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PENGARUH MEDIA TANAM DAN PUPUK DAUN TERHADAP AKLIMATISASI ANGGREK DENDROBIUM (DENDROBIUM SP) Wardani, Sri; Setiado, Hot; Ilyas, Syafruddin
Jurnal Ilmu Pertanian KULTIVAR Vol 5, No 1 (2011)
Publisher : Jurnal Ilmu Pertanian KULTIVAR

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Penelitian ini bertujuan untuk mengetahui pengaruh jenis media tumbuh aklimatisasi dan jenis pupuk daun terhadap pertumbuhan pada proses aklimitasiasi anggrek Dendrobium sp. Penelitian ini dilaksanakan di Laboratorium Pemuliaan Tanaman, Fakultas Pertanian, Universitas Sumatera Utara, Medan yang dimulai dari bulan Januari sampai dengan Maret 2009. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan dua faktor. Faktor pertama adalah jenis media tumbuh aklimatisasi (arang sekam, cocopeat, dan campuran antara arang sekam dan cocopeat). Faktor yang kedua adalah jenis pupuk daun (pupuk majemuk foliar I dan pupuk majemuk foliar II). Parameter yang diamati adalah persentase hidup, pertambahan tinggi planlet, pertambahan diameter batang, pertambahan jumlah daun, pertambahan berat total, saat muncul tunas, jumlah tunas, berat akar, volume akar dan jumlah klorofil daun. Hasil penelitian menunjukkan bahwa interaksi jenis media tumbuh aklimatisasi dengan jenis pupuk daun berpengaruh nyata terhadap parameter saat muncul tunas dan jumlah tunas. Kata kunci: acclimitazation, orchid, Dendrobium sp
Testosterone undecanoate and depo medroxyprogesterone acetate induced azoospermia through increased expression of spermatogenic cell caspase 3 Moeloek, Nukman; Asmarinah, Asmarinah; Siregar, Nurjati C.; Ilyas, Syafruddin
Medical Journal of Indonesia Vol 17, No 3 (2008): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (615.264 KB) | DOI: 10.13181/mji.v17i3.313

Abstract

The administration of a combination of testosterone undecanoate (TU, a long-acting androgen) and depo-medroxyprogesterone acetate (DMPA) were investigated in term of suppression of rat sperm concentration in vivo to azoospermia through increasing activity of spermatogenic cell caspase 3. Adult Sprague Dawley rats received TU and DMPA of 2.5 mg and 1.25 mg, respectively, a regimen known to rapidly reduce intra testicular testosterone and to produce azoospermia within 12 weeks. Caspase 3 positive sperm cells increased compared with control levels during 6 weeks post-injection and increased further through 60 weeks. Immunohistochemistry for caspase 3 revealed that spermatocytes represented the predominant caspase 3 positive germ cells. Modest immunoreactivity for caspase-3 was localized to nuclear region of the germ cells of control and treated testes. Immunohistochemistry study revealed significantly increased caspase-3 expression in nuclei of germ cells during administration of TU+DMPA to rats. Additionally, the caspase 3 content was significantly increased in germ cells during rats were administered TU+DMPA (453.90±84.88 cells/200 seminiferous tubules) and caspase 3 significant increase in immunoreactivity was localized to the nuclei of spermatogonia, spermatocytes, and spermatids. Taken together, these results indicated that azoospermia due to reduced intratesticular testosterone concentration was caspase-3 activation dependent and suggested that the increase in active caspase-3 in the nucleus may be involved in the induction of decreased sperm production. (Med J Indones 2008; 17: 149-56)Keywords: TU, DMPA, sperm concentration, germ cells
Anticancer potency of black sea cucumber (Holothuria atra) from Mentawai Islands, Indonesia Satari, Mieke Hemiawati; Arma, Utmi; Ilyas, Syafruddin; Handayani, Dian
Padjadjaran Journal of Dentistry Vol 29, No 1 (2017): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (148.217 KB) | DOI: 10.24198/pjd.vol29no1.11654

Abstract

Introduction: The source of bioactive compounds believed to have strong anticancer potency is derived from sea cucumber. Black sea cucumber (Holothuria atra) is a dominant species in Mentawai Islands, West Sumatera, Indonesia. Key factor compound that acts as anticancer in sea cucumber extract is tritepenoid also known as Frondoside A. The purpose of this study was to determine the effectiveness of the active compound taken from black sea cucumber as anticancer. Methods: Methods used was experimental laboratory. Anticancer activity of black sea cucumber was determined by using the MTT assay cytotoxic test to measure the IC50. Cytotoxic test was conducted with Supris clone-1 (SP-C1) derived from cloned lymphadenopathy of patients with oral squamous cell carcinoma on lingual part. Results: It was found that the compounds contained in black sea cucumber has strong anticancer activity (IC50 = 5.25 μL/mL) against SP-C1. Conclusion: Black sea cucumber extract has strong cytotoxic effects against cancer cells SP-C1, therefore black sea cucumber extract potentially developed as a new source of cancer medicine.
Pengaruh Ekstrak Methanol Biji Pare (Momordica charantia) dan DMPA Terhadap Jumlah Sel purkinje Cerebellum Mencit (Mus musculus L.) ILYAS, SYAFRUDDIN; HUTAHAEAN, SALOMO; NURSAL, NURSAL
Prosiding Seminar Biologi Prosiding Seminar Nasional From Basic Science to Comprehensive Education
Publisher : Prosiding Seminar Biologi

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Perubahan jumlah sel purkinje cerebellum mencit dapat dijadikan sebagai indikator terjadinya gangguan terhadap otak kecil pada pemberian ekstrak methanol biji pare (Momordica carantia L.) dan DMPA. Metode eksperimen digunakan untuk menentukan perbedaan yang terjadi pada tiap kelompok kontrol dan pemberian lama pemberian ekstrak biji pare dan DMPA. Kelompok kontrol terbagi dalam K0, K1, dan K2 dengan waktu berturut-turut 0, 4, dan 8 minggu. Kelompok perlakuan terdiri dari (P0) biji pare 0 minggu secara oral dan DMPA intramuskular (@ 6 jam), (P1) biji pare dan DMPA (@4 minggu), (P2) biji pare dan DMPA (@8 minggu). Masing-masing kelompok terdiri dari 5 mencit sehingga total mencit jantan adalah 30 ekor. Dosis ekstrak metanol biji pare adalah 5mg/10g berat badan mencit yang diberikan secara oral) (Yama et al. 2011). Sedangkan dosis DMPA sebesar 0,175 mg/ekor mencit yang diberikan secara intramuskular (Ilyas 2014). Hasil penelitian menunjukkan tidak adanya perbedaan yang nyata  (p>0,05) antara kontrol dan perlakuan pada 0, 4, dan 8 minggu terhadap jumlah sel purkinje cerebellum mencit. Disimpulkan bahwa pemberian ekstrak metanol biji pare dan DMPA aman terhadap perbedaan jumlah cerebellum mencit. Kata kunci: Sel purkinje, Momordica charantia L., DMPA, Mus musculus L. 
NONPOROUS CHITOSAN/COLLAGEN SCAFFOLD FOR SKIN TISSUE ENGINEERING Suryati, .; Agusnar, Harry; Gea, Saharman; Ilyas, Syafruddin
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 2 (2012): Engineering
Publisher : Syiah Kuala University

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This study aimed to determine film characteristics of chitosan/collagen scaffold for tissue engineering applications. Scaffold prepared using freeze drying method. Surface structure and biological testing chitosan/collagen scaffold crosslinking reagent addition Glutaraldehide studied using Scanning Electron Microscopy test (SEM) and Microscope inverted. Variations in the ratio of chitosan/collagen (10:0, 8:2, 7:3, 6:4, 5:5 4:6, 3:7, 2:8, 0:10), and treated with a crosslinking reagent 0.25% of Glutaraldehyde (GA) of the total weight of the polymer. The next process dissolving and mixing, followed by printing in glass moulds (7,5x7,5) with a thickness of 5 mm. This was followed by the freezing and drying with a freeze dryer. Scaffold chitosan/collagen ratio of 80:20 and a concentration of 0.25% GA showed growth of human skin fibroblast cells most and nonporous surface structure. This study is part of a study of the processing of chitosan/collagen scaffold for applications in tissue engineering
Hubungan C-Reaktif Protein dan Kondisi Udara Tercemar PM10 Dengan Lama Menetap Lansia (Studi Kasus di Kelurahan Kampung Lalang Kota Medan Tahun 2013) Pasaribu, Sumihar; -, Hidayati; Ilyas, Syafruddin
Jurnal Pendidikan Kimia (JPKim) Vol 7, No 3 (2015): Desember 2015
Publisher : Jurnal Pendidikan Kimia (JPKim)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkim.v7i3.4256

Abstract

Abstract. The decrease in air quality has long been a public health problem, particularly in the industrialized countries that has many factories and motor vehicles. The high level of air pollution in the city will affect human health in the city, and will ultimately reduce the quality of human life. The Medan city air quality periods 2011-2012  recorded data show that at Terminal Pinang Baris passed the quality standard of 150 μg/m3 for PM10 according to national standar in PP.No.41 1999. There is still plenty of evidence of the extent to which exposure to particulate matter is associated with changes in the level of systemic inflammation in the general population in the city of Medan. Exposure to air pollution is associated with increased levels of C-Reactive Protein (CRP) is believed to trigger systemic inflammation. Particle Matter (PM10) is the pollutant in the air resulting from the activity of motor vehicles, has a relationship with an increase in C-Reactive Protein. Ultimately will lead to various diseases in humans at all age levels. In cross-sectional study was conducted on 50 elderly volunteers who have been residing more than 3 years and close to the center of the air pollution in the this location. After obtaining approval from the ethics committee, then covers anamneses examination, physical examination and venous blood samples for CRP concentrations examined in the laboratory. Research result show that there are relationship between long-time resident with increasing concentrations of CRP for the elderly with statistical chi-square test and Fischer exact test. It is implies that this case enhancement factors for coronary heart disease risk in elderly residing in the study area. Keywords: CRP, long-time resident, PM10, elderly
Effects of Bean Ethanol Extract (Phaseolus Vulgaris L.) on Superoxide Dismutase Activity in Wistar Mice (Micetus Norvegicus) Hyperglycemia Sinaga, Ade Putra Fratama; Ilyas, Syafruddin; Anggraini, Dwi Rita
Indonesian Journal of Medicine Vol 2, No 2 (2017)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Background: Aloxan tetrahyd micee causes free radicals and higher reactive oxidative species (ROS) production, being able to lead to reduce insulin secretion from pancreatic β cells and receptor sensitivity in insulin-receptor cells resulting in hyperglycemia conditions. Bean extract ethanol can reduce oxidative stress. The purpose of this study was to determine bean’s ethanol extract effectiveness in lowering blood sugar levels and increasing the activity of superoxide dismutase (SOD) enzyme.Subjects and Methods: This was a randomized controlled trial (RCT) study. The samples were mice (Micetus norvegicus) of wistar strains randomized in five groups: P1-negative control, positive P2-control, P-3 ethanol extract group of 200mg / kgBW, P4-group 400mg / kgBW, P5-group 600 mg / kgBW. Dependent variables are blood sugar (mg/ dL) and superoxide dismutase enzymes. The independent variable is the bean extract of ethanol. Differences in blood sugar levels and superoxide enzyme levels of intergroup dismutase were tested by Anova test, followed by post hoc test, to determine the effectiveness of bean extract ethanol.Results: This study showed a statistically significant decrease in blood glucose levels after ethanol extract of green beans 600 mg / kg BW from day 14 to day 28 (p <0.05). This study did not show a decrease in blood sugar levels after ethanol extract of green beans 200 mg / kgBW and 400 mg / kgBW from day 7 until day 28 (p> 0.05). Giving ethanol extract of green beans did not affect the activity of superoxide dismutase enzyme (p = 0.830).Conclusion: Giving ethanol extract of green beans 600 mg / kgBW effectively decrease blood sugar in male wistar mice (Micetus norvegicus) from day 14 to day 28. Giving ethanol extract of green beans 200 mg/ kgBW, 400 mg / kgBW, and 600 mg / kgBW, is not effective to increase the activity of superoxide dismutase enzyme.Keywords: Phaseolus Vulgaris L., blood sugar level, superoxide dismutase enzymeCorrespondence: Ade Putra Fratama Sinaga. Masters Program on Biomedics, Faculty of Medicine, North Sumatra University. Email: adeputra535@ymail.com.Indonesian Journal of Medicine (2017), 2(2): 139-145https://doi.org/10.26911/theijmed.2017.02.02.08
Effect of Lawsonia inermis Linn. Ethanol Extract on the Superoxyde Dismutase Activity in Hyperglycemic Rattus norvegicus Antika, Maya Anjelir; Ilyas, Syafruddin; Sari, Mutiara Indah
Indonesian Journal of Medicine Vol 2, No 2 (2017)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Background: Alloxan causes experimental diabetic conditions (hyperglycemia) in experi­mental animals through oxidation and the formation of free radicals (oxidative stress) that damage pancreatic β cells. The ethanol extract of the henna leaves can decrease oxidative stress. The purpose of this study is to know the effect of ethanol extract of henna leaves to decrease blood sugar level and increase superoksida enzyme activity.Subjects and Methods: This study is an experimental study. The study design of posttest only control group design, using mice (Rattus norvegicus) strains of wistar, was randomized in five groups: negative control, positive control, group obtaining ethanol extract of henna leaves 200 mg/kg BW, 400 mg/kg BW and 600 mg/kg BW.Results: This study showed a significant reduction in blood glucose levels when compared to the control group. Superoxide dismutase enzyme activity increased but was not statistically significant.Conclusion: Ethanol extract of henna leaves 400 mg/kg BW significantly decreased mice blood glucose level, there was no significant difference in the increase of superoxide dis­mutase enzyme activity.Keywords: Alloxan, antioxidant, flavonoids, hyperglycemia, oxidative stress.Correspondence: Maya Anjelir Antika. Masters Program of Biomedic, Faculty of Medicine, University of North Sumatera.Indonesian Journal of Medicine (2017), 2(2): 79-85https://doi.org/10.26911/theijmed.2017.02.02.01
Hubungan C-Reaktif Protein dan Kondisi Udara Tercemar PM10 Dengan Lama Menetap Lansia (Studi Kasus di Kelurahan Kampung Lalang Kota Medan Tahun 2013) Pasaribu, Sumihar; Hidayati, Hidayati; Ilyas, Syafruddin
JURNAL PENDIDIKAN KIMIA (JPKim) Vol 7, No 3 (2015): Desember 2015
Publisher : JURNAL PENDIDIKAN KIMIA (JPKim)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkim.v7i3.4256

Abstract

Abstract. The decrease in air quality has long been a public health problem, particularly in the industrialized countries that has many factories and motor vehicles. The high level of air pollution in the city will affect human health in the city, and will ultimately reduce the quality of human life. The Medan city air quality periods 2011-2012  recorded data show that at Terminal Pinang Baris passed the quality standard of 150 μg/m3 for PM10 according to national standar in PP.No.41 1999. There is still plenty of evidence of the extent to which exposure to particulate matter is associated with changes in the level of systemic inflammation in the general population in the city of Medan. Exposure to air pollution is associated with increased levels of C-Reactive Protein (CRP) is believed to trigger systemic inflammation. Particle Matter (PM10) is the pollutant in the air resulting from the activity of motor vehicles, has a relationship with an increase in C-Reactive Protein. Ultimately will lead to various diseases in humans at all age levels. In cross-sectional study was conducted on 50 elderly volunteers who have been residing more than 3 years and close to the center of the air pollution in the this location. After obtaining approval from the ethics committee, then covers anamneses examination, physical examination and venous blood samples for CRP concentrations examined in the laboratory. Research result show that there are relationship between long-time resident with increasing concentrations of CRP for the elderly with statistical chi-square test and Fischer exact test. It is implies that this case enhancement factors for coronary heart disease risk in elderly residing in the study area. Keywords: CRP, long-time resident, PM10, elderly
Anticancer potency of black sea cucumber (Holothuria atra) from Mentawai Islands, Indonesia Satari, Mieke Hemiawati; Arma, Utmi; Ilyas, Syafruddin; Handayani, Dian
Padjadjaran Journal of Dentistry Vol 29, No 1 (2017): March
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (148.217 KB) | DOI: 10.24198/pjd.vol29no1.11654

Abstract

Introduction: The source of bioactive compounds believed to have strong anticancer potency is derived from sea cucumber. Black sea cucumber (Holothuria atra) is a dominant species in Mentawai Islands, West Sumatera, Indonesia. Key factor compound that acts as anticancer in sea cucumber extract is tritepenoid also known as Frondoside A. The purpose of this study was to determine the effectiveness of the active compound taken from black sea cucumber as anticancer. Methods: Methods used was experimental laboratory. Anticancer activity of black sea cucumber was determined by using the MTT assay cytotoxic test to measure the IC50. Cytotoxic test was conducted with Supris clone-1 (SP-C1) derived from cloned lymphadenopathy of patients with oral squamous cell carcinoma on lingual part. Results: It was found that the compounds contained in black sea cucumber has strong anticancer activity (IC50 = 5.25 μL/mL) against SP-C1. Conclusion: Black sea cucumber extract has strong cytotoxic effects against cancer cells SP-C1, therefore black sea cucumber extract potentially developed as a new source of cancer medicine.