HEDI INDRA JANUAR
Balai Besar Penelitian dan Pengembangan Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Published : 41 Documents
Articles

BIOACTIVE CEMBRANOID COMPOSITION IN THE SOFT CORAL OF SARCOPHYTON GLACCUM ON THE RESPONSE TO CHANGING PH Januar, Hedi Indra; Zamani, Neviaty Putri; Soedharma, Dedi; Chasanah, Ekowati
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 22, No 1 (2017): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (429.642 KB) | DOI: 10.14710/ik.ijms.22.1.25-30

Abstract

Soft coral is predicted to outcompete with hard coral in future ocean acidification scenarios. Beside the biological resilience shown in acidic conditions, soft corals ability to maintain or compete for space is shown to relate with their ability to produce cytotoxic cembranoid-type compounds. The aim of this study was to investigate composition of cytotoxic cembranoid compounds of Sarcophyton glaccum soft coral exposed to current and predicted future ocean acidification scenarios. Sarcophyton glaccum colonies were acclimated along a pH gradient to simulate predicted increases in ocean acidification: natural/current (pH 8,2), slight increase in acidification (pH 8.0 year-1 2060), and moderate increase in acidification (pH 7,8 year­-1 2100). Cembranoid composition was determined by quantitative Nuclear Magnetic Resonance spectroscopy while cytotoxic activity was determined against tumor cell lines. Results of the study showed cytotoxicity and sarcophytoxide (the most active cembranoid compound in observed Sarcophyton glaccum) were both found to be higher at pH 8,0. However, a further increase of acidification resulted on a reduction of both the cytotoxicity and sarcophytoxide production. This suggests that acidification pressures affect directly the defense system metabolism of Sarcophyton glaccum and that while they may be resilient to small decreases in pH, their ability to compete for space may be hampered by more pronounced changes. Keywords: Cembranoids; Cytotoxic; Sarcophyton glaccum; Seawater Acidification; Soft Coral.
STATISTICAL MULTI-METRIC BASED EUTROPHICATION INDEX: CASE OF STUDY AT BATAM MARINE RESERVE PARK Januar, Hedi Indra; Pratitis, Asri; Susilowati, Rini
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 24, No 4 (2019): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (666.388 KB) | DOI: 10.14710/ik.ijms.24.4.164-170

Abstract

Excessive human waste nutrients, which usually consist of nitrogenous and phosphate compounds, are known as the major environmental stresses in coastal waters. Therefore, monitoring of nutrients level is very important in marine reserve park. This study presents an application of multi-metric index in accessing the spatial and temporal level of a nutrient in surface water at Batam City Marine Reserve Park, Indonesia. Research had been done with seasonal and spatial zones on three major islands namely Petong, Abang, and Dedep. Water samples from each station were taken from the surface water. Phosphate, nitrate, nitrite, and ammonia, were analyzed in situ and chlorophyll was conducted in the laboratory by spectrophotometric method. Statistical multi-metric detected the average eutrophication index (EI) value between 0.2-0.7, which is mesotrophic to eutrophic. Petong and Abang zones were categorized as eutrophic, and Dadap was mesotrophic. Continuous nutrients contamination throughout seasonal may be derived from sedimentation and domestic run-off from human-populated islands in the middle and northern region. Therefore, ecosystem rehabilitation and mitigation of anthropogenic run-off are needed to optimise the conservation management. Moreover, Dadap zone as the area with the least anthropogenic pressures is potential to be the core of conservation area in Batam City marine reserve park.
CORRELATION DIVERSITY OF BACTERIA TO SECONDARY META BOLITES Nephthea spp. from SERIBU ISLANDS NATIONAL PARKS WATERS Patantis, Gintung; Januar, Hedi Indra; Chasanah, Ekowati
Widyariset Vol 15, No 3 (2012): Widyariset
Publisher : LIPI-Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (832.743 KB)

Abstract

Bioactive compounds from biota on coral reef is potentially of plasma nutfah that has high economic value.Their existence was influenced by the microbes which associated with them and the environmental conditions. Theaim of the research was to analyze the correlation between the diversity of bacteria and secondary metabolitesNephthea spp. collected from Seribu Islands National Parks (TNKpS). The analysis of bacteria diversity performedby Terminal Restriction Fragment Length Polymorphism (T-RFLP) and secondary metabolites used High PerformanceLiquid Chromatography (HPLC). The results of study showed that the range of diversity index (H), richness(R), and evenness index (E) of bacteria were 1,47–0,88; 13–3; 0,84–0,43. And the for the secondary metabolitesthe range were 2,21–1,12; 20-5; 0,95–0,63. Further study showed that there was a significant correlation (R =0.96 at p< 0.01) between bacteria and secondary metabolites group richness. The area which high diversity ofbacteria and secondary metabolites were within central and southern TNKpS waters. This results were in line withthe best water quality in these areas.
Cembranoids Content of Soft Coral Sarcophyton from Acidified Environment at Volcano Island, Indonesia Januar, Hedi Indra; Zamani, Neviaty Putri; Soedharma, Dedi; Chasanah, Ekowati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 1 (2017): May 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i1.276

Abstract

Cembranoid content in soft coral is known as a chemotype that relate with genotype and environment. This research aimed to characterize the cembranoid Sarcophyton soft coral from the reef that acidified by CO2 volcanic vents (pHT 7.8) at Volcano Islands waters, Banda-Neira (Indonesia), as a means of predicting the future impact of ocean acidification to the genetic diversity of Sarcophyton soft coral. 30 random colonies were taken, combined, and extracted with ethanol. Cembranoid isolation and identification had been done by high performance liquid chromatography and spectroscopic techniques. Results of the study found sarcophytol derivatives (sarcophytol A, 11,12-epoxy sarcophytol A, sarcophytol B, and sarcophytol M) as the only chemotype in the sample. This may suggest low genetic diversity in the observed Sarcophyton sample. Therefore, it may suggest that even soft coral is known to be resilient to future acidification pressures, the genetic diversity or the production of diverse cytotoxic metabolite may be hampered due to ocean acidification in future climate change adaptation.
Fatty Acid Profile, Carotenoid Content, and In Vitro Anticancer Activity of Karimunjawa and Lampung Sea Cucumber Chasanah, Ekowati; Fawzya, Yusro Nuri; Tarman, Kustiariyah; Januar, Hedi Indra; Nursid, Muhammad
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 3 (2016): December 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i3.269

Abstract

Fatty acids and carotenoid has been known as an anticancer agent on both preventing and treating cancer disease. This study was conducted to analyze the fatty acid profile, carotenoid and in vitro anticancer activity of 12 sea cucumber harvested from Karimunjawa and Lampung waters. The aim of the study was to determin the potency of sea cucumbers as raw material for nutraceutical products. Fatty acid profile and carotenoid content were characterized by gas chromatography and spectrophotometry techniques, while in vitro anticancer activity was assessed by MTT assay against cervix (HeLa), breast (T47D and MCF-7) and colon (WiDR) cancer cells. Results of the study showed polyunsaturated fatty acid (PUFA) dominated the composition of fatty acids in the samples from both locations. Holothuria sp. was detected to contain the highest amount of carotenoid. Furthermore, the highest in vitro anticancer activity was detected also in the sample of Holothuria sp. The activity of 30 ppm Holothuria sp. extract against HeLa cell was detected to be almost equal to the 5 ppm doxorubicin control. Concentration of 5 ppm Holothuria sp. extract also showed positive result in killing 50% of MCF-7 and T47D, but capable to 100% kill HeLa and WiDR cells. At concentration of 25 ppm, the extract was able to kill all the 4 cells tested. Statistical analysis showed the amount of carotenoid and two particular fatty acid compounds (docosadienoic and eicosapentaenoic acid) significantly (P<0.05) contributed to the cytotoxic activity that was found in the sea cucumber samples. Those compounds were found in highest concentration from Holothuria sp harvested from Lampung waters, thus being the most prospective raw material for nutraceutical or functional food ingredient with anticancer potency.
Correlation between fucoxanthin contents in Turbinaria sp.and sea water nutrients at Binuangeun and Krakal Coasts Januar, Hedi Indra; Wikanta, Thamrin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 6, No 1 (2011): May 2011
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v6i1.57

Abstract

Fucoxanthin pigment is a potential compound in nutraceutical. It is commonly found in algaesuch as in Turbinaria sp.,a very abundant species in Indonesian coastal. To maximize theproduction of fucoxanthin for the development of nutraceutical industry in the coastal area, it isimportant to identify the optimal environmental parameters for its biosynthesis. It has been reportedthat nitrogen and or phosphate nutrients affect the growth of algae. Therefore, this paper aimed toobtain an optimal environment for Turbinaria sp. to produce fucoxanthin based on correlationanalysis. Correlated data were fucoxanthin isolated from Turbinaria sp.that was collected fromKrakal (Yogyakarta) and Binuangeun (Banten) coasts. Fucoxanthin contents in each sample werecompared based on the peak areas in chromatogram after 1 mg injection of n-hexane extract ofT. decurrrens in the HPLC system. The analysis results showed that the peak areas of fucoxanthinwere significantly different among sampling locations (P = 7.99 x 10-6), sampling periods (P = 6 x10-3) and interaction of both (P = 3 x 10-3). Whereas the correlation analysis revealed that interactionbetween phosphate and temperature could be a predictor that strongly (R = 0.986) and significantlycorrelated (P = 1.09 x 10-7) with the peak areas of fucoxanthin (R2= 96.5%). These results were inline with growth limiting factor in seawater and the function of fucoxanthin as ultraviolet protector.Based on these results, it is suggested that the suitable location for fucoxanthin production is in thearea of agricultural run-off that contains an elevated concentration of dissolved phosphate.
STUDY ON THE EFFECT OF POLLUTANTS ON THE PRODUCTION OF AAPTAMINES AND THE CYTOTOXICITY OF CRUDE EXTRACT FROM Aaptos suberitoides Dewi, Ariyanti Suhita; Hadi, Tri Aryono; Januar, Hedi Indra; Pratitis, Asri; Chasanah, Ekowati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 7, No 3 (2012): December 2012
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v7i3.4

Abstract

This experiment was conducted to study the effects of anthropogenic stressor on the spatialvariability of secondary metabolites from marine sponge Aaptos suberitoides. Samplings wereconducted at 7 sites in Marine National Park of Thousand Islands that are extended within 30 kmoff Jakarta bay on late February 2011. Sponges were collected and quantified by means of liquidchromatography coupled with photo-diode array detection, whereas, cytotoxicity of sponges extractswas determined against T47D (breast) cancer cell lines. Results showed that the spatialproduction of aaptamine and isoaaptamine did not correlate with the quality of their surroundinghabitat, despite nitrite and nitrate levels significantly affected the bioactivity of crude extracts.
PERBANDINGAN BEBERAPA METODE ANALISIS HISTAMIN UNTUK PRODUK PERIKANAN Januar, Hedi Indra
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 4, No 2 (2009): August 2009
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v4i2.150

Abstract

Studi ini  bertujuan  untuk  membandingkan  beberapa  metode dalam penentuan kadar amina  biogenik histamin. Diketahui, kadar histamin merupakan salah satu parameter yang penting  sebagai  standar  kualitas  produk  perikanan. Metode yang dibandingkan meliputi metode menggunakan spektrofluorometri, High Performance Liquid Chromatography (HPLC), Enzyme Linked Immunosorbent  Assay (ELISA), dan Capillary Electrophoresis/Capillary Zone Electrophoresis (CE/CZE). Hasil perbandingan menunjukkan bahwa baik dari sisi ketidakpastian yang mungkin ditimbulkan dari metodenya serta hasil uji kelayakan laboratorium di Eropa, metode HPLC derivatisasi post-kolom merupakan metode yang optimal saat ini untuk menentukan kadar histamin secara kuantitatif. Akan tetapi, untuk pertimbangan efisiensi waktu, maka studi ini mengusulkan bahwa penggabungan metode ELISA kualitatif dan HPLC kuantitatif sangat baik  dijadikan sebagai standar metode penentuan histamin di laboratorium pengujian produk perikanan.
Chemical Composition and Fatty Acid Profile of Some Indonesian Sea Cucumbers Fawzya, Yusro Nuri; Januar, Hedi Indra; Susilowati, Rini; Chasanah, Ekowati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 1 (2015): May 2015
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v10i1.118

Abstract

Indonesia has a great sea cucumber resource which is useful as functional food. Even though much information about the biopotency of sea cucumbers have been published, however, studies on nutrition value of Indonesian sea cucumbers are still beneficial due to the great diversity and uniqueness of each species. The present work was  intended to obtain information about chemical composition and fatty acid profile of 4 (four) types of sea cucumbers which are frequently found in Halmahera water, North Maluku (Molucca),  one  of  sea  cucumbers  producing area  in Indonesia. The samples coded as  H-03, H-04, H-05 and H-10, were identified as Bohadschia  argus, Holothuria  fuscogilva, Thelenota  ananas, and Actinophyga lecanora. All sea cucumbers showed high protein content, more than 60% (drybase/db), except for T. ananas which had the lowest protein content (48.26% db). As for fats, T. ananas showed the highest amount (2.35%db) with dominated saturated fatty acids (SFA). On the other side, A. lecanora showed higher value of PUFA compared to SFA and MUFA.  Palmitic acid (C16:0) was the most abundant SFA in the most samples with the amount of 0.49–4.9 mg/g sample. Among PUFA, eicosapentaenoic acid/EPA (C20:5n3) was detected, and eicosatrienoic acid (C20:3n6) was relatively higher than the others. The ratio of w6/w3 fatty acid was 1.2–3.2 showing that total w6 fatty acid was higher than total w3 fatty acid. However, this value is safe enough to protect against chronic and degenerative diseases.
CYTOTOXIC SATURATED FATTY ACIDS FROM THE INDONESIAN SEA CUCUMBER Holothuria sp. Januar, Hedi Indra; Nursid, Muhammad; Chasanah, Ekowati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 9, No 1 (2014): May 2014
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v9i1.69

Abstract

Sea cucumbers are one of Indonesia’s marine organism with the potential to be developed as possible herbal medicines. In a preliminary study of cytotoxic activity of ethanol extracts of 14Indonesian sea cucumber species, the most active extract came from the Holothuria sp. Thecurrent research aimed to identify the major cytotoxic component in the sea cucumber Holothuriasp., that yielded the most active extract. The samples were collected from South Lampung beach.Isolation of the cytotoxic component was done employing liquid flash and preparative reversedphase (C 18) chromatography. Cytotoxic ity evalu atio n of fractio ns collected durin g thechromatography was conducted using the MCF-7 tumor cell line. Elucidation of the structure ofmost active isolate was done by NMR (Nuclear Magnetic Resonance) spectroscopy and GC-FID(Gas-Chromatography-Flame Ionisation Detector) analysis. The results of these analyses showedthe most active compound to be stearic acid; IC 50towards MCF-7 cells 10.32 ppm.