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POLIMORFISME G.-371T>A PROMOTOR GEN MIOSTATIN PADA SAPI PEDAGING INDONESIA Sutikno, Sutikno; Priyanto, Rudy; Sumantri, Cece; Jakaria, Jakaria
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 2 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (746.116 KB) | DOI: 10.18343/jipi.25.2.239

Abstract

Myostatin (MSTN) gene acts as a negative regulator of muscle growth. The aim of the present study was to identify polymorphism of g.-371T>A in promoter region of MSTN gene in Indonesian beef cattle. Blood samples were collected from 191 cattle, including Bali (BL) (42), Madura (MD) (20), Pesisir (PI) (17), Katingan (KT) (16), Ongole grade (PO) (22), Pasundan (PD) (14), Sumba ongole (SO) (10), Brahman (BH) (17), Simmental (SM) (15), and Limousin (LM) (18). Polymorphism of MSTN gene was analyzed using PCR-RFLP (DraI) and direct sequencing methods. Results of genotyping g-371T>A were polymorphic (TT, TA, and AA genotypes) in Simmental, SO, and Katingan. The frequencies of alleles T and A were 0.83; 0.90; 0.97 and 0.17; 0.10; 0.03 respectively. The values of Ho and He were 0.06?0.20 and 0.06?0.28, respectively. The results of this study are in Hardy-Weinberg equilibrium (P> 0.05). Whereas in PD, MD, PI, PO, BH, LM, and BL were monomorphic (TT genotype). The result of sequencing the promoter region of MSTN gene found that mutations transversion was occurred in T to A at g.-371. It was concluded that g.-371T>A of MSTN gene was polymorphic which was potential to be used as genetic markers of muscle growth in SM, SO, and KT cattle. Keywords: SNP g.-371T>A of MSTN gene, Sumba ongole cattle, Katingan cattle, Simmental cattle
POLYMORPHISM OF MYOSTATIN (MSTN) PROMOTER GENE AND ITS ASSOCIATION WITH GROWTH AND MUSCLING TRAITS IN BALI CATTLE Khasanah, Himmatul; Gunawan, A.; Priyanto, R.; Ulum, M. F.; Jakaria, Jakaria
Media Peternakan Vol. 39 No. 2 (2016): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (976.226 KB) | DOI: 10.5398/medpet.2016.39.2.95

Abstract

Myostatin (MSTN) gene plays a key role in skletal muscle homeostasis such as inducing muscle athrophy, poliferation of myoblast, increasing ubiquitin-proteasomal, downregulating IGF pathway, and glucolysis. Myostatin gene expression is controled by CpG island located in promoter region. The objectives of this research were to identify polymorphism of MSTN promoter gene and to associate the polymorphism of SNP with growth and muscling traits in Bali cattle. A total of 48 Bali cattle from BPTU-HMT Bali island was screened to identify genetic polymorphisms in MSTN promoter region using sequencing method. The growth and muscling traits were measured at 12 months of age. The muscling traits were evaluated using ultrasound console with linear transducer having frequency 6.5 Hz and scaning we conducted at 130 mm in deep. Analysis of polymorphism was conducted by using PopGen 1.32 software. The association of MSTN with growth and muscling traits were analyzed by using General Linear Model (GLM) procedure. This result showed that a total of 20 polymorphic SNPs (seven SNPs in CpG island) were detected in this region. Although, only 3 SNPs (g.-8078C>T, g.-7996G>C, and g.-7930A>G) had equilibrium condition in Hardy-Weinberg analysis. The association result showed that 2 SNPs (g.-7799T>C and g.-7941C>T) were significantly associated with intramuscular fat percentage (P?0.05) in Bali cattle. Although the 2 SNPs were nominally significant at nominal P?0.05 threshold, they were not significant after Bonferroni correction for multiple testing. It could be concluded that MSTN promoter gene was polymorphic in Bali cattle and there were 2 SNPs associated with carcass quality.
KERAGAMAN SIFAT KUALITATIF PADA SAPI SILANGAN PO DAN BELGIAN BLUE MENGGUNAKAN ANALISIS KOMPONEN UTAMA Jakaria, Jakaria; Zulkipli, Fuadi; Edwar, Edwar; Ulum, Mokhamad Fakhrul; Priyanto, Rudy
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 7, No 1 (2020): JITRO, Januari
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jitro.v7i1.8675

Abstract

The purpose of this study was to determine the diversity of qualitative traits in PO, Belgian Blue and PO-Belgian Blue crosses using principal component analysis. The total samples of cattle used were 36 consisting of 8 heads Belgian Blue (4 males and 4 females), 17 heads Belgian PO-Belgian cross-breed (11 males and 6 females) and 11 PO heads (all females)). The diversity of qualitative traits observed were 17 characteristics namely plain body-color (WTP), combination body-color (WTK), horned (MT), humped (MP), sagging (MG), black tail tip color (WUEH), white tail tip color (WUEH), black eyelash color (WBMH), white eyelash color (WBMP), circle hair head (PRK), circle hair back (PRP), circle hair bracket (PRB), black nail color (WKH), nail color white (WKP), double muscle (PG), black muzzle (WMH) and white muzzle (WMP). Qualitative data were analyzed descriptively by calculating the proportion value, while the determinant variables of each breed were analyzed using the principal component analysis method (AKU) with the Minitab version 7 program. The results showed that the characteristics of qualitative traits in PO, Belgian Blue, and PO-Belgian Blue cross-breed cattle have a high diversity. PO and Belgian Blue cattle breeds can be clearly distinguished based on the characteristics of qualitative traits, whereas PO-Belgian Blue crossbred cattle have clusters that are close to PO cattle clusters. Found character traits for each cattle breed in both PO, Belgian Blue, and PO-Belgian Blue cross-breed. There are three characteristics of qualitative traits possessed in each cow nation, namely the variable circle hair head (PRK), circle hair back (PRP) and horned characters (MT). Based on the results obtained that the qualitative traits in cattle can be used as a distinguishing character between PO, Belgian Blue, and PO-Blue Belgian cross cattle breeds.
EVALUASI KINERJA PERTUMBUHAN SAPI SILANGAN BELGIAN BLUE DAN PERANAKAN ONGOLE Jakaria, Jakaria; Edwar, Edwar; Ulum, Mokhamad Fakhrul; Priyanto, Rudy
Jurnal Agripet Vol 19, No 2 (2019): Volume 19, No. 2, Oktober 2019
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v19i2.15022

Abstract

ABSTRAK. Tujuan penelitian ini adalah mengevaluasi kinerja pertumbuhan sapi persilangan Belgian Blue (BB) dan sapi Peranakan Ongole (PO) pada turunan pertama (F1). Sapi persilangan (BB dan PO) yang digunakan sebanyak 15 ekor terdiri atas jantan 6 ekor dan betina 9 ekor, selain itu digunakan 8 ekor sapi PO betina yang dipelihara di Balai Embrio Ternak (BET) Cipelang Bogor. Peubah yang diamati adalah bobot lahir (BW), bobot sapih (WW) 205 hari, bobot 1 tahun (YW), pertambahan bobot badan harian dari lahir (GBW) sampai sapih (GWW) dan pertambahan bobot badan harian dari sapih (GWW) sampai umur 1 tahun (GYW). Data dianalisis secara deskriptif dan perbedaan antar sapi silangan (BB-PO) dengan sapi PO dilakukan uji t. Hasil analisis bobot badan dan pertumbuhan menunjukkan bahwa, bobot badan sapi silangan BB dan PO lebih tinggi dibandingkan dengan sapi PO pada WW 205 hari (165,2±21,3 kg dan 115±15,2 kg), YW (365 hari) (240,7±28,1 kg dan 194,9±26,1 kg) dan GBW sampai GWW (0,675±0,097 kg dan 0,441±0,059 kg). BW dan GWW sampai GYW tidak berbeda antara sapi silangan BB-PO dengan PO. Hasil penelitian ini menunjukkan bahwa program cross-breeding sapi BB dengan PO dapat meningkatkan kinerja bobot badan dan pertumbuhan.  (Growth performance evaluation of belgian blue and ongole crossbreed) ABSTRACT. The purpose of this study was to evaluate the growth performance of cross-breeding of Belgian Blue (BB) and Ongole Grade (PO) cattle in the first generation (F1). Crossbred of BB and PO cattle used as many as 15 individuals consisting of 6 males and 9 females, also, 8 individual PO females are used which are kept in the Livestock Embryo Center (BET) Cipelang, Bogor. The observed variables were birth weight (BW), weaning weight (WW) 205 days, 1-year weight (YW) 365 days, daily body weight gain from birth (GBW) to weaning (GWW) and daily body weight gain from weaning (GWW) until the age of 1 year (GYW). Data were analyzed descriptively and the difference between cross-breeding (BB-PO) cattle and PO cattle was analyzed by t-test. The results of body weight and growth analysis showed that crossbred BB and PO cattle were higher than PO cattle at WW 205 days (165.2 ± 21.3 kg and 115 ± 15.2 kg), YW (365 days) (240.7 ± 28.1 kg and 194.9 ± 26.1 kg ) and GBW to GWW (0.675 ± 0.097 kg and 0.441 ± 0.059 kg). BW and GWW to GYW do not differ between crossbred BB-PO and PO cattle. The results of this study indicate that the cross-breeding program for BB cattle with PO cattle breeds can improve the performance of body weight and growth.
IDENTIFICATION OF A SINGLE NUCLEOTIDE POLYMORPHISM AT HINF-1 ENZYME RESTRICTION SITE OF PIT-1 GENE ON INDONESIAN BALI CATTLE POPULATION Jakaria, Jakaria; Noor, Ronny R
Media Peternakan Vol. 38 No. 2 (2015): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (626.523 KB) | DOI: 10.5398/medpet.2015.38.2.104

Abstract

This study aimed to determine the Pit-1|Hinf-1 gene polymorphism in Bali cattle (Bos javanicus) as Indonesian native cattle and besides Madura, Pesisir, Aceh, and Katingan cattle breeds as a comparison. DNA samples were extracted from 488 blood samples consisting of Bali (245 heads), Madura (68 heads), Aceh (25 heads), Pesisir (100 heads) and Katingan (50 heads) cattle. The diversity of the Pit-1|Hinf-1 gene wasere analyzed using PCR-RFLP. Whereas the nucleotide base mutations were identified by sequencing. Genotyping data were analyzed by calculating the allele frequency, observed heterozygosity (Ho) and expected heterozygosity (He) values as well as Hardry-Weinberg equilibrium test using POPGENE 1.31 program. Whereas, Tthe sequence data were analyzed by using MEGA6 program. The Pit-1|Hinf-1 gene fragment analysis showed that Bali, Madura, Pesisir, Aceh, and Katingan cattle had high BB genotype, resulting in B allele frequency of 0.982, 0.963, 0.925, 0.960, and 0.960, respectively. Ho and He values were 0.074-0.130 and 0.036-0.139, respectively. Hardy-Weinberg equilibrium test did not significant for all breed populations, except for Aceh cattle population (P<0.05). Mutation from guanine (G) to adenine (A) was found in Pit-1 gene fragment. Therefore, Pit-1|Hinf-1 gene fragment had low genetic diversity in Bali cattle and other breeds population.Key words: Bali cattle, Pit-1|Hinf-1 gene, polymorphism
BERAKHLAKLAH KAMU DENGAN AKHLAK ALLAH Jakaria, Jakaria
Jurnal Pendidikan Karakter JAWARA (Jujur, Adil, Wibawa, Amanah, Religius, Akuntabel) Vol 1, No 2 (2015): Jurnal Pendidikan Karakter “JAWARA” (JPKJ)
Publisher : Jurnal Pendidikan Karakter JAWARA (Jujur, Adil, Wibawa, Amanah, Religius, Akuntabel)

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Abstract

Penelitian ini bertujuan untuk (1) mendeskripsikan manfaat dongeng dalam pembentukankarrakter anak dan (2) mendeskripsikan teknik penyampaian dongeng yang sesuai dengan usiaanak. Fokus penelitian ini adalah “Bagaimana pembentukan karakter anak?” selanjutnya focuspenelitian ini dirinci menjadi dua subfokus, yaitu (1) Bagaimana manfaat dongeng dalampembentukan karakter anak? Dan (2) Bagaimana teknik penyampaian dongeng yang sesuai denganusia anak? Penelitian ini menggunakan metode kualitatif kajian pustaka dengan teknik analisisdata isi. Sumber data penelitian ini adalah buku-buku yang membicarakan pendidikan karakter,psikologi kepribadian, dan karya sastra. Kesimpulan penelitian ini adalah (1) Dongeng mempunyaibanyak manfaat, di antaranya adalah (a) dongeng mengajarkan nilai moral yang baik, (b) dongengmengembangkan daya imajinasi anak, (c) dongeng menambah wawasan anak, (d) dongengmeningkatkan kreativitas anak, (e) dongeng mendekatkan anak dengan orang tua, dan (f) dongengmenghilangkan ketegangan (stress). (2) Ada beberapa cara (teknik) yang dapat dilakukan dalammenyampaikan dongeng yang sesui dengan usia ana, yaitu (a) anak-anak diminta menyebutkanciri-ciri tokoh dalam dongeng, (b) anak-anak diminta mengaitkan dongeng dengan lagu tertentu,(c) orang tua/guru menceritakan fakta yang terkait dengan tokoh dongeng, dan (d) orang tua/gurumemberi kebebasan kepada anak untuk membuat akhir cerita dongeng. Oleh karena itu, orang tuadan guru sebaiknya menyisihkan waktu untuk memberikan dongeng yang mendidik anak-anakkarena dongeng mempunyai potensi konstruksi untuk mendukung perkembangan mental anak.Selain itu, orang tua dan guru sebaiknya mempunyai keterampilan dalam mendongeng. Orang tuadan guru harus menguasai cara (teknik) penyampaian dongeng yang sesuai dengan usia anaksehingga dongeng yang disampaikan menjadi sangat menarik bagi anak-anak.Kata kunci: Pembentukan karakter, manfaat dongeng, dan teknik penyampaian Dongeng.
Application of Linear Body Measurements for Predicting Weaning and Yearling Weight of Bali Cattle Gunawan, A; Jakaria, Jakaria
ANIMAL PRODUCTION Vol 12, No 3 (2010): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (339.875 KB)

Abstract

The objective of this research was to predict the weaning and yearling weight of Bali cattle using simple linear body measurement. The height wither (HW), body length (BL) and heart girth (HG) were measured in centimeters, using caliper, whereas weaning (WW)and yearling weight (YW) was determined in kilograms using a weighing scale. Results of the correlation coefficient showed  that hearth girth (HG) highly correlated with weaning and yearling weight were 0.847 and 0.871 respectively. In all, the height wither (HW) had the least correlation coefficient were 0.328 and 0.782 on weaning and yearling weight respectively.  Results of the stepwise regression showed that HG was a good estimator of WW and YW of Bali cattle followed by HW and BL. The comparison of residuals indicated that only two equations (HG and BL) accurately predicted weaning and yearling weight. From these results, it was concluded that weaning and yearling weight of Bali cattle can be estimated using simple linear body measurement of heart girth, body length and height wither especially in most remote areas where farmers are challenged with the unavailability of weighing scale. (Animal Production 12(3): 163-168 (2010)Key Words: weaning and yearling weight, linear body measurements, Bali cattle
KONTRIBUSI PENDIDIKAN KARAKTER TERHADAP PEMBANGUNAN KARAKTER BANGSA Jakaria, Jakaria
Jurnal Pendidikan Karakter JAWARA (Jujur, Adil, Wibawa, Amanah, Religius, Akuntabel) Vol 2, No 2 (2016): Jurnal Pendidikan Karakter “JAWARA” (JPKJ)
Publisher : Jurnal Pendidikan Karakter JAWARA (Jujur, Adil, Wibawa, Amanah, Religius, Akuntabel)

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Abstract

Pendidikan karakter dewasa ini, telah menjadi perhatian banyak pihak, mengingat banyaknyafenomena social dalam kurun waktu sekarang ini sangat memperihatinkan. hal ini ini kita bisa lihatdari sumber media, tontonan tindakan kekerasan, kerusuhan dan dekadensi moral telah menjadisuguhan setiap hari bahkan dari hari ke hari kuantitas jumlah kasus semakin tak terbendung. olehkarena itu pendidikan karakter dapat dianggap sebagai salah satu solusi untuk mengurangiproblematika bangsa saat ini, untuk menjadi bangsa yang lebih baik, sehingga nantinya lahirgenerasi bangsa yang tumbuh berkembang dengan karakter yang bernafas nilai-nilai luhur bangsaserta agama.Kata kunci: Pendidikan Karakter, Generasi Bangsa, Pembangunan.
The Ghrelin Receptor (Ghsr) Gene Polymorphism in Indonesian Local Chicken and Crossbreed Is Associated With Carcass Traits Khaerunnisa, Isyana; Jakaria, Jakaria; Arief, Irma Isnafia; Budiman, Cahyo; Sumantri, Cece
ANIMAL PRODUCTION Vol 19, No 2 (2017)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (943.521 KB) | DOI: 10.20884/1.jap.2017.19.2.596

Abstract

Ghrelin receptor (GHSR) gene is candidate gene for growth performance in chicken by modulating growth hormone release from the pituitary by binding to its ligand of ghrelin. Ghrelin gene, or growth hormone secretagogue (GHS) gene, is well known as feed intake and energy homeostasis regulator in mammals and birds. The objectives of this study were to identify the polymorphism of the T1857C GHSR locus in Indonesian local chicken and to evaluate its effects on carcass traits. The gene polymorphism was identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using Hin6I restriction enzyme. Effect of genotype on carcass composition was analyzed using SAS General Linear Model (GLM) procedure. The genotyping was performed on 343 individuals including Merawang, Pelung, Sentul, Kampung, broiler (strain Cobb, parent stock), F1 crossbred of Kampung x broiler (strain Cobb, parent stock), and F2 crossbred of Kampung x broiler (strain Cobb, parent stock). All individuals were successfully amplified and were resulted in a 470 bp PCR product. This locus was polymorphic with two alleles (T and C) and three genotypes (TT, CT, and CC). The T allele and TT genotype were predominant in all populations. Individuals with CT genotype were significantly had higher live weight at 26w, carcass weight, commercial cuts weights, and muscles weights than TT genotype in F2 crossbred of Kampung x broiler population. Association of the T1857C GHSR locus-polymorphism with chicken carcass composition has been described in Indonesian chicken, providing evidence that GHSR might be an important candidate gene for chicken carcass traits.
APLIKASI PROGRAMMABLE LOGIC CONTROLLER (PLC) PADA SISTEM KONTROL PROSES PENGELASAN INNER DAN OUTER TABUNG IRADIASI W, I Wayan W; Sori, Sofyan; Jakaria, Jakaria; Heru W, Artadi; Mulyono, Mulyono
Jurnal Forum Nuklir JFN Vol 4 No 1 Mei 2010
Publisher : BATAN

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Abstract

Pada proses pengelasan inner dan outer tabung iradiasi, diperlukan sumber daya manusia yang handal sehingga hasil pengelasan memenuhi persyaratan yang ditetapkan untuk dapat diiradiasi di reaktor nuklir. Karena adanya keterbatasan sumber daya manusia yang mampu melakukan pengelasan tabung iradiasi secara sempurna, maka dibuat sistem kontrol proses pengelasan inner dan outer tabung iradiasi. Dengan sistem kontrol tersebut maka pengelasan tabung iradiasi dapat dilakukan secara otomatis. Metoda yang digunakan yaitu dengan menggunakan Programmable Logic Controller (PLC) yang difungsikan untuk mengatur proses pengelasan secara otomatis melalui program yang ditanamkan di dalamnya. Hasil yang diperoleh berupa unit kontrol yang telah diuji secara simulasi, kemudian dirangkai pada unit mesin las, dan selanjutnya dilakukan uji pengelasan terhadap tabung iradiasi. Pada hasil pengelasan dilakukan juga uji kebocoran dengan metode bubble test. Sistem kontrol dapat dinyatakan berfungsi dengan baik, karena semua langkah dalam proses pengelasan sudah terpenuhi. Hasil pengelasan yang kurang maksimal lebih besar diakibatkan oleh kondisi tabung iradiasi yang kurang sempurna seperti bentuk kurang simetris dan permukaan tidak bersih, serta kondisi bagian mekanik pada pemutar tabung iradiasi yang tidak presisi.