day-1, dan 0,95 day-1 also for rasio COD/NO3 6,8 have results 0,2495mgVSS/mgCOD, 24,37 mg/lCOD, 1,58 mg/lNO3, 0,177 day-1, dan 0,89 day-1. This biokinetic parameter can be used to improved the wastewater treatment plant.
PERANCANGAN GEARBOX TRAKTOR TANGAN 2 KECEPATAN 1 MUNDUR DENGAN SISTEM PEMINDAH GIGI SYNCHROMESH Ibrahim, Bustami; Fadhila, Aziza Noor; Romansyah, Rofan Yulian
Machine : Jurnal Teknik Mesin Vol 4 No 1 (2018): Journal Machine Vol. 4 No. 1 Januari 2018
Publisher : Jurusan Teknik Mesin Fakultas Teknik Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (744.225 KB) | DOI: 10.33019/jm.v4i1.447

Abstract

Abstrak                                                                                                                                Saat ini Polman Bandung sedang melakukan pengembangan hand tractor atau traktor tangan melalui Pusat Pengembangan Power Train Otomotif (P3TO) dengan fokus kajian yang penting untuk dilakukan saat ini adalah perancangan gearbox baru untuk traktor tangan yang akan dikembangkan. Pengembangan-pengembangan yang akan dilakukan saat ini ialah penambahan kecepatan pada gearbox, peningkatan perfoma gearbox dan peningkatan kemudahan operasi dari gearbox yang dilihat dari segi sistem pemindah gigi yang ada pada gearbox. Hal ini didasarkan pada model-model gearbox pada traktor tangan yang ada di Indonesia, dinilai memiliki kekurangan pada aspek-aspek yang akan dikembangkan saat ini terutama pada jumlah kecepatan dan  sistem pemindah giginya, maka dari itu pada tugas akhir ini dipilih multi speed gearbox dengan 2 kecepatan 1 mundur dengan sistem pemindah gigi synchromesh untuk mengatasi kekurangan-kekurangan gearbox traktor tangan yang ada saat ini. Tahapan perancangan dilakukan berdasarkan metode perancangan VDI 2222. Penelitian ini bertujuan untuk menghasilkan rancangan gearbox yang sesuai dengan daftar tuntutan dengan sistem dan performa yang lebih baik dari yang ada saat ini di pasaran.
KARAKTERISTIK GLIKOGEN TEMILOK (Bactronophorus thoracites) BERMUTU RENDAH SEBAGAI KO-PRESIPITAN ASAM DEOKSIRIBONUKLEAT SYAPUTRA, DENNY; IBRAHIM, BUSTAMI; WIDODO, PUTUT TJAHYO
Akuatik: Jurnal Sumberdaya Perairan Vol 6 No 2 (2012): AKUATIK : Jurnal Sumberdaya Perairan
Publisher : Program Studi Perikanan, Fakultas Pertanian, Perikanan dan Biologi Universitas Bangka Belitung

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Abstract

Temilok (Bactronophorus thoracites) is one of Teredinidae or family of wood-borer shellfish in fact have a fairly high polysaccharide content, particularly interesting source of glycogen. The object of this experiment is to investigate the impurity contents (nitrogenous compounds and nucleic acids) of extracted glycogen from low quality of temilok based on Nicoletti and Baiocchi glycogen polysaccharide extraction patent method by comprising boiling the whole tissue in an aqueous solution of 40% potassium hydroxide (KOH) and treated with 12% cationic resin Amberlite IR-120 carried out for 16 hours under stirring at room temperature, and the ability of the extracted glycogen as co-precipitant in the precipitation of deoxyribonucleic acid (DNA) of human femur.  Nitrogen and nucleic acid content of extracted glycogen were spectrophotometrically measured at 260 nm, 280 nm and 320 nm. Extracted glycogen samples was characterized by determining the glucose content  quantitatively using phenol-sulphate method which measured spectrophotometrically at 490 nm .  The nitrogen content of extracted glycogen was about 0,8%, yield about 6,5% glycogen characterized as the lowest nucleic acids content i.e 0,07 mg/mL.  In order to asses the ability of the extracted glycogen as carrier or co-precipitant of low copy number DNA, we added 20 µL the 2% solution of the extracted glycogen into first step of femur DNA procedure of precipitation.  Results revealed that by adding the extracted glycogen solutions, the yield of DNA were not significantly affected at the level of 0,05 though the yield of DNA decreased against blanko about 0,005 ng/µL of 0,0401 ng/µL