Agoes Mardiono Jacoeb
Departemen Teknologi Hasil Perairan Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor. Jalan Lingkar Akademik, Kampus IPB Dramaga-Bogor 16680 Telepon +622518622915

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PURIFIKASI KOLAGENASE DARI USUS BANDENG (CHANNOS CHANNOS, FORSKAL) Yuniarti, Tatty; Nurhayati, Tati; Jacoeb, Agoes Mardiono
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2593

Abstract

Collagenase was purified from intestines of milkfish (Chanos chanos, Forskal) by extraction,ammonium precipitation, ion exchange chromatography on DEAE Sephadex A-50 and gelfiltration on a Sephadex G-100 column. The purification and yields were 114.731 fold and1.26% when compared to those in the starting-crude extract. The molecular mass of milkfishserine collagenase was estimated to be 14.63 kDa and 27.46 kDa.
EXTRACTION AND CHARACTERIZATION OF LIVER OIL FROM SILKY SHARK Rozi, Anhar; Suseno, Sugeng Heri; Jacoeb, Agoes Mardiono
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 19 No. 2 (2016): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (689.891 KB) | DOI: 10.17844/jphpi.v19i2.13453

Abstract

Silky shark liver was 10-15% of its body mass and 50% of oil is contained in the liver. The aimed ofthis study was to determine heavy metal content, fatty acid profile on raw material, extraction of its oil, andparameter of oxidation. The method of heavy metal test stated on the SNI and fatty acid analysis referredto AOAC. The extraction was oven methode at 50, 60, 70, and 80°C in 8 hours. Cadmium (Cd) as thehighest heavy metal content with 0.88±0.01 ppm, SFA was 18.46% composed by palmitic acid dominantly(12.59%). The MUFA was 24.54 % with the highest oleic acid (17.86%). PUFA was 19.11 % that consist ofDHA (14.35%) as the most abundantly present while EPA was 1.50%. Shark liver oil which was extractedat 50°C had the highest yield (24.47%). The oxidation of silky shark liver oil extraction indicated the besttreatment at 50°C with peroxide value (PV), p-Anisidine value (p-AV), percentage of free fatty acid (% FFA),acid value (AV), and total oxidation (TOTOX) were 7.26±1.27 mEq/kg, 16.79±0.28 mEq/kg, 5.47±0.12%,10.88±0.25 mg KOH/kg, and 31.31±2.26 mEq/kg respectively.
CHEMICAL COMPOSITION, BIOACTIVE COMPONENTS AND ANTIOXIDANT ACTIVITIES FROM ROOT, BARK AND LEAF LINDUR Dia, Siluh Putu Sri; Nurjanah, N.; Jacoeb, Agoes Mardiono
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 18 No. 2 (2015): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (516.648 KB) | DOI: 10.17844/jphpi.v18i2.10614

Abstract

Plants lindur (Bruguiera gymnorrhiza) is one of the plants that have a potential asa source of bioactive compounds for the antioxidant. This study aimed to determine thechemical composition, bioactive compound and determine antioxidant activity of leaves,bark and roots of plants lindur (B. gymnorrhiza). This research was conducted throughseveral stages proximate test, stratified extraction, phytochemical test, and antioxidantactivity test with DPPH. The highest yield value resulting by ethanol extract of the leavesis 12,85 % and the lowest was produced by n-hexane root extract that is 0,18 %. The leavescontain high levels of protein, fat, and water which is the higher of the two parts from theother samples. Bark containing ash content (4,12%) and carbohydrates (46,02%). Root hasthe highest levels of carbohydrates that is 25,91 %. Bioactive components were detected onethanol and ethyl acetate extracts of the leaves are flavonoids, tannins, phenols, saponins,steroids and tritepenoid. Bark extract ethanol, ethyl acetate and ethanol root bark hasbioactive components there are flavonoids, tannins, phenols, saponins, and tritepenoid. Ethyl acetate extract of the roots only have bioactive components there are flavonoids,phenols, saponins, and triterpenoids. The potential antioxidant activity produced fromethyl acetate extracts of bark lindur with IC50 values is 14,21 ppm.Keywords: Antioxidants, phytochemicals, roots, ethanol, Bruguiera gymnorrhiza
PERUBAHAN KOMPONEN SERAT RUMPUT LAUT CAULERPA SP. (DARI TUAL, MALUKU) AKIBAT PROSES PEREBUSAN Nurjanah, .; Jacoeb, Agoes Mardiono; Hidayat, Taufik; Chrystiawan, Rudy
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 10 No. 1 (2018): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (298.11 KB) | DOI: 10.29244/jitkt.v10i1.21545

Abstract

Rumput laut Caulerpa sp. dikenal sebagai sumber serat. Rumput laut Caulerpa sp. banyak ditemukan di pesisir pantai Indonesia, salah satunya di perairan Tual, Maluku Tenggara. Rumput laut ini sering dikonsumsi segar oleh masyarakat pesisir ataupun direbus terlebih dahulu. Perebusan bertujuan untuk menginaktifkan enzim dan menurunkan jumlah mikroba, namun dikhawatirkan dapat mempengaruhi komponen serat yang terkandung pada rumput laut. Penelitian ini bertujuan untuk menentukan perubahan kadar serat pangan (serat pangan larut dan tak larut), serat kasar, dan komponen serat (selulosa, hemiselulosa, lignin) akibat proses perebusan pada suhu 90ºC selama 5 menit. Analisis uji yang dilakukan, diantaranya analisis proksimat, kadar karbohidrat metode Luff schoorl, serat pangan metode enzimatis, serat kasar, dan komponen serat (selulosa, hemiselulosa, lignin). Hasil penelitian menunjukkan perebusan tidak mempengaruhi kadar lignin. Perebusan meningkatkan kadar serat pangan tak larut 1,25%, selulosa 13,91%, hemiselulosa 11,4%, dan menurunkan kadar serat pangan total 3,8%, serat pangan larut 5,05%, dan serat kasar 0,85%.
MINI-COI BARCODES SEBAGAI PENANDA MOLEKULER UNTUK KETERTELUSURAN LABEL PANGAN BERBAGAI PRODUK OLAHAN IKAN SIDAT Abdullah, Asadatun; Nurilmala, Mala; Sari, Aulia Sekar; Jacoeb, Agoes Mardiono
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 21 No. 2 (2018): Jurnal Pengolahan Hasil Perikanan Indonesia 21(2)
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.997 KB) | DOI: 10.17844/jphpi.v21i2.23507

Abstract

Ikan Sidat merupakan komoditas hasil perikanan penting dengan nilai ekonomis tinggi. DNA barcoding telah cukup banyak digunakan sebagai metode berbasis penentuan urutan nukelotida untuk mendeteksi jenis spesies pada ikan. Proses pengolahan dengan suhu tinggi dapat menyulitkan proses identifikasi berbasis penentuan urutan nukelotida. Tujuan penelitian ini adalah untuk mengidentifikasi spesies berbagai produk olahan ikan sidat menggunakan marka molekuler mini-COI barcodes. Pengolahan suhu tinggi yang digunakan pada penelitian ini adalah: panggang, penggorengan dalam minyak sayur, panggang, pengasapan panas dan pengolahan suhu sterilisasi. Seluruh sampel segar dapat teridentifikasi sebagai spesies Anguilla bicolor bicolor (99-100%) dan Anguilla marmorata (100%). Sampel olahan sidat berhasil diamplifikasi dengan marka mini-COI barcodes, sehingga dapat digunakan untuk aplikasi pengawasan label produk seafood secara rutin.
KANDUNGAN ASAM AMINO, TAURIN, MINERAL MAKRO-MIKRO, DAN VITAMIN B12 UBUR-UBUR (AURELIA AURITA) SEGAR DAN KERING -, Nurjanah -; Jacoeb, Agoes Mardiono; -, Nurokhmatunnisa -; Pujianti, Detti -
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 16 No. 1 (2013): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (379.532 KB) | DOI: 10.17844/jphpi.v16i1.8042

Abstract

Tujuan penelitian adalah menentukan komposisi gizi, asam amino, taurin, mineral makro dan mikro,dan vitamin B12 pada ubur-ubur (Aurelia aurita) segar dan kering. Asam amino esensial pada ubur-ubur,yaitu arginin, leusin, valin, treonin, lisin, isoleusin, fenilalanin, metionin, dan histidin, sedangkan asamamino non esensial, yaitu asam glutamat, glisin, asam aspartat, serin, alanin, dan tirosin. Asam aminoesensial tertinggi segar dan kering adalah arginin sebesar 1,72% (bk) dan 1,44% (bk) dan terendah histidin,yaitu sebesar 0,19% (bk) dan 0,13% (bk). Asam amino non esensial segar dan kering tertinggi adalah asamglutamat dan glisin, yaitu sebesar 3,26% (bk) dan 2,62% (bk) dan terkecil tirosin sebesar 0,38% (bk) dan0,41% (bk). Taurin segar sebesar 2,68% (bk) dan kering sebesar 0,67% (bk). Mineral makro tertinggi segardan kering adalah natrium, yaitu 180.092,1 ppm (bk) dan 111.209,4 ppm (bk), terkecil adalah kalsium, yaitu5.750,2 ppm (bk) dan 11,1 ppm (bk). Mineral mikro tertinggi segar dan kering adalah iodium, yaitu 8.291,5 ppm (bk)dan 1.800 ppm (bk) dan yang terkecil adalah tembaga yaitu 1,1 ppm (bk) dan 0,6 ppm (bk). Vitamin B12segar adalah 396,6 ?m/100 g (bk) dan kering 63,5 ?m/100 g (bk).Kata kunci: asam amino, mineral, taurin, ubur-ubur (Aurelia aurita), vitamin B
KANDUNGAN ASAM AMINO, TAURIN, MINERAL MAKRO-MIKRO, DAN VITAMIN B12 UBUR-UBUR (AURELIA AURITA) SEGAR DAN KERING Nurjanah, - -; Jacoeb, Agoes Mardiono; -, Nurokhmatunnisa -; Pujianti, Detti -
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 16 No. 2 (2013): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (380.169 KB) | DOI: 10.17844/jphpi.v16i2.8044

Abstract

Tujuan penelitian adalah menentukan komposisi gizi, asam amino, taurin, mineral makro dan mikro,dan vitamin B12 pada ubur-ubur (Aurelia aurita) segar dan kering. Asam amino esensial pada ubur-uburyaitu arginina, leusina, valina, treonina, lisina, isoleusina, fenilalanina, metionina, dan histidina, sedangkanasam amino non esensial yaitu asam glutamat, glisina, asam aspartat, serina, alanina, dan tirosina. Asamamino esensial tertinggi segar dan kering adalah arginina sebesar 1,72% (bk) dan 1,44% (bk) dan terendahhistidina yaitu sebesar 0,19% (bk) dan 0,13% (bk). Asam amino non esensial segar dan kering tertinggiadalah asam glutamat dan glisina yaitu sebesar 3,26% (bk) dan 2,62% (bk) dan terkecil tirosina sebesar0,38% (bk) dan 0,41% (bk). Taurin segar sebesar 2,68% (bk) dan kering sebesar 0,67% (bk). Mineral makrotertinggi segar dan kering adalah natrium yaitu 180.092,1 ppm (bk) dan 111.209,4 ppm (bk), terkecil adalahkalsium yaitu 5.750,2 ppm (bk) dan 11,1 ppm (bk). Mineral mikro tertinggi segar dan kering adalah iodium yaitu8.291,5 ppm (bk) dan 1.800 ppm (bk) dan yang terkecil adalah tembaga yaitu 1,1 ppm (bk) dan 0,6 ppm (bk).Vitamin B12 segar adalah 396,6 ?m/100 g (bk) dan kering 63,5 ?m/100 g (bk).Kata kunci: asam amino, mineral, taurin, ubur-ubur (Aurelia aurita), vitamin B12
IDENTIFICATION OF ANTIOXIDANT COMPOUNDS STRUCTURE LARGE-LEAFED MANGROVE FRUIT Sudirman, Sabri; Nurjanah, Nurjanah; Jacoeb, Agoes Mardiono
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 19 No. 2 (2016): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (541.187 KB) | DOI: 10.17844/jphpi.v19i2.13452

Abstract

Antioxidants are compounds that can inhibit or prevent the oxidation of the easily oxidized substrate.One of the plants as a potential source of bioactive compounds and antioxidant activity is large-leafedmangrove (Bruguiera gymnorrhiza). This plant is commonly found in the Pacific region of Southeast Asia,Ryukyu Islands, Micronesia and Polynesia (Samoa) to subtropical regions of Australia and has been usedby the society. This study aimed to determine the bioactive compounds structure of large-leafed mangrovehas the highest antioxidant activity. The compound structure prediction was done by Nuclear MagneticResonance (NMR). The compound structure in the selected antioxidant fractions are flavonol, glikosilfalvonand flavon. Those three compounds are flavonoid compound which has a great role as the one that hasantioxidant activity in large-leafed mangrove fruit.
HUBUNGAN PANJANG BOBOT DAN KEBIASAAN MAKAN IKAN BUNTAL PISANG (Tetraodon lunaris) DI PERAIRAN KABUPATEN CIREBON Pratama, Ginanjar; Nurjanah, Nurjanah; Suwandi, Ruddy; Jacoeb, Agoes Mardiono
Dinamika Maritim Vol 6 No 2 (2018): Dinamika Maritim, Vol. 6 No. 2, February 2018
Publisher : Coastal and Marine Resources Research Center, Raja Ali Haji Maritime University, Tanjungpinang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (614.793 KB)

Abstract

ORGANOLEPTIC CHARACTERISTICS AND CHEMICALS ILABULO CATFISH FORTIFICATION Harmain, Rita Marsuci; Dali, Faiza; Nurjanah, N.; Jacoeb, Agoes Mardiono
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 20 No. 2 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (169.308 KB) | DOI: 10.17844/jphpi.v20i2.18015

Abstract

Diversification of traditional food ilabulo made from raw catfish (Pangasius sp.) has the potential to be developed in Gorontalo province to substitute chicken viscera. The research aimed to make ilabulo substitute the raw material of chicken viscera with the catfish fortified Kappaphycus alvarezii seaweed and catfish bone meal and to determine the organoleptic and chemical characteristics of ilabulo catfish fortification. Fortified treatment is K. alvarezii  and catfish bone (5 dan 10% ),  B (10 and 15%) and  C (15 dan 20%). The organoleptic analysis used a hedonic scale of favorite criteria on the appearance, texture, color, flavor, and taste. The results of organoleptic analysis continued with Bayes test. The chemical analysis used the Association of Official Analytical Chemist method. The result of the hedonic characteristic of ilabulo catfish fortification was on the appearance  neutral criteria ? like (5.53?7.03), texture neutral criteria ? rather like (5.8?7.1), aroma rather like (6.3?6.73), color neutral ? like (6.1?7.03) and taste (6.07?6.53) neutral criteria ? rather like. The result of Bayes test obtained by a texture of importance value 5, appearance importance value 5, aroma of importance value 4, color of interest value 3 and taste of importance value 2. Characteristic of ilabulo culture of selected fortified catfish that was the fortification of K. alvarezii seaweed 15% and catfish  bone flour (20%) (fortification C) obtained by water content 56.46%, ash 11.54%, protein 7.78%, fat 8.91%, coarse fiber 0.61%, carbohydrate 22.07% and calcium 0.315 %.