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DETEKSI MRSA (METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS) PADA PASIEN RUMAH SAKIT DENGAN METODE MALDI-TOF MS DAN MULTIPLEX PCR Kemalaputri, Dian Wahyu; Jannah, Siti Nur; Budiharjo, Anto
Jurnal Akademika Biologi Vol. 6 No. 4 Oktober 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Methicillin Resistant Staplylococcus aureus (MRSA) is a strain of Staphylococcus aureus which has been resistant to methicillin antibiotics and ?-lactam group. The mechanism of MRSA resistance occurs because the Staphylococcus aureus produces Penicillin Binding Protein (PBP2a) encoded by the mecA gene that has low affinity to methicillin. Nowadays, MRSA is tested by resistance test using Oxacillin 1 ug. This method requires pure isolate and bacterial culture, so that the results can be seen in 5 days. To find a rapid and precise diagnostic technique for detecting MRSA, mecA gene detection utilizing PCR multiplex technique as the alternative diagnostics. This study aims to find a quick and precise alternative diagnostic technique for MRSA examination, which is utilizing MALDI-TOF MS and multiplex PCR technique.Keywords: MRSA, Staplylococcus aureus, MALDI-TOF MS, Multiplex PCR.
PERAN HUMAS DALAM MENGEMBANGKAN PENDIDIKAN MELALUI OTONOMI PENDIDIKAN TINGGI Jannah, Siti Nur
Islamic Management: Jurnal Manajemen Pendidikan Islam Vol 3, No 01 (2020)
Publisher : Sekolah Tinggi Agama Islam Al Hidayah Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30868/im.v3i01.268

Abstract

Quality management at the moment is a phenomenon that requires special attention for a college, especially private colleges. This means that private universities, especially private universities in the city of Bandung to always be directed at improving quality activities. Because otherwise the private university lacks a place in the hearts of society that ultimately threatens its existence. A publicist is the bridge or the first path of an image that is formed in the community at a particular institution. The importance of applying this public relations strategy in marketing universities in every institution that requires the performance of a publicist to help the course of a work program, as well as public relations can maintain the image of an institution in the institution, so that an institution can be recognized and trusted by public relations strategy. Thus the focus of this study is the quality management of universities related to the study of the influence of visionary leadership and lecturer performance on the quality of the university.
ISOLASI BAKTERIOFAG SALMONELLA SPP. DARI BIOFILM PADA SISTEM AIR MINUM ISI ULANG Damayanti, Rahayu; Jannah, Siti Nur; wijanarka, w; Rahaju, Sri Hartin
Jurnal Akademika Biologi Vol. 5 No. 2 April 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The public demands for the refill drinking water increases causing the development of refill drinking water industries. However, the problems occurred is no standard method to process drinking water with sterile and lack of government oversight. These cases give rise to sanitation which is the formation of bacteria pathogen forming biofilms in refill drinking water system. One of the bacteria pathogens is Salmonella. Salmonella in refill drinking water can cause diarrhea, because it can produce cytotoxin and enterotoxin. Bacteriophages are viruses that infect bacteria. This study aims to find natural isolates of bacteriophage from biofilm samples to infect Salmonella spp. in refill drinking water system. The isolates obtained is then characterized by biochemical test including Gram stain, a test Kligler Iron Agar (KIA) and api assay 20 E. The positive Salmonella spp. isolates are in the second dilution refill drinking water depot. The isolation of bacteriophage from biofilm is conducted with bacteriophages amplification and bacteriophage filtrate. The Infection test is performed by using Salmonella enterica, Salmonella 7A1 from Teluk Ambon and Salmonella spp. from refill drinking water depot. Platting is performed on serial dilutions of 10-2 to 10-10phage dilution. Positive result is characterized by the formation of plaque which is in source water samples, water product and drinking water depot. The number of plaques formed is calculated by Plaque Forming Units (PFU/mL) to determine quantification or calculation phages.
DETEKSI GEN TLH DAN TDH PADA BAKTERI VIBRIO PARAHAEMOLYTICUS DARI AIR TAMBAK UDANG VANNAME (LITOPENAEUS VANNAMEI) DI KABUPATEN REMBANG Hasrimi, Adila Nawan; Budiharjo, Anto; Jannah, Siti Nur
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Vibrio parahaemolyticus is hallophilic gram-negative bacteria that live as natural inhabitant in aquatic environment. All Vibrio parahaemolyticus strain known to have thermolabile hemolysin encoded by tlh gene as species marker. Thermostable direct hemolysin encoded by tdh gene is responsible for regulating one of the virulence factors in Vibrio parhaemolyticus. The aim of this research is to detect tlh gene and tdh gene from water of vanname shrimp?s aquaculture in Rembang regency. Colonies of green-blueish bacteria grew from the isolation of  vanname shrimp?s aquaculture water in CD-VP media which is identified as Vibrio parahaemolyticus. The isolated bacteria is specifically identified as Vibrio parahaemolyticus bacteria by the detection of tlh gene. Molecular analysis shows tdh negative result that indicates tdh gene is not present in the isolated bacteria. Vibrio parahaemolyticus isolate were cultured in Wagatsuma agar for the tdh gene confirmation test that showed Kanagawa negative result, in which indicated that V. parahaemolyticus did not produce thermostable direct hemolysin. Vibrio parahaemolyticus isolate did not show any virulence factors to initiate host colonization in the aquatic environment. Keywords: Vibrio parahaemolyticus, tdh gene, tlh gene
CHARACTERISTICS OF LACTIC ACID BACTERIA ISOLATED FROM GASTROINTESTINAL TRACT OF CEMANI CHICKEN AND THEIR POTENTIAL USE AS PROBIOTICS Jannah, Siti Nur; Dinoto, Achmad; Wiryawan, Komang Gede; Rusmana, Iman
Media Peternakan Vol. 37 No. 3 (2014): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5594.722 KB) | DOI: 10.5398/medpet.2014.37.3.182

Abstract

The aims of this study were to screen and characterize lactic acid bacteria (LAB) isolated from gastrointestinal (GI) tract of Cemani chicken, one of Indonesian local chicken and to investigate their potential use as probiotics. LAB were isolated from GI tract using MRSA and GYPA media and incubated anaerobically. Selected LAB were determined their probiotic properties with several assays. Identification of selected LAB was based on 16S rDNA sequences, morphological and biochemical characteristics. Ninety five bacteria  were isolated and characterized as lactic acid bacteria (Gram positive, catalase negative, non sporeforming and acid producing). Twenty four isolates of LAB demonstrated antimicrobial activity against Escherichia coli JCM 1649 and Salmonella enteritidis B2586, and three selected isolates, i.e. CCM011, CSP004, and CVM002 showed the highest inhibition activity. The isolates had characters of high cell surface hydrophobicity and inter-isolate coaggregation ability of LAB, high survival at low pH, high  phytase and protease activity (but no amylase and lipase activity), weak coaggregation with pathogen and no resistance to the examined antibiotics. The isolates were identified based on sequence analysis of 16S rRNA gene as Lactobacillus salivarius, however, each isolate had different profiles of sugar fermentation. Therefore the three LAB isolates had potential application as probiotics for chicken.Key words: Cemani chicken, gastrointestinal tract, lactic acid bacteria, probiotic
IDENTIFIKASI JENIS PIGMEN DAN UJI POTENSI ANTIOKSIDAN EKSTRAK PIGMEN BAKTERI RHODOCOCCUS SP HASIL ISOLASI DARI SEDIMEN SUMBER AIR PANAS GEDONG SONGO Zulfikar, Muhamad Fikri; Kusdiyantini, Endang; Jannah, Siti Nur
Jurnal Akademika Biologi Vol. 6 No. 4 Oktober 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Pigments are the coloring agents commonly used in the pharmaceutical, cosmetic, and food industries. This compound has the ability as antibacterial, anticancer, antibiotic and antioxidant. Pigment can be obtained synthetic and natural, natural pigments can be produced by plants, animals and microbes, one of which microbes is Rhodococcus sp. This study was conduct to identify the pigment found in Rhodococcus sp and measure the antioxidant activity of pigment produced by Rhodococcus sp. Growth and pigments production Rhodococcus sp was  grown on NB (Nutrient broth) medium with 96 hours incubation. This Growth was measured by dry weight cell. Identification of pigment using Thin Layer Chromatography and spectrophotometer UV-Vis. Measured of antioxidant activity Rhodococcus sp pigment using Carotene Bleaching methods. The results of pigments obtained from the identification by separation of pigment samples by thin layer chromatography resulted in 2 fractions on silica gel plate GF 250 with Rf values of 0.75 and 0.90. and analysis of UV-Vis spectrophotometers obtained optimum wavelengths 395 and 420 nm is beta-carotene pigments. Rhodococcus sp pigment measured its antioxidant activity with carotene bleaching method with the antioxidant activity value 51,24%.Keywords: Pigment, Rhodococcus sp, Pigment identification, antioxidant activity
KOMUNITAS RHIZOBAKTERIA TANAMAN TEH DENGAN APLIKASI FORMULA BIOIMUNIZER (CHRYSEOBACTERIUM SP DAN BACILLUS SP) BERDASARKAN GEN 16S RRNA Widaranti, Agnistisya; Jannah, Siti Nur; Kusumaningrum, Hermin Pancasakti; Susilowati, Dwi Ningsih
Jurnal Akademika Biologi Vol. 5 No. 4 Oktober 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Commodity of tea (Camellia sinensis) has an important role in the national economy, especially in the field of agro-industries. Based on data obtained from the Directorate General of Plantation, the tea plant productivity declined over the last few years. This is due to the pest attack which result in decreasing the productivity of the tea plant. Increased crop productivity of tea have been done, such as the use of herbicides and insecticides, but until now there is very little effort to increase the production of tea plants by the use of biological agents. Chryseobacterium sp and Bacillus sp are rhizobacteria in tea rhizosphere that could potentially be used as a biocontrol agent (bioimmunizer). The purpose of this study is to determine the community rhizobacteria in tea soil with the addition of bioimmunizer based on 16S rRNA gene using T-RFLP technique. The method used in this research is T-RFLP technique (Terminal Restriction Fragment Length Polymorphism) using enzymes Msp I and Rsa I. The calculated value are relative abundance, Shannon diversity index (H '), evenness index (E), and dominance index. The results of this study indicate that soil samples with the addition of bioimunizer consisting of Arthrobacter sp, Bacillus sp, Actinobacteria, and Chryseobacterium sp.Keyword: T-RFLP, Chryseobacterium sp, Bacillus sp
ISOLASI BAKTERIOFAG SPESIFIK PSEUDOMONAS SP. DA1 DARI BIOFILM PADA SISTEM PENGISIAN AIR MINUM ISI ULANG. Hayati, Zikra; Jannah, Siti Nur; Suprihadi, Agung
Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The social demand for drinking water increases both in urban and rural areas. The state encourages the development of Industrial Water Supply Depot (DAM) refills. problems occurred is no standard method for treating drinking water is sterileand lack of government supervision. This raises the issue of sanitation that is the presence of pathogenic bacteria that form biofilms in drinking water refill system. One of the bacterial pathogen is Pseudomonas. Bacteria Pseudomonas at refill drinking water can cause infections in the digestive tract. This study aims to find specific bacteriophage isolates of biofilm samples to infect bacteria Pseudomonas sp. on refill drinking water system. Isolation host Pseudomonas done in 2 ways dilution and filtration and grown on selective media Pseudomonas Isolation Agar. Isolates obtained were then characterized by gram staining and Kligler Iron Agar (KIA) test. Isolation of bacteriophages of biofilm done by bacteriophage amplification and filtration to obtain filtrate bacteriophage I and II. Test performed by using the host's infection Pseudomonas sp. DA1, Pseudomonas aeruginosa and Salmonella sp. (LIPI?s collection). The results showed that the drinking water refill depot contained positive isolates of Pseudomonas is Pseudomonas sp. DA1 and obtained isolate specific bakteriofag that can infect Pseudomonas sp. DA1. Bacteriophage titer calculation results on each sample at 3,0 x 105 PFU/ml in drinking water depot, 3,3 x 107 PFU/ml in the water product, and 9 x 107 PFU/ml water sources. Keyword: refill drinking water systems, biofilms, Pseudomonas sp DA1, bacteriophage
PENDIDIKAN ANAK MENURUT ZAKIAH DARADJAT Pratiwi, Firda; Hidayah, Adelina Nur; Khairani, Nelvi; Jannah, Siti Nur
POTENSIA: Jurnal Kependidikan Islam Vol 4, No 1 (2018): Juni
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/potensia.v4i1.4505

Abstract

Education is process that leads people to the good life and the uplifting of humanity in accordance with the basic capabilities (nature) and the his teaching ability (external influences). True education is that provides the opportunity for openness to influences from the outside world and the development of the student themselves. The purpose of  education is expected to from the persoality of a person into human beings with the pattern of taqwa to Allah SWT. Where the purpose of education is essentially that can humans in line with the incident. And aims to gain the solvation of the world and the hereafter. With the exixtence of education is expected to produce human beings who have a good personality in outword and inward who are able to devote charity and deeds to seek pleasure Allah. Education in the family has a strategic role and very decivise achievement of the quality of human resources. Implementation of family education is not just arolw as a routine and natural executor but acst as a responsible manager in laying a founation, weight direction and patterns of child life
ISOLASI, KARAKTERISASI AEROMONAS HYDROPHILA DAN DETEKSI GEN PENYEBAB PENYAKIT MOTILE AEROMONAS SEPTICEMIA (MAS) DENGAN 16S RRNA DAN AEROLYSIN PADA IKAN LELE (CLARIAS SP.) Muslikha, M; Pujiyanto, Sri; Jannah, Siti Nur; Novita, Hessy
Jurnal Akademika Biologi Vol. 5 No. 4 Oktober 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Aeromonas hydrophila merupakan bakteri Gram negatif yang banyak ditemukan di perairan dan dapat menyerang ikan. Selain itu, A. hydrophila menyebaban penyakit Motile Aeromonas Septiemia (MAS) yang menyerang beberapa organ dalam seperti hati, limpa dan ginjal. Isolat bakteri diisolasi dari ikan lele (Clarias sp.) yang berasal dari berbagai daerah seperti, Ciganjur, Sukamandi dan Citayam. Penelitian ini bertujuan untuk mengisolasi, karakterisasi dan deteksi gen patogen bakteri A. hydrophila penyebab penyakit MAS pada ikan lele. Beberapa pengujian yang dilakukan pada penelitian ini yaitu uji biokimia (pewarnaan Gram, uji oksidatif-fermentatif, uji katalase, uji oksidase, uji d-mannitol, uji TSA skim milk, uji Mac Conkey, dan uji novobiosin), uji deteksi gen patogen dilakukan secara molekuler dengan menggunakan primer 16S rRNA dan aerolysin. Berdasarkan hasil pengujian biokimia, A. hydrophila merupakan Gram negatif dengan sel berbentuk basil pendek, bersifat motil, positif menghasilkan enzim oksidase, enzim katalase dan positif oksidatif dan fermentatif, positif fermentasi laktosa. Hasil deteksi gen patogen menunjukkan isolat AH2 dan B9 memiliki gen faktor virulen yaitu, aerolysin. Isolat AH2 dan B9 menghasilkan gen aerolysin kembali pada deteksi gen hasil reisolasi dari postulat Koch. Kata kunci : Ikan Lele (Clarias sp.), Aeromonas hydrophila, Gen Faktor Virulen, Motile Aeromonas Septicemia (MAS).