RISA INDRIANI
Balai Besar Penelitian Veteriner

Published : 49 Documents
Articles

Infectious Bronchitis (IB) Disease and its Control in Chicken Indriani, Risa; ., Darminto
WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences Vol 9, No 2 (1999)
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.556 KB) | DOI: 10.14334/wartazoa.v9i2.723

Abstract

Infectious Bronchitis (IB) is an acute, highly contagious viral respiratory disease of chicken’s caracterized by tracheal rallies, coughing, sneezing and nasal discharge in young chicks. In addition, the disease may affect kidhney, and in laying flock there is usually a drop in egg production and quality. IB is a major negative economic importance in poultry industry because the disease causes poor weight gain and feed efficiency, mortality in young chicks, reduction in egg production and egg quality in laying flock. IB is distributed worldwide and has been reported to be present in Indonesia. IB is caused by virus of a member of Coronaviridae under genera of Coronavirus. Spreading of IB virus among chickens usually by inhalation. Diagnosis of the disease can be based on the isolation and identification of the virus using embryonated chicken eggs and trachea organ culture. There is no treatment available for IB, so the control of the disease is mainly by vaccination. The existence of multiple serotipes of IB virus requires vaccines which are represent the antigenic spectrum of field isolates. To ensure the results of vaccination program, monitoring antibody titers following vaccination is recommended. The most widely used serological test for antibody monitoring is an enzyme linked immunosorbent assay (ELISA) or Haemaglutination Inhibition (HI) test.   Key words: IB, virus, chicken, control
IDENTIFIKASI MOLEKULER VIRUS SUBTIPE H3 DAN H10 PADA UNGGAS Dharmayanti, Indi; Indriani, Risa
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (162.099 KB) | DOI: 10.21157/j.ked.hewan.v8i1.1249

Abstract

Penelitian ini bertujuan mengidentifikasi molekuler virus-virus influenza yang tidak dapat diidentifikasi dengan primer H5N1 sehingga perlu dilakukan identifikasi lanjutan dengan primer virus influenza lainnya untuk mengetahui jenis subtipe virus influenza yang bersirkulasi di lapang. Sampel yang digunakan adalah lima sampel cairan alantois (Kode B1-B5) yang diduga mengandung virus influenza selain virus H5N1. Metode yang digunakan pada penelitian ini adalah dengan reverse transcription polymerase chain reaction (RT-PCR) menggunakan beberapa set primer influenza dan konfirmasi dilakukan dengan pengurutan deoxyribonucleic acid (DNA). Dari hasil analisis RT-PCR dan pengurutan DNA menunjukkan bahwa sampel dengan kode B1 dan B2 menunjukkan homologi tertinggi dengan data virus yang terdaftar di GenBank (NCBI) yaitu dengan virus influenza subtipe H3 sedangkan sampel dengan kode B4 mempunyai homologi tertinggi dengan virus influenza subtipe H10. Dari identifikasi dan karakterisasi tersebut kemungkinan bahwa virus sampel kode B1 (A/Chicken/Buleleng/BBVD488-9/2009) dan (B2) A/Duck/Tabanan/BBVD573-10/2009 adalah virus (AI) subtipe H3 sedangkan sampel kode B4 (A/Chicken/Klungkung/ BBVD006-1/2010) adalah virus AI subtipe H10.
Studi Efikasi Vaksin Bivalen AI Isolat Lokal terhadap Beberapa Karakter Genetik Virus AI subtipe H5N1 Indriani, Risa; Dharmayanti, NLP Indi
JURNAL BIOLOGI INDONESIA Vol 9, No 1 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.942 KB) | DOI: 10.14203/jbi.v9i1.143

Abstract

Studi vaksin inaktif bivalen AI isolat lokal subtipe H5N1 terhadap beberapa karakter genetik virus AI H5N1 padaayam layer dan broiler. Vaksin inaktif bivalen dari isolat lokal AI A/Ck/west java/Smi-M6/2008 and A/Ck/westjava/Pwt-D10-39/2010. Ayam layer dan broiler komersial divaksinasi dengan vaksin inaktif bivalen AI isolat lokal,setelah 3 minggu vaksinasi ditantang dengan virus AI A/Ck/west java/Smi-Part/2006, A/Ck/west java/Subang-JAPFA-29/2007 and A /Ck/west java/Smi-Rahm2/2011. Ayam layer vaksinasi mendapat perlindungan dari morbiditas,mortalitas dan penurunan ekskresi virus tantang dengan tingkat proteksi 90-100% sedangkan ayam layerkontrol mati dalam waktu 2-3 hari, sementara broiler yang divaksinasi tidak mendapatkan perlindungan dari morbiditasdan mortalitas setelah terinfeksi virus AI tantang. Hasil studi memperlihatkan vaksin inaktif bivalen AI isolatlokal subtipe H5N1 mampu memberikan perlindungan pada ayam layer dari infeksi beberapa karakter genetikvirus AI subtipe H5N1 .Kata kunci: Vaksin bivalen, subtipe H5N1, tantang dan proteksi
Infectious Bronchitis (IB) Disease and its Control in Chicken Indriani, Risa; ., Darminto
Indonesian Bulletin of Animal and Veterinary Sciences Vol 9, No 2 (1999)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.556 KB) | DOI: 10.14334/wartazoa.v9i2.723

Abstract

Infectious Bronchitis (IB) is an acute, highly contagious viral respiratory disease of chicken’s caracterized by tracheal rallies, coughing, sneezing and nasal discharge in young chicks. In addition, the disease may affect kidhney, and in laying flock there is usually a drop in egg production and quality. IB is a major negative economic importance in poultry industry because the disease causes poor weight gain and feed efficiency, mortality in young chicks, reduction in egg production and egg quality in laying flock. IB is distributed worldwide and has been reported to be present in Indonesia. IB is caused by virus of a member of Coronaviridae under genera of Coronavirus. Spreading of IB virus among chickens usually by inhalation. Diagnosis of the disease can be based on the isolation and identification of the virus using embryonated chicken eggs and trachea organ culture. There is no treatment available for IB, so the control of the disease is mainly by vaccination. The existence of multiple serotipes of IB virus requires vaccines which are represent the antigenic spectrum of field isolates. To ensure the results of vaccination program, monitoring antibody titers following vaccination is recommended. The most widely used serological test for antibody monitoring is an enzyme linked immunosorbent assay (ELISA) or Haemaglutination Inhibition (HI) test.   Key words: IB, virus, chicken, control
The isolation of Gurnbiro virus from larvae and darkling Ivelles (Carcinops pumilin) Parede, Lies; Indriani, Risa; ., Sukarsih
Indonesian Journal of Animal and Veterinary Sciences Vol 2, No 1 (1996)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (472.359 KB) | DOI: 10.14334/jitv.v2i1.42

Abstract

Gumboro (infectious bursal disease, IBD) virus was isolated from darkling beetles (Carrinaps pumilin) and their larvae in a commercial pulletchicken farm with repeated outbreaks incidence of Gumboro disease in Tangertng, West Java. In addition, these over populated beetles and their larvae were suspected to be infected and then shed the virus or acted as vectors. Isolation was done by repeated passages of virus using chicken embryo fibroblast cells as prime media, which then showed the evidence of cylop: ihic effecis. The isolation was followed by antigen detection by means of ELISA test.   Key words: Gumboro disease, infectious bursal disease, darkling beetle, Carcinops punulin  
Serotype variation among infectious bronchitis viral isolates taken from several areas of Java Indriani, Risa; ., Darminto
Indonesian Journal of Animal and Veterinary Sciences Vol 5, No 4 (2000)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.347 KB) | DOI: 10.14334/jitv.v5i4.188

Abstract

Infectious bronchitis (IB) is an acute highly contagious viral respiratory disease of poultry caused by virus belongs to the family of Coronaviridae. The virus consist of many serotypes with low level of cross-protectivity among serotypes. Field data showed that the outbreaks of IB were frequently reported in chicken flocks, although vaccinations against the disease have been practiced. Hence, the study on serotype relationship among isolates of the viruses is essentially required. The aim of this study was to isolate and characterize IB viruses from chicken flocks in some areas of Java. Isolation of the virus was carried out in nine-day old embrionated chicken eggs and identified by means of agar gel precipitation (AGP) tests against standard antisera to IB virus. The serotypes of the IB viral isolates were determined by cross-neutralization tests in nine day old embryonated chicken eggs using r value derived from homologous and heterologous serum titres as criteria. This study obtained 12 IB viral isolates which were identified on the basis of the ability to cause lesions in chicken embryos and positive to agar gel presipitation test against standard positive antiserum to the virus. Based on the cross-neutralization tests in embryonated chicken eggs, isolate I.9 was formed to have relationship closed to Mass-41 serotype, while I.2, I. 3, and I.7 isolates were closely to the serotype of Con-46. Virus isolates (I.5, I.14, I.24, and I.25) were decided to have no serotype relationships to either Mass-41 or Con-46 serotype. Since the I.5, I.14, I.24 and I.25 isolates were not neutralized by antisera against the previous identified local infectious bronchitis viral isolates, and that were considered to be distinct serotype to the previously identified local IB viral isolates.   Key words: Infectious bronchitis, virus, embryonated egg, cross neutralization test.
Antibody response and protection of inactivated-local isolate vaccine for infectious bronchitis in laying chicken Indriani, Risa; ., Darminto
Indonesian Journal of Animal and Veterinary Sciences Vol 6, No 2 (2001)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (53.569 KB) | DOI: 10.14334/jitv.v6i2.230

Abstract

Infectious bronchitis (IB) is an acute highly contagious viral respiratory disease of poultry caused by Coronavirus. IBV infection consists of many serotypes and can only be controlled by vaccination. An effective IB vaccine should be prepared from local isolates, due to the antigenic variation among serotypes. The aims of this research were to develop inactivated IB vaccine derived from IBV local isolate and to determine the efficacy of that vaccine in layer flocks. Five layer chicken groups were used in this experiments, group I was vaccinated with commercial IBV live vaccine thrice, group II was vaccinated with commercial IBV live vaccine once and repeated with inactivated local IBV isolate twice, group III was vaccinated with commercial IBV live vaccine once and repeated with commercial inactivated twice, group IV was vaccinated with IBV live vaccine once, and group V was not vaccinated. After the chickens reached at a stable egg production they were challenged with IBV local isolates. Antibody responses were examined by means of haemagglutination hibitition (HI) test and HI titres were expressed as log2 of the reciprocal of the highest dilution of serum causing inhibition of a log2 HA titre of 2. The mean titres of antibody responses of chicken in group I, II, III, IV, and V was 4.9 ± 0.87, 6.8 ± 0.97, 7.7 ± 0.46, 2.9 ± 0.94, and 2.0 ± 1.67 respectively. The levels of protection against challenges were determined by viral isolation, this in group I, II, III, IV, and V was 63, 73, 60, 50, and 0% respectively. Clinical symptom of egg quality was slightly reduced in group I, IV, and V and it were unchanged in group II and III. Group II gave better in number of egg  production than the other groups. The results indicated that the IBV inactivated localisolate vaccine gave high titres of  antibody and higher protection rates than that of commercial IBV inactivated vaccine. Inaddition, IBV local isolate vaccinated group prevented from declining egg production after challenged with IBV local isolate.   Key words: Infectious bronchitis, layer, antibody titre, vaccine, challenge virus
Antibody response and protection of inactivated-local isolate vaccine for infectious bronchitis in laying chicken Indriani, Risa; ., Darminto
Indonesian Journal of Animal and Veterinary Sciences Vol 6, No 2 (2001)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (161.334 KB) | DOI: 10.14334/jitv.v6i2.231

Abstract

Infectious bronchitis (IB) is an acute highly contagious viral respiratory disease of poultry caused by Coronavirus. IBV infection consists of many serotypes and can only be controlled by vaccination. An effective IB vaccine should be prepared from local isolates, due to the antigenic variation among serotypes. The aims of this research were to develop inactivated IB vaccine derived from IBV local isolate and to determine the efficacy of that vaccine in layer flocks. Five layer chicken groups were used in this experiments, group I was vaccinated with commercial IBV live vaccine thrice, group II was vaccinated with commercial IBV live vaccine once and repeated with inactivated local IBV isolate twice, group III was vaccinated with commercial IBV live vaccine once and repeated with commercial inactivated twice, group IV was vaccinated with IBV live vaccine once, and group V was not vaccinated. After the chickens reached at a stable egg production they were challenged with IBV local isolates. Antibody responses were examined by means of haemagglutination hibitition (HI) test and HI titres were expressed as log2 of the reciprocal of the highest dilution of serum causing inhibition of a log2 HA titre of 2. The mean titres of antibody responses of chicken in group I, II, III, IV, and V was 4.9 ± 0.87, 6.8 ± 0.97, 7.7 ± 0.46, 2.9 ± 0.94, and 2.0 ± 1.67 respectively. The levels of protection against challenges were determined by viral isolation, this in group I, II, III, IV, and V was 63, 73, 60, 50, and 0% respectively. Clinical symptom of egg quality was slightly reduced in group I, IV, and V and it were unchanged in group II and III. Group II gave better in number of egg  production than the other groups. The results indicated that the IBV inactivated localisolate vaccine gave high titres of  antibody and higher protection rates than that of commercial IBV inactivated vaccine. Inaddition, IBV local isolate vaccinated group prevented from declining egg production after challenged with IBV local isolate.   Key words: Infectious bronchitis, layer, antibody titre, vaccine, challenge virus
Comparison of sequences of hypervariable region (HVR) subunit S-1 gene of field isolate I-37 infectious bronchitis virus with Connecticut serotype Dharmayanti, N.L.P Indi; Indriani, Risa; ., Darminto
Indonesian Journal of Animal and Veterinary Sciences Vol 8, No 2 (2003)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (177.798 KB) | DOI: 10.14334/jitv.v8i2.380

Abstract

Infectious Bronchitis is a contagious and acute respiratory disease in chickens caused by infectious bronchitis virus (IBV).Antigenic differences in IBV are associated with changes in the sequence of the spike glycoprotein (S). The subunit S1 which demonstrates more sequence variability than S-2 have been identified as hypervariable region (HVR-1 and 2). There were several IB virus field isolates included I-37 have been identified in Indonesia by serum neutralization method. However, gene sequence variation in HVR subunit S-1 had not yet been identified. Isolate I-37 was close to the serotype Connecticut 46 (Conn 46). The aim of this study is to identify sequence variation of HVR subunit S-1 gene of isolate I-37 produced by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and sequencing. Several procedures were carried out in the study including virus titration, propagation and was concentrated from the allantoic fluid infected with IBV. Then, RNA was extracted for RTPCR. urther the product was sequnced and its homology with IBV references from GenBank was compared by GenMac version 8.0. Result showed that isolate I-37 produced 515 bp of amplification product. Isolate I-37 and Conn 46 are same serotype, yet their HVR subunit S-1 nucleotides and amino acids (protein) differ by 6.9% and 15.6% respectively. It might be concluded that isolate I-37 was variant of Conn 46.   Key words: Sequences variation, IBV, I-37 field isolate, HVR subunit S-1 gene
Prototype of A/Duck/Sukoharjo/Bbvw-1428-9/2012 subtipe H5N1 clade 2.3.2 as vaccine on local duck Indriani, Risa; Dharmayanti, NLPI
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 2 (2014)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.25 KB) | DOI: 10.14334/jitv.v19i2.1044

Abstract

A/Duck/Sukoharjo/Bbvw-1428-9/2012 virus subtipe H5N1 clade 2.3.2 as seed vaccine on local duck. AI H5N1 clade 2.3.2 vaccine containing 256 HAU per dose was formulated using adjuvant ISA 71VG Montanide ™. Six groups of one day old local duck were used in this study. Three groups (10 ducks per group) were vaccinated and 3 groups (9 duck per group) were served control. Vaccination was conducted when the duck were three weeks old of age using single dose. Three weeks after vaccination when the duck were challenged either with HPAI H5N1 clade 2.3.2, or HPAI H5N1 clade 2.1.3 virus at dose 106 EID50/ 0.1 ml by drops  intranasaly. Result showed that vaccination produced 100% protection compared to unvaccinated ducks againt HPAI subtipe H5N1 clade 2.3.2, and 100% protection againt HPAI H5N1 clade 2.1.3 (A/ck/wj/Subang-29/2007 and A/ck/wj/Smi-Part/2006), while unvaccinated ducks showed virus shedding on day 3 post infection. Key Words: Duck, Influenza, Clade 2.3.2, Vaccine, Clade 2.1.3