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THE ETHYL ACETATE FRACTION OF TORBANGUN (COLEUS AMBOINICUS L.) LEAVES INCREASING MILK PRODUCTION WITH UP-REGULATED GENES EXPRESSION OF PROLACTIN RECEPTOR Iwansyah, Ade Chandra; Damanik, Rizal Martua; Kustiyah, Lilik; Hanafi, Muhammad
Journal of Tropical Life Science Vol 9, No 2 (2019)
Publisher : Journal of Tropical Life Science

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Abstract

This study aim was to determine lactagogue effect of torbangun leaves to plasma levels of lactogenic hormone and gene expression of their receptors in mammary glands of lactation rats. Lactagogue activity was evaluated by volume of milk was produced by the rats treated with commercial milk booster contained ?katuk' leaves extract (AF), ethyl acetate fraction of torbangun leaves (EA), water extraction of torbangun leaves (AQ) and kaempferol (KP). Lactating rats (n=5) of Sprague dawley with six pups were fed with AF, EA, AQ, and KP in the amount of 50 mg/kg, 30 mg/kg, 80 mg/kg and 60 mg/kg body weight, respectively. The feed was given orally every two days and starting from day 2 after giving birth until day 28. The volume of milk was estimated by the increment pup weight after breastfed. The levels of serum lactogenic hormones were determined by ELISA methods. Moreover, in order to measure the gene expression of the lactogenic hormone's receptors in the mammary gland a real time - reverse transcription polymerase chain reaction (RT-PCR) method was performed. The results showed that ethyl acetate fraction of torbangun leaves (EA) (a) was not significantly stimulating the synthesis of serum prolactin and estradiol at day 14 and day 28 lactation period, (b) down-regulated the gene expression of estradiol receptor (ER?) at day 28, and (c) up-regulated the gene expression of prolactin receptor (PRLR) in mammary gland at day 14 and day 28. This study indicated that ethyl acetate fraction of torbangun leaves was induced milk production, within up-regulated the gene expression of prolactin receptor (PRLR) in the mammary gland of lactation rats.
POTENSI FRAKSI ETIL ASETAT DAUN TORBANGUN (COLEUS AMBOINICUS L.) DALAM MENINGKATKAN PRODUKSI SUSU, BOBOT BADAN INDUK, DAN ANAK TIKUS Iwansyah, Ade Chandra; Damanik, Muhammad Rizal Martua; Kustiyah, Lilik; Hanafi, Muhammad
Jurnal Gizi dan Pangan Vol. 12 No. 1 (2017)
Publisher : Food and Nutrition Society of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (464.182 KB) | DOI: 10.25182/jgp.2017.12.1.61-68

Abstract

The aims of the research were to investigate the potential medicine from ethyl acetate fraction of Coleus amboinicus L. for improving milk yield, body weight of rats and its effects on growth of the rat pups. Galactagogue activity was evaluated in terms of quantity of milk produced from the rats treated with ethyl acetate fraction of torbangun leaves (FEA), and commercial milk booster contained ?katuk? leaves extract (AF). Lactating rats (n=5) of Sprague dawley with six pups were fed with FEA and AF in the amount of 30 mg/kg and 50 mg/kg body weight, respectively. The feed were given orally every two days and starting from day 2 after giving birth until day 14. The performance of milk production was measured along the experimental period by weight-suckle-weight method. The rates of pups? growth were measured as the weight gain along experimental period. The rats fed with ethyl acetate fraction of torbangun leaves produced higher milk than AF and the control groups, while FEA did not affected the body weight of rats in comparison to the control groups. Ethyl acetate fraction of torbangun leaves was identified to increase milk production by 17%, while AF by 22%. The mean of yields produced by the rats during lactation period for FEA, AF and control were 7.12±1.16, 7.43±1.04 and 6.07±0.85 g/pups/day, respectively. The FEA administration showed gradually increased of rat pups growth and body weight gain significantly in pups 2-14 days old compared to the control group. The present study reveals the ethyl acetate fraction has the potency as a remedy for improving milk yield without influencing the rats.
Determination of Level of Food Additives in Labisia pumila (LP) Beverages Consumed in Kuantan, Malaysia Iwansyah, Ade Chandra; Yusoff, Masithah Mohammad; Kormin, Faridah
Agritech Vol 32, No 4 (2012)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (232.211 KB) | DOI: 10.22146/agritech.9575

Abstract

The content levels of several food additives (gallic acid, benzoic acid and caffeine) in commercial Labisia pumila (LP) beverage samples in Kuantan, Malaysia were determined by high performances liquid chromatography (HPLC). These analytical measurements were undertaken primarily to assess the compliance of content levels of the investigated food additives and their daily intake doses with permissible levels. The results obtained from this study indicated that the average levels of GA, caffeine and benzoic acid in the analyzed beverages were 37.62-229.35 ppm, 43.46 -168.00 ppm and 98.10-241.13 ppm, respectively. In addition, the concentrations of these food additives have been converted into daily intake doses based on beverage consumption. It was estimated that the mean daily intake of GA, caffeine and benzoic acid  by the adult population of Kuantan through the consumption of the analyzed beverages  were 0.39 mg/kg body weight/day for GA, 0.59 mg/kg body weight/day for caffeine (19.6 % ADI) and 0.43 mg/kg body weight/day for benzoic acid (8.6%ADI). None of the analyzed beverage sample was found to violate the current legal limits as stipulated in Malaysian food regulation.
IDENTIFIKASI DAN KUANTIFIKASI ASAM GALAT SEBAGAI SUMBER ANTIOKSIDAN PADA EKSTRAK DAUN KACIP FATIMAH (Labisia pumila var. alata) LARUT AIR Iwansyah, Ade Chandra; Yusoff, MM
Jurnal Aplikasi Teknologi Pangan Vol 2, No 3 (2013): Agustus 2013
Publisher : Indonesian Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (459.038 KB)

Abstract

Labisia pumila (Myrsinaceae) atau "Kacip Fatimah", secara tradisional digunakan dalam pengobatan tradisional Melayu sebagai tonik setelah melahirkan. Saat ini di Malaysia, Labisia pumila begitu populer sebagai makanan atau minuman fungsional. Perusahaan mengimpor bahan baku tanaman ini untuk memenuhi permintaan konsumen. Namun, informasi mengenai senyawa aktif dari tanaman ini sangat jarang dan langka. Tujuan penelitian ini adalah untuk mengidentifikasi, menghitung serta mengetahui pengaruh asal tumbuh terhadap kandungan asam galat Labisia pumila var alata (LP) asal Indonesia. Identifikasi dan kuantifikasi asam galat diukur dengan menggunakan metode kromatografi cair berkinerja tinggi (KCKT). Hasil menunjukan ekstrak LP larut air asal Indonesia memiliki karakteristik fisik: rendemen (10-11% b/b), total padatan (1.33˚brix) dan kelarutan dalam air 88% (b/b) (air dingin) dan 93% (b/b) (air panas). Ekstrak LP larut air yang berasal dari Gunung Tilu, Bogor memiliki kandungan asam galat (GA) tertinggi (1.86% b/b) dibandingan ekstrak LP larut air dari daerah lain. Kandungan asam galat (GA) pada tanaman LP dipengaruhi oleh faktor lokasi dan tempat asal tumbuh (p ≤ 0,01), tetapi tidak dengan karakteristik fisik (rendemen, total padatan dan kelarutan) (p ≥ 0.05). LPT memiliki sumber antioksidan alami, yang bermanfaat bagi kesehatan manusia dan dapat digunakan sebagai makanan fungsional untuk memenuhi kebutuhan diet.
Minerals and heavy metals in Labisia pumila var. alata folia of Selected Geographic Origins Iwansyah, Ade Chandra; Abidin, Noorazizah Zainal; Yusoff, Mashitah Mohammad
Jurnal Kartika Kimia Vol 2 No 1 (2019): Jurnal Kartika Kimia
Publisher : Department of Chemistry, Faculty of Sciences and Informatics, Jenderal Achmad Yani University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jkk.v2i1.25

Abstract

Labisia pumila var. alata leaves (LP) with a long history of use folk remedy and endemic to the Malay Archipelago, is now supplied worldwide as ingredient of functional foods and beverages. Minerals and heavy metal concentrations in Labisia pumila var. alata folium (LP) of selected geographic origin based on microwave-assisted sample digestion and inductively coupled plasma mass spectrometry (ICP-MS) were determined.  Fifteen elements comprising minerals (Ba, Ca, Cr, Co, Cu, Fe, K, Mg, Na, Ni, Rb and Zn) and heavy metals (As, Cd and Pb) were analyzed with an inductively coupled plasma – mass spectrometer (ICP-MS). The highest nutrient concentration was measured in LP from Tilu Mountain (Cu, Na, K, Mg and Zn). The highest values of Ba, Ca, Co, Cr and Fe were detected in LP from Raub. Ni and Rb were highest in LP from Cibeundey Village. As was highest in LP from Raub (0.04 ± 0.00 mg/kg). The highest Pb contents were in LP from Tilu Mountain (2.90 ± 0.10 mg/kg) and LP from Halimunan-Salak Mountain (3.12 ± 0.03 mg/kg), all of which were well within the permissible limits as specified by the U.S. FDA for edible plant parts.  
PHYSICOCHEMICAL, MINERALS AND ANTIOXIDANT PROPERTIES OF Labisia pumila var. alata OF SELECTED GEOGRAPHIC ORIGIN Iwansyah, Ade Chandra; Yusoff, Mashitah Mohd
AGRIVITA, Journal of Agricultural Science Vol 34, No 1 (2012)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v34i1.77

Abstract

In this study, relationship between physicochemical, mineral and antioxidant properties of water-soluble extracts of Labisia pumila var. alata is proposed. A wide range of analytical parameters were studied including physicochemical viz., proximate composition, yield, pH, total solid, total acidity, solubility; minerals content (Ca, Fe, and Zn) and heavy metals (Cd, Hg, As, and Pb); total phenolic content (TPC), tannins content, gallic acid (GA) content, and free scavenging activity of DPPH (EC50). Data were analyzed by ANOVA and principal component analysis (PCA). Based on the PCA analysis, TPC, GA content, moisture content, crude protein, and zinc content havesignificantly positively correlated (r close to 1) to antioxidant activity. Cluster analysis (CA) resulted in four groups of LP.These results provide useful data about the studied relationships and chemical patterns in LP leaves.
PENGARUH KONSENTRASI NATRIUM KLORIDA DAN LAMA FERMENTASI PADA MUTU FISIKOKIMIA, MIKROBIOLOGI, DAN SENSORI KIMCHI REBUNG Iwansyah, Ade Chandra; Patiya, Luthfiara Ghiyats; Hervelly, Hervelly
Industria: Jurnal Teknologi dan Manajemen Agroindustri Vol 8, No 3 (2019)
Publisher : Department of Agro-industrial Technology, University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.industria.2019.008.03.7

Abstract

AbstrakIndonesia adalah negara dengan keanekaragaman hayati kedua terbesar di dunia. Banyak di antaranya merupakan tumbuhan yang bersifat fungsional dan memiliki manfaat untuk kesehatan manusia, salah satunya yaitu rebung. Perendaman dan perebusan merupakan cara umum yanng sering digunakan untuk pengolahan rebung sehingga rebung memiliki daya simpan yang rendah. Pengolahan lanjutan diperlukan untuk mempertahankan daya simpan rebung sebagai pangan fungsional, terutama melalui cara fermentasi. Tujuan penelitian ini adalah mempelajari efek konsentrasi garam dan lama fermentasi terhadap mutu fisiko-kimia, total bakteri asam laktat, dan organoleptik kimchi rebung (Deandrocalamus asper). Desain eksperimental dengan rancangan acak kelompok (RAK) dengan pola faktorial. Faktor kesatu (?) yaitu konsentrasi garam (3%, 5% dan 7%) dan yang kedua (?) yaitu lama waktu fermentasi (2 hari, 4 hari dan 6 hari) dengan tiga kali pengulangan. Karakteristik fisikokimia, total bakteri asam laktat dan organoleptik dari kimchi rebung dianalisis. Berdasarkan hasil penelitian didapatkan bahwa terdapatnya pengaruh konsentrasi garam, lama fermentasi serta interaksi keduanya terhadap kadar pH, serat kasar, dan daya terima rasa kimchi rebung. Total bakteri asam laktat rebung dan persen kadar air dipengaruhi dengan baik oleh konsentrasi garam, sedangkan kadar abu, lemak, protein, warna, aroma dan tekstur kimchi rebung dipengaruhi oleh lama fermentasi.Kata kunci: bakteri asam laktat, fermentasi, karakteristik kimia, kimchi, rebung AbstractIndonesia has abundant natural resources. Various types of plants can grow well and have properties that are beneficial to human health. One of the plants that grow well in Indonesia is bamboo shoots. Bamboo shoots has been being processed by soaking and boiling only, but the shelf life of bamboo shoots is still very low. Further processing to extend the shelf life of bamboo shoots as food and functional food should be done through fermentation. This study aims to determine the effect of salt concentration and fermentation time in physicochemical, microbial characteristics, and organoleptic of bamboo shoot kimchi (Dendrocalamus asper). The experimental design was a factorial randomized block design (RBD). The first factor (?) was salt concentration (3%, 5% and 7%) and the second factor (?) was duration of fermentation (2 days, 4 days and 6 days) with three times replication. Physicochemical characteristics, total lactic acid bacteria, and organoleptic of bamboo shoot kimchi were analyzed. The results showed that the concentration of salt, the duration of fermentation, and its interaction influenced pH value, crude fiber, and the taste of bamboo shoots kimchi. The salt concentration affected the moisture content and total lactic acid bacteria of bamboo shoots, meanwhile ash content, fat, protein, color, aroma, and texture of bamboo shoot kimchi influenced by fermentation time.Keywords: bamboo shoots, fermentation, kimchi, lactic acid bacteria, physicochemical