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AKTIFITAS ANTI BAKTERI SPESIES ASTERIAS FORBESII TERHADAP BEBERAPA JENIS BAKTERI PATOGEN Juariah, Siti; Suryanto, Dwi; Jamilah, It
Berkala Perikanan Terubuk Vol 42, No 2 (2014): Juli 2014
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.043 KB) | DOI: 10.31258/terubuk.42.2.%p

Abstract

ABSTRACTTo avoid contamination of bacteria pathogens natural and safe antibacterial agent is needed. The alternative sources of antibacterial compound is derived from sea star. In this extraction study of sea star activity showed that activity of the methanol from extract of starfish have the highest inhibition against for several bacterial pathogens compared to that of ethyl acetate and n-hexane extract with inhibition zone of 9.5 mm, 8.5 mm, 10.0 mm, 11.0 mm in S. aureus, B. subtilis, P. auroginosa and E. coli respectively. It showed that of TLC preparatif triterpenoid was capable inhibition more in Gram negative bacteria (Asterias forbesii) has been caried out to obtain antibacterial compounds. To determine bioactive components in the extract of sea star chemical test has been conducted. Toxicity of secondary metabolites was determind using method of Brine Shrimp Lethality Test (BSLT). Assay of antibacterial activity of starfish extract was conducted against four pathogenic bacterial strains, two Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis), and two Gram-negative bacteria (Pseudomonas auroginosa and Escherichia coli). The result showed that the methanol extract contained alkaloid, terpenoids, saponins and flavonoids, while the extract of n-hexane and ethyl acetate only contain saponins and flavonoids. BSLT test showed that LC50 of extract of n-hexane, ethyl acetate and methanol were 1412,54 ppm, 13182,57 ppm and 63,10 ppm respectively. Antibacterial (E. coli and P. auroginosa) bacteria compared with Gram positive (S. aureus and B. subtilis) .Keywords: Asterias forbesii, Staphylococcus aureus, Bacillus subtilis, Pseudomonas auroginosa, Escherichia coli, antibacterial compound
ISOLASI BAKTERI PATOGEN OPORTUNISTIK DARI TAMBAK UDANG SUMATERA UTARA Wahyuni, Yusnita; Jamilah, It; Suryanto, Dwi
Jurnal AGROHITA Vol 1, No 2 (2017): Agrohita Vol 1 Nomor 2 Tahun 2017
Publisher : Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan

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Abstract

Bakteri patogen oportunistik merupakan bakteri yang secara alami bukan berada di habitat suatu lingkungan tetapi masuk akibat tercemarnya lingkungan tersebut dengan limbah manusia. Keberadaan bakteri patogen oportunistik di tambak udang disebabkan karena tercemarnya air tambak oleh limbah industri maupun domestik. Penelitian ini bertujuan untuk mengetahui keberadaan bakteri patogen oportunistik pada tambak udang di Sumatera Utara (Percut, Pantai Labu, Pantai Cermin). Bakteri patogen oportunistik diisolasi dari usus udang, sedimen dan air tambak udang dengan menggunakan media selektif/diferensial. Dari hasil isolasi ditemukan adanya bakteri Escherichia coli, Salmonella sp., dan Staphylococcus sp. Jumlah tertinggi dari ketiga bakteri tersebut ditemukan pada sampel yang berasal dari daerah Percut.
Aktivitas Antimikroba Isolat Bakteri Asam Laktat yang Diisolasi dari Pangan Pliek U terhadap Bakteri Staphylococcus aureus, Escherichia coli dan Khamir Candida albicans secara in Vitro Kiti, Annisa Ammalia; Jamilah, It; Rusmarilin, Herla
JOURNAL OF HEALTHCARE TECHNOLOGY AND MEDICINE Vol 4, No 1 (2018): April 2018
Publisher : Universitas Ubudiyah Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33143/jhtm.v4i1.174

Abstract

Bakteri asam laktat (BAL) telah diisolasi dari pangan khas Aceh pliek u. Penelitian ini bertujuan untuk mengetahui daya hambat dan menguji aktivitas antimikroba BAL terpilih yang diisolasi dari pliek u terhadap bakteri Staphylococcus aureus, Escherichia coli dan khamir Candida albicans. Hasil pengujian menunjukkan bahwa isolat BAL terpilih dapat menghambat pertumbuhan kedua bakteri uji, namun tidak dapat menghambat C. albicans. Hal ini ditunjukkan oleh terbentuknya zona bening di sekeliling isolat BAL terpilih. Zona hambat terbesar terhadap bakteri Staph. aureus dan E. coli  berturut-turut yaitu ditunjukkan oleh isolat SP3 sebesar 7.7 mm dan SP5 sebesar 28 mm, sedangkan zona hambat terendah berturut-turut ditunjukkan oleh isolat SP4 dan SP5 sebesar 0.25 mm dan SP2 sebesar 1.5 mm.Kata kunci : pliek u, bakteri asam laktat, bakteri patogen, aktivitas antimikroba
Ligninolytic Activity of Fungi Isolated from Empty Fruit Bunch of Oil Palm (Elaesis guineensis Jacq.) Sitompul, Sepwin Nosten; Munir, Erman; Jamilah, It
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 36, No 2 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2019.36.2.967

Abstract

Lignin is a natural polymer and plays an important role as a compound of plant cell wall constituent. A study about the degradation of lignin in the environment has been receiving considerable attention because the complex structure and difficult to be degraded compared to the degradation of others plant cell wall constituent. A study to determine the activity of the ligninolytic enzyme (lignin peroxidase, manganese peroxidase and laccase) of fungi isolated from oil palm empty fruit bunch. This study has been done with a screening of ligninolytic activity using potato dextrose agar supplemented with tannic acid (0,1%), showed that two out of five fungal isolates have ligninolytic activity. The highest activity of lignin peroxidase was produced by SN2 isolatesi.e. 9.677U ml-1, whereas the highest activity of manganese peroxidase and laccase was produced by SN3isolates i.e.1.942 U ml-1 and 1.846 U m-1 respectively.
SELEKSI BAKTERI PENAMBAT NITROGEN DAN PENGHASIL HORMON IAA (INDOLE ACETIC ACID) DARI RIZOSFER TANAH PERKEBUNAN KEDELAI (GLYCINE MAX L.) Tarigan, Ratna Sari; Jamilah, It; D, Elimasni
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

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Abstract

Rizosphere bacteria can be used to fix nitrogen and  produce IAA (Indol Acetic Acid) as biofertilizer to support plant growth. The ability of rhizosphere bacteria can be improved using biotechnology. The aim of this study is to select the most potential nitrogen fixing and IAA producing bacteria. Nitrogen fixing and IAA producing bacteria were isolated using JNFB and Luria Bertani + L-tryptofan medium respectively. The ability of nitrogen fixing bacteria were tested by ARA method, while the ability of IAA producing bacteria were investigated by spectrophotometer technique of 535 nm. The highest IAA concentration was produced by isolate I3 which was 33.3 ppm and the highest concentration of nitrogen was yielded by isolate N3 which was 29.93 ppm. Both N3 isolate and I3 isolate potentially as biofertilizer known as PGPR (Plant Growth Promoting Rhizobacteria).   Keywords: nitrogen fixing bacteria, IAA producing bacteria, PGPR
PENGENDALIAN SEL BIOFILM BAKTERI PATOGEN OPORTUNISTIK DENGAN PANAS DAN KLORIN Silitonga, Yusnita Wahyuni; Jamilah, It; Suryanto, Dwi
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

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Abstract

Opportunistic pathogenic bacteria are bacteria that are naturally not present in an environment that, but it dues to contamination of the environment by human waste. From previous research it was found E. coli, Staphylococcus sp. and Salmonella were opportunistic pathogens of the shrimp aquaculture (Percut, Pantai Labu, Pantai Cermin). The aims of this study is to know the ability of these bacteria to form biofilms as well as its control using chlorine and heat. In order to test the ability of the bacteria to form biofilms, the stainless steel have been soaked in SWC media for 1, 3, 6 days. E. coli, Salmonella, and Staphylococcus sp. may form biofilms on incubation of the 1st day but the highest growth was on the 6th day of incubation by E. coli with a 6,35 x 104 CFU/SS, whereas of the lowest biofilm number was found in Salmonella with a 0,28 x 104 CFU/SS. The number of biofilm cells grow in line with a length of incubation. In this research, the most effective concentration of chlorine to kill biofilm cell was  225 ppm for 2 minutes and heat was 100 0C for 5 minutes. The higher the concentration of chlorine and the temperature given more effective to kill the bacteria.   Keyword: biofilm, chlorine, heat, opportunistic, stainless steel
VIABILITAS DAN KERIAP BACILLUS SP. BK17 DAN ENTEROBACTER SP. BK15 PADA SUMBER KARBON DAN NITROGEN YANG BERBEDA Nasrah, Sirma Novita; Suryanto, Dwi; Jamilah, It
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

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Abstract

To propagate bacterial cell for biocontrol purpose, suitable nutrient have to be determined in which carbon and nitrogen source was often as limited factor of bacterial growth. Proper storage for biocontrol agent such as bacterial cell should also be considered in order to keep the cell viable when used. The aim of this study is to find out suitable carbon and nitrogen sources for viability and swarming of chitinolitic bacterial Bacillus sp. BK17 and Enterobacter sp. BK15. The highest population of bacterial growth (3.7x108 cfu/ml) was found in molases-sodium nitrate (MS) medium and the lowest population was found in crab shell-sodium nitrate (CS) growth (2.4x108 cfu/ml) after 25 days of incubation. The swarming activity of the isolates were varied to some extent with the highest was 51 mm in 2% agar molases-urea after 5 days of incubation. Molases-sodium nitrate (MS) medium is suitable carbon and nitrogen source for the viability of Bacillus sp. and Enterobacter sp. Meanwhile, agar molases-urea medium with 2% agar is suitable medium for swarming ability for both bacteria. Keywords : Bacillus, C and N-source, Enterobacter, swarming, viability