Mayta Novaliza Isda
Jurusan Biologi, FMIPA Universitas Riau Pekanbaru

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Kloning dan karakterisasi gen penyandi inhibitor proteinase dari kulit buah kakao Cloning and characterization of gene encoding proteinase inhibitor of cacao pod wall ISDA, Mayta Novaliza; KASIM, Musliar; MANSYURDIN, .; CHAIDAMSARI, Tetty; SANTOSO, Djoko
E-Journal Menara Perkebunan Vol 76, No 2: Desember 2008
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v76i2.84

Abstract

Summary Attempts to increase cocoa production in Indonesia have been hinderred by attack of CPB (Conopomorpha cramerella). There has been no effective measures to control this pest leading to development of cacao planting materials which resistant to the pod borer. One of genes functioning in plant defense system against insect pests such as catepilar is Proteinase Inhibitor (PIN). This research aimed to isolate and characterize TcPIN gene of cacao pod wall. A clone of TcPIN was isolated with RT-PCR technique using total RNA of cacao pod wall and DNA primer designed based on the sequence Trypsin Inhibitor of cocoa bean accessible online. BlastX analysis of the sequence of the cDNA clone demonstrated that the ± 600 bp gene cloned with pGEM-T was PIN gene as indicated by highly homologous to Trypsin Inhibitor of Theobroma microcarpum resulted in 248 Score bits and E value 1 e-64. Two sequence alligment with the putative 21 kDa PIN  of cacao seed indicated a moderately high homology. Contrasting these two sequences however found some non identical amino acids implying some variations. Ringkasan Usaha peningkatan produksi kakao di Indonesia terkendala antara lain oleh adanya serangan hama PBK (Conopomorpha cramerella). Untuk menanggulangi serangan PBK tersebut perlu adanya satu cara pengendalian yang efektif dan efisien, sehingga dapat mendorong usaha pengembangan bahan tanam yang tahan PBK. Salah satu gen  membawa sifat ketahanan tanaman terhadap hama ulat adalah Proteinase Inhibitor (PIN). Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi gen TcPIN dari kulit buah kakao. Klon cDNA TcPIN diisolasi dari kulit buah kakao dengan teknik RT-PCR meng-gunakan RNA total kulit buah kakao dan primer DNA yang dirancang atas dasar sekuen Inhibitor Tripsin biji kakao yang diakses lewat internet.  Hasil analisis BlastX dari sekuen klon cDNA menunjukkan  bahwa gen berukuran  ± 600 pb yang telah diklon dengan pGEM-T tersebut adalah PIN karena memiliki homologi yang tinggi terhadap 21 kDa trypsin inhibitor dari Theobroma microcarpum yang meng-hasilkan Skor 248 bits dengan Nilai E 1e-64. Penjajaran dua sekuen dengan PIN putatif 21 kDa yang berasal dari biji kakao menunjuk-kan tingkat homologi yang tinggi, dengan perbedaan nyata sehingga dapat terlihat bahwa keduanya tidak identik.
INDUKSI TUNAS IN VITRO JERUK SIAM (Citrus nobilis Lour.) ASAL KAMPAR DARI EKSPLAN TUNAS APEKS DAN NODUS IN VITRO Hidayati, Nurul; Lestari, Wahyu; Isda, Mayta Novaliza
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Citrus nobilis Lour. is a citrus plant that is highly demanded by many people in Riau. Propagation of C. nobilis Lour. has some problems due to various diseases that decrease its production. The conventional propagation of C. nobilis. requires a lot of parent plants and takes a long time, therefore the in vitro propagation is necessary. The purpose of this study was to determine the concentration of BAP and explant source as well as the interaction between both treatments in inducing C. nobilis bud. This research used a factorial randomized block design (RBD) with two factors i.e the concentration of BAP (0, 1, 2 and 3 mg / l) and the source of explants (shoot apex and 6 week in vitro nodes). All combined treatments showed no significant difference to the emerging bud, the number of shoots and length of shoot. The results showed that the addition of 2 mg/l BAP at the apex shoot and node explants were able to induce bud. Better explant sources in inducing bud was the node explants. The node explants at a concentration of 1 mg/l BAP gave better result in inducing bud.
INDUKSI TUNAS DARI EKSPLAN BIJI MANGGIS (Garcinia mangostana L.) ASAL BENGKALIS SECARA IN VITRO DENGAN PERLAKUAN BAP (Benzylaminopurine) PADA MEDIUM MS Rahmawati, Ria Yuni; Isda, Mayta Novaliza; Fatonah, Siti
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Mangosteen (Garcinia mangostana L.) from Bengkalis island has many superiorities such as growing in the marshes and tolerant to acid soil (peat soil). In vitro culture is an alternative way to get large number and uniform mangosteen seedling. The purpose of this research was to determine the best concentration of BAP (Benzylaminopurine) in inducing mangosteen’s shoots from Bengkalis. This research used Randomized Complete block Design (RAK) with 5 concentrations of BAP (0; 1; 3; 5; 7 mg/l) with 5 replications. The result of this research showed the fastest shoot forming time and highest number of shoots present in a concentration of 5 mg / l BAP at 13 DAP and 2 shoots per explant.
INDUKSI AKAR JERUK SIAM ASAL KAMPAR (Citrus nobilis Lour.) DARI TUNAS IN VITRO DENGAN BERBAGAI KOMBINASI SUKROSA DAN NAA PADA MEDIA ½ MURASHIGE AND SKOOG Wijayanti, Indah; Isda, Mayta Novaliza; Lestari, Wahyu
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

> Citrus (Citrus nobilis Lour.) from Kampar is one of primary commodity from Riau Province. In order to conserve citrus from Kampar as a main fruit from Riau, it is necessary to carry out seedling multiplication using in vitro method. The purpose of the study was to determine the best concentration of sucrose and NAA as well as to determine their interaction in inducing citrus root on ½ MS media. This research used Randomized Block Design (RBD) factorial with two factors, i.e. the concentration of sucrose (1, 2, 3 and 4%) and NAA (0, 0,5, 1 and 1 ,5 mg/l). The concentration of 1 and 2% sucrose produced the highest number of root (1,17 units), 2% sucrose could give the fastest root induction (17,6 days) and also produced the longest root (2,57 cm). The combination of 1% sucrose + 1 mg/l NAA could give the fastest root induction (11,5 days), while the combination of 3% sucrose + 0,5 mg/l NAA produced the longest root (2,31 cm) and 3% sucrose + 1,5 mg/l NAA produced the highest number of root (1,64 units).
POTENSI ALELOPATI EKSTRAK DAUN Gleichenia linearis (Burm.) Underw. TERHADAP PERKECAMBAHAN DAN PERTUMBUHAN ANAKAN GULMA Mikania micrantha (L.) Kunth Susanti, Awit Tatas Asih; Isda, Mayta Novaliza; Fatonah, Siti
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Gleichenia linearis is a wide-leaf weed that contains chemical compounds such as flavonoid, allelochemical, triterpenoid, saponin, tannin, alkaloid and steroid that can inhibit the growth of other plants so it can be used as bioherbicide. The aims of this study were to determine the potential of residues and the concentration of G. linearis leaf extract on germination and growth of seedling weed Mikania micrantha. This studywas conducted using completely randomized design with 5 replicates with three concentrations i.e. 10%, 20% and 30%. Data was observed and analyzed using Analysis of Variance (ANOVA) and tested using Duncan's Multiple Range Test (DMRT) if it showed significant effect. This study showed the decrease of growth and germination and the increase of the percentage of the death of M. micrantha weed after treatment of G. linearis leaf extract. The highest decrease was found in 30% extract concentration with the percentage of germination, fresh weight, the percentage of mortality were 19%, 0.436 g and 10%, respectively.
MORFOLOGI PERMUKAAN DAUN TANAMAN TERUNG (SOLANUM MELONGENA L.) SEBAGAI RESPONS TERHADAP CEKAMAN KEKERINGAN Ilahi, Rizky Nanda Kurnia; Isda, Mayta Novaliza; Rosmaina, Rosmaina
Al-Kauniyah: Jurnal Biologi Vol 11, No 1 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v11i1.5662

Abstract

Abstrak Terung (Solanum melongena L.) tergolong tanaman yang sensitif terhadap kekeringan selama tahap pertumbuhan dan perkembangannya. Karakteristik stomata dan trikoma merupakan kriteria yang dapat digunakan untuk mengidentifikasi tanaman yang toleran terhadap kekeringan. Penelitian bertujuan menguji respons karakteristik anatomi daun berupa trikoma dan stomata tanaman terung terhadap cekaman kekeringan melalui empat taraf interval penyiraman. Penelitian menggunakan Rancangan Acak Lengkap melalui empat taraf interval penyiraman, yakni 3, 6, 9, dan 12 hari.  Pengaruh cekaman kekeringan terhadap morfologi permukaan daun pada terung menyebabkan terjadinya peningkatan kerapatan trikoma tiga kali lebih banyak dibandingkan dengan kontrol, penurunan ukuran lebar trikoma mencapai 59,02%, penurunan ukuran lebar stomata mencapai 78,34%, dan penurunan ukuran lebar porus stomata mencapai 80,80%. Hasil penelitian menunjukkan bahwa peningkatan jumlah trikoma dengan ukuran trikoma yang semakin kecil diduga sebagai bentuk proteksi tanaman terhadap kerusakan jaringan dan mekanisme adaptasi tanaman untuk dapat memenuhi jumlah asimilasi CO2 perluas daun yang dibutuhkan untuk fotosintesis pada kondisi kekeringan. Sensitifitas tanaman terhadap kondisi kekeringan berupa mekanisme adaptasi dengan cara memperkecil ukuran stomata dan bukaan lebar porus, sehingga laju fotosintesis tetap terjaga pada kondisi kekeringandan mekanisme tanaman menjaga efisiensi penggunaan air dengan cara mengurangi ukuran stomata dan memperkecil bukaan porus stomata.    Abstract Eggplant (Solanum melongena L.) belongs to a group of plants that are sensitive to drought (water stress) during their growth and development stages. Characteristics of stomata and trichomes are criteria that can be used to identify drought-tolerant plants. This study aims to determine the response of leaf anatomical characteristics of the eggplant as well as trichome and stomata to drought stress through four levels of watering interval. The research using completely randomized design with watering intervals of 3, 6, 9, and 12 days. The effect of drought stress on leaf surface morphology of the eggplant resulted in three times greater trichomes density than control, decreased trichomes width by 59.02%, stomata width by 73.84%, and size of stomata porch width by 80.80%.. The result was showing that increasing number of trichome with smaller trichome size was thought to be a form of crop protection against tissue damage and plant adaptation mechanism in order to meet the amount of CO2 leaf expansion assimilation required for photosynthesis in drought stress condition. The sensitivity of plants to drought stress conditions is the mechanism of adaptation by reducing the size of stomata and wide porous opening, so that the rate of photosynthesis has been maintaining in the dry conditions and the mechanism of the plant maintain the efficiency of water use by reducing the size of stomata and minimizing stomata porous opening.   Permalink/DOI: http://dx.doi.org/10.15408/kauniyah.v11i1. 5667
Penaksiran Biomassa dan Karbon Tersimpan pada Ekosistem Hutan Mangrove di Kawasan Bandar Bakau Dumai MANDARI, DESTI ZARLI; GUNAWAN, HARIS; ISDA, MAYTA NOVALIZA
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Mangrove forest ecosystem has a function as an absorber of Carbon dioxide (CO2) from the air and storecarbon in the form of biomass. The research was conducted in December 2014 until January 2015, andaimed to determine the total amount of above-ground biomass and the carbon stock as well as therelation of the three conditions of the development of mangrove forests in the area of Bandar Bakau,Dumai, Riau Province. The plot was made by using transect line plot method, 50 x 50 m in size with atotal of 3 plots along the coastline. Each plot contained 5 (five) sub-plots (10 x 10 m) that randomlydetermined. The results showed that along the transect line was found 10 species of mangrove, i.e.Xylocarpus granatum, Rhizophora apiculata, Rhizophora mucronata, Bruguiera gymnorrhiza, Avicenniaalba, Avicennia marina, Sonneratia alba, Lumnitzera racemosa, Lumnitzera littorea, and Scyphiphorahydrophyllacea. The vegetation was dominated by Xylocarpus granatum with a density of 2128 trees/haand Rhizophora apiculata with a density of 1472 trees/ha. The highest total biomass and carbon stockgenerated by X. granatum with total biomass 78.6 ton/ha equivalent to 39.3 ton of C/ha. The total value ofbiomass produced from the whole plot is 115.85 ton/ha with a total carbon content stored at 57.91 tonC/ha.Key words: Above-ground Biomass, Carbon stored, Mangrove density, Xylocarpus granatum
Tingkat Kerusakan Ekosistem Mangrove di Desa Teluk Belitung Kecamatan Merbau Kabupaten Kepulauan Meranti UMAYAH, SARI; GUNAWAN, HARIS; ISDA, MAYTA NOVALIZA
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

The destruction of mangrove forest ecosystems in Teluk Belitung Village, Kepulauan Meranti, wascaused by various human activities such as industrial activities, illegal logging, and coastal erosion.Rehabilitation efforts was needed to preserve mangrove forests. The community involvement had animportant role in determining the success of mangrove rehabilitation. This study aimed to determine theextent of mangrove forest ecosystem destruction based on the density and mangrove forest cover.Furthermore, this research described local community participation level and their knowledge inrehabilitation efforts of mangrove forest ecosystem. This study was conducted in Teluk Belitung,Subdistrict of Merbau, Kepulauan Meranti from November 2014 to March 2015. This study usedobservation, questionnaire, and interview methods to investigate the destruction of mangrove forestsecosystem and local community involvement in mangrove forests rehabilitation. The results showed thatthe region has suffered damage to the mangrove with a total density value of 626.67 trees/ha andmangrove forest cover in all types of <50%. The level of public participation was affected by socialstatus, planning, attitudes, and community rules in Teluk Belitung.Keywords: mangrove, community, participation, rehabilitation
Induksi Tunas dari Eksplan Kotiledon dan Epikotil In Vitro Jeruk Siam (Citrus nobilis Lour.) Asal Kampar pada Media MS CAHYATI, SRI; ISDA, MAYTA NOVALIZA; LESTARI, WAHYU
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Citrus nobilis Lour. (Siam Orange) from Kampar is one of the main fruits in Riau Province that is highlyconsumed by many people, but nowadays its productivity is lower due to pests and diseases. In order tomaintain the availibility of citrus from Kampar and to conserve this plant, an effort that can be done is byusing in vitro culture with the use of a combination of growth regulator; cytokine and auxin usingcotyledons and epicotyl explant. This study aimed to determine the best explant source and to know theeffect of the concentration combination of BAP, kinetin, and NAA that optimal in inducing plant shoot ofSiam orange. This study was conducted at Laboratory of Integrated Biology, Department of Biology,Faculty of Mathematics and Natural Sciences using a completely randomized design (CRD) with variousconcentrations of treatments, and observed for 42 days. The results showed that the percentage of lifeexplants, swelling cotyledon, and epicotyl explants was 100%, the percentage of best shoot formation was60% on the epicotyls explant with 1.0 mg/l BAP + 0.5 mg/l kinetin + 0.5 mg/l NAA. The percentage ofcallus formation only found in epicotyls explants with the highest percentage (100%) at 2.0 mg/l BAP +0.5 mg/l kinetin +1.0 mg/l NAA.Keywords: Citrus nobilis Lour., cotyledons, epikotil, in vitro, shoot induction.
Induksi Tunas in vitro Jeruk Siam (Citrus nobilis Lour.) Asal Kampar pada Berbagai Konsentrasi Sukrosa FATONAH, SITI; ISDA, MAYTA NOVALIZA; LESTARI, WAHYU
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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This study aimed to determine the most effective concentration of sucrose for a shoot induction fromcotyledon explants of Citrus nobilis from Kampar. Cotyledon explants were cultured on Murashige andSkoog (MS) medium containing sucrose at various concentrations without supplemented with growthregulators. The treatments consisted of five concentrations of sucrose, i.e. 0, 20, 30, 40, 50, 60 g /l. Theresult showed that all explants on different concentrations of sucrose treatment had the percentage ofliving and the percentage of shoot induction reached 100%. The best response for shoot induction aftereight weeks of culture was observed on MS medium supplemented with 50 g/l sucrose with the number ofshoot was 2, shoot length was 4,125 cm, and leaf number was 5,74 strands.Key words: Citrus nobilis lour., concentration of sucrose, in vitro, shoot induction