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SENYAWA ASAM 2- METILESTER-1-H-PIROL-4-KARBOKSILAT DALAM EKSTRAK ETIL ASETAT BUAH SALAK VARIETAS BONGKOK SEBAGAI ANTIOKSIDAN DAN ANTIHYPERURICEMIA [STUDIES ON 2-METHYLESTER-1-H-PYROLLE-4-CARBOXYLIC ACID COMPOUND IN ETHYLACETATE EXTRACT OF SNAKE FRUIT VAR Afrianti, Leni Herliani; Sukandar, Elin Yulinah; Ibrahim, Slamet; Adnyana, I Ketut
Jurnal Teknologi dan Industri Pangan Vol. 21 No. 1 (2010): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (371.817 KB)

Abstract

The aim of the study was to determine the antioxidant and antihyperuricemia activity of ethyl acetate extract of snake fruit (Salacca edulis Reinw.) var. Bongkok. The research methods used in this study comprised  of three stages. First stage, the isolation processes, consist ed of maceration, fractionation, and purification using several techniques of chromatography. The chemical structures of the isolated compounds were determined based on UV, IR, 1-D NMR, and 2-D NMR spectral data. The ethyl acetate extract of snake fruit var. Bongkok isolated was a new compound 2-methylester-1-H-pyrolle-4- carboxylic acid. In the second stage the antioxidant activity of the extract and the isolated compounds were measured by 1,1 diphenol  (DPPH) method. The antioxidant activity of the extracts and the isolated compounds were expressed as IC50, The ethyl acetate extracts at concentrations of 0.2, 2, 20, 200, 400, and 2000 µg/mL showed inhibition of 9.67, 4.47, 41.89, 96.06, 82.54, and 90.60 % respectively, with an IC50 of 1.6 µg/mL. Ascorbic acid standards at the same concentration range showed an  IC50 of 0.54 µg/mL. Meanwhile, at the same concentrations the 2-methylester-1-H-pyrolle-4-carboxylic acid showed free radical inhibition of 17.48, 21.48, 18.14, 31.87, and 62.34 % respectively, with an  IC50 of 3.27  µg/mL. During the third stage,  the antihyperuricemic properties of the extracts and the isolated compound were examinated in vitro using inhibition of xanthin oxidase method. The ethyl acetate extracts at concentrations of 0.01, 0.02, 0.2, 2, and 2000  µg/mL showed xanthin oxidase inhibition of 49.24, 49.58, 50.28 and 52.26 % respectively, with an  IC50 of 24.75 µg/mL. At the same concentrations, the 2-methylester-1-H-pyrolle-4- carboxylic acid, showed xanthin oxidase inhibition of 27.7, 30.5, 37.3, 50.27 and 50.55 % respectively, with an IC50 of 48.86 µg/mL.  Allopurinol as a standard drug showed an IC50 of 0.92 µg/mL.
SYNTHESIS AND CHARACTERIZATION MOLECULARLY IMPRINTED POLYMERS FOR ANALYSIS OF DIMETHYLAMYLAMINE USING ACRYLAMIDE AS MONOMER FUNCTIONAL Amin, Saeful; Damayanti, Sophi; Ibrahim, Slamet
Jurnal Kefarmasian Indonesia VOLUME 8, NOMOR 2, AGUSTUS 2018
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (334.25 KB) | DOI: 10.22435/jki.v8i2.330

Abstract

A selective separation techniques with Molecularly Imprinted Polymer (MIP) for High-Performance Liquid Chromatography (HPLC) has been developed for the assay of Dimethylamylamine (DMAA) doping compounds. Molecular imprinted polymer (MIP) is a technique to produce a polymer having the cavity due to the disposal of the templates, in which the cavity serves to recognize the molecules of the same size, structure, chemical and physical properties. The selectivity and affinity of the templates itself will increase, while the concentration value is increasing. MIP is made by DMAA as template, acrylamide as functional monomer, ethylene glycol dimethacrylate (EGDMA) as cross linking, azobisisobutyronitrile (AIBN) as the initiator and chloroform as a porogen solvent; using bulk method. The aim of research are conduct the MIP for the DMAA compound analysis, then the formed MIP is characterized by using Fourier Transform Infra Red (FTIR) and Scanning Electron Microscopy (SEM) to find out the polymer complexes formed and the morphological form of the MIP. The MIP formed then was analyzed by using High-Performance Liquid Chromatography (HPLC) to know the amount of the DMAA, the adsorption capacity, and the adsorption condition found in the MIP. The result of analysis on the content of DMAA in the MIP by using UV-Vis Spectrophotometer is 1.957 mg. Scanning Electron Microscopy (SEM) shows that the MIP has irregular and rough morphological structure; while the NIP has irregular morphology structures and smooth surfaces shape
SIMULTANEOUS DETERMINATION OF PARACETAMOL AND IBUPROFENE MIXTURES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY Damayanti, Sophi; Ibrahim, Slamet; Firman, Kurnia; Tjahjono, Daryono H
Indonesian Journal of Chemistry Vol 3, No 1 (2003)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (73.538 KB) | DOI: 10.22146/ijc.21899

Abstract

Analytical method for the determination of paracetamol and ibuprofene mixtures has been developed by High Performance Liquid Chromatography using C-18 column and acetinitrile - phosphate buffer pH = 4.5 (75:25) containing 0.075% sodium hexanesulfunate as a mobile phase. The detector was set at 215 nm. Using such conditions, retention time for paracetamol and ibuprofen was 4.89 and 7.11 min, respectively. The recovery for paracetamol and ibuprofen was found to be 101.07± 0.73% and 102.02 ± 1.58%, respectively. The detector limits of the method was 1.30 and 1.60 ?g/mL with the relative standard deviation (RSD) 0.74 and 1.52% for paracetamol and ibuprofen, respectively.
Identification of physical interaction between levodopa–benzerazide hydrochloride Nugrahani, Ilma; Asyarie, Sukmadjaja; Soewandhi, Sundani Noerono; Ibrahim, Slamet
INDONESIAN JOURNAL OF PHARMACY Vol 18 No 2, 2007
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1353.355 KB) | DOI: 10.14499/indonesianjpharm0iss0pp96-104

Abstract

The aim of this research was to investigate the possibility physical interaction occurred between of levodopa and benzerazide hydrochloride, which known as compounds used in combination for the the antiparkinson. The “cold contact methods” was used which was developed from Kofler contact method’s (hot stage methods). Thermograms Differential Scanning Calorimetry and difractograms X-Ray indicated that levodopa – benzerazide hydrochloride tent to form a peritecticum-molecular compound physical interaction with eutectics point at 4:6 and 9:1 molar ratios.Key words: benzerazide hydrochloride, cold contact method, levodopa, physical interaction.
Radioiodination of andrographolide and its biodistribution in mice for inflammatory tracer Levita, Jutti; A., Cahya Nova; Nawawi, As’ari; Ibrahim, Slamet
INDONESIAN JOURNAL OF PHARMACY Vol 21 No 4, 2010
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (489.965 KB) | DOI: 10.14499/indonesianjpharm0iss0pp258-265

Abstract

Andrographolide,  a  bioactive  component  of  Andrographis  paniculata (Burm.F)  Nees,  is  the  major  lactone  diterpenoidal  bicyclic  constituent  in  this plant  which  has  proven  to  exert  anti-inflammatory  activity  in  vitro  which  was occurred via  several mechanism, e.g inhibition of inducible nitric oxide synthase (iNOS),  inhibition  of  radical  oxygen  species,  and  inhibition  of  NF-kappaB activation. The labeling with radionuclide is often used for therapy, detection and quantification of metabolites in the body. Even though the metabolites are very low  in  concentration  they  can  be  detected  by  the  energy  they  emitted. Radionuclide  can  be  used as  radiotracer  to  detect  whether  drug  really interacts with  its  target.  The  objective  of  this  research  is  to  synthesize 131I-labelled andrographolide  and  to  study  its  biodistribution  in  mice  to  understand  the location  of  its  organ  target.  Indirect  radioiodination  of  andrographolide  wascarried  out  by  using  bromine  as  the  leaving  group  and  followed  by  fast iodination  at  40oC,  yielded  72.6  %  purity  of  the  labeled  compound.  Iodination was occurred through proton substitution at C-12. Then the andrographolide-131I was  injected  into  lateral  vein  of  mice’s  tail  to  study  its  biodistribution.  The compound  was  distributed  in  all organs  with the  highest  accumulation  occurred in  the  stomach  (16.87  %/gram  organ).  The  result  showed  that  inducing  the animals  with  LPS  caused  inflammation  in  the  stomach  and  increased  the production  of  prostaglandin  as  proven  by  the  distribution  of  the  radioligand  in that organ.Key words: andrographolide, radioiodination, anti-inflammatory, biodistribution
IDENTIFIKASI INTERAKSI KIMIA FISIKA PADA KOMBINASI ANTIBIOTIKA AMOKSISILINA TRIHIDRAT - KALIUM KLAVULANAT DENGAN KALORIMETER LARUTAN DAN REKRISTALISASI Nugrahani, Ilma; Asyarie, Sukmadjaja; N.S., Sundani; Ibrahim, Slamet
Pharmaceutical Sciences and Research (PSR) Vol 4, No 3 (2007)
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1320.559 KB) | DOI: 10.7454/psr.v4i3.3803

Abstract

Physico-chemical interaction of amoxicillin trihydrate - potassium clavulanate has been investigated. The method used in this experiment was solution calorimeter analysis that was collaborated by the re-crystallization observation. Amoxicillin-clavulanate were mixed on the variety ratios (0:10; 9:1; ...; 1:9; 0:10) and analyzed by solution calorimeter in the HCl 0,1 N and phosphate buffer pH 6,8 solution as the gastrointestinal liquids. After that, the re-crystallizations of amoxicillin-clavulanate on the variety ratios were analyzed by polarization microscope and digital camera. The results of observation showed that amoxicillin-clavulanate have the physical-chemical interactions in the ratios 3:7; 5:5; 7:3 respectively in both of pH models of gastrointestinal liquids.
Pengamatan Visual Tahap-Tahap Pembentukan Kristal Sistem Eutektikum Asetaminofen-Pseudoefedrin Hidroklorida Nugrahani, Ilma; Asyarie, Sukmadjaja; Soewandhi, Sundani Nurono; Ibrahim, Slamet
Pharmaceutical Sciences and Research (PSR) Vol 7, No 1 (2010)
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (713.824 KB) | DOI: 10.7454/psr.v7i1.3447

Abstract

The aim of this research was to observe the recrystallization process of two component,acetaminophen-pseudoephedrine hydrochloride in ethanol and the thermal profiles.The visual data resulted from polarization microscope observation confirmed withthermograph DSC showed that the crystal habit was represented to the intrinsic characterrepresentative by thermodynamic properties of the binary system.The visualizationprocess proved that in different molar ratios, the binary system formed crystalshabit with different and step by step showed inhibit, to form polycrystalline until atmolar ratio 6:4 formed the fines single crystals mixture which was smaller than beforeco-recrystallization. Acetaminophen was observed as monoclinic hexagonal crystalswhile pseudoephedrine hydrochloride was observed monoclinic/orthorombic forms.Keywords: acetaminophen, crystals habit, ethanol, pseudoephedrine HCl.
Polimorfisasi dan Solvatomorfi Amoksisilina Trihidrat setelah Proses beku Kering Nugrahani, Ilma; Asyarie, Sukmadjaja; Soewandhi, Sundani Nurono; Ibrahim, Slamet
Pharmaceutical Sciences and Research (PSR) Vol 7, No 2 (2010)
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (604.417 KB) | DOI: 10.7454/psr.v7i2.3452

Abstract

The polymorphismof amoxicillin has been identified after freeze drying process. Thefreeze dried amoxicillin have some specific physical properties different from their rawmaterial, that was proved by DSC, XRD, and polarize microscope. Exothermal curvefrom DSC thermogram changes to endothermal curve, diffractogram XRD changes todifferent profile, and the crystal shows different habit. All of data showed that freezedrying process improve amoxicillin to different crystal form which has higher meltingoxidation point and lower hydrate. The improvement of the crystal structure mightbe impact to change physical-pharmaceutical properties like dissolution, absorption,and change it antibiotic potency.Keywords: amoxicillin trihydrate, freeze drying, polymorphism.
Inhibitory Activity of Andrographolide and Andrograpanin on the Rate of PGH2 Formation Sumiwi, Sri A.; Halimah, Eli; Saptarini, Nyi M.; Levita, Jutti; Nawawi, Asari; Mutalib, Abdul; Ibrahim, Slamet
Pharmacology and Clinical Pharmacy Research Vol 1, No 3
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (303.747 KB) | DOI: 10.15416/pcpr.v1i3.15246

Abstract

Cyclooxygenase (COX) or prostaglandin H2 synthase (PGHS) catalyzes the conversion of arachidonic acid into prostaglandins. Nonsteroidal anti-inflammatory drugs (NSAIDs) work by inhibiting both COX-1 and COX-2 isoforms, thus disturbing this reaction. In Indonesia, Andrographis paniculata (local name: sambiloto), is empirically used to reduce inflammation by consuming the herb tea of this plant. This work studied the inhibitory activity of andrographolide and andrograpanin, diterpenoids of the plant, on the rate of prostaglandin formation. Previous works have proven that andrographolide inhibited PGE2 production in LPS-induced human fibroblast cells. This study was performed by measuring the absorbance of TMPD (tetramethyl-p-phenyldiamine) oxidized by andrographolide and andrograpanin. Acetosal was used as a control drug. The rate of PGH2 formations on either COX-1 or COX- 2 was affected by andrographolide and andrograpanin. Andrographolide and andrograpanin interact longer with COX-1 than COX-2. Andrographolide shows weak inhibition on the rate of PGH2 formation, whilst andrograpanin might be further developed for potential antiinflammatory drugs.Keywords: Andrographis paniculata, anti-inflammatory, COX, cyclooxygenase, prostaglandin
Zingiber officinale var. Rubrum Reduces the Rate of Prostaglandin Production Fikri, Fauzan; Saptarini, Nyi M.; Levita, Jutti; Nawawi, Asari; Mutalib, Abdul; Ibrahim, Slamet
Pharmacology and Clinical Pharmacy Research Vol 1, No 1
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (594.225 KB) | DOI: 10.15416/pcpr.v1i1.15200

Abstract

In Indonesia, red ginger (Zingiber officinale var. Rubrum) is usually used as topical pain reducer by directly applying the fresh rhizome. The aim of this research was to provide information regarding the pharmacological activity of Z. officinale var. Rubrum rhizome infusion on the rate of prostaglandin production. The Z. officinale var. Rubrum rhizome used in this research was purchased from Research Institute for Spices and Medicinal Plants (Balittro) Manoko Lembang, West Java, Indonesia. This research was conducted by applying TMPD (N,N,N’,N’-tetramethyl-p-phenylendiamine) as the reagent. COX-1 and COX-2 enzyme inhibitory activity can be seen from TMPD chromogenic changes that occur during PGG2 reduction to PGH2. Phytochemical screening showed that flavonoid, quinone, and monoterpenoid/sesquiterpenoid were detected in both dried rhizomes and the water extract. Three spots were detected on thin-layer chromatography system which employing chloroform-methanol (5:5) as the eluent. The rate of prostaglandin formations either by Z. officinale var. Rubrum rhizome infusion or acetylsalicylic acid on COX-1 is slower (at 25th minutes) rather than COX-2 (5th minutes). We concluded that the rhizome of Z. officinale var. Rubrum reduces the rate of prostaglandin production. The rhizome of red ginger reduces the rate of prostaglandin production, which is slower in COX-1 than in COX-2. This plant could be further developed as anti-inflammatory drug candidate.Keywords: acetosal, antiinflammation, cyclooxygenase, NSAIDs, red ginger