AGUSTIN INDRAWATI
Departemen Ilmu Penyakit Hewan dan Kesehatan Masyarakat Veteriner FKH IPB

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PENENTUAN PATOTIPE MOLEKULER VIRUS NEWCASTLE DISEASE: ISOLAT LAPANG DI TIGA WILAYAH KABUPATEN JAWA TIMUR Kurnianingtyas, Erin; Setiyaningsih, Surachmi; Indrawati, Agustin
Acta VETERINARIA Indonesiana Vol. 5 No. 1 (2017): Januari 2017
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (286.086 KB) | DOI: 10.29244/avi.5.1.8-15

Abstract

Tujuan penelitian ini adalah mengkaji keberadaan dan karakteristik molekuler virus newcastle disease (VND) di tiga wilayah Kabupaten di Jawa Timur. Sampel usapan kloaka diambil dari 289 ekor unggas (262 ekor ayam dan 27 ekor bebek) pekarangan, pedagang di pasar unggas hidup, peternak, dan pengepul di wilayah Kabupaten Probolinggo, Situbondo, dan Bondowoso. Sampel usap kloaka ditumbuhkan pada telur ayam berembrio (TAB) dan deteksi virus dengan Real Time Reverse Transcription Polymerase Chain Reaction (rRT-PCR) matrix (M). Patogenisitas virus ditentukan melalui rRT-PCR Fusion (F) dan sekuen gen F. Sejumlah delapan isolat lapang VND yang didapat, semuanya mempunyai afinitas lebih tinggi dengan serum Komarov dibandingkan dengan B1. Hal tersebut sesuai dengan reaksi positif yang ditunjukkan oleh rRT-PCR F. Analisis sekuen nukleotida menegaskan adanya motif asam amino multibasic pada cleavage site protein F, terbukti dari enam isolat asal ayam dan satu isolat asal bebek memiliki motif 112RRQKRF117, sedangkan satu isolat asal bebek lainnya mempunyai motif112RRRKRF117.
DETEKSI PENYAKIT BOVINE VIRAL DIARRHEA PADA SAPI POTONG IMPOR MELALUI PELABUHAN TANJUNG PRIOK Primawidyawan, Aditya; Indrawati, Agustin; Lukman, Denny Widaya
Acta VETERINARIA Indonesiana Vol. 4 No. 1 (2016): Januari 2016
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (267.354 KB) | DOI: 10.29244/avi.4.1.7-13

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Penelitian ini bertujuan untuk melakukan suatu kajian serologis tentang penyakit bovine viral diarrhea (BVD) dan mendeteksi adanya kaitan pemeliharaan kandang sebagai faktor risiko sumber penularan penyakit BVD pada sapi potong impor. Pengujian screening awal mengggunakan ELISA (enzyme linked immunosorbant assay) Antibodi BVD terhadap 100 sampel serum darah sapi, dan ditemukan 63 positif terhadap adanya antibodi anti BVD. Selanjutnya dilakukan pengujian lanjutan ELISA Antigen BVD dan hasilnya seluruh sampel negatif terhadap Antigen BVD. Hasil positif uji ELISA terhadap antibodi BVD mengindikasikan bahwa sampel mengandung antibodi anti BVD akibat pernah terinfeksi oleh virus BVD secara sementara (transient) atau melalui vaksinasi. Berdasarkan dokumen health certificate dari negara asal tidak terdapat informasi yang jelas terhadap perlakuan vaksinasi BVD pada sapi potong impor. Oleh karena itu diperlukan pemeriksaan screening di negara Indonesia untuk mendeteksi dan melakukan usaha preventif mencegah penyebaran di feedlot. Faktor-Faktor yang mempengaruhi kejadian hasil ELISA antibodi positif BVD, terkait dengan penyebaran penyakit BVD selama dalam masa pemeliharaan dan penggemukkan adalah program biosekuriti pada peternakan dengan nilai (OR=3,316; CI=1,380-7,967), dan pengelolaan limbah kandang dalam peternakan dengan nilai (OR=2,667; CI=1,105-6,434). Hasil ini menunjukkan ada asosiasi antara kedua faktor yang ada pada peternakan dengan kejadian penyakit BVD.Kata kunci: BVD, ELISA antibodi dan antigen, faktor risiko. (Detection and Risk Factors Study of Bovine Viral Diarrhea in Cattle Imports at Tanjung Priok Port)This research was a serological study on bovine viral diarrhea (BVD) and also to detect the relevance of maintenance farm management as a risk factor on the spreads of BVD. The initial screening test was performed using antibody capture enzyme linked immunosorbent assay (ELISA) to BVD on 100 cattle blood serum samples. The screening test showed that of 63 samples were positive to BVD antibody and 37 samples were negative to BVD antibody. The next screening test was performed using antigen capture ELISA to BVD and all samples showed negative results on BVD antigen. The results of the ELISA test positive for antibodies to BVD indicates that the samples examined anti-BVD antibodies due to BVD virus had been infected by a temporary (transient) or vaccination. Based on the document health certificate from the country of origin there is no clear information on the treatment of BVD vaccination on imports of beef cattle. So, we need a screening examination in the country of Indonesia to detect and perform preventive measures to prevent the spread in feedlots. Relevant factors that affected the occurrence of positive result on BVD antibody detection was farm biosecurity programs with odds ratio (OR) value of 3.316 and confidential interval (CI) value of 1.380-7.967. Further relevant factor was caging waste management with OR value of 2.667 and CI value of 1.105-6.434. There were statistically significant differences (p<0.05) between farm biosecurity programs and caging waste management related to BVD disease incidence.Keywords: BVD, ELISA antibodies and antigen, risk factors
DETEKSI RESIDU HORMON TRENBOLON ASETAT PADA SAPI SIAP POTONG IMPOR ASAL AUSTRALIA Danial, Rifky; Latif, Hadri; Indrawati, Agustin
Acta VETERINARIA Indonesiana Vol. 3 No. 2 (2015): Juli 2015
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.149 KB) | DOI: 10.29244/avi.3.2.70-76

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Trenbolon asetat (TBA) merupakan hormon penggertak pertumbuhan yang diimplankan ke sapi untuk meningkatkan berat badan dan mengefisiensi konversi pakan. Penggunaan TBA dapat meninggalkan residu dalam urin dan dapat menyebabkan efek negatif. Tujuan dari penelitian ini adalah untuk menganalisis keberadaan residu TBA dalam urin sapi siap potong impor dari Australia. Ukuran sampel dihitung dengan menggunakan rumus deteksi penyakit dan sampel dipilih secara acak. Sebanyak 60 sampel dianalisis menggunakan enzim-linked immunosorbent assay (ELISA). Tes menunjukkan bahwa sebanyak 100% urin sapi siap potong dari Australia mengandung residu TBA dengan konsentrasi yang bervariasi. Konsentrasi residu TBA < 2 part per billion (ppb) terdeteksi pada 37 sampel (61,67%), konsentrasi residu TBA 2-4 ppb terdeteksi pada 7 sampel (7%), dan konsentrasi residu TBA > 4 ppb terdeteksi pada 16 sampel (26,67%). Hasil positif menunjukkan bahwa sapi potong asal Australia mengandung residu hormon trenbolon asetat (TBA).Kata kunci: ELISA, residu, sapi potong impor, trenbolon asetat, urin (Detection of Trenbolone Acetate Hormone Residues in Imported Slaughter Cattle from Australia)Trenbolone acetate (TBA) is a growth hormone promoter which is implanted into cattle to increase weight gain and feed conversion efficiency. The use of TBA can leave residue in urine and may cause negative effects. The objective of this research was to analyze the presence of the TBA residue in imported slaughter cattle urine from Australia. Cattle urine samples were collected from Animal Quarantine Installation. Sample size was calculated using the formula of detect disease and selected by random sampling. A total of 60 samples of cattle urine were analyzed for level of trenbolone acetate residues by using enzyme-linked immunosorbent assay (ELISA) method. The test showed that positive results in all of urine samples (100%) of slaughter cattle imported from Australia with variation in TBA residues concentrations. The concentration of residual TBA < 2 ppb were detected in 37 samples (61.67%), the residual concentration of TBA 2-4 ppb were detected in 7 samples (7%), and the concentration of residual TBA > 4 ppb were detected in 16 samples (26.67%). Total of 60 urine samples contained TBA residues. The presence of TBA residues with concentration above 4 ppb was 16 samples (26.7%). Positive results in the samples was indicated the Australian cattle contains trenbolone acetate (TBA) residue.Keywords: ELISA, residue, imported slaughter cattle, trenbolone acetate, urine
DETEKSI SALMONELLA SPP. PADA TELUR AYAM KONSUMSI YANG DILALULINTASKAN MELALUI PELABUHAN TENAU KUPANG Nugroho, Susanto; Purnawarman, Trioso; Indrawati, Agustin
Acta VETERINARIA Indonesiana Vol. 3 No. 1 (2015): Januari 2015
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.83 KB) | DOI: 10.29244/avi.3.1.16-22

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Salmonelosis adalah salah satu penyakit food-borne bakterial zoonotik yang paling penting di seluruh dunia. Salmonella spp. adalah penyebab salmonelosis akibat konsumsi makanan berbahan dasar unggas dan produk unggas yang terkontaminasi. Unggas dan telur ayam dianggap merupakan salah satu reservoir Salmonella spp. yang paling penting. Salmonella spp. ditularkan melalui rantai makanan dan akhirnya menular ke manusia. Meningkatkan keamanan produk unggas dengan cara deteksi dini terhadap food-borne patogen merupakan komponen penting untuk membatasi kontaminasi Salmonella spp.. Metode deteksi dan identifikasi Salmonella spp. merupakan strategi yang dirancang untuk mencegah kontaminasi unggas dan produk unggas. Tujuan dari penelitian ini adalah untuk mendeteksi Salmonella spp. dari telur ayam yang berasal dari 4 pengirim telur menggunakan metode konvensional. Jumlah sampel dihitung dengan menggunakan rumus menduga prevalensi dan diambil menggunakan metode acak berlapis. Analisis data hasil positif Salmonella spp. dilakukan secara deskriptif. Berdasarkan hasil pengujian dari 270 sampel, 5 pengujian dengan metode konvensional positif Salmonella spp.. Berdasarkan hasil pengujian positif kontaminasi Salmonella spp. pada telur ayam maka diperlukan evaluasi terhadap pengiriman telur ayam konsumsi antar pulau.Kata kunci: kontaminasi, Salmonella spp., metode konvensional, telur ayam. (Detection of Salmonella spp. in Commercial Hen Eggs Entering through Tenau Port Kupang)Salmonellosis is one of the most important food-borne bacterial zoonotic diseases worldwide. Salmonella spp. are causative agent of salmonellosis associated with contaminated commercial poultry and poultry product. Poultry and eggs are considered one of the most important Salmonella spp. reservoirs. Salmonella spp. were able to pass through the food chain and ultimately transmitted to humans. Improving safety of poultry products by early detection of food-borne pathogens would be considered an important component for limiting exposure to Salmonella contamination. Detection and identification method for Salmonella spp. are considered to be an important component of strategies designed to prevent poultry and poultry product. The aims of the study were to detect Salmonella spp. from hen eggs collected from 4 exporters using conventional method. Samples size were calculated using estimates prevalence formula and selected by stratified random sampling. Data regarding the proportion of Salmonella spp. positive samples were analyzed descriptively. 270 samples, 5 test by conventional method were positive Salmonella spp.. According of positive test results Salmonella spp. contamination in hen eggs was necessary to evaluate the delivery of commercial hen eggs between islands.Keywords: conventional methods, hen eggs, Salmonella spp. contamination.
STUDI SISTEM RESPIRASI DAN KAJIAN MIKROBIOLOGIS LUMBA-LUMBA HIDUNG BOTOL INDO PASIFIK (TURSIOPS ADUNCUS) DARI PERAIRAN LAUT JAWA Mulyani, Guntari Titik; Fibrianto, Yuda Heru; Budipitojo, Teguh; Indrawati, Agustin
Acta VETERINARIA Indonesiana Vol. 2 No. 1 (2014): Januari 2014
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (980.858 KB) | DOI: 10.29244/avi.2.1.7-11

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Lumba-lumba hidung botol (bottlenose dolphin) adalah spesies lumba-lumba yang paling umum danpaling dikenal orang. Gangguan sistem respirasi pada lumba-lumba sering dijumpai, sementara jenisbakteri yang sering menyerang lumba-lumba dari perairan Laut Jawa belum pernah diteliti. Penelitianini bertujuan untuk mempelajari pemeriksaan klinis sistem respirasi lumba-lumba dan melakukan isolasibakteri serta jamur pada sistem respirasi bottlenose dolphin (Tursiops aduncus) dari Perairan Laut Jawa.Penelitian dilakukan dengan studi pustaka, pemeriksaan klinis serta isolasi sampel sistem respirasi. Swabdeep blowhole dilakukan terhadap sepuluh ekor bottlenose dolphin di PT. Wersut Seguni Indonesia.Sampel dikirim ke laboratorium Mikrobiologi FKH UGM untuk isolasi dan identifikasi terhadap bakteridan jamur. Hasil penelitian menunjukan bahwa pemeriksaan klinis lumba-lumba hanya dapat dilakukansecara inspeksi. Hasil isolasi ditemukan bahwa 5 dari 10 lumba-lumba (50%) positif Staphylococcus aureus,sedangkan jamur tidak ditemukan. Berdasarkan hasil penelitian, disimpulkan bahwa pemeriksaan klinislumba-lumba dapat dilakukan dengan cara inspeksi terhadap blowhole, tingkah laku hewan, adanya?chuff? atau ?honk?, frekuensi respirasi, sosialisasi hewan dan posisi hewan saat berenang. BakteriStaphylococcus aureus dijumpai pada sistem pernafasan lumba-lumba hidung botol dari perairan LautJawa di PT. Wersut Seguni Indonesia.
PEMANFAATAN SUPERNATAN LACTOBACILLUS PLANTARUM SEBAGAI PENGHAMBAT PERTUMBUHAN ESCHERICHIA COLI PADA DANGKE SUSU SAPI (UTILIZATION OF LACTOBACILLUS PLANTARUM SUPERNATAN AS AN INHIBITIOR OF ECHERICHIA COLI GROWTH IN COW’S MILK DANGKE Arini, Nining; Sudarwanto, Mirnawati; Sudirman, Idwan; Indrawati, Agustin
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (169.479 KB)

Abstract

Dangke is a traditional food in Enrekang, a district in South Sulawesi. Its made from buffalo?s milk orcow?s milk. Dangke could be contaminated during the process with Escherichia coli which causes diarrheain children and adults. It was known that supernatant of Lactobacillus plantarum has antibacterial capacity,and it may be used as a biopreservative agent. The research aims were to determine the minimum inhibitoryconcentration (MIC) of L. plantarum supernatant in inhibiting the growth of E. coli ATCC 25922, determinethe nutrients level of cow?s milk dangke after the addition of 1% and 2% milk fat, as well as determine theeffect of L. plantarum supernatant and cow?s milk fat addition into dangke inhibited the growths of pathogenicbacteria of E. coli ATCC 25922. MIC value was determined based on the value of the lowest concentrationof supernatant that shown with no any bacteria growth in the media. Data of pathogenic bacteria growth analyzed with analysis of variance test with a 2x2 factorial design, which 1st factor was the addition of L.plantarum supernatant (with or without addition of supernatant) and the second factor was the additionof fat content (1% and 2%) and time observation was made on days 0, 2nd, 4th, 6th, and 8th. Resultsshowed that the filtrate of fermented L. plantarum was able to inhibit the growth of E. coli ATCC 25922in vitro and had 10% minimum inhibitory concentration (MIC). Level of fat and protein in dangke whichadded 1% cow?s milk fat, was higher than with 2% cow?s milk fat. The L. plantarum supernatant is provedto be able to inhibit the growth of E. coli ATCC 25922. Therefore, it is potentially used as a naturalbiopreservative agent in making dangke.
Monoclonal antibodies to human IgM Sjahrurachman, Agus; Indrawati, Agustin; Ernawati, Betty; Ibrabim, Fera; Mardiastuti, Mardiastuti
Medical Journal of Indonesia Vol 8, No 4 (1999): October-December
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (512.461 KB) | DOI: 10.13181/mji.v8i4.714

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[no abstract available]
DETEKSI MOLEKULER DAN KERAGAMAN VIRUS NEWCASTLE DISEASE PADA AYAM KAMPUNG DI WILAYAH ACEH D, Darniati; Setiyaningsih, Surachmi; Indrawati, Agustin
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (540.562 KB) | DOI: 10.21157/j.ked.hewan.v9i2.2841

Abstract

Tujuan dari penelitian ini adalah untuk mendeteksi keberadaan virus Newcastle disease (VND) dan mengkaji keragaman dari virus terisolasi. Sampel penelitian berupa usapan kloaka dan orofaring dari 177 ekor ayam kampung yang diambil dari unggas pekarangan dan pasar unggas di 12 kecamatan dalam wilayah Kabupaten Aceh Besar dan Kota Banda Aceh. Penapisan virus dilakukan pada sampel pool dengan real-time reverse transcriptation-polymerase chain reaction (rRT-PCR) dengan target gen matriks. Inokulasi 309 sampel representasi 157 ayam asal pool positif matriks pada telur ayam berembrio spesifik pathogen free (SPF) menghasilkan 69 isolat yang berasal dari 51 ekor ayam. Sebagian besar (45,09%)ayam mengeluarkan virus melalui orofaring, 25,39% melalui kloaka dan orofaring, serta 19,61% melalui kloaka. Karakterisasi keragaman isolat dilakukan dengan uji hemagglutination inhibition (HI) menggunakan serum Komarov dan Hitchner B1, rRT-PCR gen fusi dan uji elusi. Adanya keragaman epitop permukaan virus ditunjukkan dengan titer HI yang bervariasi antar isolat, perbedaan mencapai 4 log dengan serum Komarov, dan 3 log dengan B1. Sebagian besar isolat mempunyai afinitas yang lebih tinggi terhadap serum Komarov yang mengindikasikan kecenderungan kepada galur virulen. Penentuan patogenisitas menggunakan rRT-PCR menunjukkan 73,95% isolat termasuk ke dalam galur virulen (mesogenik/velogenik), sementara dari uji elusi menunjukkan 72,46% isolat termasuk galur velogenik, 20,29% mesogenik dan 7,25% dari galur lentogenik.
DETEKSI GEN PENYANDI RESISTENSI AMPC DAN MCR-1 PADA ESCHERICHIA COLI PENYEBAB COLIBACILLOSIS UNGGAS DI SUKABUMI (DETECTION OF GENE ENCODING RESISTANCE AMPC AND MCR-1 IN ESCHERICHIA COLI CAUSES AVIAN COLIBACILLOSIS IN SUKABUMI) Indrawati, Agustin; Kurnia, Ryan Septa; Mayasari, Ni Luh Putu Ika
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (312.231 KB) | DOI: 10.19087/jveteriner.2019.20.4.495

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Antibiotic resistance has become a global problem that can threaten human and animal health. The use of antibiotics in livestock as a treatment and control of disease is often associated with the cause of the spread of resistant bacteria. Resistance bacteria are caused by presence of resistant gene resistance that can move between bacteria. This study aims to detect the presence of genes that encode resistance to ampicillin (ampC) antibiotics and colistin (mcr-1) in Escherichia coli bacteria derived from cases of colibacillosis in Sukabumi. A total of 25 isolates of E. coli archive collection of PT. Medika Animal Lab is used in this research. All isolates identified using PCR were then tested for sensitivity using the disk diffusion method and minimum inhibitory concentrations (MICs). Isolates that are resistant to ampicillin and colistin were tested for detection of ampC and mcr-1 genes using PCR. The results of the sensitivity test showed the whole isolates were resistant to ampicillin (100%) and phosphomycin (8%), but none were resistant to colistin sulphate. The total isolate E. coli successfully detected gene encoding resistance of ampC (100%). The results of sensitivity and resistance detection test showed that the whole isolates were ampicillin resistant and had the ampC resistance-encoding gene. 
KAJIAN PATOGENISITAS BAKTERI EDWARDSIELLA ICTALURI PADA IKAN PATIN PANGASIONODON HYPOPHTHALMUS Susanti, Wiwik; Indrawati, Agustin; Pasaribu, Fachriyan H
Jurnal Akuakultur Indonesia Vol. 15 No. 2 (2016): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3460.241 KB) | DOI: 10.19027/jai.15.99-107

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ABSTRACT One of major problem of striped catfish Pangasionodon hypophthalmus culture is enteric septicemia of catfish (ESC), bacterial disease of Edwardsiella ictaluri, caused of more than 50% of mortalities. This reaserch was aimed to determine pathogenicity of local isolate E. ictaluri. Thirty individu of five group fishes, 6?10 g in body weight, injected intraperitoneally with 0,1 mL of bacteria suspension of 102 cfu/mL; 104 cfu/mL; 106 cfu/mL; 108 cfu/mL; 1010 cfu/mL; and PBS as control, were culture in 18 of 60×40×45 cm3 aquarium for seven days. External organs of fish (skin and abdomen) and internal organs (liver, kidney, and brain) were examined macroscopicly and microscopicly. Internal organ sample were taken on the 5th day for histopatologic test while blood sample was on the 1st, 3rd, and 5th day after infection. Mortality rate was count to reach LD50. Clinical signs and pathology anatomy of co-infection fish showed vertical swim, petechial hemorrhage in the skin, dropsy, ascites in the abdominal cavity, pale liver and the kidney was dark red. Histopathology showed hydropic degeneration, fatty degeneration, hemorrhage and necrosis in the liver, melano macrophage center (MMC) and necrosis in the kidneys, hemorrhage, and inflammatory cell infiltrates were also found in the kidneys and brain. Decreased of hematocrit and hemoglobin values of all tread group were statistically significant different (P<0,05) compared to controls. LD50 dose was 2,8×104 cfu/mL. The result indicated that E. ictaluri was very pathogenic on striped catfish P. hypophthalmus.  Keywords: Edwardsiella ictaluri, enteric septicemia of catfish (ESC), pathogenicity, striped catfish  ABSTRAK Salah satu kendala yang dijumpai pada budidaya ikan patin Pangasionodon hypophthalmus yaitu serangan penyakit bakterial. Enteric septicemia of catfish (ESC) adalah penyakit infeksi bakteri Edwardsiella ictaluri yang dapat menyebabkan kematian ikan patin sampai >50%. Penelitian ini dilakukan untuk mengetahui patogenisitas E. ictaluri isolat lokal pada ikan patin. Masing-masing 30 ekor ikan patin ukuran 6?10 g/ekor diinjeksi secara intraperitoneal dengan 0,1 mL larutan bakteri kepadatan 102 cfu/mL; 104 cfu/mL; 106 cfu/mL; 108 cfu/mL; 1010 cfu/mL; dan PBS sebagai kontrol. Ikan dipelihara selama tujuh hari pada akuarium berukuran 60×40×45 cm3. Pemeriksaan makroskopis dan mikroskopis dilakukan terhadap organ eksternal (kulit dan abdomen) dan internal (hati, ginjal, dan otak). Pengambilan sampel organ internal untuk uji histopatologi pada hari kelima dan sampel darah untuk uji gambaran darah pada hari pertama, ketiga, dan kelima pascainfeksi. Jumlah kematian ikan dihitung untuk mendapat nilai LD50. Pengamatan gejala klinis dan patologi anatomi ditemukan ikan berenang vertikal, adanya bercak merah pada kulit, pembengkakan abdomen, asites, hati pucat, dan ginjal berwarna merah kehitaman. Hasil histopatologi terlihat terjadinya degenerasi hidropik, degenerasi lemak, melano macrophage center (MMC), nekrosa, hemoragi, dan infiltrasi sel radang pada hati, ginjal, dan otak. Penurunan nilai hematokrit dan hemoglobin pada perlakuan secara statistik berbeda nyata (P<0,05) dengan kontrol. Dosis LD50 didapat 2,8×104 cfu/mL. Hasil penelitian ini mengindikasikan bahwa E. ictaluri pada ikan patin bersifat sangat patogen. Kata kunci: Edwardsiella ictaluri, enteric septicemia of catfish (ESC), patogenisitas, patin