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ISOLATION OF CELLULOLYTIC BACTERIUM STAPHYLOCOCCUS SP. JC20 FROM THE INTESTINE OF OCTOPUS (OCTOPUS SP.) FOR FISH PROBIOTIC CANDIDATE Istiqomah, Indah; Isnansetyo, Alim; Atitus, Imelda Novita; Rohman, Ahmad Fauzi
Jurnal Perikanan Universitas Gadjah Mada Vol 21, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (488.353 KB) | DOI: 10.22146/jfs.39525

Abstract

Aim of this study was to isolate, characterize, and identify cellulolytic bacteria from the digestive tract of marine vertebrates and invertebrates as a candidate of fish probiotics. The bacteria were isolated from the digestive tract and grown on a cellulose agar plate. The bacteria were screened based on the cellulolytic activity, acid resistance, antagonist activity against fish pathogens, antibiotics sensitivity, ability to live in fish digestive tract and non-pathogenic test. Selected bacterium was identified molecularly, based on the 16S rDNA gene sequences, and phenotipically. A total of 14 bacteria demonstrated celulolitic index of 1.1-1.8. The bacteria with cellulolytic index of > 1.6 were screened by the selection criteria, resulted a selected strain, JC20 isolate which was isolated from the digestive tract of octopus (Octopus sp.). The selected bacterium was sensitive to antibiotics, resists to acidic environment, able to live in the fish digestive tract, and non-pathogen. Thus, the bacterium was potential for further characterization as fish probiotics candidate. Molecular and phenotypic identification revealed that JC20 isolate was Staphylococcus sp.
Characterizing the Three Different Alginate Type of Sargassum siliquosum Yudiati, Ervia; Isnansetyo, Alim
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 22, No 1 (2017): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (349.989 KB) | DOI: 10.14710/ik.ijms.22.1.7-14

Abstract

This research was aimed to identify the brown seaweed, to characterize the acid, sodium and calcium alginate, and to examine the alginate yield. The identification was done phaenotypically. The extraction method was pretreated by ethanol depigmentation, followed by the extraction of Na2CO3/EDTA and CaCl2  and presipitated with absolute ethanol. The characterization of alginate was done by FT-IR spectroscopy and Thin Layer Chromatography by comparing the samples with standard alginate (Sigma, USA). The key of identification  showed that the species was Sargassum siliquosum. There are similarities in signal vibration and TLC spots among the samples and the standard. The TLC test was also showed that those alginates contain mannuronic and guluronic acid. The highest yield was produced by Sodium alginate (40,34% + 0,21), followed by Acid alginate (11,51% + 0,15) and Calcium alginate (4,8% + 0,09). Keywords: alginate, characterization, Sargassum siliquosum, yield
METODE ANTAGONISTIK LANGSUNG UNTUK SKRINING BAKTERI LAUT PENGHASIL SENYAWA ANTI-METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) Isnansetyo, Alim; Kamei, Yuto
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (217.974 KB) | DOI: 10.24002/biota.v10i3.2873

Abstract

Metode yang berbasis antagonistik langsung antara bakteri laut dan Staphylococcus aureus resistan metisilin (Methicillin-resistant Staphylococcus aureus, MRSA) berhasil digunakan untuk skrining bakteri laut penghasil senyawa anti-MRSA. Metode ini menggunakan medium agar lapis ganda untuk mendukung pertumbuhan bakteri laut dan MRSA. Beberapa bakteri laut penghasil senyawa anti-MRSA berhasil diisolasi dengan metode ini. Suatu bakteri laut, strain O-BC30 merupakan bakteri yang paling kuat menghambat pertumbuhan MRSA. Isolat-isolat bakteri yang didapat menunjukkan aktivitas yang stabil terhadap MRSA dan aktivitas ini juga konsisten dengan aktivitas ekstrak yang diperoleh dari sel bakteri yang dikultur pada medium agar. Metode ini sangat berguna dan efisien untuk skrining bukan hanya bakteri penghasil senyawa anti-MRSA tetapi juga bakteri penghasil antibiotik.
Independent Effects of Temperature, Salinity, Ammonium Concentration and pH on Nitrification Rate of the Ariake Seawater Above Mud Sediment ISNANSETYO, ALIM; GETSU, SAI; SEGUCHI, MASAHIRO; KORIYAMA, MASUMI
HAYATI Journal of Biosciences Vol. 21 No. 1 (2014): March 2014
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (915.094 KB) | DOI: 10.4308/hjb.21.1.21

Abstract

The Ariake Sea located in the west parts of Kyushu Island is a semi-closed and macro-tidal shallow sea, and has the largest tidal flat in Japan. A large mud tidal flat with a productive ecosystem found along the western shoreline of the sea makes this area ideal as a major production site of nori (Porphyra yezoensis) in Japan. We determined the independent effect of temperature, salinity, ammonium concentration and pH on nitrification rates (NR) in the Ariake seawater above the mud sediment. The NR was determined by measuring accumulation of NO2-N production after adding sodium chlorate, an inhibitor of NO2-N to NO3-N oxidation. NRs were relatively high at 20-35 oC (optimum at 29.5 oC), but the rates were very low at 5, 10, and 40 oC. NRs increased sharply when increasing the salinity from 13 to 20 ppt, but it decreased drastically at salinity levels more than 35 ppt (optimum at 19 ppt). The relationship between ammonium concentration and NR showed a typical kinetic curve of enzymatic reaction with the maximum NR (Vmax) of 0.029 mM N.h-1 at 200 mM NH4-N (the half saturation constant (Ks) = 35 mM NH4-N). High NRs were determined at pH 7.5-8.0 (optimum pH 7.8). This is the first report on the independent effects of temperature, pH, salinity and NH4-N concentration on the NR of seawater, specifically the Ariake seawater.
In vitro activity test is a critical evaluation to screen the potential biocontrol agent. We developed a selective medium for quantitative in vitro activity evaluation of bacterial biocontrol agents against pathogenic Vibrio in aquaculture. Sensitivity test of bacterial biocontrol and Vibrio spp. to nine antibiotics showed that oxytetracycline inhibited the growth of Vibrio spp., but did not inhibit the growth of the bacterial biocontrol. This selective inhibition activity of oxytetracycline sug ISNANSETYO, ALIM; MUHTADI, .; ISTIQOMAH, INDAH; NITIMULYO, KAMISO HANDOYO; TRIYANTO, .
HAYATI Journal of Biosciences Vol. 18 No. 3 (2011): September 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.3.129

Abstract

In vitro activity test is a critical evaluation to screen the potential biocontrol agent. We developed a selective medium for quantitative in vitro activity evaluation of bacterial biocontrol agents against pathogenic Vibrio in aquaculture. Sensitivity test of bacterial biocontrol and Vibrio spp. to nine antibiotics showed that oxytetracycline inhibited the growth of Vibrio spp., but did not inhibit the growth of the bacterial biocontrol. This selective inhibition activity of oxytetracycline suggested this antibiotic might be supplemented to establish a selective medium. The MIC of oxytetracycline against V. harveyi, V. parahaemolyticus, and V. algosus were 120, 250, and 120 mg/ml, respectively. These concentration did not inhibit the growth of bacterial biocontrols. Therefore, oxytetracycline was supplemented at 250 µg/ml in Zobell agar medium. This medium was used as a selective medium to enumerate the density of bacterial biocontrol. In vitro activity test of bacterial biocontrol (RLP1) against V. parahaemolyticus showed that strain RLP1 at density of 104 and 106 cells/ml was able to kill V. parahaemolyticus during 6 h incubation. At lower density, 102 cells/ml, this bacterial biocontrol agent was able to kill the pathogenic Vibrio during 12 h incubation. This study discovered a selective medium for the bacterial biocontrol and Vibrio spp. and provided the results of its application in the evaluation of in vitro activity of a bacterial biocontrol agent against V. parahaemolyticus. The results also revealed that strain RLP1 is a potential bacterial biocontrol against vibriosis in marine aquaculture.
IMMUNOSTIMULATORY EFFECT OF FATTY ACID FROM STAR FISH (ACANTHASTER PLANCI) ON LYMPHOCYTE PROLIFERATION IN-VITRO Achmad, M. Janib; Isnansetyo, Alim; Kasanah, Noer; Ustadi, Ustadi; Kamiso, Kamiso
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 9, No 3 (2014): December 2014
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v9i3.109

Abstract

The aims of this study were to investigate lymphocyte proliferation activity and to identify chemical constituents of active fractions of star fish Acanthaster planci. A. planci was collected from Ternate Island, North Moluccas, extracted with distilled methanol and water, partitioned with gradient chloroform-hexane-methanol-water and fractionated in column chromatography using silica gel and hexane-ethyl acetate-methanol. The active compound had been purified by Thin Layer Chromatography (TLC) and identified by Gas Chromatography-Mass Spectrometry (GC-MS). The lymphocyte proliferation activity was measured based on % Stimulation Index (SI) from sample absorbency and control absorbency. The result showed that the 3 fractions of hexane fraction exhibited lymphocyte proliferation activity. Fraction 1 was able to increase lymphocyte proliferation at 48 hours and 72 hours by 55% (80 µl/ml) and 88% (160 µl/ml) increase, and fraction 2 had 77% (160 µl/ml) and 86% (640 µl/ml) increase. Meanwhile, fraction 3 had 75% (640 µl/ml) and 89% (640 µl/ml) increase. Metabolite analysis of active fraction using GC-MS yielded a number of chemical constituents that was dominated by fatty acid. The study concluded that star fish A. planci from Ternate Island has a potential source of immunostimulator.
Antibacterial Compounds from Red Seaweeds (Rhodophyta) Kasanah, Noer; Triyanto, Triyanto; Seto, Drajad Sarwo; Amelia, Windi; Isnansetyo, Alim
Indonesian Journal of Chemistry Vol 15, No 2 (2015)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.461 KB) | DOI: 10.22146/ijc.21215

Abstract

Seaweeds produce great variety of metabolites benefit for human. Red seaweeds (Rhodophyta) are well known as producer of phycocolloids such agar, agarose, carragenan and great variety of secondary metabolites. This review discusses the red algal secondary metabolites with antibacterial activity. The chemical constituents of red algae are steroid, terpenoid, acetogenin and dominated by halogenated compounds mainly brominated compounds. Novel compounds with intriguing skeleton are also reported such as bromophycolides and neurymenolides. In summary, red seaweeds are potential sources for antibacterial agents and can serve as lead in synthesis of new natural medicines.
EFEKTIVITAS VAKSIN POLIVALEN UNTUK PENGENDALIAN VIBRIOSIS PADA KERAPU TIKUS (Cromileptes altivelis) Nitimulyo, Kamiso Handoyo; Isnansetyo, Alim; Triyanto, Triyanto; Murdjani, Muhammad; Sholichah, Lili
Jurnal Perikanan Universitas Gadjah Mada Vol 7, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9056

Abstract

The objective of this research was to know the effectiveness of polyvalen  Vibrio vaccine to control vibriosis in humpback grouper (Cromileptes altivelis). The effectiveness of vaccine was evaluated by the survival rate (SR), relative percent survival (RPS), mean time to death (MTD) as well as growth rate of vaccinated fish. This research consisted of 4 treatments (control, injection, immersion, and oral vaccinations) in quadruplicates. Injection  vaccination was conducted by intraperitoneal injection of polyvalen vaccine at 107 cells/fish. Immersion vaccination was done by immersing the fishes at 107 cells/ml for 30 minutes. Oral administration of vaccine was also carried out  at 107 cells/fish. One week after the first vaccination, second vaccination (booster) was carried out at the same dosage and by the same administration. One week after the second vaccination, fishes were challenged with 3.16x104 cells/fish of Vibrio ordalii 3J by intraperitoneal injection, and reared for 20 days post infection. Results indicated that polyvalen Vibrio vaccine increased SR (P<0.01) up to 100%. Vaccination was also able to delay MTD of fishes. However, the vaccination was not influence the growth rate of fish.
EFEKTIVITAS EKSTRAK BAWANG PUTIH (Allium sativum) UNTUK MENCEGAH DAN MENGOBATI MOTILE AEROMONAS SEPTICEMIA (MAS) PADA IKAN MAS (Cyprinus carpio) Lukistyowati, Iesje; Windarti, Windarti; Morina, Morina; Isnansetyo, Alim; Kurniasih, Kurniasih
Jurnal Perikanan Universitas Gadjah Mada Vol 10, No 1 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.8921

Abstract

The aim of study was to know the effectiveness of garlic (Allium sativum) extract in preventing and curing Motile Aeromonas Septicemia (MAS) in common carp (Cyprinus carpio). Garlic extract was obtained by juicing fresh garlic and filtering by using filter paper. Common carp (8-12 cm in total lenght) were used. Infection was done by injecting intraperitonially 0.1 ml 0f 24 h Aeromonas hydrophila culture at bacterial density of 108 cells/ml. Garlic extract was given by immersing the fish at various concentrations. Results indicated that the minimum inhibitory concentration (MIC) of the garlic extract againtst A. hydrophila ranged from 61.300-125.000 ppm, while the LD50 of this extract was 3.438 ppm. The use of garlic extract to prevent MAS was effective as survival rate of the treated fish with the extract at 1000, 2000, and 3000 ppm ranged from 83 to 100% while the survival rate of untreated fish was 60.71%. However, the use of garlic extract to cure MAS was not effective as the survival rate of the treated fish ranged 50-80% which were the same or even lower than that of the control (76.67%).
PENAMBAHAN VITAMIN C PADA IKAN LELE DUMBO (Clarias gariepinus) UNTUK MENINGKATKAN TANGGAP KEBAL TERHADAP VAKSIN Aeromonas hydrophilia Isnansetyo, Alim
Jurnal Perikanan Universitas Gadjah Mada Vol 1, No 1 (1996)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.8846

Abstract

Motile Aeromonas Septicimia is a dangerous disease on fresh water fish especially walking catfish and carp. Vaccination against the disease has been developed in Indonesia. However, there were high result variations of the vaccination. Immune response is influenced by level of vitamin C dietery. Objective of this experiment was to know effect of vitamin c levels on walking catfish’s specific immune respose. The levels of vitamin (0, 50, 500, and 1000 mg ascorbic acid (AA)/kg feed) were given to the fish. Immersion vaccination for 30 minutes (108 sel/ml) was carried out 15 days after feeding trial. Challenge test was conducted by injection of 0,2 ml bacteria suspension (LD80 concentration) was carried out 15 days after vaccination. Growth rate of the fish was observed monthly. Result of this experiment indicated that mortality in the challenge test due to A. hydrophila infection considerably decreased for treatment combination among vaccination; and 500 and 1000 mg AA/kg feed. These AA levels without vaccination could not reduce mortality.