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Journal : Jurnal Sumberdaya Hayati

Bacteria as Greenhouse Gases Reducing Agents from Paddy Plantation MUTTAQIN, MAFRIKHUL; ., MIFTAHUDIN; RUSMANA, IMAN
Jurnal Sumberdaya Hayati Vol 2, No 2 (2016)
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.2.2.%p

Abstract

High methane oxidation activity of local isolated methanotrophic bacteria have a potent as methane gases reducing agent while combined with nitrogen fixing bacteria as paddy biofertilizer. The aim of the research was to evaluate the effectiveness of the bacteria as methane gases reducing agent and biofertilizer in paddy plantation. The research was arranged in a completely randomized design consisted of fertilizer types and watering system treatments with four replicates. The research showed that paddy shoot length was not affected by the treatment. On the other hand, both plant freshand dry weight, as well as the number of productive tiller were affected by interaction of fertilizer types and watering system. Fertilizer types affected grain per panicle and methane flux after fertilization. In the end of paddy vegetative stage, bacterial fertilizers were capable to reduce methane emission in different rate. The different result in methane flux was likely due to the interaction between soil local microorganisms and soil chemical component. 
Karakterisasi Enzim Pendegradasi AHL dari Bacillus cereus INT1c dan Bacillus sp. NTT3a RATNANINGTYAS, SUSI; RUSMANA, IMAN; AKHDIYA, ALINA
Jurnal Sumberdaya Hayati Vol 3, No 1 (2017)
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.3.1.%p

Abstract

Some of Gram-negative bacteria perform a phenomenon called quorum sensing (QS) to activate certain phenotypes such as pathogenicity. The bacterial cells performing QS produce N-acyl homoserine lactone (AHL) as signal molecules to communicate within a population. These molecules can be degraded by the enzyme, i.e. AHL lactonase. This study aimed to characterize the activity of AHL lactonase from Bacillus cereus INT1c and Bacillus sp. NTT3a in different pH and temperature levels. Both strains produce AHL-lactonase that could be found in intracellular and extracellular extracts. The dialysis process of extracellular AHL-lactonase of INT1c significantly increased the specific activity from 5.91 to 29.96, different from an extracellular enzyme of NTT3a that slightly increased from 4.08 to 5.39. Generally dialyzedAHL-lactonase of both B. cereus INT1c and Bacillus sp. NTT3a had activity in wide pH range with better activity in acidic pH and were not stable in high temperature with the highest activity at 30-40 oC.