AGUSTIN INDRAWATI
Departemen Ilmu Penyakit Hewan dan Kesehatan Masyarakat Veteriner FKH IPB

Published : 23 Documents
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Journal : Jurnal Veteriner

PEMANFAATAN SUPERNATAN LACTOBACILLUS PLANTARUM SEBAGAI PENGHAMBAT PERTUMBUHAN ESCHERICHIA COLI PADA DANGKE SUSU SAPI (UTILIZATION OF LACTOBACILLUS PLANTARUM SUPERNATAN AS AN INHIBITIOR OF ECHERICHIA COLI GROWTH IN COW’S MILK DANGKE Arini, Nining; Sudarwanto, Mirnawati; Sudirman, Idwan; Indrawati, Agustin
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Dangke is a traditional food in Enrekang, a district in South Sulawesi. Its made from buffalo?s milk orcow?s milk. Dangke could be contaminated during the process with Escherichia coli which causes diarrheain children and adults. It was known that supernatant of Lactobacillus plantarum has antibacterial capacity,and it may be used as a biopreservative agent. The research aims were to determine the minimum inhibitoryconcentration (MIC) of L. plantarum supernatant in inhibiting the growth of E. coli ATCC 25922, determinethe nutrients level of cow?s milk dangke after the addition of 1% and 2% milk fat, as well as determine theeffect of L. plantarum supernatant and cow?s milk fat addition into dangke inhibited the growths of pathogenicbacteria of E. coli ATCC 25922. MIC value was determined based on the value of the lowest concentrationof supernatant that shown with no any bacteria growth in the media. Data of pathogenic bacteria growth analyzed with analysis of variance test with a 2x2 factorial design, which 1st factor was the addition of L.plantarum supernatant (with or without addition of supernatant) and the second factor was the additionof fat content (1% and 2%) and time observation was made on days 0, 2nd, 4th, 6th, and 8th. Resultsshowed that the filtrate of fermented L. plantarum was able to inhibit the growth of E. coli ATCC 25922in vitro and had 10% minimum inhibitory concentration (MIC). Level of fat and protein in dangke whichadded 1% cow?s milk fat, was higher than with 2% cow?s milk fat. The L. plantarum supernatant is provedto be able to inhibit the growth of E. coli ATCC 25922. Therefore, it is potentially used as a naturalbiopreservative agent in making dangke.
DETEKSI GEN PENYANDI RESISTENSI AMPC DAN MCR-1 PADA ESCHERICHIA COLI PENYEBAB COLIBACILLOSIS UNGGAS DI SUKABUMI (DETECTION OF GENE ENCODING RESISTANCE AMPC AND MCR-1 IN ESCHERICHIA COLI CAUSES AVIAN COLIBACILLOSIS IN SUKABUMI) Indrawati, Agustin; Kurnia, Ryan Septa; Mayasari, Ni Luh Putu Ika
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2019.20.4.495

Abstract

Antibiotic resistance has become a global problem that can threaten human and animal health. The use of antibiotics in livestock as a treatment and control of disease is often associated with the cause of the spread of resistant bacteria. Resistance bacteria are caused by presence of resistant gene resistance that can move between bacteria. This study aims to detect the presence of genes that encode resistance to ampicillin (ampC) antibiotics and colistin (mcr-1) in Escherichia coli bacteria derived from cases of colibacillosis in Sukabumi. A total of 25 isolates of E. coli archive collection of PT. Medika Animal Lab is used in this research. All isolates identified using PCR were then tested for sensitivity using the disk diffusion method and minimum inhibitory concentrations (MICs). Isolates that are resistant to ampicillin and colistin were tested for detection of ampC and mcr-1 genes using PCR. The results of the sensitivity test showed the whole isolates were resistant to ampicillin (100%) and phosphomycin (8%), but none were resistant to colistin sulphate. The total isolate E. coli successfully detected gene encoding resistance of ampC (100%). The results of sensitivity and resistance detection test showed that the whole isolates were ampicillin resistant and had the ampC resistance-encoding gene. 
UJI PATOGENISITAS ZOOSPORA KAPANG LAGENIDIUM GIGANTEUM TERHADAP LARVA INSTAR-2 NYAMUK AEDES AEGYPTI SKALA LABORATORIUM (PATHOGENICITY TEST OF ZOOSPORA LAGENIDIUM GIGANTEUM FUNGI AGAINST AEDES AEGYPTI LARVAE 2ND UNDER LABORATORY CONDITION) Indrawati, Agustin; Sudarwanto, Mirnawati; Tampubolon, Mangaraja Pidoli; Soejoedono, Retno Damayanti; Wibawan, I Wayan Teguh
Jurnal Veteriner Vol 12 No 1 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Dengue Haemorrhagic fever (DHF) is one of fearsome diseases in society. Incidence of the disease isincreasing. Dengue fever is caused by dengue virus and transmitted by Aedes aegypti mosquito vector.Various chemical controls have been conducted to prevent the spread of the disease, but active contents ofthe chemical controlling substances are suspected causing many negative effect, in environment, such asvector resistance, death of non target living creatures, and environmental contamination.This researchobjective was to find an alternative solution in order to control the dengue vector by using entomopathogenicfungi as biological control agent. This research was conducted by isolation and identification of fungiinfecting mosquito larvae. Macroscopic observation revealed that one of the nine isolation products wasLagenidium giganteum. The effectiveness test in laboratory showed the zoospore LD50 to Ae.aegypti larvaeof instar 2nd was 2,35 x 106 zoospore/ml, while the LD95 value was 1,35 x 107 zoospore/ml. The oosporeeffectiveness test showed LD50 was 6,7 x 102 oospore/ml and LD95 was 1,94 x 103 oospore/ml. Using LPCBdye and blue tolouidin 2,5%, the infection mechanism of L.giganteum fungi in Ae.aegypti mosquito larvawas detected. The research is concluded that the entomophatogen fungi L. giganteum was very prospectiveto be used as a biological control agent against vector of DHF.