Bina Ikawati
Research and Development Animal Borne Diseases Control Unit, Banjarnegara

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ANALISIS KARAKTERISTIK LINGKUNGAN PADA KEJADIAN LEPTOSPIROSIS DI KABUPATEN DEMAK JAWA TENGAH TAHUN 2009 Ikawati, Bina; Nurjazuli, Nurjazuli
MEDIA KESEHATAN MASYARAKAT INDONESIA Vol 9, No 1 (2010): MKMI
Publisher : Fakultas Kesehatan Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.56 KB) | DOI: 10.14710/mkmi.9.1.33-40

Abstract

Leptospirosis is a zoonosis disease caused by Leptospira bacteria and transmitted to human by contact withcontaminated animal urin or contaminated environment. Leptospirosis cases in Demak district increased for4 year later. Until April 15th 2008, there ware 62 leptospirosis cases with Case Fatality Rate =9,67%. The aimof this research was to determine environmental characteristic related to leptospirosis case in Demak district.It was an observational research using a case control design with 44 cases and 44 controls. Cases wereleptospirosis diagnosed by leptotek dri dot in Demak Health District Agency. Controls were neighbour ofleptospirosis cases who didn?t have clinically symptom of leptospirosis, with age and sex at inclusion criteria.Laboratory diagnosis with leptotek dri dot had done for controls who agreed to sign inform consent. Datawere analyzed using chi square test at ?=5%. Variable with p value<0,25 would be continued with multivariattest using Regressi Logistic - Backward Likehood Ratio method.The result of this research showed that there ware 10 controls with no clinical diagnosis but positive atleptotek dri dot diagnosis. At statistical analysis 10 cases and 10 controls dropped. Univariat analysis showedcontrols and cases have environmental risk factor and knowledge, attitude, practice about leptospirosissimilarly. Bivariat analysis showed there was no relationship between environmental characteristic andknowledge, attitude, practice with leptospirosis. Test of water sample had been done at 8 location. One ofthem of creek showed positif contain Leptospira sp. Rat trapped had been done at four location, showed trapsucces about 8,7%-29,8%. Eventhough environment factor and knowledge, attitude, practice showed therewere no relationship with leptospirosis statistically but water from creek that positif Leptospira sp, watersuffused and highly of trap succes at rat trapped were very conducive for leptospirosis transmission.Keywords : environment, leptospirosis, Demakpermalink: http://ejournal.undip.ac.id/index.php/mkmi/article/view/2912 
ANALISIS KARAKTERISTIK LINGKUNGAN PADA KEJADIAN LEPTOSPIROSIS DI KABUPATEN DEMAK JAWA TENGAH TAHUN 2009 Ikawati, Bina; Sulistiyani, Sulistiyani; Nurjazuli, Nurjazuli
Jurnal Kesehatan Lingkungan Indonesia Vol 8, No 2 (2009): Oktober 2009
Publisher : Master of Environmental Health Study Program, Faculry of Public Health, Faculty of Public

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (246.762 KB) | DOI: 10.14710/jkli.8.2.39 - 46

Abstract

ABSTRACT Background: Leptospirosis is a zoonosis disease caused by Leptospira bacteria and transmitted to human by contact with contaminated animal urin or contaminated environment. Leptospirosis cases in Demak district increased for 4 year later. Until April 15th 2008, there were 62 leptospirosis cases with CFR=9,67%. The aim of this research was to determine environmental characteristic related to leptospirosis incidence in Demak district. Method: It was an observational research using a case control design with 44 cases and 44 controls. Cases were leptospirosis diagnosed by leptotek dri dot in Demak Health District Agency. Controls were neighbour of leptospirosis cases who didn?t have clinically symptom of leptospirosis, with age and sex appropiate to inclusion criteria. Laboratory diagnosis with leptotek dri dot had done for controls who agreed to sign inform consent. Data were analyzed using chi square test at ?=5%. Variable with p value<0,25 would be continued with multivariat test using Regressi Logistic - Backward Likehood Ratio method. Result: The result of this research showed that there were 10 controls with no clinical diagnosis but positive at leptotek dri dot diagnosis. At statistical analysis 10 cases and 10 controls dropped. Univariat analysis showed controls and cases have environmental risk factor and knowledge, attitude, practice about leptospirosis similarly. Bivariat analysis showed there was no relationship between environmental characteristic and knowledge, attitude, practice with leptospirosis. Test of  water sample had been done at 8 location. One of them of creek showed positif contain Leptospira sp. Rat trapped had been done at four location, showed trap succes about 8,7%-29,8%. Eventhough environment factor and knowledge, attitude, practice showed there were no but water from ditch that positif Leptospira sp, significant association and highly succes at rat trapped were concidered to be the risk of  leptospirosis transmission. Keywords : environment, leptospirosis, Demak
Larvicidal Effect of Mixture of Beauveria bassiana Crude Metabolite and Chitinase Enzyme against Aedes aegypti Larvae Widiastuti, Dyah; Ikawati, Bina; Hadi, Upik Kesumawati
Jurnal Kesehatan Masyarakat Nasional Volume 12, Issue 4, May 2018
Publisher : Faculty of Public Health Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (749.1 KB) | DOI: 10.21109/kesmas.v12i4.1471

Abstract

Aedes aegypti is a major vector of dengue, a deadly disease causing deaths of millions of people in developing countries, both in urban and rural populations. Aedes aegypti control with chemical insecticide is widely used, and affects on a widespread insecticide resistance. Mosquito biological control was needed to replace the use of chemical insecticide. This study aimed to evaluate larvicidal effect of mixture of Beauveria bassiana crude metabolite and chitinase enzyme against Aedes aegypti larvae. An experimental study using completely Randomized Design was conducted in March-April 2016 at Banjarnegara Research and Development Unit for Zoonosis Control. Biolarvacide formula was made of mixture with 2 : 1 ratio between Beauveria bassiana crude metabolite and chitinase isolated from chitinolytic bacteria, which was propagated by the Agency’s Bacteriology Laboratory. In experimental method, 120 Aedes aegyptilarvae (3rd instar) were exposed with four concentrations of biolarvacide formula (4%, 8%, 16%, and 32%) in 3 replicates. Results showed that exposure of biolarvacide formula caused the third instar larval mortality which started on the first day of exposure. Probit analysis showed LC50 value was obtained at concentration of 53.2 ppm. This shows that larvacide formula of Beauveria bassiana and chitinase enzyme compounds are effective to be used as larvacides against Aedes aegypti larvae.
SPOT SURVEI LEPTOSPIROSIS DI KECAMATAN NGEMPLAK DAN NOGOSARI, KABUPATEN BOYOLALI, PROVINSI JAWA TENGAH Pramestuti, Nova; Ikawati, Bina; Widiastuti, Dyah
Kesmas Indonesia: Jurnal Ilmiah Kesehatan Masyarakat Vol 7 No 1 (2014): Jurnal Kesmas Indonesia
Publisher : Jurusan Kesehatan Masyarakat dan Fakultas Ilmu-Ilmu Kesehatan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRACTIn 2013 there were three leptospirosis cases reported in Boyolali. This study aimed to describe the epidemiology of leptospirosis including cases overview aboutperson, place and time, transmission history, the trap success in catching mice and rats, and species of Leptospira infected rodent in Ngemplak and Nogosari district, Boyolali. This study was a cross sectional study. Rodent traping conducted at three locations with leptospirosis cases in Ngemplak and Nogosari District, Boyolali, in April 2013. A total of 300 traps wereinstalled, 2 traps inside and 2 traps outside for each house during three days. The trapped mice and rats were identified, counted their population density and their kidneys were taken for Leptospira examainationusingPCR (Polymerase Chain Reaction) assay. Data were processed and analyzed descriptively, presented in a frequency distribution form. The result showed that transmission of leptospirosis may occured around the case? house, the river and rice fields. The highest rodentsuccess trapwas found in KismoyosoVillage (14 %). Rat species which were found consisted of R.tanezumi, and R. indica Bandicota argentiventer, there was no rat kidney samples infected with Leptospira bacteria according to PCR assay. Leptospirosis transmission in Boyolali should be noteddue to the high rat trap success and the location which was closed to the river . Key words: Survey,leptospirosis, rats Kesmasindo, Volume 7( 1 )Juli 2014, Hal 63-70
SPOT SURVEI LEPTOSPIROSIS DI KECAMATAN NGEMPLAK DAN NOGOSARI, KABUPATEN BOYOLALI, PROVINSI JAWA TENGAH Pramestuti, Nova; Ikawati, Bina; Widiastuti, Dyah
Kesmas Indonesia: Jurnal Ilmiah Kesehatan Masyarakat Vol 7 No 1 (2014): Jurnal Kesmas Indonesia
Publisher : Jurusan Kesehatan Masyarakat dan Fakultas Ilmu-Ilmu Kesehatan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRACTIn 2013 there were three leptospirosis cases reported in Boyolali. This study aimed to describe the epidemiology of leptospirosis including cases overview aboutperson, place and time, transmission history, the trap success in catching mice and rats, and species of Leptospira infected rodent in Ngemplak and Nogosari district, Boyolali. This study was a cross sectional study. Rodent traping conducted at three locations with leptospirosis cases in Ngemplak and Nogosari District, Boyolali, in April 2013. A total of 300 traps wereinstalled, 2 traps inside and 2 traps outside for each house during three days. The trapped mice and rats were identified, counted their population density and their kidneys were taken for Leptospira examainationusingPCR (Polymerase Chain Reaction) assay. Data were processed and analyzed descriptively, presented in a frequency distribution form. The result showed that transmission of leptospirosis may occured around the case? house, the river and rice fields. The highest rodentsuccess trapwas found in KismoyosoVillage (14 %). Rat species which were found consisted of R.tanezumi, and R. indica Bandicota argentiventer, there was no rat kidney samples infected with Leptospira bacteria according to PCR assay. Leptospirosis transmission in Boyolali should be noteddue to the high rat trap success and the location which was closed to the river . Key words: Survey,leptospirosis, rats Kesmasindo, Volume 7( 1 )Juli 2014, Hal 63-70