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PENGARUH UKURAN SERBUK DAN PENAMBAHAN TEMPURUNG KELAPA TERHADAP KUALITAS PELET KAYU SENGON Hasna, Anindya Husnul; Sutapa, J. P. Gentur; Irawati, Denny
Jurnal Ilmu Kehutanan Vol 13, No 2 (2019)
Publisher : Fakultas Kehutanan Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.009 KB) | DOI: 10.22146/jik.52428

Abstract

Limbah industri kayu sengon menjadi salah satu bahan baku dalam pembuatan pelet kayu karena potensinya yang cukup besar. Akan tetapi pelet kayu sengon memiliki kerapatan serta nilai kalor yang rendah. Untuk meningkatkan sifat bahan bakar pelet kayu Sengon maka dilakukan pencampuran bahan dengan serbuk tempurung kelapa. Penelitian ini menggunakan bahan dari limbah serbuk gergaji sengon (Falcataria moluccana (Miq.)) dan limbah tempurung kelapa (Cocos nucifera). Masing-masing bahan dibuat partikel pada 3 kelompok ukuran yaitu 20-40 mesh, 40-60 mesh, dan 60-80 mesh. Ke dalam serbuk kayu sengon ditambahkan serbuk tempurung kelapa dengan penambahan 25%, 50%, dan 75%, sedangkan untuk kontrol (0%) adalah pelet kayu sengon tanpa penambahan tempurung kelapa. Pelet dibuat dengan menggunakan single-pelletizer pada suhu ruang dengan tekanan 100 kg/cm2. Hasil penelitian menunjukkan kombinasi bahan baku yang berbeda (sengon dan tempurung kelapa) memberikan pengaruh terhadap sifat fisika dan kimia pelet kayu. Semakin tinggi persentase campuran serbuk tempurung kelapa pada pelet kayu sengon maka semakin tinggi keteguhan tekan, karbon terikat, total karbon dan nilai kalor, sedangkan untuk kadar zat mudah menguap, kadar abu, kadar N, S, dan H semakin rendah. Pelet terbaik dihasilkan pada kombinasi penambahan tempurung kelapa 50% dengan ukuran 60-80 mesh yang memiliki sifat kadar abu yang rendah (0,79%) dan nilai kalor yang tinggi (5129,07 Kal/g), serta keteguhan tekan yang masih cukup tinggi (444,75N). Hasil tersebut memenuhi standar SNI 8021:2014.Effect of Particle Size and Addition of Coconut Cell on the Quality of Sengon Wood PelletAbstractThe waste of sengon (Falcataria moluccana) industry becomes one of the raw materials in the manufactured of wood pellets, because of its potency. However F. moluccana pellets posses low density and calorific value. To improve its properties, a materials mixing with coconut shell parcticles was conducted. This study used material from the waste of sengon (F. moluccana) sawdust and the waste of coconut (Cocos nucifera). Particles from those materials were made on 3 sizes which are 20-40 mesh, 40-60 mesh, and 60-80 mesh. 25%, 50%, and 75% of coconut shell were added into sengon sawdust, while woode pellets with no additions were used as a control. Pellets are made using single-pelletizer at room temperature with a pressure of 100 kg/cm2. The research results showed if the different material combination (sengon and coconut shell) gave significant effect to physical properties and chemical content of wood pellets. Higher percentage of coconut shell gives higher compressive strength, fixed carbon content, total of carbon, and calorific value, while volatile matter, ash content, N, S, and H content showed lower value. The best pellet was resulted from combination between coconut shell addition 50% and nesh size 60 ? 80 which posses quite low ash content (0.79%) and high calorific value (5129.07 Kal/g), and high compression strength (444.75 N). This result has qualified the standard of SNI 8021:2014.
OPTIMASI PRODUKSI BADAN BUAH TIGA JENIS JAMUR KAYU DENGAN INOVASI PERLAKUAN PADA WAKTU INKUBASI DAN JUMLAH PENYOBEKAN PADA BAGLOG Irawati, Denny; P, Naresvara Nircela P Nircela; RM, Febe Margareta; Sutapa, J.P. Gentur Sutapa Gentur
Jurnal Ilmu Kehutanan Vol 13, No 1 (2019)
Publisher : Fakultas Kehutanan Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (486.162 KB) | DOI: 10.22146/jik.46209

Abstract

Di Indonesia permintaan jamur konsumsi, baik yang untuk obat maupun bahan makanan, terus meningkat. Akselerasi produksi perlu dilakukan untuk memenuhi kebutuhan pasar. Penelitian ini bertujuan untuk mengetahui pengaruh lama waktu inkubasi dan banyaknya jumlah penyobekan baglog terhadap produktivitas 3 jenis jamur konsumsi yang banyak dibudidayakan di Indonesia. Sampel baglog pada penelitian ini diperoleh dari petani jamur Sedyo Lestari, Bantul. Selanjutnya pada baglog tersebut diinokulasikan 3 jenis jamur yaitu Auricularia sp. (jamur kuping), Pleurotus sp. (jamur tiram), dan Ganoderma sp. (jamur lingzhi). Setelah inokulasi, media diinkubasi selama 30, 40, dan 50 hari, untuk selanjutnya dibudidayakan selama 60 hari. Pada akhir masa inkubasi dilakukan pengukuran kadar glukosamin dan penyobekan baglog pada 1 atau 2 ujung untuk memicu munculnya badan buah. Selama periode pembudidayaan, dilakukan pemanenan badan buah dan diukur produktivitas badan buah serta intensitas pemanenan. Hasil penelitian menunjukkan bahwa perlakuan lama waktu inkubasi dan jumlah sobekan pada baglog memberikan pengaruh yang berbeda terhadap setiap jenis jamur. Lama waktu inkubasi tidak berpengaruh nyata terhadap produktivitas badan buah dan nilai konversi biologi pada jamur tiram dan kuping, namun berpengaruh nyata terhadap jamur lingzhi. Lama waktu inkubasi terbaik untuk jamur lingzhi adalah 40 hari. Jumlah sobekan pada baglog tidak memberi pengaruh terhadap produktivitas jamur tiram dan kuping, akan tetapi berpengaruh terhadap produktivitas jamur lingzhi dan intensitas pemanenan jamur kuping.  Optimization of Fruiting Body Production of Three Kinds Edible Mushrooms Species by Innovate the Incubation Time and Number of Rips on Baglog AbstractIn Indonesia the demand for edible mushroom, both for medicine and food, continues to increase. Production acceleration is needed to meet the market needs. This study aims to determine the effect of the incubation time and the number of rips on baglog to the productivity of 3 species of edible mushrooms that are widely cultivated in Indonesia. The baglog as sample in this study was obtained from Sedyo Lestari mushroom farmer in Bantul. The baglog was inoculated by 3 kinds of mushroom of Auricularia sp. (ear fungus), Pleurotus sp. (oyster mushroom), and Ganoderma sp. (lingzhi mushroom). After inoculation, the medium was incubated for 30, 40, and 50 days, for subsequent cultivation for 60 days. At the end of the incubation period, the glucosamine content was analysed and the baglog was teared at 1 or 2 ends to trigger the appearance of the fruiting body. During the cultivation period, the fruiting bodies were harvested and the productivity of the fruiting body and the harvesting intensity were measured. The results showed that the incubation time and the amount of rips on the baglog gave a different effect on each mushroom species. The duration of incubation time had no significant effect on fruiting body productivity and biological conversion on oyster and ear mushrooms.However, it had a significant effect on Lingzhi mushroom. The best time of incubation for Lingzhi mushroom was 40 days. The amount of rips on the baglog did not give effect to the productivity of oyster and ear mushrooms, but it affected the productivity of Lingzhi mushroom and the harvesting intensity of ear mushroom.
HIDROLISIS MEDIA SISA BUDIDAYA JAMUR KUPING MENGGUNAKAN TIGA JENIS ENZIM SELULASE Irawati, Denny
Jurnal Ilmu Kehutanan Vol 11, No 1 (2017)
Publisher : Fakultas Kehutanan Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (453.591 KB) | DOI: 10.22146/jik.24900

Abstract

Jamur kuping (Auricularia polytricha) termasuk dalam kelas Basidiomycetes yang banyak dibudidayakan di Indonesia. Setelah 6-8 bulan masa pembudidayaan media budidaya jamur kuping harus diperbaharui. Sejauh ini media sisa budidaya jamur kuping belum dimanfaatkan dengan baik, bahkan limbah tersebut hanya dibuang begitu saja sebagai limbah. Hal ini sangat berbahaya karena sisa miselia yang terdapat di dalam media sisa dapat mendegradasi lebih lanjut media dan mengakibatkan pencemaran lingkungan yaitu terlepasnya gas metana ke udara. Oleh karena itu pemanfaatan sisa media budidaya kuping perlu dilakukan antara lain untuk memproduksi gula pereduksi dengan menggunakan berbagai jenis enzim selulase. Penelitian ini menggunakan media sisa budidaya jamur kuping yang berasal dari 3 jenis kayu, yaitu sengon (Falcataria moluccana), jati (Tectona grandis), dan meranti (Shorea sp.), yang dihidrolisis menggunakan 3 jenis enzim selulase yaitu Driselase, Cellulase ?Onozuka? R-10, dan Meicelase. Setelah hidrolisis, kemudian diukur laju hidrolisisnya dan dianalisis kadar gula pereduksinya. Rancangan yang digunakan adalah rancangan acak lengkap dengan aras 3 x 3 serta ulangan sebanyak 3 untuk setiap perlakuan. Hasil penelitian menunjukkan bahwa media sisa budidaya jamur kuping memiliki potensi sebagai bahan baku gula pereduksi yang selanjutnya dapat difermentasi menjadi bio-etanol atau bahan kimia lainnya. Laju hidrolisis berkisar antara 1,43-21,29%, dengan kombinasi tertinggi yaitu media sisa budidaya yang terbuat dari serbuk kayu meranti dengan menggunakan enzim Meicelase. Kadar dan rendemen gula pereduksi tertinggi juga dihasilkan dari kombinasi perlakuan yang sama, yaitu berturut-turut sebesar 127,7 mg/g dan 12,8% dengan kisaran hasil antara 56,01-127,7 mg/g atau rendemen 5,6-12,8%.The Hydrolysis of Ear-Mushroom Cultivation Media Residue by Using Three Kinds of Cellulase EnzymesAbstractEar mushrooms (Auricularia polytricha) belongs to class Basidiomycetes is widely cultivated in Indonesia. After 6-8 months of cultivation, the media should be renewed. Therefore, the rest of the media is under utilized and just thrown away as a waste. This is dangerous because the residual mycelia that contained in the rest of the media may further degrade the media. This pollutes the environmental pollution due to discharge of methane into the air. Therefore, the utilization of residual ear mushroom media needs to be processed. In fact, this residue can produce a reducing-sugar using various commercial cellulase enzymes. This study used the residual media of the cultivation of mushroom derived from three types of wood, i.e. sengon (Falcataria moluccana), teak (Tectona grandis), and meranti (Shorea sp.), and then they were hydrolyzed using three types of cellulase enzymes, i.e. Driselase, Cellulase ?Onozuka? R-10, and Meicelase. The hydrolysis rate was measured and the reducing-sugar yield was analyzed. The design used was completely randomized design with 3 x 3 factors and 3 replications for each treatment. The results showed that the residue of the mushroom cultivation media has the potential of reducing sugar as a raw material which can then be fermented into bio-ethanol or other chemicals. Hydrolysis rate ranged from 1.43 to 21.29%. The highest combination of residual cultivation medium was made from meranti sawdust by using Meicelase enzyme. The highest reducing sugar content yield were also resulted from the combination of the same treatment, which amounted to 127.7 mg/g or 12.8% with output range between 56.0 and 127.7 mg/g (or yield 5.6-12.8%).
Usaha Pemanfaatan Limbah Budidaya Jamur Sebagai Bahan Baku Pembuatan Briket Untuk Energi di Kelompok Tani Jamur Sedyo Lestari Irawati, Denny; Pradipta, Naresvara Nircela; Sutapa, Johanes Pramana Gentur
Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 2, No 2 (2017): Maret
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (414.788 KB) | DOI: 10.22146/jpkm.27033

Abstract

Mushroom farmer society Sedyo Lestari is located in the Argosari, Sedayu, Bantul. The high demand for mushrooms media (baglog) is a good business opportunities for this farmer society. But the process of baglog making requires a lot of energy in the form of firewood, especially for sterilization process of the baglog. On the other hand, the mushroom cultivation process itself remains the waste that can be used as fuel for the sterilization process. So far Sedyo Lestari farmer society members do not have the technology to used the mushroom cultivation waste as a fuel. Therefore, it is imperative to transfer knowledge of biomass briquetting technique. The method used in this activities are socialization, training, and mentoring by starting with the introduction of briquettes and briquetting technology. Subsequently made briquette-making machines followed by training briquette making. Mentoring and socialization were also made to all members of farmer society. Outcome of these activities was briquette-making machines that can be used by all members of the society as well as knowledge about the process of making briquettes. The benefit of using briquettes of mushroom cultivation media waste as fuel for subtitute the firewood can provide savings to the expenditures of farmers for buying firewood and solving the waste management problems for farmers.
Optimalisasi Penggunaan Lahan dengan Sistem Agroforestri dan Pendampingan Pascapanennya di Kelompok Tani Dusun Kemuning, Gunungkidul Kusumandari, Ambar; Irawati, Denny; Soedjoko, Sri Astuti
Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 1, No 1 (2015): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpkm.16924

Abstract

Kemuning sub village district is located in Bunder village, Patuk, Gunung Kidul. This area is near by the Bunder forest park and Wanagama Educational Forest. Most of the people live there are farmers. As the soil is relatively unfertile, they prefer to plant trees, so they called as forest community farmers. Among the trees, they plant cassava, peanut, and corn, so it called as agroforestry. In the field, the area seems still has the opportunity that can be added to be planted. Based on this situation, in this project, the optimalization of land use was introduced to ask the farmers to plant the undergrowth species includes: zingiber, kunyit and kencur. This project was started by introducing the agroforestry system, where they can plant together both trees and non trees at the same land. The socialization was done by inviting the farmers to come to the meeting. The advantages of agroforestry was explained to them, and also the post harvesting management to produce the instant zingiber was also trained and demonstrated to the female farmers. This product is also useful for attracting the local tourist who usually come at the week end. The output of this project was the agroforestry demplot can be built in the field, so the farmers can learn and study to develope at their own land. The female farmer can also make the instant zingiber as the healthy drink.
Usaha Pemanfaatan Limbah Budidaya Jamur Sebagai Bahan Baku Pembuatan Briket Untuk Energi di Kelompok Tani Jamur Sedyo Lestari Irawati, Denny; Pradipta, Naresvara Nircela; Sutapa, Johanes Pramana Gentur
Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 2, No 2 (2017): Maret
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpkm.27033

Abstract

Mushroom farmer society Sedyo Lestari is located in the Argosari, Sedayu, Bantul. The high demand for mushrooms media (baglog) is a good business opportunities for this farmer society. But the process of baglog making requires a lot of energy in the form of firewood, especially for sterilization process of the baglog. On the other hand, the mushroom cultivation process itself remains the waste that can be used as fuel for the sterilization process. So far Sedyo Lestari farmer society members do not have the technology to used the mushroom cultivation waste as a fuel. Therefore, it is imperative to transfer knowledge of biomass briquetting technique. The method used in this activities are socialization, training, and mentoring by starting with the introduction of briquettes and briquetting technology. Subsequently made briquette-making machines followed by training briquette making. Mentoring and socialization were also made to all members of farmer society. Outcome of these activities was briquette-making machines that can be used by all members of the society as well as knowledge about the process of making briquettes. The benefit of using briquettes of mushroom cultivation media waste as fuel for subtitute the firewood can provide savings to the expenditures of farmers for buying firewood and solving the waste management problems for farmers.
Optimalisasi Penggunaan Lahan dengan Sistem Agroforestri dan Pendampingan Pascapanennya di Kelompok Tani Dusun Kemuning, Gunungkidul Kusumandari, Ambar; Irawati, Denny; Soedjoko, Sri Astuti
Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 1, No 1 (2015): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (477.669 KB) | DOI: 10.22146/jpkm.16924

Abstract

Kemuning sub village district is located in Bunder village, Patuk, Gunung Kidul. This area is near by the Bunder forest park and Wanagama Educational Forest. Most of the people live there are farmers. As the soil is relatively unfertile, they prefer to plant trees, so they called as forest community farmers. Among the trees, they plant cassava, peanut, and corn, so it called as agroforestry. In the field, the area seems still has the opportunity that can be added to be planted. Based on this situation, in this project, the optimalization of land use was introduced to ask the farmers to plant the undergrowth species includes: zingiber, kunyit and kencur. This project was started by introducing the agroforestry system, where they can plant together both trees and non trees at the same land. The socialization was done by inviting the farmers to come to the meeting. The advantages of agroforestry was explained to them, and also the post harvesting management to produce the instant zingiber was also trained and demonstrated to the female farmers. This product is also useful for attracting the local tourist who usually come at the week end. The output of this project was the agroforestry demplot can be built in the field, so the farmers can learn and study to develope at their own land. The female farmer can also make the instant zingiber as the healthy drink.
PEMANFAATAN SERBUK KAYU UNTUK PRODUKSI ETANOL DENGAN PERLAKUAN PENDAHULUAN DELIGNIFIKASI MENGGUNAKAN JAMUR PHANEROCHAETE CHRYSOSPORIUM Irawati, Denny; Azwar, Norman Razief; Syafii, Wasrin; Artika, I Made
Jurnal Ilmu Kehutanan Vol 3, No 1 (2009)
Publisher : Fakultas Kehutanan Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13163.557 KB) | DOI: 10.22146/jik.925

Abstract

Utilization of Sawdust to Produce Ethanol Using Delignification Pre-treatment with White Rot Fungi Phanerochaete chrysosporiumCurrently, Indonesia is in the middle ofpetroleum crisis. One ofthe alternative fuels which can be used as a petroleum substitute is ethanol. Ethanol can be produced from timber waste (sawdust). Indonesia in 2003 had timber waste potency of about 3-4 millions m3. However, ethanol production from sawdust has problems due to its lignin content. Therefore, research on bio-delignification treatment of sawdust prior to ethanol making process is required. In the present study ethanol was produced by simultaneous saccharification and fermentation (SSF) using crude cellulose from Trichoderma viride and Saccharomyces cerevisiae. The raw materials for ethanol production are sengon (Paraserianthes falcataria (L.) Nielsen syn.), meranti (Shorea sp.) and teak (Tectona grandis LIIVN.f.) sawdust after pretreatment with white rot fungi Phanerochaete chrysosporium for 10, 20 and 30 days incubation time. The yield of ethanol was between 1.65-44.83 g/1. The best combination treatment is sengon sawdust with 30 day incubation time.
ASSOCIATION OF MUSHROOM CULTIVATION AND OZONOLYSIS AS PRETREATMENT FOR ENZYMATIC SACCHARIFICATION OF SENGON(FALCATARIA MOLUCCANA) SAWDUST Irawati, Denny; Wedatama, Soekmana; Ishiguri, Futoshi; Yokota, Shinso
Jurnal Ilmu Kehutanan Vol 12, No 1 (2018)
Publisher : Fakultas Kehutanan Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (368.323 KB) | DOI: 10.22146/jik.34104

Abstract

Wood industry based on sengon (Falcataria moluccana) material has developed rapidly and cause the huge wastes. Lignocellulosic materials, such as sengon sawdust, offer a great potential as cheap and abundant feedstock for biofuels production. Ozone oxidation pretreatment was carried out on sengon wastes to improve fermentable sugar production by enzymatic saccharification. Sengon wood (SW), sengon media (SM), and sengon spent media (SMM), that was waste of media after mushroom cultivation, treated with ozone for different treatment time were enzymatically saccharified. Then the decrease of hydrolysis weight, reducing sugar yield, and monosaccharide yields were determined. Ozone treatment resulted in Klason lignin degradation in the all samples, resulting in the improvement of subsequent enzymatic saccharification. Ozone treatment with the duration more than 30 min was not suitable for SMM sample. Glucose yield after saccharification from the ozone-treated sample for 60 min was the highest among all samples in SW and SM treated, but for SMM it was 30 min. Based on these results, we concluded that mushroom cultivation can reduce the ozone time treatment and it is an effective treatment to improve sugar yield by enzymatic saccharification of sengon wastes. Gabungan Pra-perlakuan Ozonolisis dan Budidaya Jamur Sebelum Sakarifikasi Enzimatis Serbuk Kayu Sengon (Falcataria moluccana)IntisariIndustri kayu berbahan baku kayu sengon (Falcataria moluccana) saat ini tengah berkembang dengan sangat pesat dan hal ini menyebabkan timbulnya banyak limbah kayu sengon. Materi lignosellulosa, seperti limbah serbuk kayu sengon, merupakan potensi yang sangat baik sebagai bahan baku bio-fuel karena murah dan banyak tersedia. Praperlakuan dengan oksidasi ozon pada limbah kayu sengon dilakukan untuk meningkatkan produksi gula reduksi melalui proses sakarifikasi enzimatis. Serbuk sengon (SW), media sengon (SM), dan limbah media sengon (SMM), yang merupakan sisa dari media budidaya jamur, diberi perlakuan dengan ozon pada berbagai lama waktu perlakuan. Sampel yang sudah diberi perlakuan ozon tersebut kemudian dianalisis kandungan kimianya dan di-sakarifikasi secara enzimatis. Selanjutnya diukur laju hidrolisis, kadar gula pereduksi, dan kadar monosakarida. Perlakuan dengan ozon menyebabkan degradasi kadar Klason lignin di semua sampel, sehingga mengakibatkan peningkatan kemampuan sakarifikasi dari enzim. Perlakuan ozon dengan lama waktu lebih dari 30 menit tidak sesuai untuk sampel SMM. Kadar glukosa hasil sakarifikasi yang tinggi diperoleh dari sampel SW dan SM yang diberi perlakuan ozon selama 60 menit, serta SMM yang diberi perlakuan 30 menit. Berdasarkan hasil ini, dapat disimpulkan bahwa perlakuan jamur dapat mengurangi lama waktu perlakuan ozon dan praperlakuan efektif untuk meningkatkan kadar gula reduksi yang dihasilkan dari sakarifikasi enzimatis limbah kayu sengon.
PENGGUNAAN Phanerochaete chrysosporium PADA PENGOLAHAN PULP BIO-SEMI-MEKANIS KAYU TERENTANG Aprianis, Yeni; Irawati, Denny; Marsoem, Sri Nugroho
Jurnal Penelitian Hasil Hutan Vol 34, No 3 (2016): Jurnal Penelitian Hasil Hutan
Publisher : Pusat Penelitian dan Pengembangan Hasil Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jphh.2016.34.3.231-239

Abstract

Kayu terentang (Campnosperma auriculata Hook.f) memiliki berat jenis rendah (0,3), sehingga salah satu alternatif pemanfaatannya menjadi pulp adalah dengan proses semi-mekanis. Pengurangan konsumsi energi pada proses kimia-mekanis dilakukan menggunakan jamur pendegradasi lignin (Phanerochaete chrysosporium) sebagai perlakuan awal. Pengolahan pulp semi-mekanis menggunakan NaOH 4% dan masa inkubasi 0 (kontrol), 3, 4, dan 5 minggu. Hasil penelitian menunjukkan bahwa perlakuan P. chrysosporium berpengaruh nyata terhadap kehilangan berat, kandungan kimia, dan kebutuhan energi refining. Kehilangan berat serpih kayu berkisar 15,95-21,31% dan peningkatan kadar selulosa mencapai 6,77%. Inkubasi selama lima minggu menurunkan kadar lignin hingga 22,97% dan menghemat energi refining sebesar 22,7%.